Original article: Thermal dynamic properties of isoflavones during dry heating

Thermal stabilities of major soya isoflavones at different dry‐heating temperatures were determined in this study. The conversion of glucoside isoflavones to aglycone isoflavones was monitored as well. The thermal degradation of the isoflavones: glucoside form daidzin, glycitin, and genistin and aglycone form daidzein, glycitein, and genistein followed first‐order reaction kinetics at heating temperatures 100, 150, and 200 °C. The degradation rate constants of the six isoflavones were not significantly different at 100 °C. However, the constants increased with increasing heating temperature. The half‐life for the glucoside and aglycone isoflavones was from 144 to 169 min and 139 to 176 min at 100 °C, respectively. They decreased rapidly to 15.7–54.7 and 36.0–90.7 min when temperature increased to 150 °C. When heated at 200 °C, they further decreased to 5.8–6.0 and 15.7–21.2 min, respectively. The order of thermal stability from lowest to highest was glycitin < genistin < daidzin < glycitein < genistein < daidzein at temperature below 150 °C. However, their thermal stabilities were not different at 200 °C. The conjugated glucosides were cleaved from the isoflavones to produce their corresponding aglycones when heated at 150 °C or higher. The production of glycitein increased constantly and was the highest among the three aglycone isoflavones.     

Kinetic modelling of drying and conversion/degradation of isoflavones during infrared drying of soybean

Soy isoflavones, which are well-known for their health benefits, could be affected by the technique and condition that are used to dry soybean prior to its use. In this study the drying kinetics of soybean as well as the inter-conversion and degradation of soy isoflavones during gas-fired infrared combined with hot air vibrating drying (GFIR–HAVD) at various temperatures (50, 70, 130 and 150 °C) were investigated. Simple kinetic models for the evolutions of both moisture content and isoflavones were proposed and tested. The temperature dependency of the effective moisture diffusivity of soybean could be well described by the Arrhenius correlation with the activation energy of 13.88 kJ/mol. In terms of the evolutions of isoflavones the contents of 6″-O-malonylgenistin (MGI) and 6″-O-acetylgenistin (AGI) decreased, while genistin (GI) and genistein increased with an increase in the drying temperature and drying time. First-order kinetic models could be used to describe the changes of isoflavones. At all drying temperatures the conversion of MGI to GI exhibited the highest rate constant; this was followed by the conversion of AGI to GI. In addition, MGI had the highest degradation rate constant; this was followed, in descending order, by AGI, GI and GE. The activation energies of degradation were lower than those of inter-conversion among all forms of isoflavones.     

Moist and dry heating-induced changes in protein molecular structure,protein subfractions,and nutrient profiles in camelina seeds

The objectives of the present study were to investigate the nutritive value of camelina seeds (Camelina sativa L. Crantz) in ruminant nutrition and to use molecular spectroscopy as a novel technique to quantify the heat-induced changes in protein molecular structures in relation to protein digestive behavior in the rumen and intestine of dairy cattle. In this study, camelina seeds were used as a model for feed protein. The seeds were kept as raw (control) or heated in an autoclave (moist heating) or in an air-draft oven (dry heating) at 120°C for 60 min. The parameters evaluated were (1) chemical profiles, (2) Cornell Net Protein and Carbohydrate System protein subfractions, (3) nutrient digestibilities and estimated energy values, (4) in situ rumen degradation and intestinal digestibility, and (5) protein molecular structures. Compared with raw seeds, moist heating markedly decreased (52.73 to 20.41%) the content of soluble protein and increased (2.00 to 9.01%) the content of neutral detergent insoluble protein in total crude protein (CP). Subsequently, the rapidly degradable Cornell Net Protein and Carbohydrate System CP fraction markedly decreased (45.06 to 16.69% CP), with a concomitant increase in the intermediately degradable (45.28 to 74.02% CP) and slowly degradable (1.13 to 8.02% CP) fractions, demonstrating a decrease in overall protein degradability in the rumen. The in situ rumen incubation study revealed that moist heating decreased (75.45 to 57.92%) rumen-degradable protein and increased (43.90 to 82.95%) intestinal digestibility of rumen-undegradable protein. The molecular spectroscopy study revealed that moist heating increased the amide I-to-amide II ratio and decreased α-helix and α-helix-to-β-sheet ratio. In contrast, dry heating did not significantly change CP solubility, rumen degradability, intestinal digestibility, and protein molecular structures compared with the raw seeds. Our results indicated that, compared with dry heating, moist heating markedly changed protein chemical profiles, protein subfractions, rumen protein degradability, and intestinal digestibility, which were associated with changes in protein molecular structures (amide I-to-amid II ratio and α-helix-to-β-sheet ratio). Moist heating improved the nutritive value and utilization of protein in camelina seeds compared with dry heating.     

Heat-induced conversion of gingerols to shogaols in ginger as affected by heat type (dry or moist heat), sample type (fresh or dried), temperature and time

The impact of heat type, sample type, temperature and time on the heat-induced conversion of gingerols to shogaols in ginger were studied by an UHPLC–ESI–MS/MS. Heat treatments greatly induced the conversion of gingerols to shogaols in ginger. As the temperature increased, the faster conversion of gingerols into shogaols were observed. However, the efficiency of the heat-induced conversion differed greatly with the heat types. Moist heat treatment induced significantly higher quantity of shogaols than dry heat treatment. The moist heat treatment at 120 °C for 360 min induced the highest conversion, reaching to 2991 mg 6-shogaol per kg ginger. In addition, dry-heat induced conversion was affected by the sample type. The dry-heat treatment on dried powder induced significantly higher quantity of shogaols than that on sliced fresh ginger. This represents the first systematic comparative study on the heat and sample types on the heat-induced conversion of gingerols into shogaols in ginger.     

Dry and moist heating-induced changes in protein molecular structure, protein subfraction, and nutrient profiles in soybeans

Heat processing has been used to improve protein utilization and availability of animal nutrition. However, to date, few studies exist on heat-induced protein molecular structure changes on a molecular basis. The aims of this study were to use molecular spectroscopy as a novel approach to determine heat-induced protein molecular structure changes affected by moist and dry heating and quantify protein molecular structures and nutritive value in the rumen and intestine in dairy cattle. In this study, soybean was used as a model for feed protein and was autoclaved at 120°C for 1 h (moist heating) and dry heated at 120°C for 1 h. The parameters assessed in this study included protein structure α-helix and β-sheet and their ratio, protein subfractions associated with protein degradation behaviors, intestinal protein availability, and energy values. The results show that heat treatments changed the protein molecular structure. Both dry and moist heating increased the amide I-to-amide II ratio. However, for the protein α-helix-to-β-sheet ratio, moist heating decreased but dry heating increased the ratio. Compared with dry heating, moist heating dramatically changed the chemical and nutrient profiles of soybean seed. It greatly decreased soluble crude protein, nonprotein nitrogen, and increased neutral detergent insoluble protein. Both dry and moist heating treatments did not alter digestible nutrients and energy values. Heating tended to decrease the nonprotein nitrogen fraction (soluble and rapidly degradable protein fraction) and true protein 1 fraction (fast-degradable protein fraction). Conversely, the true protein 3 fraction (slowly degradable fraction) significantly increased. The in situ rumen study showed that moist heating decreased protein rumen degradability and increased intestinal digestibility of rumen-undegradable protein. Compared with the raw soybeans, dry heating did not affect rumen degradability and intestinal digestibility. In conclusion, compared with dry heating, moist heating dramatically affected the nutrient profile, protein subfractions, rumen degradability, intestinal digestibility, and protein molecular structure (amide I-to-II ratio; α-helix-to-β-sheet ratio). The sensitivity of soybean seed to moist heating was much higher than that to dry heating in terms of the structure and nutrient profile changes.     

发酵处理对豆豉抗氧化活性影响的研究

发酵处理基本上不改变豆豉中异黄酮的总含量,而糖苷型大豆异黄酮在β-葡萄糖苷酶的作用下部分转化为游离型大豆异黄酮,使游离型大豆异黄酮的含量明显提高;在样品未添加任何抗氧化剂的前提下,通过试验发现豆豉的自身抗氧化性比原料熟黑豆强许多。     

发酵处理对大豆制品中异黄酮含量与组分的影响

利用发酵大豆食品———豆豉与酸豆乳作为试验对象 ,通过与对照品比较 ,发现发酵处理对大豆食品中的异黄酮总含量的影响不大 ,但对其异黄酮的组分有较大的影响 ,发酵处理使糖苷型大豆异黄酮在 β 葡萄糖苷酶的作用下水解为游离型大豆异黄酮 ,从而使发酵制品中的游离型大豆异黄酮的含量增高 ,而糖苷型大豆异黄酮的含量降低     

弱碱水解丙二酰基型大豆异黄酮的研究

本实验采用碱法将丙二酰基大豆异黄酮水解为葡萄糖苷型大豆异黄酮。通过正交实验得到最佳碱水解条件为:PH=11.0,水解温度65℃,水解时间1h。水解前样品中大豆异黄酮的含量分别是:D:16.12%,G:14.49%,MD:33.62%,MG:35.77%,水解后样品中大豆异黄酮的含量分别是:D:46.86%,G:47.34%,MG:5.80%,MD:nd。     

Physicochemical characteristics and anti-oxidant activities of farm-cultivated and mountain-cultivated ginseng seeds

Physicochemical characteristics and anti-oxidant capacities of seeds from two farm-cultivated and one mountain-cultivated ginsengs (Panax ginseng Meyer) (MG) were determined. The seeds had 17.9–22.1% (dry basis) crude lipids, 11.5–15.2% crude proteins, and 1.4–1.7% ash. Oleic acid (77.9–78.5%) was the predominant fatty acid in the seed oils, followed by linoleic acid (16.6–17.4%). The highest Hunter b value, carotenoids, (β?+?γ)-tocotrienol, and δ-tocotrienol, and the lowest α-tocotrienol were observed in the seed oils from MG. Squalene was also the most abundant in the MG seed oils. β-Sitosterol was the major phytosterol in the seed oils with MG the highest. Defatted seed meal extracts from MG possessed the most total phenolics and flavonoids, and the highest DPPH and ABTS radical scavenging activities. These results suggest that MG seeds may be a novel source of functional foods.     

Quantitative analysis of the isoflavone content and biological growth of soybean (Glycine max L.) at elevated temperature,CO2 level and N application

Effects of ambient and elevated CO₂ levels (360 and 650 µmol mol^?1 respectively), ambient and high (5 °C above ambient) temperatures and their interactions with N application on soybean (Glycine max L.) were studied in 2001. Overall, total isoflavones in whole soybean seeds were highest (1383.0 µg g^?1) in the elevated CO₂ (AE) treatment without N application and lowest (414.1 µg g^?1) in the elevated temperature (EA) treatment with N application. Malonylgenistin (449.2 µg g^?1) and malonyldaidzin (435.9 µg g^?1) concentrations in the AE treatment without N application were highest among the 12 individual isoflavones, while aglycon and acetyl conjugates showed lower concentrations (below 10 µg g^?1) than glucoside and malonyl conjugates in all treatments. Overall, N application had no effect on total isoflavone concentration, while both temperature and CO₂ level had a higher effect on increasing isoflavones, including aglycon and acetyl conjugates (P = 0.001). In the biological growth analysis, total dry weight was highest (100.9 g) in the elevated temperature and CO₂ (EE) treatment with N application, while leaf area was more affected by CO₂ than by temperature and increased with N application. There were larger numbers of pods (99) and seeds (176) per plant in the EE treatment with N application, and generally the AE treatment showed a greater increase in 100‐seed weight (g per 100 seeds) and in pods and seeds per plant than other treatments. Overall, total dry weight was highly affected (P = 0.001) by three main factors, temperature, CO₂ and N application, but the interactions temperature × N and temperature × CO₂× N did not affect total dry weight. Also, total dry weight tended to increase with increasing numbers of pods (r² = 0.93***) and seeds (r² = 0.93***) and larger leaf area (r² = 0.85***). In addition, numbers of pods and seeds were significantly affected (P = 0.01–0.001) by temperature, CO₂ and temperature × CO₂. Generally, elevated CO₂ and temperature did not affect N, P and K concentrations in the seeds but did decrease the concentrations of Ca and Mg, which were increased in the AE treatment. Among the nutrients, Ca and Mg were highly correlated with temperature and CO₂ level. N concentration in the seeds increased with applied N and in particular showed a high increase with elevated temperature and ambient CO₂ (EA treatment). The variation in isoflavones was correlated with temperature (r² = ?0.70**) and CO₂ (r² = 0.67**), while N application was not correlated with isoflavone concentration. Also, Ca (r² = ?0.85***) and Mg (r² = ?0.57*) in the seeds were correlated with variation in isoflavones. This indicated that isoflavones were in higher concentrations under conditions of low temperature and increasing CO₂, which also resulted in low Ca and Mg concentrations in the seeds. The results of this study suggest that the long‐term adaptation of the soybean to growth at elevated CO₂ level and high temperature might potentially increase its isoflavone content, growth and yield. Copyright © 2005 Society of Chemical Industry     

Sterols heating: degradation and formation of their ring-structure polar oxidation products

Cholesterol and phytosterols can be oxidised under heating conditions to give sterol oxidation products (SOPs), known by their toxic effects. This paper studied the degradation of cholesterol and three plant sterols during a 360 min heating treatment (180 °C). The formation and further degradation of SOPs was also analysed by GC-MS. Results revealed a sterol susceptibility to degradation according to the following decreasing order: campesterol≈β-sitosterol≥stigmasterol>cholesterol. The degradation curve fit (R(2)=0.907-0.979) a logarithmic model. SOPs increased their concentration during the first 5-10 min and thereafter, their degradation rate was higher than their formation rate, resulting in a decrease over time. Irrespective of the sterol from which they had derived, 7-keto derivatives presented the highest levels throughout the entire process, and also SOPs with the same type of oxidation followed a similar degradation pattern (R=0.90-0.99).     

Val bean (Lablab purpureus L.) proteins: composition and biochemical properties

Val bean (Lablab purpureus L.) proteins were fractionated using the Osborne protein fractionation scheme and biochemically characterized. The seed flour contained 302 g kg^?1 protein (micro‐Kjeldahl N × 6.25) on a dry weight basis. Albumin, globulin, prolamin, and glutelin accounted for 22.8%, 45.1%, 1.8% and 30.3%, respectively, of the total soluble seed proteins. Among the solvents tested, 0.1 mol L^?1 aqueous NaOH was the most effective protein solubilizer. Isoelectric focusing indicated the seed proteins to be predominantly acidic (pI range was ～4–7). Val globulin is a glycoprotein composed of at least three polypeptides in the molecular mass range 51–64 kDa. Albumin fraction had the highest trypsin inhibitory activity, while the globulin fraction registered the highest hemagglutinating activity. Sulfur amino acids were the first limiting amino acids in the total seed proteins. The proportion of essential to total [E/T(%)] amino acids for the bean flour was 36.97%. Among the protein fractions, glutelin fraction had the highest E/T (42.86%) followed by albumin (41.57%), globulin (39.87%), and prolamin (39.15%). Native globulin, although resistant to pepsin, was effectively digested in vitro upon moist heat (100 °C, 30 min) denaturation. Copyright © 2007 Society of Chemical Industry     

Structural consequences of dry heating on Beta-Lactoglobulin under controlled pH

Heating in dry state has gained a lot of interest in pharmaceutical and food industries for viral and microbial decontamination of thermo sensible products. Controlled dry heating has now become a common industrial process for improving the functional properties of food proteins. Besides this improvement, chemical modifications in protein structures involving degradation of amino acids, new intra or inter-molecular disulfide bonds, isopeptide bonds, and some other links may occur. These chemical modifications are favored by severe heating under neutral or alkaline conditions, and except disulfide interchanges, are usually not predominant during heat treatments in solution and their occurrence is not well understood. Understanding chemical modifications in protein structure that occur during dry heating of protein powders is a prerequisite for reproducible properties of final products at industrial scale. In the present work, we focused on how dry heating under acidic pH conditions affects the chemical modifications and denaturation/aggregation reactions of whey proteins. Model whey protein (β-lactoglobulin) powders obtained from freeze drying of protein concentrates adjusted to pHs 2.5 & 6.5 were adjusted to a fixed water activity (Aw 0.23). The samples were dry heated at 100 °C for up to 24 hours and structural modifications induced during dry heating were followed. We showed that whatever the pH value, proteins were characterized by irreversible mass losses of 18 Da. Such mass losses were increased at lower pH value. Dry heating mainly generated small aggregates (dimers and oligomers). Strikingly, at pH 2.5 intermolecular disulfide bonds were the only crosslinks between proteins in the aggregated forms while covalent crosslinks other than disulfide bonds also participated at pH 6.5. β-Lactoglobulin hydrolysis was also detected at pH 2.5 and some of the peptides were incorporated in the oligomers. These results underline that chemical modifications in protein structures induced by dry heating are highly pH dependent. Hence, strict control of pH conditions for dry heating is indispensable to give reproducible functionality in products where such modified proteins are incorporated as ingredient.     

EFFECT OF POSTHARVEST HOT AIR AND FUNGICIDE TREATMENTS ON THE QUALITY OF 'MARADOL' PAPAYA (CARICA PAPAYA L.)

Papaya (Carica papaya L.) fruit is very perishable with a maximum postharvest life of 4 weeks. The effect of forced moist (100% RH) or dry (50%) hot air with or without Thiabendazole (TBZ at 1.2 g active ingredient/L) on the chilling injury (CI), fungi development and quality of ‘Maradol’papaya fruit stored at 5C or 20C for up to 42 days was investigated. Moist hot air at 48.5C or 50C for 4 h caused fruit injury. Dry air at 48.5C for 4 h, alone or in combination with TBZ, decreased CI intensity and fungi development, and the best effect was achieved by combining dry heat treatment and TBZ, without causing negative effects on fruit quality and without causing heat injury. Hot air treatment, especially in combination with TBZ, decreased growth of inoculated Colletotrichum gloeosporioides. Trehalose was not detected in papaya, probably due to the presence of trehalase. Hot water treatment, with or without TBZ, did not cause any negative effect on the content of β-carotene and lycopene.     

Correlation of methylglyoxal with acrylamide formation in fructose/asparagine Maillard reaction model system

α-Dicarbonyl compounds were highly reactive intermediates formed in Maillard reaction (MR), and o-phenylenediamine (OPD) was widely used as a trapping agent for α-dicarbonyl compounds. Both aqueous fructose/asparagine (Fru/Asn) and fructose/asparagine/o-phenylenediamine (Fru/Asn/OPD) model systems were heated at 150 °C for up to 30 min. Methylglyoxal (MG) was the main α-dicarbonyl compounds formed in MR, which was chosen as a representative of α-dicarbonyl compound to investigate the influence on acrylamide (AA) formation. The concentrations of AA, MG and Asn were detected during MR by HPLC method. The results indicated that the formation of AA increased with the heating time, and nearly 75% of AA was formed through the participation of α-dicarbonyl compounds. The amounts of formation and consumption of MG increased with heating time, and from 12 min of reaction, the consumed amounts of MG accounted for 62.1–90.3% on the basis of total amounts of MG formed in MR, suggesting that most of the MG took part in further reactions. Meanwhile, Asn concentration decreased with heating time in both models. The formation of AA and consumption of Asn were highly correlated with MG. Indeed, as MG concentration in MG/Asn model system decreased during heating at 150 °C, the concentration of AA significantly increased. The coefficient of correlation between consumed amounts of MG and the formed amounts of AA was 0.931, demonstrating that MG plays a role in AA formation.     

Modeling thermal degradation of litchi texture: Comparison of WeLL model and conventional methods

The pasteurization process of litchi causes thermal texture degradation. In order to optimize the process condition, the thermal firmness degradation kinetics of litchi was studied. Linear regression of first order kinetics-Arrhenius equation and non-linear regression of Weibull-Log logistic model (WeLL) were employed. Fractional conversion technique was also used to examine its effect on improving the accuracy of models. The results indicated that in three thermal treatments at 80, 85 and 90 °C, the thermal firmness degradation curve of litchi showed a short term shoulder-like increase at the initial heating stage, followed by a decrease and remained a tail-like non-zero equilibrium stage during prolonged heating. WeLL model displayed better accuracy for litchi texture modeling than first order-Arrhenius equation. Fractional conversion technique was effective in increasing the accuracy of first order kinetics comparable to those of Weibull model. It is concluded that WeLL model can be applied in modeling thermal food texture degradation.     

Moist and Dry Heat Reduce Isopropyl N-(3-Chlorophenyl) Carbamate (CIPC) Residue in Potatoes

Moist (boiling and pressure-cooking) and dry heat (baking with and without foil, and microwaving) were compared as to their effect on CIPC retention in potatoes. Tubers were dipped in a 1% emulsion of CIPC for 5 min. prior to processing and stored at 5°C for 0, 1 and 2 mo. All processing methods significantly (p<0.01) reduced CIPC residue in potatoes. Moist heat methods reduced CIPC more than dry heat. Boiling resulted in significantly (p<0.01) greater reduction of CIPC than pressure-cooking while baking without foil resulted in greatest reduction of CIPC followed by microwaving and baking with foil. The peel localized 20 times more CIPC residue than the cortex.     

Effect of radiation and other processing methods on protein quality of sunflower meal

The effects of gamma irradiation from a ⁶⁰Co source (10 and 20 kGy), dry heating (121 °C for 10, 20 and 30 min), autoclaving (121 °C at 103.5 kPa for 10, 20 and 30 min) and their combination on chlorogenic acid, soluble protein, available lysine and in-vitro protein digestibility of sunflower meal were studied. The moisture content of the raw sample was 78 g kg⁻¹ as is and on a dry matter basis. The meal contained 26.7 g kg⁻¹ chlorogenic acid, 330 g kg⁻¹ crude protein, 78.5% soluble protein and 2.63 g 16 g N⁻¹ available lysine. Digestibility of raw meal was 81.5%. Chlorogenic acid, soluble protein and available lysine of raw meal decreased during dry heating, autoclaving and radiation processing. The digestibility was significantly affected by processing method (P < 0.05), as well as by the time of dry heating and autoclaving. The influence of combination methods revealed that irradiation alone had a little effect on chlorogenic acid and in-vitro protein digestibility. Autoclaving plus irradiation up to 20 kGy markedly improved the digestibility (90%). Therefore, the results suggested that the combination of autoclaving for 10 min plus irradiation up to 20 kGy has a beneficial effect on the protein quality of sunflower meal with little effect on its content of soluble protein, available lysine and markedly reduced chlorogenic acid by 87%, more than other processing methods. © 1999 Society of Chemical Industry     

Determination of isoflavone content and antioxidant activity in Psoralea corylifolia L. callus cultures

Relation between isoflavones production and antioxidant activity in Psoralea corylifolia cell cultures was studied. High performance liquid chromatography analysis revealed that root-derived callus cultures produced maximum amount of daidzein whereas genistein by leaf-derived callus. Cell cultures grown under continuous illumination (40-μmol m⁻² s⁻¹) produced several-fold more isoflavones daidzein (2.28% dry wt) and genistein (0.21% dry wt) than that of field grown plants. The antioxidant activity of extracts was determined using 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging assay, phosphomolybdenum assay and correlated with the content of total phenolics in the extracts. Calli grown under continuous illumination exhibited strong antioxidant activities compared to dark grown callus cultures and explants materials.     

Effect of dextrose equivalency of maltodextrin together with Arabic gum on properties of encapsulated beetroot juice

Maltodextrin (MD) with a dextrose equivalency of 10 and 20 (10DE and 20DE) and Arabic gum (AG) were used as encapsulating materials for beetroot juice by spray drying. The produced encapsulates were evaluated in terms of encapsulation yield, moisture content, hygroscopicity, bulk density, color parameters (L^*, a^*, b^* and TCD), radical scavenging activity (RSA), total phenolic content (TPC) and betalain content. The binary blend of encapsulating material significantly (P < 0.05) affected the studied responses. The highest bulk density (0.549 g/mL) was observed in encapsulates based on MD (20DE) as the encapsulating material. A highest encapsulating yield of 73.27 % and a redness (a^*-value) of 39.03 was referred to the formulation of MD (DE10) and AG. Formulation with MD (20DE) and MD (DE20) + AG kept better retention of functional assets (RSA and TPC) in microencapsulates produced. The increase in the value of betalain content (208.06 mg/100 g dm) was well correlated with redness (a^*-value). The studied responses ties of all encapsulate produced with formulation MD (DE10) + AG was found to be satisfactory with an exception for their lower bulk density.