表面等离子体共振传感检测方法研究

为了找到表面等离子体共振(SPR)检测物体的最佳方法,基于SPR,通过Matlab进行了一系列仿真。对检测物质的可行性进行了讨论,得出SPR能在一定范围内检测物质。对影响SPR现象的因素进行了仿真,得出达到最佳共振效果的参数值。分别对角度调制和波长调制方法进行仿真比较,分析了二者检测不同折射率样品的可行性和特点,选择出了较佳的检测方法。结果得出:达到最佳共振效果的参数值是金膜厚度为45 nm,入射光波长为632.8 nm,棱镜折射率根据实验条件和样品综合选定。角度和波长调制均能较好地检测不同折射率的样品,角度调制检测分辨率低于波长调制。     

表面等离子体共振生物传感器的研究现状

表面等离子体共振生物传感器是分子生物学与光学、电化学、微电子学等相结合的产物,是分子生物学信息分析检测最重要的技术之一。本文简要介绍了表面等离子体共振生物传感器的结构原理及研究方向。     

表面等离子体共振传感器的仿真

为了得到基于相位检测、角度检测和波长检测的表面等离子体共振(SPR)生物传感器的高检测精度,利用Matlab建立了Kretschmann模型SPR传感器的数值仿真软件.系统地进行了棱镜折射率、测试介质折射率、金膜的厚度等因素对3种不同检测方式的SPR曲线的影响.理论分析结果表明:角度调制时与介质折射率增加时,SPR角也相应增加,相位检测对传感层的折射率具有选择性.     

光纤表面等离子体共振传感器研究进展

以光纤传输技术的表面等离子体共振技术有机结合的光纤表面等离子体共振传感器，是实现微量生物和化学活性物质定量测定的重要技术之一。介绍了表面等离子体共振的基本原理，表面等离子体共振光纤传感器的制作及应用的研究进展。     

表面等离子共振Ag-SnO_2薄膜光化学气体传感器

提出一种新型的Ag-SnO2复合膜H2S光化学表面等离子共振(SPR)气体传感器结构。结构中使用一只金红石材料作成的棱镜作为耦合棱镜,在棱镜的底边采用射频溅射技术制作Ag-SnO2复合膜(CuO为掺杂质),Ag膜和SnO2膜的厚度分别为50 nm和100 nm,在复合膜上设置待测气体的样品池。在白光入射激励下,复合膜的分界面发生SPR现象。实验结果表明:SPR的波长与H2S气体的体积分数基本呈线性关系。     

表面等离子体共振成像技术的进展与应用

简单介绍了表面等离子体共振成像(SPRI)的原理之后,比较详细地介绍近年来其应用情况及相关技术的发展.     

光纤表面等离子体波传感器测量液体折射率的研究

针对表面等离子体波共振现象产生的原理和激发条件进行了阐述.通过自行设计的一套光纤表面离子体波传感器测试系统,测定了从0%～70%的不同浓度甘油水溶液折射率与共振波长的关系,实验结果具有较好的重复性并和理论分析相吻合.由此得到一种基于光纤SPR传感技术的液体折射率测量方法.     

胶体表面等离子体共振放大效应用于抗体的检测

在透明介质的表面,利用胶体金单层和蛋白质修饰胶体金的表面等离子体共振放大效应,发展了一种检测生物大分子的新方法,并利用该方法检测了不同浓度的羊抗人IgG.实验结果表明,该方法不受探针和目标分子体积的影响,不需要昂贵的实验仪器,操作简单,通用性好,并有望制成点阵芯片,实现高通量检测.     

基于表面等离子体共振的双通道光子晶体光纤温度传感器

提出了一种高灵敏度双待测物质通道的宽温度范围区间的基于表面等离子体共振的光子晶体光纤(SPR-PCF)型传感器.利用SPR技术,通过纤芯损耗谱测量共振波长的变化达到对不同温度测量的目的,并且结构简单,易于测量操作,其工作的波长范围可以调节.实验结果表明:其温度测量区间为-80 ～80℃,可以得到最大光谱灵敏度3 nm/...     

基于表面等离子体共振传感器的尿微量白蛋白检测

检测尿微量白蛋白(MAU)可对轻微肾脏损害进行早期诊断,也可对高血压患者可能发生的心脑血管事件进行预测。实验将抗体通过巯基自组装固定于Au膜表面,研制了一种快速检测MAU的表面等离子体共振(SPR)生物传感器。结果表明:直接检测法可以检测到0.3μg/L的MAU,而纳米Au放大法检测限可以低至0.03μg/L。     

表面等离子共振传感器的研究进展

表面等离子体是在金属和电介质交界面上所形成的电荷层,在电磁波的激励下表面等离子体发生共振现象,影响电磁波的传播。根据这一原理制作的表面等离子共振传感器可对化学和生物等量进行探测。介绍了表面等离子共振传感器的工作原理和最新研究进展。由于具有体积小、准确度高、抗电磁干扰能力强和可用于遥测等优点,SPR传感器将有广泛的应用前景。     

基于SPR的光纤传感器的研究与实验

基于表面等离子体共振（SPR）的光纤传感器不仅具有SPR高灵敏性的特点,还发挥了光纤本身的诸多优点,具有广泛地研究和应用价值。在分析了光纤SPR原理的基础上,设计并实现了光纤SPR传感器,给出了系统结构模型。通过对不同质量分数的甘油溶液进行测试,获得了SPR光谱共振波长等随待测环境介质折射率变化的有效信息,证实了使用这种传感器进行环境介质检测的可行性。     

Sensitivity of surface plasmon resonance sensors based on metallic columnar thin films in the spectral and angular interrogations

Surface Plasmon Resonance (SPR) from metallic Columnar Thin Films (CTFs) of porosity as high as 0.5 was experimentally and theoretically investigated. The CTF layers were prepared by the Glancing Angle Deposition (GLAD) method. The SPR features were investigated in both the angular and the spectral modes. In the angular interrogation, increasing the porosity causes broadening to the dip width, shift to larger resonance angles, and increase of the sensitivity to analyte refractive index (RI) changes by about threefold compared with closed metal films. In the spectral interrogation, on the other hand, the resonance wavelengths are red-shifted for porous films; hence their spectral sensitivities are higher than those of closed films under the same experimental conditions. Nevertheless, the sensitivity behavior versus the resonance wavelength is similar to that of SPR sensors based on dense film layers. The shapes of the nanostructures constituting the CTF are described as ellipsoidal inclusions in which the effective permittivity dyadic of the composite material is calculated using the Bruggeman formalism with exact depolarization dyadics. The correlation between the sensitivity enhancement and the electromagnetic field intensity at the interface between the metallic film and the analyte was examined. Electromagnetic fields analyses were performed using the general 4 × 4 propagation matrices of general homogenous biaxial layers.     

Manufacture of robust surface plasmon resonance fiber optic based dip-probes

Surface plasmon resonance (SPR) spectroscopy has been conducted on both prism and fiber optic (FO) based sensors for several years. This technique measures the refractive index (RI) of a solution or layer adsorbed to a thin (50 nm) Au layer on the sensor substrate. To date a succinct set of protocols have not been published regarding the optimization of fiber-based SPR dip-probe sensors. Such sensors would allow application of SPR to a wider variety of applications. This paper focuses on consideration of the choice of fiber, isolation of the mirror from the sensing area, and orientation of the probes in the metal layer sputter deposition chamber in the manufacture of SPR dip-probes for reproducibility and robustness. Optimization of the process yields sensors with a batch to batch reproducibility as low as 0.5 nm in the location of the SPR spectral minima. Further study of RI measurements by the same probe over 2 months show these SPR dip-probes have a long shelf-life. A selection of probes was exposed to various solutions to monitor their drift. The data shows the probes’ response indicated a lowering of the RI measured over a period of 3 or 7 days depending on the probe type. Evidence of surface porosity and damage upon exposure to hydrothermal water seems to indicate these sensors are prone to chemical attack. Further research is needed to characterize this attack and allow creation of more robust sensors.     

Surface functionalization for self-referencing surface plasmon resonance (SPR) biosensors by multi-step self-assembly

Recently, a novel SPR sensor with on-chip referencing has been realized. In this sensor, one-half of the gold sensing surface is covered with a high refractive index overlayer of tantalum pentoxide (Ta₂O₅). When polychromatic beam illuminates the sensing surface, surface plasmon resonance in the areas with and without the overlayer occur at different wavelengths. Therefore, the reflected light exhibits two dips associated with SPRs in those two areas. When functionalized properly, one of the areas can be used as a specific sensing channel for detection of specific bio-interactions and the other can act as a reference channel for compensation for background refractive index fluctuations. In this paper we present a new functionalization approach for these mixed architecture chips. The gold side of the chip is functionalized with a mixed self-assembled monolayer of polyethylene oxide (PEO) and biotin terminated (BAT) thiols whereas the Ta₂O₅ side is coated with PEO terminated silanes. The PEO terminated thiols and silanes serve as a protein resistant background, while the biotin terminated thiols are used to bind streptavidin, which in turn immobilizes biotinylated antibodies. Hence, the gold side of the chip is used for the binding and detection of target analytes and the Ta₂O₅ side functions as a reference channel that monitors bulk refractive index changes and temperature drift. We have studied human chorionic gonadotropin (hCG) as a model system, currently detecting down to 5 ng/ml. In addition, we demonstrate the power of the on-chip reference channel for compensating for refractive index changes and eliminating false alarms.     

光纤表面等离体激元共振传感器中光传播特性的理论分析

光纤表面等离体激元共振传感器是一种易于小型化的SPR传感器,常用于开发便携式传感检测设备,也可用于远距离实时在线检测.在本研究中,采用特制光纤作为导光介质来制作一种新的光纤SPR传感器.由于光纤中光传播相当复杂.仅凭过往经验来设计传感器将不能保证其检测精度.因此,针对本研究中光纤的特点,建立了光纤中光传播模型,并结合菲涅耳公式推导出该类光纤SPR传感器中光总反射系数与光波长的关系,最终绘制出理论SPR曲线并计算出共振波长.研究结果为该类光纤SPR传感器的设计及光谱分析提供了重要理论依据.