共查询到20条相似文献,搜索用时 15 毫秒
1.
《Dyes and Pigments》2012,92(3):350-355
A novel fluorescent sensor based on thiooxorhodamine B has been prepared to detect Hg2+ in aqueous buffer solution. It demonstrates high selectivity for sensing Hg2+ with about 383-fold enhancement in fluorescence emission intensity and micromolar sensitivity (Kd = 7.5 × 10−6 mol L−1) in comparison with alkali and alkaline earth metal ions (K+, Na+, Mg2+, Ca2+) and other transition metal ions (Mn2+, Ni2+, Co2+, Cu2+, Zn2+, Cd2+, Ag+, Pb2+, Cr3+, Fe3+). Meanwhile the distinct color changes and rapid switch-on fluorescence also provide ‘naked eyes’ detection for Hg2+ over a broad pH range. Moreover, such sensor is cell-permeable and can visualize the changes of intracellular mercury ions in living cells using fluorescence microscopy. 相似文献
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A chemosensor based on rhodamine B with 1, 2, 4-triazole as subunit was synthesized and characterized. It exhibits high selectivity and sensitivity for Cu2+ in ethanol/water (6:4, v:v) of pH 7.0 HEPES buffer solution and undergoes ring opening mechanism, and a 2:1 metal-ligand complex is formed. The chemosensor displays a linear response to Cu2+ in the range between 1.0 × 10−7 M and 1.0 × 10−6 M with a detection limit of 4.5 × 10−8 M. Its capability of biological application was also evaluated and the results show that this chemosensor could be successfully employed as a Cu2+-selective chemosensor in the fluorescence imaging of living cells. 相似文献
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Yunlong LiuXin Lv Yun ZhaoMaliang Chen Jing LiuPi Wang Wei Guo 《Dyes and Pigments》2012,92(3):909-915
On the basis of fluorescent resonance energy transfer from 1,8-naphthalimide to rhodamine B, a new fluorophore dyads (4) containing rhodamine B and a naphthalimide moiety was synthesized as a ratiometric fluorescent probe for detecting Hg2+ with a broad pH range 5.7-11.0. The selective fluorescence response of 4 to Hg2+ is due to the Hg2+-promoted desulfurization of the thiocarbonyl moiety, leading to the ring-opening of rhodamine B moiety of 4. When 4 was employed at 0.1 μM with the slit size being 20 nm/20 nm, a low level of Hg2+ (up to 3 × 10−8 M) can be detected using the system. 相似文献
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The conjugated polymer P-1 could be synthesized by the polymerization of 4,7-diethynyl-benzo[2,1,3]thiadiazole (M-1) and 1,4-bis[3′-(N,N-diethylamino)-1′-oxapropyl]-2,5-diiodobenzene (M-2) via Pd-catalyzed Sonogashira reaction. The water-soluble conjugated polyelectrolyte P-2 could be obtained by the reaction of P-1 with ethyl bromide. Both P-1 and P-2 can emit orange fluorescence. The responsive optical properties of P-1 and P-2 on Hg2+ were investigated by fluorescence spectra. Hg2+ can lead to nearly complete fluorescence quenching of P-1. On the contrary, Hg2+ can show the most pronounced fluorescence enhancement response of P-2 in aqueous solution without interference from those coexistent ions, such as K+, Mg2+, Pb2+, Co2+, Ni2+, Ag+, Cd2+, Cu2+, Fe3+ and Zn2+. The results also exhibit that this kind of water-soluble conjugated polyelectrolyte can be used as a highly sensitive and selective fluorescence sensor for Hg2+ detection in water. 相似文献
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Can Wang Chenchen Bao Shujing Liang Lingxia Zhang Hualin Fu Yutian Wang Kan Wang Chao Li Min Deng Qiande Liao Jian Ni Daxiang Cui 《Nanoscale research letters》2014,9(1):274
The successful development of safe and highly effective nanoprobes for targeted imaging and simultaneous therapy of in vivo gastric cancer is a great challenge. Herein we reported for the first time that anti-α-subunit of ATP synthase antibody, HAI-178 monoclonal antibody-conjugated fluorescent magnetic nanoparticles, was successfully used for targeted imaging and simultaneous therapy of in vivo gastric cancer. A total of 172 specimens of gastric cancer tissues were collected, and the expression of α-subunit of ATP synthase in gastric cancer tissues was investigated by immunohistochemistry method. Fluorescent magnetic nanoparticles were prepared and conjugated with HAI-178 monoclonal antibody, and the resultant HAI-178 antibody-conjugated fluorescent magnetic nanoparticles (HAI-178-FMNPs) were co-incubated with gastric cancer MGC803 cells and gastric mucous GES-1 cells. Gastric cancer-bearing nude mice models were established, were injected with prepared HAI-178-FMNPs via tail vein, and were imaged by magnetic resonance imaging and small animal fluorescent imaging system. The results showed that the α-subunit of ATP synthase exhibited high expression in 94.7% of the gastric cancer tissues. The prepared HAI-178-FMNPs could target actively MGC803 cells, realized fluorescent imaging and magnetic resonance imaging of in vivo gastric cancer, and actively inhibited growth of gastric cancer cells. In conclusion, HAI-178 antibody-conjugated fluorescent magnetic nanoparticles have a great potential in applications such as targeted imaging and simultaneous therapy of in vivo early gastric cancer cells in the near future. 相似文献
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The multilayered thin films composed diazoresin (DR) and brilliant yellow (BY) were fabricated by a layer-by-layer (LbL) deposition technique, and the ionic bonds between diazonium ion in DR and sulfonate residues in BY were converted to covalent bonds by UV light irradiation. The cross-linking between BY and DR prevented BY from desorption even in a pH 13 solution. The cross-linked (DR/BY)10DR film exhibited pH-dependent absorption spectra in pH 9-13, and the response was repeatable and quick. The pH response time of the films was within a few seconds upon change from pH 9.0 to 13.0, and ca. 40 s upon change from pH 13.0 to 9.0. 相似文献
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Zhanxian Li Lifeng ZhangXiaoya Li Yongkai GuoZhonghai Ni Jianhong ChenLiuhe Wei Mingming Yu 《Dyes and Pigments》2012,94(1):60-65
A fluorescent color/intensity changed fluoranthene derivative chemosensor for Fe3+ has been prepared and confirmed by 1H-NMR, 13C-NMR, HRMS, and crystal data, which displays a high selectivity and antidisturbance for Fe3+ among environmentally and biologically relevant metal ions. Fluorescence studies show that fluorescent emission peak blue shifts about 100 nm with fluorescent intensity enhancing 75-fold, indicating a Fe3+-selective dual-emission behavior. Further study demonstrates the detection limit on fluorescence response of the sensor to Fe3+ is down to 10−7 M range. The fluorescence signals of chemosensor can be restored with o-phenanthroline, showing the binding of chemosensor and Fe3+ is really chemically reversible. 相似文献
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Mingyue Zhu Zhenhao Tian Lingling Jin Xiaokui Huo Chao Wang Jingnan Cui Yan Tian Xiangge Tian Lei Feng 《Frontiers of Chemical Science and Engineering》2022,16(1):103-111
Uridine diphosphate(UDP)-glucuronosyltransferases(UGTs)are enzymes involved in the biotransformation of important endogenous compounds such as steroids,bile acids,and hormones as well as exogenous substances including drugs,environmental toxicants,and carcinogens.Here,a novel fluorescent probe BDMP was developed based on boron-dipyrromethene(BODIPY)with high sensitivity for the detection of UGT1A8.The glucuronidation of BDMP not only exhibited a redemission wavelength(lex/lem=500/580 nm),but also displayed an excellent UGT1A8-dependent fluorescence signal with a good linear relationship with UGT1A8 concentration.Based on this perfect biocompatibility and cell permeability,BDMP was successfully used to image endogenous UGT1A8 in human cancer cell lines(LoVo and HCT15)in real time.In addition,BDMP could also be used to visualize UGT1A8 in tumor tissues.These results suggested that BDMP is a promising molecular tool for the investigation of UGT1A8-mediated physiological function in humans. 相似文献
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Wei Y Aydin Z Zhang Y Liu Z Guo M 《Chembiochem : a European journal of chemical biology》2012,13(11):1569-1573
An eye for an iron: A highly sensitive, selective and reversible turn-on Fe(3+) sensor for imaging labile Fe(3+) in live cells at subcellular resolution is reported. The sensor can respond to changes in intracellular Fe(3+) levels and was used to image endogenous chelatable Fe(3+) in live human neuroblastoma SH-SY5Y cells, with two Fe(3+) pools being identified in mitochondria and endosomes/ lysosomes for the first time. 相似文献
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We consider the steady-state pattern of messenger molecules produced in the membrane of a cell perceiving and responding to an extracellular gradient of chemoattractant, which directs cell movement towards the chemoattractant source. Specifically, we analyze the undesirable effect of lateral diffusion in blurring the intracellular messenger profile. The concept of an effectiveness factor, akin to the analysis of reactions in porous catalysts, is applied to the spatial gradient sensing problem, with the distinction that slow, not fast, diffusion is required for effective gradient sensing. Analytical effectiveness factor expressions are derived for ideal geometries and then generalized to arbitrary cell shapes. In the case of mouse fibroblasts responding to gradients of platelet-derived growth factor, we conclude that the cell morphology and orientation with respect to the gradient can dictate whether messenger diffusion obliterates gradient sensing or has very little effect. The analysis outlined here allows the effect of intracellular messenger diffusion on spatial gradient sensing to be quantified for individual cells. 相似文献
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A new 4-amino-1,8-naphthalimide-based fluorescent sensor with iminodiacetic acid as receptor, was synthesized and characterized. Under physiological pH conditions, it demonstrates high selectivity and sensitivity for sensing Zn2 + with about 50-fold enhancement in fluorescence intensity. The fluorescent sensor exhibited a characteristic emission band of 4-amino-1,8-naphthalimide with a green color centered at ~ 550 nm and was successfully applied to image Zn2 + in living cells. Upon sensing of Zn2 + the fluorescence emission spectrum is “switched on” demonstrating the suppression of PET from the receptor to the fluorophore. 相似文献
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Li Yang Mengqi Wang Xiaoyu Gu Wei Zhang Ping Li Wen Zhang Hui Wang Bo Tang 《Frontiers of Chemical Science and Engineering》2023,17(3):298
Herein, a reversible pH fluorescent sensor was developed using caffeic acid as the precursor by one-step solvothermal synthesis method. The carbon dots-based sensor (CA-CDs) exhibited pH-dependent increase in fluorescence intensity and showed linear relationship in the range of pH 6.60 and 8.00. Notably, the fluorescence sensor has a reversible response to pH change. Finally, the CA-CDs has been successfully applied for two-photon imaging of the pH in liver and kidney of diabetic mice. Imaging results showed that the pH value in kidney of diabetic mice was lower than that of the normal mice, while the pH value in liver of diabetic mice was almost the same as that of the normal mice. The present study provides a simple analytical method for pH detection suitable for in vivo. 相似文献
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Harrison D. Root Gregory Thiabaud Jonathan L. Sessler 《Frontiers of Chemical Science and Engineering》2020,14(1):19
We report here a water-soluble metal cation sensor system based on the as-prepared or reduced form of an expanded porphyrin, texaphyrin. Upon metal complexation, a change in the redox state of the ligand occurs that is accompanied by a color change from red to green. Although long employed for synthesis in organic media, we have now found that this complexation-driven redox behavior may be used to achieve the naked eye detectable colorimetric sensing of several number of less-common metal ions in aqueous media. Exposure to In(III), Hg(II), Cd(II), Mn(II), Bi(III), Co(II), and Pb(II) cations leads to a colorimetric response within 10 min. This process is selective for Hg(II) under conditions of competitive analysis. Furthermore, among the subset of response-producing cations, In(III) proved unique in giving rise to a ratiometric change in the ligand-based fluorescence features, including an overall increase in intensity. The cation selectivity observed in aqueous media stands in contrast to what is seen in organic solvents, where a wide range of texaphyrin metal complexes may be prepared. The formation of metal cation complexes under the present aqueous conditions was confirmed by reversed phase high-performance liquid chromatography, ultra-violet-visible absorption and fluorescence spectroscopies, and high-resolution mass spectrometry. 相似文献
15.
Marcos F. S. Teixeira Carla C. S. Cavalheiro Luiz A. Ramos Eduardo Almeida Neves 《Applied Clay Science》2003,23(5-6):323-328
The construction and analytical evaluation of a pH sensor based in a matrix containing 20% of [(Mg6Si8O20)](OH)4, a magnesium silicate, talc and 30% graphite dispersed in an epoxy resin (50%) is described. The data obtained from various acid–base titrations were compared with those using a glass electrode in the same conditions. The proposed electrode presented a linear response in the 1–12 pH range with a slope of −39.9±0.3 mV/pH (at 25 °C) and ca. 15 s of response time. The lifetime of this electrode was higher than 8 months (ca. 1500 determinations) and it showed a good performance for pH determination and end-point indication in the potentiometric titrations of acids and bases. 相似文献
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Narendra Reddy ChereddySathiah Thennarasu 《Dyes and Pigments》2011,91(3):378-382
A bis-rhodamine based fluorescent chemosensor for naked-eye detection of Cu2+ with enhanced sensitivity as compared to mono-rhodamine derivative has been synthesized, and its selectivity for Cu2+ in the presence of other competitive metal ions (Li+, Na+, K+, Cs+, Mg2+, Ca2+, Sr2+, Cr3+, Mn2+, Fe2+, Fe3+, Co2+, Ni2+, Zn2+, Cd2+, Hg2+, and Pb2+), and application in bio-imaging are demonstrated. 相似文献
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