未破裂异位妊娠保守治疗56例临床分析

目的探讨异位妊娠中西医结合保守治疗的疗效。方法将2005年1月至2009年1月于铁西中西医结合医院就诊的符合保守治疗条件的异位妊娠患者56例,采用甲氨喋呤联合中药治疗。结果56例中成功41例,占73.2%,15例失败改手术治疗,占26.8%。结论异位妊娠采用中西医结合治疗能保守杀胚,促进包块吸收,保留生育功能,开辟了非手术治疗途径。     

健康教育对异位妊娠患者保守治疗的临床意义

目的探讨健康教育对异位妊娠保守治疗患者的治疗意义。方法300例异位妊娠保守治疗患者随机分为观察组和对照组,对照组进行常规治疗和护理,观察组进行常规治疗和护理外,还进行心理护理、个体化指导及出院指导等健康教育。结果观察组患者在心理焦虑发生率、并发症发生率及保守治疗失败率与对照组比较,有非常显著性差异(P<0.01)。结论对异位妊娠保守治疗患者开展健康教育可改善治疗效果。     

保守疗法治疗异位妊娠的观察及护理

目的 观察异位妊娠应用药物保守治疗的临床效果,并观察护理方法,以积累经验,指导临床工作。方法 对我院2008年1月至2009年12月收治的异位妊娠患者(共96例),应用米非司酮和天花粉汤剂进行治疗。治疗后进行B超检查,并监测血液和尿液中人绒毛膜促性腺激素(β-HCG)的含量(每周2次)。患者全程进行规范护理。结果 经规范治疗及护理后,治愈89例,占92.71%,无效7例,占7.29%。结论 异位妊娠患者可以采用保守疗法,但是要注意用药的具体方法及合理监测,同时加强护理至关重要。     

甲氨蝶呤、甲酰四氢叶酸联合中药治疗异位妊娠

目的为了提高宫外孕的保守治愈率,我院用甲氨蝶呤(MTX)、甲酰四氢叶酸联合中药治疗异位妊娠。方法甲氨蝶呤1mg/kg静滴,用药1、3、5d;第2、4、6天用甲酰四氢叶酸0.1mg/(kg·次)肌注每日2次,同时服中药,治疗异位妊娠178例。结果治愈152例,治愈率85.4%。副反应轻,复查肝肾功能正常。结论使用甲氨蝶呤、甲酰四氢叶酸、中药联合治疗异位妊娠,疗效好、安全、简单、价廉、不良反应少。     

甲氨喋呤配合米非司酮保守治疗异位妊娠32例临床分析

目的探讨应用甲氨喋呤肌肉注射配合米非司酮保守治疗异位妊娠的疗效。方法选择病例32例,其中住院病人12例,门诊随诊病人20例,用药前作血常规及血的生化检查,无异常后甲氨喋呤0.4mg(kg·d),肌肉注射,1次/d,共5d,米非司酮50mg,2次/d,共6d,同时给予对症治疗,治疗期间严密观察生命体征变化以及腹痛,阴道流血情况。并定期复查血,尿HCG。根据血β-HCG及尿HCG的结果,必要时重复给药1次。结果甲氨喋呤配合米非司酮保守治疗异位妊娠的成功率达87.5%,使87.5%的病人免受了手术的痛苦。结论甲氨喋呤配合米非司酮治疗异位妊娠的成功率高,也可门诊随诊治疗。     

异位妊娠51例临床治疗分析

目的探讨异位妊娠的诊断、治疗方法、预防措施。方法分析我院近4年收治的51例异位妊娠患者的资料。结果51例经不同治疗方法,治愈48例,治愈率95.4%。5例保守治疗,3例成功,成功率60%,2例失败,血B-HCG上升,包块增大,腹痛加重,腹腔积液增多,均行输卵管切除术,失败率40%。结论对不同异位妊娠的治疗方法,对有生育要求的尽量保留其生育功能。     

127例异位妊娠保守治疗的临床分析

目的探讨适合基层医院应用的保守治疗异位妊娠的方法。方法将我校附院2004年1月至2007年5月收治的未破裂异位妊娠病例127例随机分成两组,实验组采用MTX0.4mg/kg单次注射,加用中药。对照组采用传统方法—MTX1mg/kg肌注1次单纯治疗。结果实验组的治疗效果明显优于对照组,P<0.05。结论MTX单次肌注结合中药治疗异位妊娠疗效好,副作用小,是适合在基层医院推广应用的保守治疗方法。     

甲氨蝶呤联合米非司酮治疗异位妊娠的疗效观察

目的探讨甲氨蝶呤联合米非司酮治疗异位妊娠患者的方法及疗效。方法将2008年7月至2010年7月我院保守治疗的异位妊娠患者45例中随机分为采用甲氨蝶呤联合米非司酮治疗观察组23例和采用米非司酮治疗的对照组22例,对2组的治疗效果进行比较。结果 2组治疗后的包块缩小、症状消失、尿HCG转阴、血HCG见下降、治愈率相比差异均有显著性差异(P<0.01或P<0.05)。结论甲氨蝶呤联合米非司酮治疗异位妊娠患者疗效明显,值得临床推广运用。     

米非司酮治疗异位妊娠临床观察及护理

目的探讨米非司酮治疗异位妊娠的观察及护理方法。方法对异位妊娠患者给予口服米非司酮,进行用药前准备、用药后护理、心理护理及出院指导。结果15例服米非司酮患者13例治疗成功。结论米非司酮终止早孕是一种安全、可靠、简便和有效的非手术方法,减少了手术带来的损伤,减轻了患者的痛苦。     

甲氨喋呤保守治疗异位妊娠疗效观察

目的探讨甲氨喋呤(MTX)治疗异位妊娠效果和用药方法。方法将48例患者随机分成A组和B组。A组,给予甲氨喋呤50mg肌肉注射,隔日再次给予氨甲喋呤50mg肌注,B组,仅给予甲氨喋呤50mg单次肌肉注射。结果A组有22例(84.6%)获得成功,B组有18例(81.8%)获得成功,成功率无显著差别,A组发生副反应8例(30.8%),B组发生副反应3例(13.6%),B组副反应发生率显著低于A组。结论甲氨喋呤用于异位妊娠保守治疗疗效显著,其中单次剂量肌注法更为简便,副反应小,值得临床推广应用。     

Variation in stability of endogenous reference genes in fallopian tubes and endometrium from healthy and ectopic pregnant women

RT-qPCR is commonly employed in gene expression studies in ectopic pregnancy. Most use RN18S1, β-actin or GAPDH as internal controls without validation of their suitability as reference genes. A systematic study of the suitability of endogenous reference genes for gene expression studies in ectopic pregnancy is lacking. The aims of this study were therefore to evaluate the stability of 12 reference genes and suggest those that are stable for use as internal control genes in fallopian tubes and endometrium from ectopic pregnancy and healthy non-pregnant controls. Analysis of the results showed that the genes consistently ranked in the top six by geNorm and NormFinder algorithms, were UBC, GAPDH, CYC1 and EIF4A2 (fallopian tubes) and UBC and ATP5B (endometrium). mRNA expression of NAPE-PLD as a test gene of interest varied between the groups depending on which of the 12 reference genes was used as internal controls. This study demonstrates that arbitrary selection of reference genes for normalisation in RT-qPCR studies in ectopic pregnancy without validation, risk producing inaccurate data and should therefore be discouraged.     

经阴道彩超诊断早期异位妊娠84例的临床分析

目的探讨经阴道超声对早期异位妊娠的诊断价值。方法选取我院2010年1月至2010年12月收治的经临床诊断为异位妊娠的患者为研究对象,对该组患者进行经腹超声和经阴道超声检查,比较其诊断率,总结异位妊娠的声像学特点。结果经阴道超声的确诊率为84.5%(71/84)显著高于经腹超声的67.9%(57/84),P<0.05。异位妊娠典型的声像特点为在附件区探及异常回声团块,部分可见胎囊、胎芽及心管搏动。结论经阴道彩超的分辨率较高,具有无创性和可重复性强的特点,对早期异位妊娠的诊断具有较高的价值。     

超声诊断不同类型异位妊娠120例的临床应用

目的分析超声诊断不同类型异位妊娠的临床价值。方法选择我院2007年6月至2010年6月收治的120例经超声确诊的异位妊娠患者作为研究对象,采用美国GE-LOGIQ-400、Acuson128XP/10c彩色多普勒超声诊断仪。彩色多普勒超声诊断仪进行检查,总结分析不同类型异位妊娠的超声声像图表现。结果超声诊断的120例异位妊娠患者,经手术及病理证实者112例,诊断符合率93.3%。120例超声诊断的异位妊娠患者中,未破型、破裂型、流产型、陈旧型异位妊娠患者的超声声像图表现均有其自身的特点。结论超声对异位妊娠的诊断符合率较高,不同类型的异位妊娠超声声像图具有不同的特点,因此,临床诊断异位妊娠时同时结合HCG及患者自身的因素而确诊。     

宫外孕临床诊治的探讨

目的探讨宫外孕的超声图像特点,及其在指导临床治疗措施中的应用。方法对36例宫外孕患者的阴道超声图像特点进行分析,根据超声图像特点治疗措施分为手术治疗和药物保守治疗。结果36例经阴道超声诊断为宫外孕患者,其中检出胚芽搏动者15例,以及检出最大径线<3.0cm不均质包块患者5例,临床采取手术治疗;余16例采取保守治疗。结论阴道超声检查在宫外孕早期的定位诊断中起着重要的作用,并对临床医生根据超声图像特点结合临床表现采取相应治疗措施,具有重要意义。     

Aberrant Sphingolipid Metabolism in the Human Fallopian Tube with Ectopic Pregnancy

Sphingosine 1-phosphate (S1P), a product of sphingomyelin metabolism, is generated via phosphorylation of sphingosine by sphingosine kinases (SphK). It acts via a family of G protein-coupled receptors or as an intracellular second messenger for agonists acting through the S1P receptors (S1P1–5). In our study, the expression of SphK1 and S1P1 was identified by immunohistochemistry and immunoblot. The concentration of S1P was measured using ELISA. The spontaneous contraction of isolated fallopian tube strips was determined by tension recording. Our results showed that SphK1 and S1P1 were localized in the fallopian tube epithelial cells. In addition, smooth muscle cells also contained S1P1. Compared with the intrauterine pregnancy group, SPHK1 and S1P1 were overexpressed in ectopic pregnancy. However, the S1P concentration within the human oviduct from ectopic pregnancy subjects was largely reduced than that from normal pregnancy subject. The results from tension recording indicated that exogenous and intracellularly generated S1P can regulate the spontaneous contraction of oviduct isolated from rats and human. In conclusion, the sphingolipid metabolism signal pathway functionally existed in the human fallopian tube. Aberrant sphingolipid metabolism in the human fallopian tube may be involved in ectopic pregnancy.     

化妆品标签新规倍受关注

对我国的中医药文化在化妆品中的应用进行了论述。对我国的中药化妆品市场进行了分析。对“中草药”列人化妆品标识禁语,化妆品中禁用中药的表述在业界引起了争议。建议国家有关部门更好地利用我国的中药、中草药资源,加大对药妆的扶持力度以及化妆品市场的监管力度,在保护我国中草药日化市场的同时,提高企业的美容中医理论与中草药研究的技术水平和中草药化妆品的质量。     

中药芳疗常用品种解析

中药芳疗是借助中药及香料药物的疗效作用,达到扶正祛邪、调整阴阳、调理气血、调治脏腑的功效。根据各类中药的不同功效,在辩证的基础上,选择适宜的中药品种,通过中药对全身的调理,从而达到补益强身、葆春养颜、延缓衰老以及治疗损容性疾病和纠正损容性缺陷的目的。根据中药芳疗的特点,侧重介绍外用中药的部分品种,即运用药物直接作用于体表局部,并使药物的性能直达病灶,产生治疗和保健驻颜的效果。     

The Regulation of Nitric Oxide Synthase Isoform Expression in Mouse and Human Fallopian Tubes: Potential Insights for Ectopic Pregnancy

Nitric oxide (NO) is highly unstable and has a half-life of seconds in buffer solutions. It is synthesized by NO-synthase (NOS), which has been found to exist in the following three isoforms: neuro nitric oxide synthase (nNOS), inducible nitric oxide synthase (iNOS), and endothelial nitric oxide synthase (eNOS). NOS activity is localized in the reproductive tracts of many species, although direct evidence for NOS isoforms in the Fallopian tubes of mice is still lacking. In the present study, we investigated the expression and regulation of NOS isoforms in the mouse and human Fallopian tubes during the estrous and menstrual cycles, respectively. We also measured isoform expression in humans with ectopic pregnancy and in mice treated with lipopolysaccharide (LPS). Our results confirmed the presence of different NOS isoforms in the mouse and human Fallopian tubes during different stages of the estrous and menstrual cycles and showed that iNOS expression increased in the Fallopian tubes of women with ectopic pregnancy and in LPS-treated mice. Elevated iNOS activity might influence ovulation, cilia beats, contractility, and embryo transportation in such a manner as to increase the risk of ectopic pregnancy. This study has provided morphological and molecular evidence that NOS isoforms are present and active in the human and mouse Fallopian tubes and suggests that iNOS might play an important role in both the reproductive cycle and infection-induced ectopic pregnancies.