Development of a Bioenergetics Model for the Round Goby (Neogobius melanostomus)

The round goby (Neogobius melanostomus) was introduced into the Great Lakes in the 1990s through ballast water transfer. Gobies are potential vectors for material transfer between the benthic and pelagic zones. A bioenergetics model was developed for round gobies to enable us to quantify the flow of energy, contaminants, and nutrients from the benthos to pelagic fishes. Weight- and temperature-dependent coefficients for metabolism and consumption were derived. Food consumption increased with temperature up to 26°C before sharply decreasing, and weight-specific consumption decreased with increasing fish weight (allometric coefficient = −0.256, SE = 0.160). Specific oxygen consumption was inversely related to body mass (allometric coefficient = −0.157, SE = 0.025) and increased exponentially with temperature. Estimated Q₁₀ for respiration was 1.84. Additional parameters were obtained from the literature to describe specific dynamic action, egestion, excretion, and reproductive losses. The bioenergetics model explained growth of round gobies in Lake Erie and in their native range.     

Assessment of Potential Effects of Climate Change on Heavy Lake-Effect Snowstorms Near Lake Erie

The potential effects of future climate change on the frequency of heavy lake-effect snowstorms in the Lake Erie snowbelt were assessed using recent transient simulations from two General Circulation Models (GCMs): the second-generation Hadley Centre (HadCM2) and the first generation Canadian Climate Centre (CGCM1) coupled ocean-atmosphere models. An analysis of historical heavy lake-effect snowstorms identified six weather conditions to be closely related to heavy lake-effect snowstorm occurrence: surface wind speed > 6 m/s, surface wind direction of south southwest to west northwest, surface air temperature in the range of −10°C to 0°C, lake surface to air temperature difference > 7°C, lower tropospheric stability (_{T lake − 850} >15°C), and a highly amplified middle tropospheric wave train. These criteria were applied to daily grid point data from the GCMs for two periods, the late 20^th Century and the late 21^st Century, to determine the relative frequency with which heavy lake-effect conditions were predicted. Surface conditions favorable for heavy lake-effect snow decreased in frequency by 50% and 90% for the HadCM2 and CGCM1, respectively, by the late 21^st Century. This reduction was due almost entirely to a decrease in the number of occurrences of surface air temperature in the range of −10 to 0°C, which in turn was the result of an increase in average winter air temperatures. Other surface conditions favorable for lake-effect snow occurred at about the same frequency in the late 21^st Century as in the late 20^th Century, suggesting that lake-effect rain events may replace lake-effect snow events. Changes in the middle tropospheric wave train were also noted in both models. However, there were sizable biases in the simulation of the present-day climate, raising questions about the validity of the future projections.     

Dissolved Organic Matter in the Great Lakes: Role and Nature of Allochthonous Material

Dissolved organic matter (DOM) quality and the modifying influence of light on DOM bioavailability were investigated along a natural gradient of allochthonous influence in the lower Great Lakes. Using parallel factor analysis (PARAFAC), three DOM fluorophores were identified. One fluorophore, previously identified as peak C, was of allochthonous (component 1) origin and two previously uncharacterized fluorophores were identified as autochthonous (components 2 and 3). Component 1 was photoreactive and the dominant form in creek water samples while components 2 and 3 were dominant in Hamilton Harbour and lake water samples. Components 2 and 3 showed limited photoreactivity. Exposure to full spectrum irradiance decreased the average molecular weight of DOM (i.e., increased the absorbance ratio (a254:a365)) for all water samples. DOM bioavailability was lowest in creek and highest in lake water samples and was inversely related to DOM average molecular weight. Photomodification of DOM resulted in higher bacterial activity although these differences were not significantly different. This suggests that light plays a significant role in the cycling of terrestrially-derived DOM and to a certain extent autochthonous DOM, potentially increasing metabolism of both terrestrially and microbially derived DOM in the Great Lakes aquatic ecosystems.     

A Comparison of Photosynthate Allocation in Lakes

We compared the relationships between photosynthate allocation to protein, carbohydrate, lipid and low molecular weight (LMW) fractions and the variables daylength and water temperature in Lakes Huron, Michigan, and Ontario as well as three smaller headwater lakes in the Lake Ontario drainage. In all lakes investigated the allocation of recently produced photosynthate to carbohydrate was strongly related to daylength (% carbohydrate = −3.5 * daylength (hr) + 72.8; n = 59, r² = 0.56). The percentage of photosynthate allocated to protein was a function of water temperature in all lakes although the y-intercept for the protein-temperature relationship was much lower in the three headwater lakes and Lake Ontario (% protein = 0.50 * temperature (°C) + 6.1; n = 37, r² = 0.52) than in Lake Huron and Lake Michigan (% protein = 0.68 * temperature (°C) + 24.2; n = 23, r² = 0.49). The increase in allocation to protein was related to a decrease in allocation to low molecular weight material (% LMW = −1.1 * % protein + 57.13; n = 60, r² = 0.72). The percentages of photosynthate in lipid and LMW material were not related to any of the environmental variables measured. Assuming that photosynthate allocation is related to biochemical composition, the phytoplankton in Lakes Huron and Michigan were more protein rich for a given temperature than those in Lake Ontario and in the smaller inland lakes. The protein deficit was due to an increase in allocation to LMW material.     

Measurement of Diporeia respiration rate for Lake Superior

The freshwater amphipod Diporeia is a dominant macroinvertebrate species in Lake Superior’s benthic community and an important prey item for many fish. A capacity to predict growth and production rates of Diporeia using a bioenergetics model requires information on physiological processes of the species. The objective of this study is to quantify oxygen consumption of Lake Superior Diporeia and to determine if respiration rate changes with body length. Diporeia were collected from Lake Superior and kept over natural sediment maintained at 4 °C. Dissolved oxygen levels for groups of immature (2 mm), juvenile (4 mm), and adult (6 mm) Diporeia in 20 ml microcosms were measured using a polarographic microelectrode. Mass-specific respiration rates for Lake Superior Diporeia ranged from 32.0 to 44.7 mg O₂ g DW^− 1 day ⁻¹. A significant relationship between body length and mass-specific respiration rate (p > 0.1) was not found. The estimate of Diporeia respiration presented here is significantly higher (p < 0.05) than previous findings from populations in Lakes Michigan and Ontario. This study provides new data on respiration rates of Lake Superior Diporeia and compares findings to studies for other connecting Great Lakes.     

Native rainbow smelt and nonnative alewife distribution related to temperature and light gradients in Lake Champlain

Alewife (Alosa pseudoharengus) recently became established in Lake Champlain and may compete with native rainbow smelt (Osmerus mordax) for food or consume larval rainbow smelt. The strength of this effect depends partly on the spatial and temporal overlap of different age groups of the two species; therefore, we need a better understanding of factors affecting alewife and rainbow smelt distributions in Lake Champlain. We used hydroacoustics, trawls, and gill nets to document vertical fish distribution, and recorded environmental data during 16 day–night surveys over two years. Temperature, temperature change, and light were all predictors of adult and age-0 rainbow smelt distribution, and temperature and light were predictors of age-0 alewives' distribution (based on GAMM models evaluated with AIC). Adult alewives were 5–30 m shallower and age-0 alewives were 2–15 m shallower than their rainbow smelt counterparts. Adult rainbow smelt distribution overlapped with age-0 rainbow smelt and age-0 alewives near the thermocline (10–25 m), whereas adult alewives were shallower (0–6 m) and overlapped with age-0 alewives and rainbow smelt in the epilimnion. Adult rainbow smelt were in water < 10–12 °C, whereas age-0 rainbow smelt were in 10–20 °C, and adult and age-0 alewives were in 15–22 °C water. Predicting these species distributions is necessary for quantifying the strength of predatory and competitive interactions between alewife and rainbow smelt, as well as between alewife and other fish species in Lake Champlain.     

Space-for-time proxy for climate change in deep lakes in the Canadian cordillera: Seasonality along a latitudinal climate gradient

A comparison of the annual thermal histories in seven deep, intermontane lakes in western Canada along a north–south transect from 49.67° to 61.25° N reveals latitudinal trends in thermal structure; specifically: 1) summer maximum and winter minimum mean temperatures decrease ~  0.2°C per degree latitude, 2) the dates of maximum and minimum heat content lag ~ 1 and ~  6 days per degree latitude, respectively, 3) the lengths of summer and winter stratification lag ~  8 days per degree latitude, 4) the timing of the spring and autumn turnovers lags ~  4 days per degree latitude, 5) the timing of the onset and break-up of ice cover lags ~ 6 days per degree latitude, and 6) the rate of response to atmospheric heating and cooling is ~  1MJ m^− 2 day^− 1 per degree latitude. We propose that insight into the response of a lake at a specific latitude to climate change can be acquired by examining the present conditions of a morphologically and dynamically similar lake at an appropriate latitude and altitude further south. Of interest is the potential transition of a lake from one mictic state to another, with consequences to the resupply of nutrients to the euphotic zone and to the phenology of spring and fall phytoplankton blooms. The absence or presence of ice cover and timing of freeze-up and break-up also affect a lake's heat budget and thermal history. The full interpretation of climate signals in lake systems requires an understanding of lake-specific physical forcing and response mechanisms.     

Estimation of Zooplankton Mean Length for Use in an Index of Fish Community Structure and its Application to Lake Erie

Mills et al. (1987) developed an index of zooplankton mean size to assess the state of fish communities. The use of this index was evaluated in an assessment of the fish community structure in 1993 at nearshore and offshore sites in the three Lake Erie basins. Mills et al.’s index was developed using a 153-μm mesh net, while the samples in this study have been collected with 64-μm and 110-μm mesh size nets. Two methods were used to convert the data to 153-μm equivalent collections: (a) regression relationships based on simultaneous collections with three mesh sizes, and (b) elimination of smaller organisms that would have passed through the 153-μm mesh by determining the minimum length of inclusion (MLI). The regressions employed for the conversion of zooplankton mean length (ZML) between the nets were: ZML₁₅₃ = 0.137 + 0.988 ZML₁₁₀ (mm) (r²= 0.804) (n = 10) and ZML₁₅₃ = 0.042 + 1.330 ZML₆₄ (mm) (r² = 0.931) (n = 9). The MLI that resulted in the same mean length as the 153-μm sample averaged (± 1 SE) 0.267 ± 0.016 mm (n =19).The comparison between zooplankton mean length and fish community structure in the western basin of Lake Erie in 1993 showed good agreement with Mills et al.’s index. However, the same was not true for the 1988 to 1990 data. Reasons for this discrepancy are discussed.     

Monitoring of COD as an organic indicator in waste water and treated effluent by fluorescence excitation-emission (FEEM) matrix characterization.

The fluorescence excitation-emission matrices (FEEM) of domestic waste water, treated effluent of a waste water treatment plant and receiving river water were analyzed to select wavelengths for the monitoring of organic contents as COD. Excitation/emission wavelengths of 220/350 nm and 270/350 nm for protein-like fluorescence and 240/450 nm and 340/450 nm for humic-like fluorescence were suggested as fluorescence peak emitting wavelength pairs, respectively. Without any pre-treatment, the protein-like fluorescence peaks showed better correlation between COD values and fluorescence intensities than the humic-like fluorescence peaks. No enhanced correlation was observed by removing the suspended solids from the samples using filtration. However, statistical multiple regression methods, using the fluorescence intensities from each peak and the light scattering intensity at 633 nm as variables, resulted in an enhanced correlation, with r2 > 0.9 for the measured and predicted COD values.     

Warmer waters increase the larval sea lamprey's (Petromyzon marinus) tolerance to the lampricide 3-trifluoromethyl-4-nitrophenol (TFM)

Invasive sea lampreys (Petromyzon marinus) in the Great Lakes are controlled by applying the pesticide (lampricide) 3-trifluoromethyl-4-nitrophenol (TFM) to waters infested with larval lamprey. However, treatment effectiveness can be undermined by “residual” larval sea lamprey that survive TFM exposure, and subsequently complete metamorphosis into parasitic juvenile sea lamprey that prey on culturally and economically important fishes. We investigated how season and temperature influenced the TFM tolerance of larval sea lamprey. Acute toxicity tests on lamprey collected from the Au Sable River, Michigan, revealed that the 12-h LC₅₀ and LC_99.9 were 2.0- to 2.5-fold greater in late spring and summer, than in early spring and fall. Subsequent toxicity tests indicated that greater TFM tolerance in summer was due to warmer temperatures, based on an almost 2-fold greater 12-h LC₅₀ and LC_99.9 in warm (24 °C) compared to cool (6 °C) water. Variations in energy stores (glycogen, lipid, protein) or condition did not appear to affect TFM sensitivity. We conclude that higher water temperature is the primary factor driving the larval sea lamprey's greater tolerance to TFM during the summer, possibly due to an increase in their capacity to detoxify TFM. Considering seasonal variations in temperature may be prudent when selecting and treating sea lamprey infested streams with TFM to minimize treatment residuals. In the longer term, increases in average and peak water temperatures due to climate change could result in greater TFM requirements and costs due to the greater tolerance of larval sea lamprey to TFM at warmer temperatures.     

Evidence supporting the importance of nutrient regeneration by nano- and micrograzers for phytoplankton photosynthesis in Lake Malawi/Nyasa

Photosynthesis and nutrient status measurements were compared in size fractioned water samples collected in southern Lake Malawi in three different seasons. The size fractions analyzed were 0–2.0 μm, 0–20 μm, and 0–200 μm. Total chlorophyll concentrations were relatively invariant at about 1 μg L^?1 in all seasons (wet stratified, deep mixing, dry stratified). Over 90% of total chlorophyll was < 20 μm and 40–50% < 2 μm. Stoichiometric ratios of carbon (C):nitrogen (N) and C:phosphorus (P) for the < 2-μm seston were similar to < 20-μm and < 200-μm seston samples and indicated that all size fractions were moderately N and P deficient in all seasons. N and P uptake experiments demonstrated that when the < 2-μm picoplankton were isolated from the larger sizes, they showed high N and P nutrient debts, apparently in response to the removal of nano- and micrograzers. This effect was strongest for N debt in the dry stratified season, but was apparent for P debt in all seasons. The chlorophyll-normalized light saturated rate of photosynthesis, P^b_m, was always higher in whole water samples compared to the rates of the isolated picophytoplankton which were lowest in the dry season. We infer that nano- and micrograzers were an especially important source of regenerated nutrients when the lake was stratified although their removal as a source of nutrient to the picophytoplankton may affect P^b_m in all seasons. Nitrogen regeneration was especially critical to the picophytoplankton in the dry stratified season but less important in the windy deep mixing season.     

Diets of Siscowet Lake Trout from the Apostle Islands Region of Lake Superior, 1993

Stomach contents of 384 siscowet lake trout (Salvelinus namaycush siscowet) from the Apostle Islands region of Lake Superior were examined to determine if diets exhibited seasonal trends or shifted with predator size. The information was also used to qualitatively compare siscowet and lean lake trout (S. namaycush) diets. Stomachs from siscowet less than 500-mm total length (TL) contained primarily macroinvertebrates (clams, Mysis relicta, and insects), sculpins (Cottidae; mean TL = 45 mm), and coregonines (Coregonus spp.; mean TL = 120 mm). Stomachs from siscowet between 500- and 699-mm TL contained items similar to smaller siscowet, but burbot (Lota lota; mean TL = 162 mm and a 254-mm siscowet were also present. Stomachs from siscowet longer than 699-mm TL contained fishes such as burbot (mean TL = 246 mm) and coregonines (mean TL = 240 mm). Dietary proportions of coregonines increased significantly with increasing siscowet length groups. The proportion of the total number of identified taxa and frequency of occurrence of terrestrial insects and sculpins declined significantly across all length groups between June and December. Siscowet and lean lake trout diets were not found to be substantially different and a potential for competition exists.     

Precision of Ages Determined from Scales and Opercles for Yellow Perch Perca flavescens

Scales and opercles were used to age yellow perch Perca flavescens collected in 1989 from Lake Madison (South Dakota), Dauphin Lake (Manitoba), and southern Lake Michigan (Indiana). Three readers aged fish from Lake Madison and Dauphin Lake once and two readers aged fish from Lake Michigan twice. The coefficient of variation (CV) was calculated to compare precision. Ages determined from opercles were as precise as those from scales for fish from Lake Madison (CV = 0 for both structures), and more precise than ages from scales for fish from Dauphin Lake (CV_opercle = 14.0, CV_scale = 27.4, p < 0.001) and Lake Michigan (CV _opercle = 10.6, CV_scale = 13.9, p < 0.001). The high precision of scale and opercle ages for yellow perch from Lake Madison can be attributed to the fast growth rate of fish from that lake and also that only age 1 and 2 fish were aged. The greater precision of opercle ages in comparison to scale ages for Dauphin Lake and Lake Michigan yellow perch can be attributed to ease of recognition of false annuli on opercles as well as to difficulty in distinguishing between false and true annuli crowded on the edge of scales from mature, slower growing fish. Because true annuli are more easily recognized on opercles, ages determined from opercles may be more accurate than ages determined from scales for yellow perch growing at slow or moderate rates.     

Effects of lake-basin morphological and hydrological characteristics on the eutrophication of shallow lakes in eastern China

The Yangtze River floodplain contains numerous oxbow or riverine lakes, all of which were openly connected with the Yangtze River or its major tributaries prior to 1950s. However, stresses resulting from human settlement and utilization of catchment resources have exerted great pressures on these lake ecosystems changing their morphology, connectivity and trophic state lakes. This study examined the interaction of these changes and their impact on eutrophication for 90 shallow lakes in eastern China in 2008 to 2011. TN and TP in the study lakes had negative relationships with mean water depth (Z_mean), but no single lake-basin characteristic was found to dominate chlorophyll-a (Chl-a) growth. Instead, water depth and surface area were found to interactively affect Chl-a concentrations in smaller lakes. That is, Chl-a concentration in the lakes with Z_mean > 2 m and surface area (SA) ≤ 25 km² was significantly higher than that in relatively larger lakes with Z_mean > 2 m and SA > 25 km² (p-value ≤ 0.038). Chl-a concentration was higher in the lakes located within the lower Yangtze River basin which had longer retention times, than in the lakes located within the middle Yangtze River basin, where flow velocity is relatively larger. As expected, the water quality was found to be better in the lakes hydraulically connected with rivers than in those isolated from the river. This study revealed that lake-basin morphology and hydrology dominated algal blooms in the highly eutrophic shallow lakes in eastern China.     

Salmonid Spawning Runs and Estimated Ova Production in Normandale Creek of Lake Erie

The Normandale Creek study area (2,531 m²)provides spawning and nursery grounds for lake-run coho salmon (Oncorhynchus kisutch), rainbow trout (Salmo gairdneri), and brown trout (Salmo trutta), In 1973–74, 59 adult salmonids (58% rainbow trout, 39% coho salmon, and 3% brown trout) ascended the stream between 31 October and 12 May. Upstream movements were significantly related to peak stream discharge (r = 0.21) and highly significantly related to discharge occurring on the day following the peak freshet (r = 0.34). Despite a highly significant correlation (r = 0.29) between flow and water temperature, the latter factor is not significantly related with upstream movement of adult fish. Coho salmon spawned at water temperatures of 1 to 10° C between 2 November and 19 December, rainbow trout at 1 to 15° C between 9 November and 14 May, and brown trout at 7° C between 4 and 5 November. Of 86 nests constructed, 60% were disturbed by re-use or sand deposition. From a calculated deposition of 90,403 ova, it is estimated that some 22% survived to emergence.     

Habitat characteristics of a unionid refuge in the thermal plume of a power plant in western Lake Erie

We examined habitat characteristics to determine why a rich community of unionid mussels lives in the thermal plume of the Bayshore Power Plant, Oregon Ohio. We expected to find that the power plant reduced the density and viability of dreissenid veligers and that the depth and organic matter content of sediments in the thermal outflow were greatest near the plant. Water temperature averaged 3.8 ± 1.5 °C warmer at the plant's outflow than intake, and the ratio of live:dead veligers was significantly lower at the outflow than intake on most dates (P ≤ 0.05). A laboratory experiment confirmed that heating water comparable to passing through the power plant significantly reduced veliger viability (P ≤ 0.01). However, wind direction affected the differences in veliger density between the intake and outflow (P ≤ 0.01), with easterly winds (opposing the direction of discharge) increasing the density of veligers at the outflow. Similarly, water temperature declined with distance from the plant, but east winds increased variations in water temperatures. Apparently wind direction facilitates (westerly) or slows (easterly) the discharge of water from the small embayment receiving the power plant's thermal effluent. Particulate organic matter content of the thermal plume varied with wind speed, apparently due to suspension of sediments in this shallow water. Finally, only the coarse (> 10 mm) size fraction of the benthic sediments was related to distance from the power plant. Thus the thermal regime of this habitat appears to be the primary explanation for this unionid refuge.     

Relationships Between Chlorinated Hydrocarbon Concentrations and Rearing Mortality of Chinook Salmon (Oncorhynchus Tshawytscha) Eggs from Lake Michigan

A study was conducted to investigate a possible correlation between chlorinated hydrocarbon concentrations and rearing mortality of chinook salmon. Eggs, collected from adult Lake Michigan chinook salmon in October, 1982, were analyzed for chlorinated hydrocarbon concentrations and rearing mortality. Polychlorinated biphenyls, toxaphene, and fifteen other chlorinated hydrocarbons were quantified. Also, concentrations of individual PCB congeners were quantified. We found 78 quantifiable PCB congeners, only three of which were non-ortho substituted. Mean concentrations of Arocloi^® 1242, 1254 and 1260 were 1.7, 5.4, and 1.1 μg/g, respectively. The concentration of toxaphene-like material was 3.3 μg/g, and the mean concentration of the total DDT complex was 1.0 μg/g. Concentrations of chlorinated hydrocarbons ranged approximately 10-fold among individual fish. An analysis of variance components demonstrated that this variability represented among-fish variation and was not due to errors in sampling or quantification. Duplicate studies of egg viability and fry mortality, conducted at 10, 11, and 15°C, correlated with residue concentrations. While some residue concentrations were significantly negatively correlated with egg viability and survival of fry, others were positively correlated. Thus, we observed no consistent trends in Pearson, pairwise-correlations between either egg viability or survival of fry to swim-up stage and individual residue concentrations. When the residues were classified into four principal components, two of the principal components which contained primarily the toxaphene and PCB concentrations were negatively correlated with survival of fry to the swim-up stage.     

Accumulation of Contaminant Metals in the Amphipod Diporeia spp. in Western Lake Ontario

Sediment and amphipods (Diporeia spp.) were collected in areas in western Lake Ontario suspected of containing a range of sediment metal contents. The total metal contents (Al, As, Cd, Cr, Cu, Ni, Fe, V, and Zn) of depurated Diporeia tissues were measured and compared to the total metal contents of the surrounding sediment. The fractional bioavailability of As, Cu, Fe, and Zn was determined in those same sediments using a sequential chemical extraction (SCE) procedure and correlated with depurated Diporeia tissue metal contents. Results of these analyses were then used to assess the ability of Diporeia to accumulate sediment metal contamination. A comparison of metal biota-sediment accumulation factors (BSAFs) and sediment metal enrichment factors (EFs) for each metal yielded separate metal groups showing different behavior for tissue accumulation. Aluminum, Cr, Fe, Ni, and V were not enriched in the sediments and were not accumulated in Diporeia tissues, while As, Cd, Cu, and Zn were enriched in the sediments and accumulated in Diporeia tissues. SCE results showed that Cu (3.2 to 8.4 %) and Zn (13 to 19 %) contents in the easily extractable sediment fractions (MgCl₂ and Na-acetate) were significantly correlated with Cu (r = 0.86; P<0.01) and Zn (r = 0.69; P<0.05) contents in depurated Diporeia tissue. In addition, Cu and Zn in Diporeia tissues are also correlated to the total sediment metal content for each respective metal (Cu: r = 0.67, P < 0.05; Zn: r = 0.66, P < 0.05). The high BSAFs for Cu and Zn, in combination with the association of tissue metal contents with easily extracted sediment metal fractions, suggest that Diporeia may be a potential bioindicator for Cu and Zn contamination in sediments.     

Sensitivity of Ancient Lake Ohrid to Local Anthropogenic Impacts and Global Warming

Human impacts on the few ancient lakes of the world must be assessed, as any change can lead to an irreversible loss of endemic communities. In such an assessment, the sensitivity of Lake Ohrid (Macedonia/Albania; surface area A = 358 km², volume V = 55 km³, > 200 endemic species) to three major human impacts—water abstraction, eutrophication, and global warming—is evaluated.It is shown that ongoing eutrophication presents the major threat to this unique lake system, even under the conservative assumption of an increase in phosphorus (P) concentration from the current 4.5 to a potential future 9 mg P m⁻³. Eutrophication would lead to a significant reduction in light penetration, which is a prerequisite for endemic, deep living plankton communities. Moreover, a P increase to 9 mg P m⁻³ would create deep water anoxia through elevated oxygen consumption and increase in the water column stability due to more mineralization of organic material. Such anoxic conditions would severely threaten the endemic bottom fauna. The trend toward anoxia is further amplified by the predicted global warming of 0.04°C yr^–1, which significantly reduces the frequency of complete seasonal deep convective mixing compared to the current warming of 0.006°C yr⁻¹. This reduction in deep water exchange is triggered by the warming process rather than by overall higher temperatures in the lake. In contrast, deep convective mixing would be even more frequent than today under a higher temperature equilibrium, as a result of the temperature dependence of the thermal expansivity of water. Although water abstraction may change local habitats, e.g., karst spring areas, its effects on overall lake properties was shown to be of minor importance.