Characteristics of soluble immune complexes prepared from oligovalent DNP conjugates and anti-DNP antibodies

The stability of soluble immune complexes was investigated after isolation by gel filtration and sucrose gradient ultracentrifugation. Soluble immune complexes were formed between specific goat anti-dinitrophenol (DNP) antibodies and DNP conjugated to a large (19 S) carrier, namely bovine thyroglobulin. The composition and molecular weight of these complexes were determined by ultracentrifugation on calibrated sucrose density gradients and the use of different isotopic markers for antigen and antibody. A good separation of immune complexes containing one, two, or three antigen molecules per complex was obtained by ultracentrifugation while gel filtration was less effective. Ultracentrifugational analysis of fractions isolated by these two procedures showed that large immune complexes containing more than one antigen were relatively labile, whereas small immune complexes containing one antigen were stable. The stability of large immune complexes was dependent on dilution. Because dilution affects the size and composition of soluble immune complexes, it is important to emphasize that for the investigation of a causal relationship between the biological properties and the size and composition of immune complexes, analysis of the immune complexes should be performed in the same dilutions in which they will be used experimentally.     

钍与双希夫碱配合物的合成与表征

由二溴水杨醛缩二胺与硝酸钍合成了两种新的固体配合物,并对合成的配合物进行了元素分析和红外光谱、紫外光谱、差热热重、摩尔电导分析,确定了配合物的组成均为ＴｈＬ（ＮＯ３）４·Ｈ２Ｏ（Ｌ为配体）。     

Synthesis, Characterization and Luminescent Properties of Complexes of Rare Earth Nitrates with 2,2′:4′,4″:2″,2-Quaterpyridine

The rare earth nitrate complexes with 2,2′:4′,4″:2″,2-quaterpyridine (L) were prepared in ethylacetate. These new complexes with the general formula [RE(NO3)3L]*H2O (RE = La, Pr, Eu, Tb, Er, Y) were characterized by elemental analysis, IR spectra, thermal analysis and molar conductance measurements. The luminescence properties of these complexes were also studied.     

Ti—6242S合金在β区的锻造工艺研究

采用光学显微镜、扫描电和力学性能测试等设备了Ｔｉ－６２４２Ｓ合金相变点以上４５℃加热,变形量为７０％空冷的锻造工艺,经双重退火处理,获得细小网篮状组织。这种组织为α＋β两相区锻造下的等轴组织相比,不但具有良好的室温和高温力学性能,而且还提高了材料的高温持久、蠕变强度、冲击和断裂韧性,该合金的β区锻造工艺,放于加工航空发动机压气机盘模锻件。     

Highly luminescent europium and terbium complexes based on succinimide and N-hydroxysuccinimide

The preparation of two highly luminescent europium(III) and terbium(III) complexes with succinimide(SI) and N-hydroxysuccinimide(NHSI) were reported,which were further investigated through elemental chemical analysis,thermal analysis,FT-IR,powder X-ray diffraction,SEM and fluorescence spectroscopy.Data collected through these analysis revealed the formation of the Eu(III) and Tb(III) complexes with the above mentioned ligands at a metal to ligand(M:L) molar ratio of 1:3.Interesting conclusions regarding the efficient sensitization through the coordination site of the lanthanide luminescent centers were retained from the photoluminescent spectra investigation.Strong luminescence emission was observed in case of Eu(III)-SI and Tb(III)-NHSI complexes while the corresponding Eu(III)-NHSI and Tb(III)-SI complexes exhibited no photo luminescent properties.Newly obtained luminescent lanthanide complexes may be of particular interest for various applications in optoelectronics.     

几种对称钕卟啉配合物的固相合成及电化学性质

在固相条件下合成了四苯基卟啉钕、四 (对羟基苯基 )卟啉钕、四 (对甲氧基苯基 )卟啉钕、四 (对硝基苯基 )卟啉钕四种钕卟啉配合物。并用元素分析、ICP、U V、IR等手段对配合物进行了表征。用循环伏安法研究了它们的电化学性质。实验结果表明 :四种钕卟啉配合物的电化学性质相似。在循环伏安图上均出现了三对氧化还原电位。但由于卟啉环上对位取代基不同 ,峰电位值不同     

Nd（Ⅲ）、Sm（Ⅲ）、Eu（Ⅲ）与邻香兰素缩邻苯二胺配合物的合成与性质

合成了稀土硝酸盐与双希夫碱邻香兰素缩邻苯二胺（以Ｌ表示）的３种新固体配合物。通过元素分析、红外光谱、紫外光谱、差热一热重、摩尔电导及Ｘ射线粉末衍射分析等手段，确定配合物的组成为（ＬｎＬ（ＮＯ３）２）ＮＯ３（Ｌｎ＝Ｎｄ、Ｓｍ、Ｅｕ）。并对其结构和某些物理化学性质进行了研究。     

Synthesis,characterization, biological activities and luminescent properties of lanthanide complexes with [2-thiophenecarboxylic acid, 2-(2-pyridinylmethylene)hydrazide] Schiff bases ligand

A Schiff base L [2-thiophenecarboxylic acid, 2-(2-pyridinylmethylene)hydrazide] with its lanthanide metal complexes was synthesized. These complexes were characterized by elemental analysis, molar conductivity measurements, spectral analysis(NMR, FT-IR, and UV-Vis), luminescence and thermal gravimetric analysis. The Schiff base ligand was a tridentate chelate and coordinates to the central lanthanide ion with 1:2 metal:ligand ratio. The conductivity data showed a 1:1 electrolytic nature with a general formula [LnL_2(NO_3)_2]NO_3. The luminescence emission properties for Sm, Tb, and Eu complexes were observed and showed that the ligand L could absorb and transfer energy to Sm(III), Tb(III) and Eu(III) ions. The complexes possessed a good antibacterial activity against different bacterial strains. In addition, the scavenging activity of the Ln(III) complexes on DPPH was concentration dependant and the complexes were significantly more efficient in quenching DPPH than the free Schiff base ligand.     

Synthesis, characterization, cytotoxicity, DNA cleavage and antimicrobial activity of homodinuclear lanthanide complexes of phenylthioacetic acid

Lanthanide complexes of Eu(III), Gd(III), Nd(III), Sm(III), and Tb(III) with phenylthioacetic acid were synthesized and characterized by elemental analysis, mass, infrared radiation (IR), electronic sp...     

Sm(Ⅲ),Eu(Ⅲ)-丙烯酸-8-羟基喹啉三元配合物的合成及荧光性质研究

用三异丙氧基钐（铕）与８－羟基喹啉、丙烯酸反应合成具有良好荧光性质的稀土三元配合物,通过元素分析、红外光谱、紫外光谱证实其分子结构,并进行荧光光谱、热分析测试,发现配合物具有较好的荧光性质。     

Th(Ⅳ)和UO2(Ⅱ)与希夫碱的双核配合物的合成与表征

合成了硝酸钍、硝酸铀酰与希夫碱邻香兰素缩邻氨基苯甲酸（Ｈ２Ｌ表示）的两种新双核固体配合物,通过元素分析、红外光谱、紫外光谱、差热－热重及摩尔电导分析等方法,确定配合物的组成分别为「Ｔｈ２（ＨＬ）３（ＮＯ３）２」（ＮＯ３）３、「（ＵＯ２）２（ＨＬ）３」ＮＯ３,并对它们的某些性质进行了研究。     

稀土(Ⅲ)硝酸盐与香兰素缩己二胺配合物的合成与性质

在非水体系中合成了稀土（Ⅲ）硝酸盐（Ｌａ、Ｎｄ、Ｅｒ、Ｙ）与双希夫碱香兰素（４－羟基－３－甲氧基苯甲醛）缩己二胺的固体配合物,用元素分析、摩尔电导、红外光谱、紫外光谱、差热－热重分析等手段研究其组成和性质。     

Synthesis, characterization, fluorescence and DNA-binding studies of europium（Ⅲ） pirates complexes with amide-based 2,3-dihydroxynaphthalene derivatives

Two ligands 2,2′-[2,3-naphthylenebis(oxy)]-bis(N-benzyl(acetamide))(L1) and 2,2′-[2,3-naphthylenebis (oxy)]-bis(N,N-diphenyl (acetamide))(L2) and their europium(Ⅲ) picrate complexes were synthesized. The complexes were characterized by elemental analysis, infra-red (IR), thermogravimetry and differential thermal analysis (TG-DTA) and molar conductivity. Fluorescent experiments showed that the resonance level of the Eu) matched better to the triplet state energy level of the ligand L2 than that of the ligand L1 and the fluorescence in-tensities of the complexes were reduced with the raising coordination ability of solvent. In addition, the interactions between the complexes and DNA were studied by means of spectrometry and cyclic voltammetry. The results suggested that the complexes could bind to DNA through intercalation and the complex 1 binded to DNA more strongly than the complex 2.     

Synthesis, Characterization and Antibacterial Properties of Rare Earth (Ce3+, Pr3+, Nd3+, Sm3+, Er3+)Complexes with L-Aspartic Acid and o-Phenanthroline

Five novel ternary complexes of rare earth ions with L-Aspartic acid (Asp) and o-phenanthroline (Phen) were synthesized in ethanol aqueous solution. Their compositions were characterized by elemental analysis, molar conductance, FT-IR, Raman, UV-VIS and TG-DTA. The compositions of the complexes were confirmed to be: RE(Asp)3PhenCl3·3H2O (RE: Ce3 , Pr3 , Nd3 , Sm3 , Er3 ). The antibacterial activity test shows that all these complexes exhibit excellent antibacterial ability against Escherichia coli, Staphylococcus aureus and Candida albicans. The antimicrobial spectrum of the complexes are broad.     

稀土硝酸盐与希夫碱邻香兰素缩丙氨酸盐及邻菲咯啉三元配合物的合成与表征

合成了稀土硝酸盐与希夫碱邻香兰素缩丙氨酸盐(以KHL表示)及邻菲咯啉的三种固体三元配合物。通过元素分析、差热-热重、红外光谱、紫外光谱及摩尔电导分析等手段,确定配合物的组成为［RE(L)(Phen)(H2O)］NO3,(RE=Y,Nd,Sm)。并对它们的配位方式及某些性质进行了研究。     

含N_2O_2四齿希夫碱配体的稀土有机配合物的合成和表征

室温下 ,采用 Schlenk操作技术 ,用 Cp3L n和对称的希夫碱双水杨醛缩邻苯二胺 ( H2 SAL OPHEN)为原料 ,物质的量之比为 1∶ 1,在四氢呋喃介质中反应 ,合成了六个新型配合物 Cp L n SAL OPHEN( L n=Y,Pr,Sm,Ho,Er,Tm)。所有这些配合物都经过了元素分析、IR和 Ms表征     

含γ-羟丙基四氮大环西佛碱稀土配合物的合成与表征

用3,4-二氢-2H-吡喃-5-醛与邻苯二胺作用合成7,16-二羟丙基-5,14-二氢二苯并[b,i]-[1,4,8,11]四氮杂[14]轮烯(H2L),后者分别与三价镧系氯化物在乙腈中回流,得到相应的大环配合物。通过元素分析、红外光谱、紫外-可见光谱和核磁共振谱、摩尔电导测定对所得大环配合物进行了结构表征。     

Synthesis,fluorescence properties of Tb(Ⅲ) complexes with novel mono-substituted β-diketone ligands

Two novel pyridine-2,6-dicarboxylic acid derivatives of mono-β-diketone named methyl 6-biphenylacetyl-2-pyridinecarboxylate (MBP) and 6-biphenylacetyl-2-pyridinecarboxylic acid (BAA) and their corresponding binary complexes Tb(MBP)3.6H2O and Tb(BAA)3·6H2O were synthesized. The ligands were characterized by elemental analysis, FT-IR and 1H-NMR, and the complexes were characterized with elemental analysis, FT-IR, 1H-NMR and thermogravimetric and differential thermal analysis(TG-DTA). The investigation of fluorescence properties of the complexes Tb(MBP)3·6H2O and Tb(BAA)3·6H2O showed that the introduction of the biphenyl enlarged the π-conjugated system of the ligands and enhanced the luminescent intensity of the complexes. The Tb(Ⅲ) ion could be sensitized more efficiently by the ligands, in particular, the fluorescence intensity of the complex Tb(BAA)3·6H2O was about 20% higher than that of Tb(MBP)3·6H2O and 30% higher than that of Tb(Ⅲ) complexes with bis-β-diketone-type ligands that we have previously reported.     

Synthesis and Application of Lanthanide Complexes with Schiff Base of Pridoxylidence-Glycine

Ｔｈｅｈａｒｍｆｕｌｎｅｓｓｏｆｈｅａｖｙｍｅｔａｌｅｌｅｍｅｎｔｓｏｎｐｌａｎｔｓｉｓｏｎｅｏｆｔｈｅｓｅｒｉｏｕｓｅｎｖｉｒｏｎｍｅｎｔａｌｐｒｏｂｌｅｍｓｏｆｔｈｅｍｏｄｅｒｎｗｏｒｌｄ .Ｉｎｒｅｃｅｎｔｙｅａｒｓ ,ｔｈｅｓｔｕｄｙｏｎｔｈｅｍｉｓａｌｓｏｏｎｅｏｆｖｅｒｙａｃ ｔｉｖｅｒｅｓｅａｒｃｈｐｒｏｊｅｃｔｓｉｎｐｌａｎｔｐｏｌｌｕｔｉｏｎｅｃｏｌｏｇｙｆｉｅｌｄｓ[1～ 6 ] .ＬａｎｔｈａｎｉｄｅｃｏｍｐｌｅｘｅｓｗｉｔｈｔｈｅＳｃｈｉｆｆｂａｓｅａｒｅａｃｌａｓｓｏｆｃｏｍｐｏｕ…     

稀土铕,铽β-二酮配合物的合成与发光性能

采用Claisen缩合反应合成了一种新型的β-二酮化合物1-(4-溴苯)-3-苯基丙烷-1,3-二酮(L),并以其为第一配体,邻菲罗啉(phen)为第二配体,合成出新型稀土铕,铽二元及三元配合物。通过元素分析、红外光谱、紫外光谱、荧光光谱对合成的配体及配合物进行了表征。元素分析确定了配合物的组成。红外光谱的分析表明第一配体L中的氧原子以及第二配体phen中的氮原子与稀土离子进行了配位。紫外光谱表明第一配体L为能量的给体,第二配体phen起协同作用。通过荧光光谱研究了配合物的发光性质,结果显示三元配合物的发光强度大于二元配合物,三元配合物Eu(L)3phen表现出Eu3+的特征发射,在593,615,653,701 nm处的发射峰分别归属于Eu3+的5D0→7Fj(j=1,2,3,4)能级间的跃迁,并且以位于615 nm处的5D0→7F2电子跃迁所发出的荧光强度最大;而铽配合物中并没有出现Tb3+的特征发射。进一步的研究表明,这是由于配体L的最低三重态能级较适合Eu3+的发射能级,配体L吸收的能量可以有效的通过Antenna效应传递给稀土中心离子,使得三元配合物Eu(L)3phen的发光强度较大。