红脚艾和汤阴北艾精油的化学成分及抗菌活性

通过水蒸气蒸馏法（SD）和超临界流体萃取-分子蒸馏法（SFE-MD）从罗浮山红脚艾和汤阴北艾中提取精油,采用气相色谱-质谱法（GC-MS）检测精油化学成分,并测定其抗菌活性。结果表明,SD精油收率比SFE-MD高1.3~2.6倍。红脚艾精油主成分为β-石竹烯、龙脑、氧化石竹烯和大根香叶烯D等;北艾精油主成分为桉油精、松油烯-4-醇和十氢二甲基甲乙烯基萘酚等;北艾精油以单萜类物质为主,而红脚艾精油则含有更丰富的倍半萜类化合物;SFE-MD会导致部分单萜类物质的损失,如桉油精。艾叶精油对大肠杆菌、金黄色葡萄球菌、白色链珠菌有抑制及杀灭效果,且北艾精油＞红脚艾精油。     

不同产地奇异南星挥发油GC-MS分析及抗肿瘤活性

采用同时蒸馏萃取法(SDE)提取两种不同产地的奇异南星挥发油,通过气相色谱-质谱联用仪鉴定其中化学成分,采用峰面积归一化法确定各组分的相对含量,并采用甲基噻唑蓝(MTT)法对两种挥发油进行体外抗肿瘤活性测试.结果显示,从广西产和四川产奇异南星挥发油中分别鉴定出12种和11种化合物,其相对含量分别为90.96％和94.6...     

Chemical Composition,Antitumor Properties,and Mechanism of the Essential Oil from Plagiomnium acutum T. Kop.

Plagiomnium acutum T. Kop. (P. acutum) has been used as a traditional Chinese medicine for thousands of years to treat cancer but lacks evidence. The objective of this work was to reveal the chemical composition of P. acutum essential oil (PEO) and explore its potential antitumor activity and molecular mechanism. PEO was prepared by the simultaneous distillation–extraction method and characterized by gas chromatography/mass spectroscopy. CCK8 assay, flow cytometry, western blot, and immunofluorescence techniques were used to analyze the effects and mechanism of PEO against cancer cells. A total of 74 constituents of PEO were identified, with diterpenes (26.5%), sesquiterpenes (23.89%), and alcohols (21.81%) being the major constituents. Two terpenoids, selina-6-en-4-ol and dolabella-3,7-dien-18-ol, were detected in PEO for the first time. PEO showed significant cell growth inhibitory activity on HepG2 and A549 cells by blocking the G1 phase and inducing apoptosis, which may be attributed to its upregulation of p21^Cip1 and p27^Kip1 proteins and interference with mitochondrial membrane potential effect. Dolabella-3,7-dien-18-ol accounts for 25.5% of PEO and is one of the main active components of PEO, with IC₅₀ values in HepG2 and A549 cells of (25.820 ± 0.216) µg/mL and (23.597 ± 1.207) μg/mL, respectively. These results confirmed the antitumor medicinal value of P. acutum and showed great application potential in the pharmaceutical industry.