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1.
Kairy Dharali Pujols Luis Osorio Elsy Paola Carrillo Wisdom Wardy Damir Dennis Torrico Hong Kyoon No José Andrés Herrera Corredor Witoon Prinyawiwatkul 《International Journal of Food Science & Technology》2014,49(5):1383-1390
Effects of α‐ and β‐chitosan (CH), soybean oil (SO) and their emulsions (CH:SO = 2:3) as coating materials on selected internal quality and sensory properties of eggs were evaluated during 5 weeks storage at 25 °C. After 3 weeks of storage, α‐ and β‐CH‐coated eggs changed to B grade, while SO‐ and emulsion‐coated eggs preserved grade A quality. Weight loss of eggs coated with SO and CH:SO emulsions was <2.0% vs. 5.3–5.8% for noncoated and CH‐coated eggs after 5 weeks of storage. β‐CH (0.9%) maintained lower weight loss of eggs than α‐CH (1.2%) only at 1‐week storage. Albumen pH of eggs coated with SO and CH:SO emulsions decreased progressively throughout storage. Eggs coated with β‐CH:SO emulsion and SO were significantly glossier than noncoated eggs. Consumers indicated positive purchase intent (69.17–76.67%) for all coated eggs. Overall, α‐CH:SO and β‐CH:SO emulsions extended egg shelf life by at least 3 weeks during room temperature storage. 相似文献
2.
Damir D. Torrico Wannita Jirangrat Hong Kyoon No Witoon Prinyawiwatkul Beilei Ge Dennis Ingram 《International Journal of Food Science & Technology》2010,45(11):2241-2249
Effects of mineral oil (MO), chitosan solution (CH) and their emulsions (MO:CH = 75:25, 50:50, and 25:75 ratios) as coating materials in preserving internal quality of eggs were evaluated during a 5‐weeks storage at 25 °C. Consumers (n = 109) evaluated surface properties and purchase intent of freshly coated eggs. As storage time increased, Haugh unit and yolk index values decreased whereas weight loss increased. Noncoated eggs rapidly changed from AA to B and C grades after 1 and 3 weeks, respectively. However, all emulsion‐coated eggs maintained their A‐grade quality for 4 weeks. Compared with noncoated eggs, all emulsion coatings reduced weight loss of eggs by at least seven times (0.88–1.03% vs. 7.14%). Only MO:CH = 25:75 emulsion‐coated eggs were not sensorially glossier than noncoated eggs. All emulsion‐coated eggs had >80% positive purchase intent and were negative for Salmonella. This study demonstrated that MO:CH emulsion coatings preserved internal quality and prolonged shelf life of eggs. 相似文献
3.
Damir Dennis Torrico Hong Kyoon No Sujinda Sriwattana Dennis Ingram Witoon Prinyawiwatkul 《International Journal of Food Science & Technology》2011,46(9):1783-1792
Effects of mineral oil (MO) and mineral oil–chitosan emulsion (MO:CH = 25:75) as coatings on internal quality and shelf‐life of eggs were evaluated during 5‐week storage at 25 °C. Eggs with three different initial albumen qualities [Haugh unit (HU): H = 87.8, M = 75.6 and L = 70.9] were evaluated. As storage time increased, HU and yolk index values decreased whereas weight loss increased. Coating with MO and/or 25:75 MO:CH emulsion could preserve the internal quality for at least 4 more weeks for H‐eggs and at least 3 more weeks for M and/or L‐eggs, all with weight losses <0.92%. All coated eggs had >70% positive purchase intent, and their colour differences at week 0 could not be detected by naked human eye (ΔE* < 3.0, noncoated eggs as reference). Consumers significantly differentiated freshly MO‐coated from noncoated eggs on overall surface appearance. This study demonstrated that MO and 25:75 MO:CH emulsion coatings could preserve internal quality and prolong shelf‐life of eggs. 相似文献
4.
Wisdom Wardy Damir D. Torrico José A. Herrera Corredor Hong Kyoon No Xiumei Zhang Zhimin Xu Witoon Prinyawiwatkul 《International Journal of Food Science & Technology》2013,48(6):1148-1156
Effects of soybean oil (SO), chitosan solution (CH) and their emulsions (SO:CH = 60:40, 50:50 and 40:60 ratios) as coatings on internal quality of eggs stored at 25 and 4 °C, respectively, for 7 and 20 weeks, were evaluated. Eggs coated with SO and SO:CH emulsions maintained grade AA and/or A quality up to 7 weeks at 25 °C and 20 weeks at 4 °C, while noncoated eggs changed from AA to B grade after 2 weeks at 25 °C. Compared with noncoated eggs, shelf‐life of eggs stored at 25 °C was extended for 5 weeks by all SO:CH emulsions. Weight loss of eggs coated with SO:CH emulsions was <3% after 7 weeks at 25 °C and <5% after 20 weeks at 4 °C. SO:CH emulsion is alternatively an effective coating with possible shorter drying times for reducing weight loss and preserving the internal quality of eggs. 相似文献
5.
Effects of storage of chitosan (CH) solution on physico-functional properties and consumer perception of CH-coated eggs were evaluated during 5- and 15-weeks storage at 25 °C and 4 °C, respectively. Seven treatments [CH0 (freshly-prepared), CH1 (stored for 1-week at 25 °C), CH1R (1-week, 4 °C), CH3 (3-weeks, 25 °C), CH3R (3-weeks, 4 °C), CH5 (5-weeks, 25 °C), CH5R (5-weeks, 4 °C)] were applied on eggshell. After 5-weeks storage, CH-solution viscosity decreased by 2.56 and 4.6 times, respectively, at 4 °C and 25 °C while pH slightly increased. CH0 preserved grade-A quality for 4-weeks vs. 1-week for noncoated eggs at 25 °C. After 5-weeks, weight loss (%) of coated eggs at 25 °C (6.04–5.59) was lower than that of noncoated eggs (7.44) but higher than that of all eggs at 4 °C (2.93–2.46). Albumen pH increased while emulsion capacity decreased with increased storage time; however, both were insignificantly affected by CH viscosity. Consumers perceived CH0- and CH1R-eggshell to be glossier than noncoated eggs after 5-weeks. Purchase intent was higher for CH-coated eggs (72–77.3%) than for noncoated eggs (61.3%). Overall, viscosity changes of CH-coating solution had lesser impact on quality of CH-coated eggs than did storage temperature/time of eggs. 相似文献
6.
Damir Dennis Torrico Wisdom Wardy Kennet Mariano Carabante Kairy Dharali Pujols Zhimin Xu Hong Kyoon No Witoon Prinyawiwatkul 《LWT》2014
Effects of mineral oil:chitosan (MO:CH at 25:75) emulsions prepared with four different emulsifiers (2 water- and 2 oil-miscible) as coatings on the internal quality (weight loss, Haugh unit, yolk index, and albumen pH) of coated eggs were evaluated during 5 weeks at 25 ± 2 °C and 20 weeks at 4 ± 2 °C. Eggs with two initial albumen qualities [Haugh unit (HU): H = 87.8 and L = 70.9] were used. At 25 ± 2 °C, Haugh unit, yolk index, and albumen pH of all coated eggs decreased with increased storage time. Coated H- and L-eggs maintained an A-grade up to 4 weeks and 1 week, respectively. Weight loss of all coated eggs remained below 1.35% after 5 weeks of storage at 25 ± 2 °C. All coated eggs maintained an A-grade with less than 2.5% weight loss during 20 weeks of storage at 4 ± 2 °C. Emulsifier types marginally affected the internal quality of coated eggs regardless of storage temperatures. 相似文献
7.
Torrico DD No HK Prinyawiwatkul W Janes M Corredor JA Osorio LF 《Journal of food science》2011,76(4):S262-S268
Effects of mineral oil (MO) and 4 emulsions (prepared with different emulsifier types) of MO and chitosan solution (CH) at a fixed ratio of MO:CH = 25:75 as coating materials in preserving the internal quality of eggs were evaluated during 5 wk at 25 °C and 20 wk at 4 °C. Generally, as storage time increased, Haugh unit and yolk index values decreased whereas weight loss increased. However, MO and/or 4 emulsion coatings minimized the weight loss (<1.5%) and preserved the albumen and yolk quality of eggs (with the final B grade) for at least 3 wk longer than those observed for noncoated eggs at 25 °C. At 4 °C, all coated eggs changed from AA to A grade after 5 wk and they maintained this grade for 10 wk (5 wk longer than that of noncoated eggs). Although refrigeration (4 °C) alone could maintain the B grade of noncoated eggs for up to 20 wk, coating treatments were necessary to keep the weight loss below 2%. Compared with 4 °C, the increasing weight loss showed stronger negative correlation (P < 0.01) with the decreasing Haugh unit (-0.46 to -0.89) and yolk index (-0.36 to -0.89) at 25 °C. The emulsifier type used in this study generally did not affect the internal quality of eggs. Salmonella spp. detection was negative for all coated and noncoated eggs. This study demonstrated that MO and MO:CH emulsion coatings preserved the internal quality, prolonged the shelf-life, and minimized weight loss (<2%) of eggs. 相似文献
8.
Effects of mineral oil coating on internal quality of chicken eggs under refrigerated storage 总被引:1,自引:0,他引:1
Wannita Jirangrat Damir Dennis Torrico June No Hong Kyoon No Witoon Prinyawiwatkul 《International Journal of Food Science & Technology》2010,45(3):490-495
The selected internal qualities (weight loss, Haugh unit, yolk index, and albumen pH) of noncoated and mineral oil‐coated chicken eggs during 15 weeks of storage at 4 °C and/or during 5 weeks of storage at 25 °C were evaluated. Results indicated that, without refrigeration, the noncoated and mineral oil‐coated eggs rapidly changed from AA to C and B grades as measured by Haugh unit, respectively, after 5 weeks of storage. However, the AA quality of the noncoated eggs could be maintained under refrigerated storage (4 °C) for at least 5 weeks. The mineral oil coating and refrigerated storage (4 °C) synergistically minimised weight loss and preserved the albumen and yolk qualities of chicken eggs during a long‐term storage. At 4 °C, the mineral oil‐coated eggs preserved the initial AA grade for at least 15 weeks with l.19% weight loss. 相似文献
9.
Wisdom Wardy Damir D. Torrico Hong Kyoon No Witoon Prinyawiwatkul Firibu K. Saalia 《International Journal of Food Science & Technology》2010,45(12):2659-2668
Effects of chitosan, whey protein concentrate (WPC), mineral oil (MO) and/or soybean oil (SO) coating on egg quality were compared at 25 and 4 °C, respectively, during 5 and 20 weeks of storage. Storage time and temperature, and type of coating significantly affected Haugh unit, yolk index, weight loss, albumen pH and emulsifying capacity. Shelf life was extended 4 weeks by MO and SO and 2 weeks by chitosan and WPC longer than that observed for noncoated eggs at 25 °C. MO‐ and SO‐coated eggs maintained AA grade for 20 weeks at 4 °C. Weight loss of SO‐coated eggs was <1% after 5 weeks at 25 °C and after 20 weeks at 4 °C. Yolk index and emulsifying capacity were more correlated at 25 °C than at 4 °C. MO and SO were more effective coating materials, with SO providing a more cost‐effective coating for extending egg shelf life. 相似文献
10.
Sinee Nongtaodum Anuvat Jangchud Kamolwan Jangchud Pisit Dhamvithee Hong Kyoon No Witoon Prinyawiwatkul 《Journal of food science》2013,78(2):S329-S335
Four (coconut, palm, rice bran, and soybean) edible oils and glycerol were applied on eggshell. All noncoated and coated eggs were stored for 5 wk at 25 ± 2 °C and drawn weekly for quality evaluation. All oil coatings were more effective in preserving internal quality of eggs than was glycerol coating. As storage time increased, the preservative effects of edible oil coating on weight loss, and albumen and yolk quality were significantly noticed. Oil‐coated eggs had significantly lower weight loss (<0.43%) than did noncoated (3.87%) and glycerol‐coated (3.73%) eggs after 5 wk of storage. Based on the Haugh unit, oil‐coated eggs maintained AA grade up to 3 wk. After 5 wk of storage, noncoated, glycerol‐coated, and oil‐coated eggs changed from AA grade to below B, below B and A grade, respectively. The albumen pH of noncoated and glycerol‐coated eggs considerably increased from 8.23 to 9.51 and 9.42, respectively, while those of oil‐coated eggs either maintained or slightly increased to 8.32. The albumen viscosity of all eggs decreased with increased storage time. Consumers (N = 120) could differentiate surface glossiness of oil‐coated eggs from uncoated eggs (R‐index of 81.42% to 86.99%). All oil‐coated eggs were acceptable for surface glossiness (liking scores of 6.22 to 6.77) and surface odor (liking scores of 6.20 to 6.55) with overall liking scores of 6.34 to 7.03. Overall, this study demonstrated that edible oil (coconut, palm, rice bran, and soybean) coating could preserve internal quality of eggs (maintaining grade A) at least 4 wk longer than noncoated eggs. Practical Application: Freshness is a major contribution to the egg quality. The internal quality of eggs begins to deteriorate after they have been laid due to loss of moisture and carbon dioxide via the eggshell pores. Refrigeration is very effective in preserving egg quality. Surface coating is an alternative method to preserve egg quality, although it is much less effective than refrigeration. This study demonstrated that coconut, rice bran, soybean, and palm oils, which are abundant and commonly consumed in many parts of the world, could preserve the internal quality and reduce weight loss of oil‐coated eggs during room temperature storage. 相似文献
11.
Selected Quality and Shelf Life of Eggs Coated with Mineral Oil with Different Viscosities 总被引:1,自引:0,他引:1
Pamarin Waimaleongora-Ek Karen M. Garcia Hong Kyoon No Witoon Prinyawiwatkul Dennis R. Ingram 《Journal of food science》2009,74(9):S423-S429
ABSTRACT: Selected quality and shelf life of eggs coated with mineral oil having 6 different viscosities (7, 11, 14, 18, 22, and 26 cP) were evaluated during 5 wk of storage at 25 °C. As the storage time increased, weight loss and albumen pH increased whereas Haugh unit and yolk index values decreased. After 5 wk of storage, eggs coated with 11, 14, 18, 22, or 26 cP oil possessed better quality than the control noncoated eggs and eggs coated with 7 cP oil. Oil coating, irrespective of viscosities, did not improve the emulsion capacity. There was an observable trend that coating with 26 cP oil was more effective in preventing weight loss and in maintaining the Haugh unit of eggs compared with coating with other viscosities of mineral oil. Based on the Haugh unit, the grade of noncoated eggs changed from "AA" at 0 wk to "C" after 3 wk whereas that of 26 cP oil-coated eggs from "AA" at 0 wk to "A" at 3 wk and "B" at 5 wk of storage. Coating with 26 cP oil reduced the weight loss of eggs by more than 10 times (0.85% compared with 8.78%) and extended the shelf life of eggs by at least 3 more weeks compared with the noncoated eggs. 相似文献
12.
Su Hyun Kim Dal Kyoung Youn Hong Kyoon No Sang Won Choi & Witoon Prinyawiwatkul 《International Journal of Food Science & Technology》2009,44(7):1351-1359
Effects of chitosan coating and storage positions (small-end down, small-end up or horizontal) on internal quality and shelf life of eggs were evaluated during 5 weeks of storage at 25 °C. Compared with noncoated eggs, all chitosan-coated eggs, irrespective of storage positions, had significantly lesser weight loss, higher Haugh units and higher yolk index throughout the storage. Chitosan coating, irrespective of storage positions, extended the egg shelf life by at least 3 weeks at 25 °C compared with noncoated eggs. Noncoated and chitosan-coated eggs placed small-end down tentatively showed better quality than eggs placed small-end up after 3 and 4 weeks of storage. After 5 weeks of storage, storage positions did not significantly affect quality of noncoated and chitosan-coated eggs. There were no notable differences in total amino acid content of the albumen and fatty acid composition of the yolk between noncoated and chitosan-coated eggs after 5 weeks of storage. 相似文献
13.
Chitosan Coating Improves Shelf Life of Eggs 总被引:5,自引:0,他引:5
S. Bhale H.K. No W. Prinyawiwatkul A.J. Farr K. Nadarajah S.P. Meyers 《Journal of food science》2003,68(7):2378-2383
ABSTRACT: Internal and sensory quality of eggs coated with chitosan was evaluated during a 5-wk storage at 25 °C. Three chitosans with high (HMw, 1100 KDa), medium (MMw, 746 KDa), and low (LMw, 470 KDa) molecular weight were used to prepare coating solutions. Coating with LMw chitosan was more effective in preventing weight loss than with MMw and HMw chitosans. The Haugh unit and yolk index values indicated that the albumen and yolk quality of coated eggs can be preserved up to 5 wk at 25 °C, which is at least 3 wk longer than observed for the control noncoated eggs. Based on external quality, consumers could not differentiate the coated eggs from the control noncoated eggs. Overall acceptability of all coated eggs was not different from the control and commercial eggs. 相似文献
14.
Selected internal quality and shelf life of eggs coated with oils from differences sources (mineral oil, canola oil, corn oil, grape seed oil, olive oil, soybean oil, and sunflower oil) were evaluated during 5 wk of storage at 25 °C. As the storage time increased, weight loss increased whereas Haugh unit and yolk index values decreased. Throughout the 5 wk of storage, eggs coated with oils, regardless of oil sources, possessed better albumen and yolk quality than the control noncoated eggs. Oil coating minimized weight loss of eggs (<0.8%) compared with that (7.26%) of the noncoated eggs after 5 wk of storage at 25 °C. No significant differences in internal quality (weight loss, Haugh unit, yolk index, and albumen pH) were generally observed among oil-coated eggs during 5 wk of storage. Based on the Haugh unit, the grade of noncoated eggs changed from AA at 0 wk to A at 1 wk and to B after 3 wk whereas that of oil-coated eggs from AA at 0 wk to A at 4 wk and maintained A grade until 5 wk. This study demonstrated that oil coating, irrespective of oil sources, preserved the internal quality, minimized weight loss (<0.8%), and extended the shelf life of eggs by at least 3 wk longer than observed for the noncoated eggs at 25 °C storage. Soybean oil was a more practical option as a coating material for eggs due to its low cost. PRACTICAL APPLICATION: Eggs are highly perishable and susceptible to internal quality deterioration when stored above 7 °C. Refrigeration of eggs may be seldom practiced in some developing regions of the world. Therefore, an alternative method, that is inexpensive yet effective, to preserve the internal quality of eggs and to prevent microbial contamination is needed. Oil coating has been proven to preserve the internal quality, prolong shelf life, and minimize weight loss of eggs. This study demonstrated that, compared with other vegetable oils, soybean oil was a more practical option as a coating material for eggs during 5 wk of storage at 25 °C due to its low cost. 相似文献
15.
Cengiz Caner 《Journal of the science of food and agriculture》2005,85(11):1897-1902
Effects of the various coatings (whey protein isolate, chitosan and shellac) on fresh eggs quality were evaluated based on the interior quality and sensory evaluation during 4 weeks of storage. During storage, all egg weights and albumen heights decreased and albumen pH increased. The lowest weight loss (0.75%) was observed in shellac‐coated eggs. Eggs coated with chitosan and whey protein also had significantly lower weight loss than uncoated (UC) eggs (p < 0.05). The albumen pH of the UC eggs was significantly higher than that of coated eggs and increased during storage time. The Haugh unit and yolk‐index values of all coated eggs were significantly higher than those of UC. Among the coated eggs, the shellac eggs had the highest value of Haugh unit and yolk index. Chitosan and shellac effectively maintained grade ‘A’ eggs for at least 2 weeks more than control and 1 week more than whey protein isolate. On the basis of sensory evaluation, shellac has highest glossiness, but lowest general acceptability. Eggs coated with whey protein had significantly higher general acceptability. Yolk lightness (L*)(a*) and (b*) of coated eggs were not different from UC after 4 weeks. The values of color differences were similar to controls. The study demonstrated that various coatings improved the shelf life of eggs. Copyright © 2005 Society of Chemical Industry 相似文献
16.
Efficacy of Antimicrobial Pullulan‐Based Coating to Improve Internal Quality and Shelf‐Life of Chicken Eggs During Storage
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Mohamed K. Morsy Ashraf M. Sharoba Hassan H. Khalaf Hassan H. El‐Tanahy Catherine N. Cutter 《Journal of food science》2015,80(5):M1066-M1074
There has been a growing interest in the use of natural materials as a delivery mechanism for antimicrobials and coatings in foods. The aim of the present study was to evaluate the effectiveness of pullulan coatings to improve internal quality and shelf‐life of fresh eggs during 10 wk of storage at 25 and 4 °C. Three treatments of eggs were evaluated as follows; non‐coated (control; C), coated with pullulan (P), and coated with pullulan containing nisin (N). The effects of the pullulan coatings on microbiological qualities, physical properties, and freshness parameters were investigated and compared with non‐coated eggs. For non‐coated eggs, as storage time increased, yolk index, albumen index, and Haugh unit value decreased and weight loss increased. However, pullulan coatings (P or N) minimized weight loss (<1.5%) and preserved the albumen and yolk quality of eggs (with a final B grade) 3 wk longer than non‐coated eggs at 25 °C. At 4 °C, both P‐ and N‐coated eggs went from AA to A grade after 9 wk and maintained the grade for 10 wk (4 wk longer than that of non‐coated eggs). This study is the first to demonstrate that pullulan coatings can preserve the internal quality, prolong the shelf‐life, and minimize weight loss of fresh eggs. 相似文献
17.
Effect of Plasticizer Concentration and Solvent Types on Shelf-life of Eggs Coated with Chitosan 总被引:2,自引:0,他引:2
Su Hyun Kim Hong Kyoon No Soon Dong Kim Witoon Prinyawiwatkul 《Journal of food science》2006,71(4):S349-S353
ABSTRACT: Effects of plasticizer concentrations (0, 0.5, 1.0, 1.5, and 2.0% glycerol) and solvent types (1% acetic and 1% lactic acid) on internal quality of eggs coated with 2% chitosan solution were evaluated during 5 wk of storage at 25 °C. In comparison of plasticizer concentrations, eggs coated with chitosan dissolved in acetic acid containing 2% glycerol showed significant reduction in weight loss compared with the noncoated eggs during 5 wk of storage. At 2% glycerol, the Haugh unit and yolk index values suggested that chitosan-coated eggs can be preserved for at least 3 wk longer than the control noncoated eggs during 5 wk of storage at 25 °C. Use of acetic acid rather than lactic acid as a chitosan solvent was more advantageous in view of shelf-life extension of eggs. 相似文献
18.
Z. R. Howard R. W. Moore I. B. Zabala Diaz W. K. Kim S. G. Birkhold J. A. Byrd L. F. Kubena D. J. Nisbet S. C. Ricke 《Journal für Verbraucherschutz und Lebensmittelsicherheit》2006,1(1):28-32
Salmonella enterica serovar Typhimurium has been isolated from commercial egg production facilities in the United States. Given its importance
as a causative organism for food-borne salmonellosis, identifying approximate timelines for bacterial invasion of the egg
is needed. The objective of this study was to examine net growth of S. Typhimurium in egg components over time. In trial 1 eggs were collected over a 24 hour period from a flock of single comb
white leghorn hens while in trial 2 eggs were picked up from a commercial laying source once a week over the course of eight
weeks and stored. Eggs were held at refrigeration temperature and each week, subsets of eggs were cracked, separated into
yolk and albumen components, and inoculated with 108 CFU/ml of novobiocin and nalidixic acid (NO/NA) resistant S. Typhimurium onto the vitelline membrane of the egg. Yolks were then covered with albumen. Eggs were incubated for twenty-four
hours at 25°C. After incubation eggs were again separated into albumen, yolk, and vitelline membrane samples. In trial 1,
S. Typhimurium net growth occurred in albumen by the second week and continued from 4 to 8 weeks while in trial 2 net growth
only occurred at week 5 and 7. S. Typhimurium net growth on vitelline membranes occurred by 2 weeks and continued from 4 to 8 weeks in trial 1 while no net
growth occurred in trial 2 over the 8 week period. Yolk samples showed no net increases in S. Typhimurium populations over the 8 week period.
An erratum to this article is available at . 相似文献
19.
Z. R. Howard R. W. Moore I. B. Zabala Diaz W. K. Kim S. G. Birkhold J. A. Byrd L. F. Kubena D. J. Nisbet S. C. Ricke 《Journal für Verbraucherschutz und Lebensmittelsicherheit》2006,58(5):28-32
Salmonella enterica serovar Typhimurium has been isolated from commercial egg production facilities in the United States. Given its importance
as a causative organism for food-borne salmonellosis, identifying approximate timelines for bacterial invasion of the egg
is needed. The objective of this study was to examine net growth of S. Typhimurium in egg components over time. In trial 1 eggs were collected over a 24 hour period from a flock of single comb
white leghorn hens while in trial 2 eggs were picked up from a commercial laying source once a week over the course of eight
weeks and stored. Eggs were held at refrigeration temperature and each week, subsets of eggs were cracked, separated into
yolk and albumen components, and inoculated with 108 CFU/ml of novobiocin and nalidixic acid (NO/NA) resistant S. Typhimurium onto the vitelline membrane of the egg. Yolks were then covered with albumen. Eggs were incubated for twenty-four
hours at 25°C. After incubation eggs were again separated into albumen, yolk, and vitelline membrane samples. In trial 1,
S. Typhimurium net growth occurred in albumen by the second week and continued from 4 to 8 weeks while in trial 2 net growth
only occurred at week 5 and 7. S. Typhimurium net growth on vitelline membranes occurred by 2 weeks and continued from 4 to 8 weeks in trial 1 while no net
growth occurred in trial 2 over the 8 week period. Yolk samples showed no net increases in S. Typhimurium populations over the 8 week period. 相似文献
20.
The study evaluates the efficacy of three common market-grade culinary vegetable oils—groundnut oil, cottonseed oil and coconut oil—in preserving the internal quality of freshly laid eggs coated with them and stored under tropical room conditions of 25–32°C and 40–85% r.h. for 36 days. All three vegetable oils significantly (P<0.01) limited diurnal weight losses of the eggs to 0.013–0.016 g as against 0.186 ± 0.013 g for the untreated eggs over 36 days storage. Liquefaction of the yolk and albumen was also significantly reduced (P<0.05) such that the albumen pH of the oiled eggs increased from 7.65 to only 8.35–8.65 after 36 days when the untreated eggs had an albumen pH of 9.80. Subsequently, the yolk index of oiled eggs dropped by only 0.10–0.13 after 36 days and remained significantly superior (P<0.001) to that of the untreated eggs which had dropped from an initial value of 0.47 to 0.11. These preservative effects of the vegetable oils may be attributable to their fatty acid component, rather than their glycerol which showed no significant (P>0.05) improvement over untreated eggs. 相似文献