Methyl jasmonate reduces chilling injury symptoms and enhances colour development of ‘Kent’ mangoes

Treatment of mango (Mangifera indica cv ‘Kent’) fruits with methyl jasmonate (MJ) vapour for 20 h at 20 °C reduced chilling injury (CI) symptoms and enhanced skin colour development. MJ at 10^?4 M was the most effective concentration for reducing CI and decay in fruits stored at 5 °C followed by 7 days at 20 °C (shelf‐life period). The use of 10^?5 M MJ enhanced yellow and red colour development of mangoes stored at 20 °C. These fruits possessed higher L*, a* and b* values than untreated fruits and those treated with 10^?4 M MJ. Ripening processes were inhibited by cold storage (5 °C) in control fruits. After cold storage and shelf‐life period, fruits treated with 10^?5 M MJ ripened normally and contained the highest total soluble solids (TSS). These fruits also maintained higher sugar and organic acid levels than fruits subjected to other treatments. We concluded that MJ treatment could be used to reduce decay and CI symptoms and also to improve colour development of mango fruits without adversely affecting quality. © 2001 Society of Chemical Industry     

Hot water and curing treatments reduce chilling injury and maintain post-harvest quality of 'Valencia' oranges

‘Valencia’ oranges [Citrus sinensis (L.) Osbeck] were harvested at optimal maturity and either dipped in hot water at 53 °C for 3 or 6 min or at 48 °C for 12 min or cured at 53 °C for 1 or 6 h or at 48 °C for 12 h. The fruits were not degreened, waxed or treated with any post‐harvest fungicides. All fruit samples were stored at 4 °C for 6 months following the treatments. Both hot water dip and curing treatments reduced chilling injury and decay when compared with the untreated control. The most effective treatments were curing of fruit at 53 °C for 6 h and at 48 °C for 12 h. Weight loss and juice yield were higher in cured fruits than those from other treatments, but the heat treatments had no consistent effects on titratable acid, soluble solids, ascorbic acid and peel colour. It was concluded that a pre‐storage hot water dip and curing at high temperatures might be beneficial in preventing chilling injury and decay of ‘Valencia’ oranges for 6 months of storage at 4 °C.     

Effect of hot air treatment on quality and ripening of Chinese bayberry fruit

BACKGROUND: Postharvest decay and softening are the major factors limiting the extension of storage life of Chinese bayberry fruit. To evaluate the effects of hot air treatment (HAT) on Chinese bayberry fruit, fruits were stored at 2 °C after exposure to 20 °C (control), 40 °C (HAT₄₀), 45 °C (HAT₄₅) or 50 °C (HAT₅₀) hot air for 3 h. The effects of HAT on firmness, disease incidence, soluble solid content (SSC), titratable acidity (TA), respiration, ethylene production, pectic substances and activities of pectinmethylesterase (PME) and polygalacturonase (PG) were examined. RESULTS: HAT inhibited decrease in firmness, SSC and TA and retarded increase in disease incidence, while it promoted decrease in respiration rate and ethylene production. HAT suppressed the activities of PME and PG, which resulted in delaying the depolymerisation of chelator‐soluble and alkali‐soluble pectic substances and reducing the increase in water‐soluble pectic substances compared with control fruits. CONCLUSION: The present findings show that HAT can maintain postharvest quality and delay ripening of Chinese bayberry fruit and suggest that HAT could be considered for commercial use to extend the postharvest life of Chinese bayberry fruit. Copyright © 2008 Society of Chemical Industry     

Effects of short‐term N2 treatment on quality and antioxidant ability of loquat fruit during cold storage

BACKGROUND: Loquat fruit is susceptible to microbial decay and quality deterioration during postharvest distribution. In this study, in a search for effective alternatives to methods currently used for quality maintenance, the effects of short‐term pre‐storage N₂ treatment on the quality and antioxidant ability of loquat fruit were investigated. RESULTS: Loquat fruits were exposed to 100% N₂ for 6 h at 20 °C and then stored at 5 °C for 35 days. Short‐term N₂ treatment significantly delayed the increase in fruit decay rate and decreases in total soluble solid and titratable acidity contents, thereby maintaining better eating quality and extending the storage life of fruits. It also markedly delayed increases in membrane permeability, malondialdehyde content and superoxide anion production rate. In addition, N₂‐treated fruits exhibited significantly higher superoxide dismutase and catalase activities and lower lipoxygenase activity than control fruits. CONCLUSION: Short‐term pre‐storage N₂ treatment effectively reduced fruit decay and maintained quality in loquat fruits during cold storage. The reduction in fruit decay and quality deterioration by the treatment was correlated with enhanced antioxidant ability and reduced lipid peroxidation. Copyright © 2009 Society of Chemical Industry     

Movement of Cryptolestes ferrugineus out of wheat kernels and their mortalities under elevated temperatures

The rusty grain beetle, Cryptolestes ferrugineus (Coleoptera: Laemophloeidae), is a common pest in stored grain. The present study examined the time taken for larvae of C. ferrugineus to exit wheat kernels at 40–55 °C, stage-specific mortality at 40–60 °C, and whether a combination of heat and shaking could be used to control this pest. The average time larvae took to leave wheat kernels ranged from 130 min at 40 °C to 6 min at 55 °C. At 55 °C and 6 min exposure, 81 ± 2% of larvae exited the kernels, 10 ± 1% of larvae were alive inside kernels and 9 ± 2% were dead inside kernels. Under constant temperature of 55 °C, less than 5% of the larvae escaped in 2 min, but under the rising temperature of 40, 45, 50 and then to 55 °C, over 50% of the larvae escaped in 2 min at 55 °C. The time taken to reach 50 and 95% mortality was similar for all stages. Higher temperatures resulted in higher mortality in a shorter exposure time. The longest LT₉₅ for pupae was 6480, 293, 50 and 3.7 min at 45, 50, 55 and 60 °C, respectively. At 55 °C for 88 min, combined with shaking gave 88% control in a 100-g wheat bulk. Time needed to kill eggs and pupae were much longer than the time needed to force larvae out of kernels. Therefore, the combination of heat and shaking would only provide partial disinfestation of C. ferrugineus.     

Photochemical Treatment to Improve Storability of Fresh Strawberries

We investigated the effect of pre-storage exposure to shortwave ultra-violet (UV-C) light on the decay and quality of fresh strawberries. Fresh strawberries (cv. Kent, 25% to 50% red) were exposed to UV-C at doses of 0.25 and 1.0 kJ/m² and stored at 4 °C or 13 °C. UV treatment controlled the decay caused by Botrytis cinerea at both temperatures and extended the shelf-life of the fruits by 4 to 5 d. UV-treated fruits had a lower respiration rate, higher titratable acidity and anthocyanin content, and were firmer than the untreated fruits. The percentage of free sugars increased faster in UV-treated fruits at the beginning of the storage period. A lower electrical conductivity in fruits treated with 0.25 kJ/m² suggests a slower rate of senescence compared to the control. A higher electrical conductivity observed with 1.0 kJ/m²suggests damage to the fruits. Overall, UV treatment at 0.25 kJ/m² appears to slow down the ripening and senescence of strawberry fruits stored at 4 °C.     

Influence of moderate heat pre-treatments on physical and chemical characteristics of kiwifruit slices

The effect of mild heat treatments, applied to whole kiwifruit, on physical characteristics and chemical composition of minimally processed fruit was studied. Fruits were subjected to heat treatments at 45 °C for 25 and 75 min, cooled for 24 h, minimally processed and stored at 4 °C for 12 days. Heat-treated fruits showed increased respiration rates in the first 2 days of storage. Samples colour was marginally affected either by heat treatments or by minimal processing. The application of heat treatments leads to an increment of slices firmness due to cross linking between demethylated galacturonic acid chains and endogenous calcium. An increment in sucrose, L-malic, citric, quinic and ascorbic acids was observed as a consequence of applied heat treatments. Heat treatment at 45 °C for 25 min applied to whole fruits allows quality retention of minimally processed fruit during 9 days at 4 °C.     

Hot water bath treatments assisted by microwave energy to delay postharvest ripening and decay in strawberries (Fragaria × ananassa)

BACKGROUND: A lab‐scale approach using microwave (MW)‐assisted hot water treatments was developed and tested to assess the potential of this heating method to delay postharvest ripening and decay in strawberries. Strawberries (Fragaria × ananassa) immersed in water were exposed to microwaves at a frequency of 2450 MHz for 3 min at 514 W or 1 min 50 s at 763 W to reach an average temperature of 43.8 ± 0.6 °C at the fruit centre. Another batch was treated in hot water at 45 °C for 15 min, and a final batch was not treated (control). RESULTS: After 9 days of refrigerated storage (3 °C and 90% relative humidity), all heat‐treated strawberries showed significant retention of quality parameters such as colour and firmness and significantly lower yeast and mould populations (P < 0.05). CONCLUSION: Strawberries subjected to MW‐assisted hot water treatments showed significantly better retention of lightness compared with conventionally treated berries. A short (1 min 50 s) treatment at 763 W was the best choice to prevent strawberry decay. Copyright © 2011 Society of Chemical Industry     

Inactivation of Escherichia coli with Power Ultrasound in Apple Cider

The use of acoustic energy to secure apple cider safety was explored. Inactivation tests were performed with Escherichia coli K12 at 40 °C, 45 °C, 50 °C, 55 °C, and 60 °C with and without ultrasound, followed by a validation test with E. coli O157:H7 at 60 °C. The cell morphology was observed with environmental scanning electron microscopy for samples treated at 40 °C and 60 °C. Physical quality attributes of the apple cider (pH, titratable acidity, °Brix, turbidity, and color) were compared for treated samples. The inactivation tests showed that sonication increased E. coli K12 cell destruction by 5.3‐log, 5.0‐log, and 0.1‐log cycles at 40 °C, 50 °C, and 60 °C, respectively. The additional destruction due to sonication was more pronounced at sublethal temperatures. At the lethal temperature of 60 °C, the rate of death by ultrasound was not significantly different compared with the thermal‐alone treatment. The inactivation of E. coli K12 with heat was described by 1st‐order kinetics, especially at 50 °C and 60 °C. For ultrasound treatments, concave upward survival curves were observed, which had a shape factor in the range of 0.547 to 0.720 for a Weibull distribution model. Extensive damage for ultrasound treated E. coli K12 cells, including cell perforation, was observed. Perforation is a unique phenomenon found on ultrasound‐treated cells that could be caused by liquid jets generated by cavitation. Titratable acidity, pH, and °Brix of the cider were not affected by ultrasound treatment. Minor changes in color and turbidity for ultrasound treated samples, especially for sonication at 40 °C for 17.7 min, were observed.     

Postharvest Changes in Antioxidant Capacity,Enzymatic Activity,and Microbial Profile of Strawberry Fruits Treated with Enzymatic and Divalent Ions

Strawberry fruits are highly perishable and cannot be stored for more than 1–2 days at ambient condition. Therefore, it was thought desirable to enhance the shelf-life of harvested strawberry using pectin methyl esterase and divalent ions. The freshly harvested fruits of strawberry were treated with different concentrations of pectin methyl esterase (50–300 units) and divalent calcium ions (calcium chloride) for 5–30 min using response surface methodology—Box–Behnken design. The treated and untreated (control) fruits were packed in plastic punnets and stored at two different temperature conditions, viz., 7 °C with 80 % relative humidity (RH) and 25 °C with 60 % RH, for evaluating the shelf-life. Appropriate physico-chemical and microbiological parameters were determined at alternate days during their storage. Overall firmness and color values (L, a, b) decreased, while physiological weight loss (percent) increased during their storage. No food-borne pathogens, viz., Salmonella, Staphylococcus, and coliforms, were observed during storage. Total antioxidant capacity and ferric reducing antioxidant power decreased, while lipoxygenase and polyphenol oxidase activities increased during storage at 7 and 25 °C. The shelf-life of treated strawberry was found to be 10 days at 7 °C as against 6 days for control fruits under similar conditions. The treated strawberry had shelf-life of 2 days compared to 1 day for control maintained at 25 °C.     

Changes in cuticle compositions and crystal structure of ‘Bingtang’ sweet orange fruits (Citrus sinensis) during storage

Changes in composition and structure of orange cuticle during storage at 4°C or 25°C for 40 days were investigated. The total epicuticular wax content of fruits increased during storage at 4°C for 30 days and then decreased as storage time prolonged to 40 days, while it increased continuously at 25°C for 40 days. The total intracuticular wax content of fruits increased to 9.70 μg cm^?2 stored at 4°C for 10 days and then decreased to 6.74 μg cm^?2 for 40 days. The total intracuticular wax content of fruits was decreased to 5.17 μg cm^?2 stored at 25°C for 10 days and then increased to 10.06 μg cm^?2 for 30 days. Fatty acids were the most abundant component of the epicuticular wax and terpenoids were restricted to the intracuticular wax. Terpenoids were restricted to the intracuticular wax, and their amounts in the fruit stored at 4°C increased continuously during the first 20 days of storage at 4°C and then decreased as storage time increased up to 40 days. Although significant changes were found in the contents of the cutin monomer, their proportions did not change significantly during storage at 4°C or 25°C. Size of wax platelets crystals wax increased during storage of fruits at 4°C up to 30 days; however, the crystals were degraded and decreased as storage prolonged to 40 days. Furthermore, the shape of the fruit cuticle surface wax crystals changed from flattened platelets to small granulelike after storage at 25°C for 20–40 days. The obtained results provide detailed information about the changes in orange cuticle occur during storage at varying temperatures, which may help in preserving the quality of citrus fruits during storage.     

Preparation and Properties of Physically Modified Banana Starch Prepared by Alcoholic‐Alkaline Treatment

Granular cold‐water‐soluble (GCWS) starches were prepared from banana starch treating it with 40 and 60% aqueous ethanol at two controlled temperatures (25 and 35 °C). GCWS starches prepared at 25 °C and with 40 and 60% aqueous ethanol had the lowest cold‐water solubility, that prepared with 40% aqueous ethanol at 35 °C and stored at room temperature showed low tendency to retrogradation, as assessed by transmittance. Solubility and swelling profiles were similar for GCWS starches and the freeze‐thaw stability of GCWS starches was increased as compared with native starch. The apparent viscosity of GCWS banana starches was higher than that of its native starch counterpart.     

HEAT SHOCK REDUCES BOTH CHILLING INJURY AND THE OVERPRODUCTION OF REACTIVE OXYGEN SPECIES IN YELLOW PITAYA (HYLOCEREUS MEGALANTHUS) FRUITS

ABSTRACT

Yellow pitaya fruits were heat‐shocked (25C/24 h), stored at 2C for 14 days and then held for 13 days at 18C. Chilling injury (CI), respiration rate, hydrogen peroxide (H₂O₂), superoxide anion radical (O₂•^‐) and lipid peroxidation product (LPP) levels were measured. Pitting and browning were detected in the control fruit when moving from 2 to 18C. Minor CI symptoms were found in the treated fruit. There was a rapid increase in the levels of H₂O₂, O₂•^‐ and LPP in the control fruits when moved to 18C. At the end of the storage, the levels of these compounds were 4.5‐, 4.9‐ and 6.2‐fold higher, respectively, in the control than in the treated fruit. The results suggest that the reactive oxygen species levels in the heat‐shocked fruit were adequate for normal ripening, while the levels in the control group were excessively high, inducing CI symptoms.

PRACTICAL APPLICATIONS

Refrigeration is a technique that can be used to extend shelf life of yellow pitaya fruit. Nevertheless, these fruits are susceptible to chilling injury. This research was developed to study the effect of heat shock treatment on the preservation of whole yellow pitaya fruit during refrigeration. Preheating fruit at 25C for 24 h before storage at 2C for 14 days prevented chilling injury during shelf life at 18C. Thus, the combination of heat shock and refrigeration delays the senescence of yellow pitaya fruit and therefore improves its shelf life. Furthermore, this treatment could be useful during quarantine treatments of fruit fly‐infested fruit.

     

Effects of Soaking with Natural Additives in Combinations with Vacuum or Modified Atmosphere Packaging on Microbial Populations and Shelf Life of Fresh Truffles (Chinese Tuber Indicum)

The objective of this study was to evaluate the relative effects and interactions of combined soaking treatment using citric acid (CTA) and apple polyphenol (APP) at mild heating temperatures for the inactivation of the external and internal microflora (mesophilic aerobic bacteria, mesophilic anaerobic bacteria, and fungi) in Chinese Tuber indicum, as well as to analyze the microbiological and sensory changes under modified atmosphere packaging (MAP)‐ and vacuum atmosphere packaging (VAC)‐packed Chinese T. indicum stored at 4 °C for up to 55 d. Chinese T. indicum was soaked with CTA and APP alone or in combination for 10, 20, and 30 min at 35, 45, and 55 °C. A disinfection method using CTA and APP (3% CTA + 3% APP for 20 min at 45 °C) was obtained. Under this set of combination, the experimental values of microbial counts of mesophilic aerobic bacteria, mesophilic anaerobic bacteria, and fungi were 2.31 ± 0.4 log CFU/g, <1.0 log CFU/g, and <1.0 log CFU/g, respectively. Through the analysis of sensory qualities and microbial populations for MAP‐ or VAC‐packed Chinese T. indicum, the shelf life of soaked truffles was prolonged to 45 or 40 d, respectively. The synergistic effect of CTA and APP may provide valuable insight into the reduction of microorganisms on fresh truffles.     

Effects of Ultraviolet Light (UV‐C) and Heat Treatment on the Quality of Fresh‐Cut Chokanan Mango and Josephine Pineapple

The effects of ultraviolet (UV‐C) and medium heat (70 °C) treatments on the quality of fresh‐cut Chokanan mango and Josephine pineapple were investigated. Quality attributes included physicochemical properties (pH, titratable acidity, and total soluble solids), ascorbic acid content (vitamin C), antioxidant activity, as well as microbial inactivation. Consumers’ acceptance was also investigated through sensory evaluation of the attributes (appearance, texture, aroma and taste). Furthermore, shelf‐life study of samples stored at 4 ± 1 °C was conducted for 15 d. The fresh‐cut fruits were exposed to UV‐C for 0, 15, 30, and 60 min while heat treatments were carried out at 70 °C for 0, 5, 10 and 20 min. Both UV‐C and medium heat treatments resulted in no significant changes to the physicochemical attributes of both fruits. The ascorbic acid content of UV‐C treated fruits was unaffected; however, medium heat treatment resulted in deterioration of ascorbic acids in both fruits. The antioxidants were enhanced with UV‐C treatment which could prove invaluable to consumers. Heat treatments on the other hand resulted in decreased antioxidant activities. Microbial count in both fruits was significantly reduced by both treatments. The shelf life of the fresh‐cut fruits were also successfully extended to a maximum of 15 d following treatments. As for consumers’ acceptance, UV‐C treated fruits were the most accepted as compared to their heat‐treated counterparts. The results obtained through this study support the use of UV‐C treatment for better retention of quality, effective microbial inactivation and enhancement of health promoting compounds for the benefit of consumers.     

Effect of preharvest spray of plant growth regulators on size, composition and storage behaviour of Coorg mandarins [Citrus reticulata (blanco]

Mandarin orange trees bearing the monsoon crop* were sprayed with CLPA and 2,4,5-T and NOA 8 weeks before harvest. Fruits treated with 2,4,5-T (25 and 50 ppm) and CLPA (25 ppm) had 34, 35 and 28% more weight, respectively, than the control and also had higher percentage of juice and reduced pomace content with a corresponding increase in peel moisture. When stored for 35 days at 42-45°F and 85-90% R.H., the treated fruits suffered less physiological loss in weight, a greater percentage of them being in marketable condition as compared with the untreated ones. The vitamin C loss also was much less during storage. The treatments had little effect on respiratory activity, although there was a peak on the fourteenth day of storage, possibly a respiratory climacteric.     

Combined effects of postharvest heat treatment and chitosan coating on quality of fresh‐cut mangoes (Mangifera indica L.)

The influence of heat treatment combined with coating on sensory quality, physico‐chemicals characteristics (firmness, colour, pH, titratable acidity, total soluble solids and total carotenoids content) and microbiological quality of fresh‐cut mangoes were studied. Whole mangoes (Mangifera indica cv ‘Tommy Atkins’) were subjected to hot water dipping (HWD) at 50 °C for 30 min and cooled for 15 min. Heated and unheated fruits were minimally processed and mango cubes were coated with chitosan solution (0.25% w/v) dissolved in 0.5% (w/v) citric acid, and stored for 9 days at 6 °C under ambient atmosphere. This study showed that both HWD 50 °C for 30 min and chitosan coating, either alone or combined, did not affect the taste and the flavour of mangoes slices. The chitosan coating combined with HWD or not inhibited the microbial growth for 9 days at 6 °C. Indeed chitosan coating was used for his antimicrobial proprieties. HWD 50 °C for 30 min was the beneficial treatment to maintain firmness and colour during 9 days at 6 °C.     

Effect of 1-methylcyclopropene on tomato flavour components, shelf life and decay as influenced by harvest maturity and storage temperature

BACKGROUND: In Florida, tomatoes are harvested green (GR), which includes mature green (MG) and immature green (IG) fruits, and stored at low temperature (13 °C), resulting in poor flavour. Flavour improvement might be achieved if fruits were harvested with some colour (to eliminate IG fruits) and/or stored at higher temperature with the ripening inhibitor 1‐methylcyclopropene (1‐MCP). RESULTS: ‘Florida 47’ tomatoes were harvested at GR (MG + IG), breaker (BR), turning (TR) and pink (PK) stages, treated (+) or not (?) with 1‐MCP and stored at 13 and/or 18 °C. 1‐MCP treatment resulted in a gain of up to 6 days of shelf life depending on harvest maturity and storage temperature. Storage at 18 °C rather than 13 °C resulted in an increase in internal red colour, soluble solids (SS)/titratable acidity (TA) ratio and levels of many volatiles studied. The shelf life of BR (+) MCP fruits stored at 13 °C was similar to that of MG fruits. IG fruits exhibited lowest levels of SS, TA and ten volatiles compared with BR fruits. CONCLUSION: Harvesting tomatoes with colour (BR), thereby eliminating IG fruits, and treating with 1‐MCP resulted in better quality with adequate shelf life. Published 2011 by John Wiley & Sons, Ltd.     

Influence of Immature Fruits on Strawberry Jam Quality and Storage Stability

Jam was prepared from two strawberry cultivars, ‘Cardinal’ and A-5344, which were suited to once-over machine harvest. For jam manufacture, puree from ripe (R) and immature (IM) fruits were combined in the following blends: 25% R + 75% IM, 50% R + 50% IM, 75% R + 25% IM, and 100% R. Initially and after storage at 2°C for 6 and 12 months, jam color and flavor of all mixtures were acceptable. Jam made from 25 and 50% ripe fruit of A-5344 had unacceptable color after 6 and 12 months, respectively, at 25°C. All jam stored at 35°C was unacceptable after 6 months. Color acceptability was more dependent on total anthocyanin content than the percentage of ripe puree used in jam manufacture.     

Influence of Sulfur Dioxide Generators on Red Raspberry Quality During Postharvest Storage

Hand harvested ‘Meeker’ red raspberries were held at 4°, 14°, and 25°C for up to 12 days using two types of sulfur dioxide generating pads. Visual mold ratings were lower for fruits held with either SO₂ generator, but Howard Mold Count did not differ between the generators and the control fruits. Fruits stored with the generators were brighter and redder in color than controls when stored at 4°C. Irregular bleaching of anthocyanins from drupelets occurred when fruits were stored at 14°C or higher. Use of SO₂ generators is not recommended for fresh market fruit or nonheat treated processing fruit due to the bleaching and due to SO₂ residues in the fruit.