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1.
饮料中的蛋白-多酚混浊   总被引:3,自引:0,他引:3  
了解混浊活性(HA)蛋白和多酚的性质与相互作用,有助于生产出更好的饮料产品,使用更好的分析方法及稳定手段。  相似文献   

2.
引起啤酒、白酒和澄清果汁出现混浊或沉淀的原因很多,最常见的是由蛋白质———多酚的相互作用引起的,但这些饮料一般能稳定存在,并能延缓蛋白质———多酚混浊物形成开始的时间。10多年前已有关于促使蛋白质或多酚成为混浊激活物(HA)方面的报道(Asanoetal,1982;Moll,1987),但近来的研究发现,饮料中两者的比例对混浊的形成数量起着重要的作用(Siebertetal,1996c),这发现使我们能更好地理解一些检测HA种类的分析方法及其稳定的机制。在鉴别HA物质的特性方面,我们已经做子大量的工作(Moll,1987)。啤酒中的…  相似文献   

3.
1前言啤酒的成分复杂,稳定性不强,易产生混浊沉淀。最常见的啤酒非生物混浊是蛋白质混浊。形成蛋白质混浊的原因在于啤酒中有两种主要成分,即蛋白质和多酚物质。在啤酒中,某些多酚物质("混浊活性多酚")可以与蛋白质("混浊活性蛋白")结合形成复杂的复合物,且二者存在一定  相似文献   

4.
饮料中蛋白质——多酚的作用机制   总被引:3,自引:0,他引:3  
蛋白质-多酚的相互作用是引起饮料混浊的重要原因之一。蛋白质具有混浊形成活性主要取决于它的脯氨酸含量,多酚形成混浊的能力与其分子结构有关,蛋白质与多酚的比例强烈影响到饮料中混浊的形成量。文中叙述了饮料中蛋白质-多酚的作用机制和最新进展。  相似文献   

5.
啤酒多酚-蛋白质沉淀及低温酒精冷混浊预测啤酒保质期   总被引:1,自引:1,他引:0  
王娟  郝俊光  顾国贤 《酿酒》2003,30(4):51-53
啤酒的混浊问题比较复杂,但通常是由多酚—蛋白的复合物引起的非生物混浊;低温酒精冷混浊试验可以快速地预测啤酒保质期,该法简捷,测定结果预见性强,填补国内无快速预测啤酒保质期的技术空白。  相似文献   

6.
吴丹  陈健初 《食品科学》2004,25(1):145-146
多酚蛋白复合引起的混浊现象是啤酒,果汁需要着重解决的问题之一。本文探讨了标准曲线法测定啤酒,果汁中混浊活性蛋白的可行性。结果表明,PVP的浓度在0.000~0.100g/L范围时,反应产物的浑浊度与PVP浓度成良好的线性关系。  相似文献   

7.
綦菁华  蔡同一  于同泉 《食品科学》2007,28(11):106-109
采用18000r/min冷冻(-4℃)离心20min的方法处理经二次混浊处理了3个批次的果汁,测定上清液的相应指标,与未处理的果汁做空白对比,由相应的成分的变化,确定混浊物的成分。结果表明:和二次混浊有关的多酚主要是原花青定的聚合体;表儿茶素、儿茶素小分子的酚类不直接参与二次混浊,却是形成二次混浊的前体物质;和二次混浊有关的蛋白质疏水性氨基酸含量高,其中脯氨酸含量大。  相似文献   

8.
采用疏水作用色谱PhenylSepharoseCL-4B和固相萃取C18柱分离纯化了浓缩苹果汁和鲜榨苹果汁中与混浊有关的活性蛋白质。经过SDS-PAGE,结果表明浓缩苹果汁和鲜榨苹果汁中的活性蛋白质为同一类蛋白,均为疏水性蛋白质,其分子量为36kDa。  相似文献   

9.
《食品工业科技》2006,(01):95-96
采用疏水作用色谱PhenylSepharoseCL-4B和固相萃取C18柱分离纯化了浓缩苹果汁和鲜榨苹果汁中与混浊有关的活性蛋白质。经过SDS-PAGE,结果表明浓缩苹果汁和鲜榨苹果汁中的活性蛋白质为同一类蛋白,均为疏水性蛋白质,其分子量为36kDa。   相似文献   

10.
植物多酚作为一种天然抗菌剂,因具有良好的抗氧化和抗菌活性,在抗菌膜材料领域倍受关注。针对植物多酚-蛋白复合抗菌膜的研究现状,该文综述了蛋白抗菌膜的制备原理、性能和应用,重点阐述植物多酚对蛋白膜抗菌性、抗氧化性、机械性能、阻隔性能、微观结构等理化性质的影响,并对植物多酚-蛋白抗菌膜的发展前景进行了展望,以期为深入挖掘植物多酚-蛋白抗菌膜应用范围提供参考。  相似文献   

11.
    
Resveratrol has been identified as a wine component related to moderate wine consumption and a reduction in serum cholesterol levels. Processes such as wine fining that result in loss of resveratrol during winemaking are therefore of interest, and led to these present studies. A number of agents were compared and were found to lower resveratrol levels in all wines to some extent. Results from two studies (1996 and 1997) are reported. The standard addition method was used in combination with High Pressure Liquid Chromatography to calculate resveratrol levels. In Study 1 (1996), recommended maximum levels of all fining agents (Level 3), bentonite, egg white, gelatin + kieselsol and polyvinylpolypyrrolidone (PVPP), lowered resveratrol levels significantly compared to controls (Level 0). Nevertheless, addition of fining agents at Level 0 resulted in resveratrol levels that were significantly higher than those at Level 3, but resveratrol levels in wine from Level 0 were not significantly different from Level 1. In Study 2 (1997), carbon + egg white, and gelatin + kieselsol fining was studied, and their effects differed according to grape variety. Least removal of resveratrol by carbon fining occurred in wine from Cabernet Sauvignon (Vitis vinifera) whereas most removal occurred in wines from Cynthiana (Vitis aestivalis) and Noble (Vitis rotundifolia). Resveratrol levels of control wine were significantly higher than resveratrol levels of wine treated with recommended maximum addition of fining agent in all varieties. Taken overall, any addition of any fining agent lowered resveratrol levels in all wines to some extent, but complex interactions between fining agent and wine variety resulted in different regression trends. While recognising the constraints set by our particular data that could reflect unique circumstances, we are nevertheless able to infer from these trends that low levels of fining agents can be used without statistically significant loss of resveratrol.  相似文献   

12.
复合保健茶饮料的工艺研究   总被引:1,自引:0,他引:1  
以茶叶及几种药食两用植物为原料研制了一种复合保健茶饮料,实验采用脱酚工艺及添加抗坏血酸,解决了饮料混浊沉淀及褐变问题。  相似文献   

13.
    
In this paper we investigate the interaction of phenolics extracted from olive oil with different food proteins (sodium caseinate, bovine serum albumin, β‐lactoglobulin and gelatin). Binding parameters are estimated using different experimental techniques: gel filtration, HPLC, isothermal titration calorimetry and NMR diffusion measurements. For comparison, the binding properties of gallic acid and tannic acid are also studied. The affinity of olive oil phenolics for the different food proteins is found to be relatively weak (compared with tannic acid). Binding constants are measured for the different phenolics in the extract: tyrosol and hydroxytyrosol do not (or very weakly) bind to the proteins, whereas other phenolics in the extract had binding constants of the order 102–104 M ?1. The binding parameters determined have been discussed in relation to the possible effect of proteins on sensory properties (bitterness) of food emulsions containing olive oil. Copyright © 2004 Society of Chemical Industry  相似文献   

14.
综述了饮料用乳化香精的定义、特点、浊度和稳定性的原理和影响因素、主要原料、乳化工艺、分析方法及其进展。  相似文献   

15.
对樱桃中的多酚、花色苷和花青素等抗氧化活性因子以及樱桃果醋果酒的制作工艺进行综述,并对樱桃的发展前景进行了预测。  相似文献   

16.
孙蕾  沈群 《食品工业科技》2012,33(13):220-223,227
本文主要对小米饮料的淀粉液化、糖化的最佳反应条件和稳定剂对小米饮料稳定性的影响进行了探讨,结果表明小米浸提液的液化最佳条件为:α-淀粉酶的加酶量6U/g淀粉,作用时间90min,作用温度70℃,pH6.5;糖化的最佳条件为:β-淀粉酶的加酶量100U/g淀粉,作用时间60min,作用温度60℃,pH5.5;稳定剂的最佳配比为:0.5%的阿拉伯胶、0.07%的瓜儿豆胶、0.07%的黄原胶、2.1%的蔗糖。  相似文献   

17.
    
Background and Aims: Bentonite fining to remove protein is the most widely used treatment to prevent protein haze in white wines. Bentonite can be added at different stages during winemaking. This study aimed to determine the best time to add bentonite. Methods and Results: Unstable juices were vinified after bentonite fining of juice, or with bentonite added either early or late in fermentation. Different addition rates of bentonite were used and bentonite was either added in one or two additions. Fermentation rates were observed and protein stability and bentonite fining rates of the resultant wines were determined. Conclusions: Adding bentonite during fermentation or fining finished wines was the most efficient option in terms of amounts of bentonite required. Fermenting with bentonite present also may increase fermentation rates. Significance of the Study: Using the least bentonite necessary for heat stability is important for winemakers for quality, cost and environmental reasons. This study describes ways in which bentonite addition rates can be minimised.  相似文献   

18.
19.
Proteinogenic wine fining agents are hidden allergens and could present a risk for consumers with allergies. Therefore, the European Parliament adopted Directive 2003/89/EC amending Directive 2000/13/EC to declare ingredients, contaminations and processing aids that are known to trigger allergic reactions. The Amendment Regulation (EU) 1266/2010 excluded the labelling of wines which are processed with hen’s egg and products thereof until 30 June 2012 to get more scientific findings. After 1 July 2012 wine fining agents have to be declared if above 0.25 mg l–1 (Regulation (EU) 579/2012 in conjunction with article 120 g of Regulation (EU) 1234/2007). The Organisation International de la Vigne et du Vin (OIV) advises this limit of detection (LOD) for potential allergenic residues of proteins. Wine fining agents are processing aids and according to the wine producer’s knowledge will be removed after coagulation by filtration or other production steps. Due to lack of scientific data, residues of fining agents in the final product could not be excluded. In this risk assessment, highly sensitive ELISA methods for ovalbumin of known origin for wine have been developed. The objective was to investigate the presence of allergen residues in wine after certain technological treatments were applied to remove the wine fining agents. For all developed ELISA methods the LODs are in the low µg l–1 range between 5 and 10 µg l–1 fining agent, whereas the LOQ varies between 5 and 80 µg l–1 fining agent. The results of the investigation of well-known wines and fining agents demonstrate that white wines fined with white or ovalbumin from hen’s egg could retain allergens. The use of certain technological procedures during wine processing leads to different results. In white wine, bentonite or sheet filtration followed by sterile filtration lead to wines containing no detectable amounts of ovalbumin. In red wine, especially the final sterile filtration removes the fining agents.  相似文献   

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