药物偶联亲和高分子微球对牛血蛋白吸附性能研究

在制备亲和高分子乳液微球基础上,对其进行空间臂改性和药物分子偶联制得PSGN-MTA亲和高分子微球药物。为研究亲和高分子微球生物相容性,考察了高分子微球药物在缓冲液中药物释放行为及对BSA蛋白吸附能力。结果表明,亲和微球表面化学键合MTA药物浓度较高且分布均匀时,对BSA蛋白的吸附过程较符合一级动力学方程。还考察了pH值对BSA蛋白吸附的影响,中性条件时PSGN-MTA微球对于BSA吸附效果较好。     

羧基磁性高分子微球的制备和表征

用改进的悬浮聚合法制备了表面含有羧基功能团的聚苯乙烯磁性微球。考察了磁微球的形态与结构 ,测定了磁微球的粒径与磁响应性 ,主要研究了单体 /水、丙烯酸 /单体、反应温度和反应时间对磁性微球形成的影响 ,并对磁性微球的生物吸附活性进行了表征。优化得到了制备具有良好生物吸附活性的羧基磁性微球的最佳实验条件     

缓蚀剂负载中空SiO2微球的制备及性能EI北大核心CSCD

以乳液聚合法制备的聚苯乙烯微球为模板制备中空介孔二氧化硅微球,经扫描电子显微镜、透射电子显微镜、热重分析、小角度X射线衍射、氮气吸附–脱附等手段对制备得到的中空微球进行性能结构表征。考察了氨水和硅源前驱体用量等制备条件对中空微球的尺寸、介孔孔径、球壳厚度等的影响,6.25 mL氨水和10 g正硅酸乙酯的用量为最佳制备条件。中空SiO2微球作为载体,负载缓蚀剂苯并三氮唑,并对负载缓蚀剂的微球表面进行高分子双电层的修饰,释放曲线实验表明,高分子双电层修饰使微球中缓蚀剂释放速率降低5倍。     

苯乙烯-对氯甲基苯乙烯共聚物胶乳微球的制备及表征

以苯乙烯（St）和对氯甲基苯乙烯（VBC）为共聚单体,过硫酸钾（KPS）为引发剂,十二烷基苯磺酸钠（SDBS）为乳化剂,采用乳液聚合法制备了富含氯甲基的苯乙烯-对氯甲基苯乙烯共聚物功能微球。采用红外光谱（FT-IR）、扫描电镜（SEM）、激光粒度仪和X射线光电子能谱（XPS）对样品进行表征,研究了乳化剂用量、单体用量、引发剂用量、反应时间、反应温度等因素对微球粒径、乳液转化率的影响。结果表明,产物微球粒径均一,表面光滑、富含氯甲基功能基团,采用此方法可以制备粒径在100～250 nm的功能高分子微球。     

缓蚀剂负载中空SiO_2微球的制备及性能

以乳液聚合法制备的聚苯乙烯微球为模板制备中空介孔二氧化硅微球,经扫描电子显微镜、透射电子显微镜、热重分析、小角度X射线衍射、氮气吸附–脱附等手段对制备得到的中空微球进行性能结构表征。考察了氨水和硅源前驱体用量等制备条件对中空微球的尺寸、介孔孔径、球壳厚度等的影响,6.25 mL氨水和10 g正硅酸乙酯的用量为最佳制备条件。中空SiO_2微球作为载体,负载缓蚀剂苯并三氮唑,并对负载缓蚀剂的微球表面进行高分子双电层的修饰,释放曲线实验表明,高分子双电层修饰使微球中缓蚀剂释放速率降低5倍。     

反相微乳液法制备磁性分子印迹聚合物微球

以二氯苯酚为模板分子、丙烯酰胺(AM)为功能单体、三羟甲基丙烷三丙烯酸酯(TRIM)为交联单体、Fe3O4为磁性组分,采用反相微乳液悬浮聚合法制备了磁性分子印迹聚合物微球(MMIPMs)。研究了吸附时间、温度、pH对吸附性能的影响,并考察了其重复利用性。结果表明,温度对二氯苯酚-MMIPMs吸附性能影响不大。底物溶液pH对MMIPMs的吸附性能有一定影响,吸附量随pH的升高而增大,当pH为7.0时,吸附量最大。MMIPMs具有良好的重复利用率。     

单步种子溶胀法与二步种子溶胀法制备氯霉素分子印迹聚合物微球的比较

以聚苯乙烯乳液为种球、氯霉素为模板分子、甲基丙烯酸为功能单体、乙二醇二甲基丙烯酸酯为交联剂,采用单步种子溶胀法与二步种子溶胀法制备了单分散氯霉素分子印迹聚合物微球,用光学显微镜和扫描电镜对微球的形貌进行了表征,并用静态吸附实验考察了微球的吸附识别性能,结果表明,二步种子溶胀法制备的微球分散性、吸附识别性能最佳,平均粒径...     

无皂乳液聚合法合成聚苯乙烯微球的研究

选用无皂乳液聚合法合成了聚苯乙烯微球乳液,并对无皂乳液聚合的最新研究动态及应用进行了介绍和总结。为了得到制备单分散微球的有利反应条件,本文研究了无皂乳液聚合体系中反应温度、单体用量、引发剂用量、反应时间等因素对聚苯乙烯微球的粒径及粒径分布的影响,同时通过透射电子显微镜(TEM)对聚苯乙烯微球进行了表征分析。实验结果表明:无皂乳液聚合法可以制备出大小均一、单分散性好的PS微球乳液;反应温度在80~95℃范围内时,温度升高,微球粒径减小,且粒径范围在300~500 nm之间;改变单体用量可以制备粒径大小不同的聚苯乙烯微球乳液;改变引发剂用量也是制备不同粒径微球的一种有效途径;延长反应聚合时间,主要是为了提高转化率,而对微球的聚合度基本没有影响。     

分子印迹聚合物中种子微球的合成及应用研究

采用辛醇和乳化剂(OS)作为复合分散剂,用悬浮聚合法制备了4-氨基吡啶分子印迹聚合物微球。采用无皂乳液聚合法对分子印迹聚合物种子微球的制备与性能进行了研究。结果表明,辛醇与极少数乳化剂复合使用,提高了乳液的稳定性,改善了种子微球粒径大小及粒径分布。当辛醇用量为单体质量的3.0%、丙烯酸乙醋与苯乙烯质量比为1:0.229、反应温度为85℃、pH值为8.0时,能够制备出期盼的分子印迹聚合物微球。     

壳聚糖表面胰蛋白酶分子印迹聚合物的制备及性能的研究

在壳聚糖表面通过希夫碱反应嫁接一层戊二醛,形成核-壳结构微球。然后在这个核-壳微球上,以胰蛋白酶为模板分子,3-氨基苯硼酸为功能单体,制备了胰蛋白酶分子印迹聚合物,通过静态吸附法,研究了聚合物的吸附性能。结果表明,印迹聚合物对模板分子有较高吸附容量和特异选择性。为从蛋白质混和溶液中分离富集胰蛋白酶提供了新的材料和方法。     

Hydrophilization of gigaporous polystyrene microspheres with saccharide as high-speed protein chromatography base support

Gigaporous poly(styrene–divinylbenzene) (PS) microspheres were hydrophilically modified with natural saccharide to minimize their nonspecific adsorption to proteins. The microspheres were chloroacetylated through Friedel–Crafts acetylation with chloroacetyl chloride, and then coupled with diacetone-D-glucose (DAGlu) through the Williamson reaction, and the protecting groups were removed on DAGlu. Results showed that the PS microspheres were successfully coupled with DAGlu and that the gigaporous structure was well maintained. After hydrophilization (Glu-PS), nonspecific adsorption of proteins on PS microspheres was greatly reduced. The high surface density of hydroxyl groups on Glu-PS microspheres surface make it easy to derivatize the spheres by classical methods. Flow experiments showed that the Glu-PS column had low backpressure, good permeability, and mechanical stability. All results indicate that the Glu-PS microspheres have great potential applications in high-speed protein chromatography.     

多孔聚(甲基丙烯酸环氧丙酯-二乙烯基苯)微球的改性及作为蛋白质疏水层析介质的应用

用悬浮聚合方法合成了富含环氧基团的多孔聚(甲基丙烯酸环氧丙酯-二乙烯基苯) [Poly(Glycidyl Methacrylate-Divinylbenzene), P(GMA-DVB)]微球,为了消除其对蛋白质的不可逆吸附,探索用聚乙二醇(Polyethylene Glycol, PEG)对微球进行改性,制备了带有PEG配基的P(GMA-DVB)微球. 实验考察了反应条件对PEG固载量的影响规律,发现最适反应温度为80℃. 以牛血清白蛋白(Bovine Serum Albumin, BSA)和胰蛋白酶作为模型蛋白,考察了PEG改性前后P(GMA-DVB)微球对蛋白质的吸附性能. 改性前P(GMA-DVB)微球有30%~40%的不可逆吸附,蛋白质回收率仅为60%~70%. 改性后介质消除了不可逆吸附,对蛋白质的吸附作用表现为可逆的疏水相互作用,吸附BSA和胰蛋白酶的质量回收率和活性回收率都在97%以上. 结果表明,PEG-P(GMA-DVB)微球可以作为一种新型介质进一步应用于蛋白质的吸附与分离.     

磁性聚合物微球在Cr(VI)吸附分离中的应用(英文)

The magnetic poly-(methacrylate-divinyl benzene) microspheres with micron size were synthesized by modified suspension polymerization method.Adsorption of Cr(VI) from aqueous solution by magnetic poly-(MA-DVB) microspheres with surface amination was investigated.The adsorption processes were carried out under diversified conditions of pH value,adsorption time and temperature to evaluate the performance of the mag-netic microspheres.The optimum pH value for Cr(VI) adsorption was found as 3.The adsorption capacity increased with adsorption time and attained an optimum at 60 min.The adsorption processes for magnetic microspheres was endothermic reaction,and the adsorption capacity increased with increasing temperature.     

Protein‐imprinted polyurethane‐grafted calcium alginate hydrogel microspheres

Protein‐imprinted polyurethane‐grafted calcium alginate hydrogel microspheres were prepared and characterized. The samples were investigated with optical microscopy, scanning electron microscopy, ¹³C‐NMR, and Fourier transform infrared spectroscopy. We proved that polyurethane side chains were successfully grafted, and this led to a relatively rough and dense surface. The samples exhibited better swelling durability when applied in specific adsorption tests. The adsorption kinetic and recognition properties indicated that the imprinted modified microspheres had excellent rebinding affinity toward the target proteins. Moreover, the influence of the preassembly pH, rebinding pH, and grafting ratio on the adsorption capacity and imprinting efficiency (IE) were systematically investigated. The study results suggest that the modified samples possessed a higher IE toward the target protein under the optimum pH and grafting ratio. Upon polyurethane grafting modification, the alginate hydrogel microspheres showed improved mechanical stability and recognition specificity. © 2015 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2015 , 132, 42140.     

胰蛋白酶分子表面印迹材料的制备及其大分子识别特性

以交联聚乙烯醇(CPVA)微球为基质,采用接枝聚合和表面印迹同步技术制备碱性蛋白胰蛋白酶(TRY)分子表面印迹材料,甲基丙烯酰氯与CPVA微球表面的羟基发生快速酯化反应,得到表面含大量可聚合双键甲基丙烯酰基(MAO)的改性微球MAO-CPVA. 按一定摩尔比将TRY和单体阴离子单体对苯乙烯磺酸钠(SSS)溶解在水溶液中,加入交联剂N,N'-亚甲基双丙烯酰胺(MBA), MAO-CPVA分散于水介质中,过硫酸铵/亚硫酸氢钠引发体系产生自由基,使包围在TRY周围的单体SSS与MBA在MAO-CPVA表面发生接枝交联聚合,制得TRY表面印迹微球MIP-PSSS/CPVA,对其进行表征,考察了其大分子识别性能. 结果表明,MIP-PSSS/CPVA对TRY有优良的亲和性和特异识别选择性,吸附容量达85.9 mg/g,对TRY的选择性系数相对于蛋白溶菌酶LZM达17.52.     

改性果胶-Fe3O4磁性微球制备及对Pb 2+吸附性能

合成了一种琥珀酸酐改性果胶-四氧化三铁（Fe₃O₄）磁性微球吸附剂,分别采用扫描电镜（SEM）、红外光谱（FT-IR）、X射线衍射（XRD）等手段对样品进行了表征,并研究了其吸附铅离子（Pb ²⁺）的性能。研究结果表明：成功制备了琥珀酸酐改性果胶-Fe₃O₄磁性微球,改性果胶包覆四氧化三铁几乎没有改变Fe₃O₄的尖晶石结构,其表面疏松多孔;改性果胶-Fe₃O₄磁性微球对铅离子的吸附符合准二级动力学方程、Langmuir等温吸附方程,吸附过程主要为化学吸附。最佳吸附条件：吸附时间为600 min,吸附温度为40 ℃,溶液pH为5,吸附剂添加量为20 mg,溶液中Pb ²⁺质量浓度为800 mg/L。改性果胶-Fe₃O₄磁性微球吸附剂用于脱除毛蚶子、扇贝酶解液中的Pb ²⁺,Pb ²⁺去除率分别为76.47%和80.34%,效果良好。     

Improving blood compatibility of natural rubber by UV‐induced graft polymerization of hydrophilic monomers

Natural rubber (NR) latex films surface‐grafted with hydrophilic monomers, poly(ethylene glycol) methacrylate (PEGMA), N‐vinylpyrrolidone (VPy), and 2‐methacryloyloxyethyl phosphorylcholine (MPC), were prepared by UV‐induced graft polymerization using benzophenone as a photosensitizer. The grafting yield increases of vulcanized NR latex films as a function of time and monomer concentration were of lesser magnitude than those of the unvulcanized NR latex films. This can be explained as a result of the crosslinked network generated during vulcanization acting as a barrier to the permeation of the photosensitizer and the monomer. The appearance of a characteristic carbonyl stretching in the attenuated total reflectance‐Fourier transform infrared spectroscopy (ATR‐FTIR) spectra of NR latex films after the surface grafting of PEGMA and MPC indicates that the modification has proceeded at least to the sampling depth of ATR‐FTIR (∼ 1–2 μm). According to the water contact angle of the modified NR latex films, the surface grafting density became higher as the grafting time and monomer concentration increased. The complete absence of plasma protein adsorption and platelet adhesion on the surface‐modified NR latex films having grafting yield above 1 wt % is a strong indication of improved blood compatibility. Results from tensile tests suggest that graft polymerization does not cause adverse effects on the mechanical properties of vulcanized NR latex films. © 2008 Wiley Periodicals, Inc. J Appl Polym Sci, 2009     

氨基功能化聚苯乙烯纳米微球的制备及其CO2吸附性能研究

以苯乙烯(St)和甲基丙烯酸缩水甘油酯(GMA)为共聚单体,通过细乳液聚合法制备出聚苯乙烯纳米微球,并研究了单体配比与反应条件对纳米微球粒径大小与分布的影响。然后利用GMA上的活性环氧基团将有机胺接枝到纳米微球表面,并将其应用于CO2吸附。采用动态激光散射、扫描电子显微镜、红外光谱、元素分析等方法对纳米微球进行了结构表征。结果表明,所制备的纳米微球最小粒径为67 nm,粒径大小均一可控,而且纳米微球表面成功地接枝上有机胺;乙二胺改性纳米微球的CO2吸附量为2.45 mmol/g,并且吸附循环稳定性较好。     

磁性复合微球处理水中氯仿的应用研究

采用磁性复合微球为吸附载体,将其应用于水体中氯仿的吸附研究,在此过程中以紫外吸光光度法就磁性复合微球粒径、表面性质对吸附性能的影响作了考察,最终选取粒径为1.5μm、表面富含胺基磁性的复合微球为最佳吸附材料。与此同时,利用CODCr法作为评价手段对磁性的复合微球吸附氯仿的处理工艺条件进行了优化,确定每处理质量浓度为74 mg/L的氯仿溶液2 mL需用磁性复合微球约1 mg,最佳吸附时间为4.0 h,磁性复合微球的富集时间为4.0 h,此时对氯仿的吸附效率大于83%。