共查询到20条相似文献,搜索用时 15 毫秒
1.
Dispersed nanosphere lithography can be employed to fabricate gold nanostructures for localized surface plasmon resonance, in which the gold film evaporated on the nanospheres is anisotropically dry etched to obtain gold nanostructures. This paper reports that by wet etching of the gold film, various kinds of gold nanostructures can be fabricated in a cost-effective way. The shape of the nanostructures is predicted by profile simulation, and the localized surface plasmon resonance spectrum is observed to be shifting its extinction peak with the etching time. 相似文献
2.
Shuhei Uchida Nobuyuki Zettsu Katsuyoshi Endo Kazuya Yamamura 《Nanoscale research letters》2013,8(1):274
In this work, we focused on the label-free detection of simple protein binding using near-infrared light-responsive plasmonic nanoshell arrays with a controlled interparticle distance. The nanoshell arrays were fabricated by a combination of colloidal self-assembly and subsequent isotropic helium plasma etching under atmospheric pressure. The diameter, interparticle distance, and shape of nanoshells can be tuned with nanometric accuracy by changing the experimental conditions. The Au, Ag, and Cu nanoshell arrays, having a 240-nm diameter (inner, 200-nm polystyrene (PS) core; outer, 20-nm metal shell) and an 80-nm gap distance, exhibited a well-defined localized surface plasmon resonance (LSPR) peak at the near-infrared region. PS@Au nanoshell arrays showed a 55-nm red shift of the maximum LSPR wavelength of 885 nm after being exposed to a solution of bovine serum albumin (BSA) proteins for 18 h. On the other hand, in the case of Cu nanoshell arrays before/after incubation to the BSA solution, we found a 30-nm peak shifting. We could evaluate the difference in LSPR sensing performance by changing the metal materials. 相似文献
3.
采用Meisel方法利用氧化还原反应制各Ag纳米粒子溶胶,通过对Ag纳米粒子局域表面等离子体共振(SPR)吸收光谱的实时观测,研究了Ag纳米粒子形成的动力学过程,并着重探讨了温度对Ag纳米粒子生长过程的影响.实验发现,SPR吸收峰的移动与实验条件有密切关系.采用恒温磁力搅拌直接加热的方式,Ag纳米粒子的SPR吸收峰在整个反应过程中主要体现为红移,其反应动力学主要表现为零级反应,反应速率与温度符合Arrhenius关系,并粗略估算了一定反应条件下的反应活化能;而采用恒温磁力搅拌水浴加热方式,SPR吸收峰则呈现红移→蓝移→红移交替移动现象.初步探讨了SPR吸收峰的红、蓝移影响因素及其移动机理,得出SPR峰的移动方向是粒径大小与电荷转移相互竞争的结果. 相似文献
4.
表面等离子体激元共振技术是一种通过观察芯片金属薄膜表面厚度变化产生信号的光学技术,具有实时、灵敏度高、免标记等优点,本文通过辣根过氧化物酶的酶催化作用对过氧化氢进行实时快速检测。信号强度与过氧化氢浓度呈线性关系,辣根过氧化物酶共价结合至金膜表面保持了酶的活性。和其它方法相比较,该方法为过氧化氢的检测提供了一种选择。 相似文献
5.
Catherine Forest-Nault Jimmy Gaudreault Olivier Henry Yves Durocher Gregory De Crescenzo 《International journal of molecular sciences》2021,22(12)
Surface plasmon resonance (SPR)-based optical biosensors offer real-time and label-free analysis of protein interactions, which has extensively contributed to the discovery and development of therapeutic monoclonal antibodies (mAbs). As the biopharmaceutical market for these biologics and their biosimilars is rapidly growing, the role of SPR biosensors in drug discovery and quality assessment is becoming increasingly prominent. One of the critical quality attributes of mAbs is the N-glycosylation of their Fc region. Other than providing stability to the antibody, the Fc N-glycosylation influences immunoglobulin G (IgG) interactions with the Fcγ receptors (FcγRs), modulating the immune response. Over the past two decades, several studies have relied on SPR-based assays to characterize the influence of N-glycosylation upon the IgG-FcγR interactions. While these studies have unveiled key information, many conclusions are still debated in the literature. These discrepancies can be, in part, attributed to the design of the reported SPR-based assays as well as the methodology applied to SPR data analysis. In fact, the SPR biosensor best practices have evolved over the years, and several biases have been pointed out in the development of experimental SPR protocols. In parallel, newly developed algorithms and data analysis methods now allow taking into consideration complex biomolecular kinetics. In this review, we detail the use of different SPR biosensing approaches for characterizing the IgG-FcγR interactions, highlighting their merit and inherent experimental complexity. Furthermore, we review the latest SPR-derived conclusions on the influence of the N-glycosylation upon the IgG-FcγR interactions and underline the differences and similarities across the literature. Finally, we explore new avenues taking advantage of novel computational analysis of SPR results as well as the latest strategies to control the glycoprofile of mAbs during production, which could lead to a better understanding and modelling of the IgG-FcγRs interactions. 相似文献
6.
Park SJ Lee SW Jeong S Lee JH Park HH Choi DG Jeong JH Choi JH 《Nanoscale research letters》2010,5(10):1590-1595
For the improved surface plasmon-coupled photoluminescence emission, a more accessible fabrication method of a controlled
nanosilver pattern array was developed by effectively filling the predefined hole array with nanosilver colloid in a UV-curable
resin via direct nanoimprinting. When applied to a glass substrate for light emittance with an oxide spacer layer on top of the nanosilver
pattern, hybrid emission enhancements were produced from both the localized surface plasmon resonance-coupled emission enhancement
and the guided light extraction from the photonic crystal array. When CdSe/ZnS nanocrystal quantum dots were deposited as
an active emitter, a total photoluminescence intensity improvement of 84% was observed. This was attributed to contributions
from both the silver nanoparticle filling and the nanoimprinted photonic crystal array. 相似文献
7.
Anton Popov Viktorija Lisyte Asta Kausaite-Minkstimiene Eiva Bernotiene Almira Ramanaviciene 《International journal of molecular sciences》2022,23(20)
The application of antibody-functionalized quantum dots (QDs) in different areas has been widely described in the literature. However, a standard routine method for obtaining information on the conjugation efficiency of QDs with antibodies in terms of the interaction of the functionalized QDs with a specific antigen is still lacking. Herein, surface plasmon resonance (SPR) spectroscopy is proposed for this purpose. Gold-coated SPR sensor disks were modified with a self-assembled monolayer of 11-mercaptoundecanoic acid, and carbodiimide cross-linker chemistry was used to covalently immobilize the CD44 biomarker on the premodified surface (Au/CD44). Meanwhile, QDs functionalized with amine-derivatized polyethylene glycol (PEG) (QDs-NH2) were chosen for conjugation with antibodies because of their low non-specific adsorption on the Au/CD44 surface. Prior to conjugation, the surface binding capacity (Bmax) and equilibrium dissociation constant (KD) of the specific antibodies against CD44 (anti-CD44) were found to be 263.32 ± 2.44 m° and 1.00 × 10−7 ± 2.29 × 10−9 M, respectively. QDs-NH2 and anti-CD44 were conjugated at their initial molar ratios of 1:3, 1:5, 1:10 and 1:12. SPR measurements showed that the conjugates (QDs-anti-CD44) prepared using 1:10 and 1:12 molar ratios interacted comparably with immobilized CD44 biomarkers. The equilibrium angles in the case of 10- and 12-fold concentrations of anti-CD44 were calculated to be 60.43 ± 4.51 and 61.36 ± 4.40 m°, respectively. This could be explained by the QDs-NH2 and anti-CD44 having a similar surface loading (about four molecules per QDs-NH2) and similar hydrodynamic diameters, which were 46.63 ± 3.86 and 42.42 ± 0.80 nm for the 1:10 and 1:12 ratios, respectively. An initial QDs-NH2: anti-CD44 molar ratio of 1:10 was chosen as being optimal. SPR spectroscopy proved to be the right choice for QDs-anti-CD44 conjugation optimization, and can be used for the evaluation of conjugation efficiency for other nanostructures with various bio-recognition molecules. 相似文献
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Epitope‐Resolved Detection of Peanut‐Specific IgE Antibodies by Surface Plasmon Resonance Imaging 下载免费PDF全文
Min Shen Dr. Amit A. Joshi Dr. Raghu Vannam Dr. Chandra K. Dixit Prof. Dr. Robert G. Hamilton Prof. Dr. Challa V. Kumar Prof. Dr. James F. Rusling Prof. Dr. Mark W. Peczuh 《Chembiochem : a European journal of chemical biology》2018,19(3):199-202
Peanut allergy can be life‐threatening and is mediated by allergen‐specific immunoglobulin E (IgE) antibodies. Investigation of IgE antibody binding to allergenic epitopes can identify specific interactions underlying the allergic response. Here, we report a surface plasmon resonance imaging (SPRi) immunoassay for differentiating IgE antibodies by epitope‐resolved detection. IgE antibodies were first captured by magnetic beads bearing IgE ?‐chain‐specific antibodies and then introduced into an SPRi array immobilized with epitopes from the major peanut allergen glycoprotein Arachis hypogaea h2 (Ara h2). Differential epitope responses were achieved by establishing a binding environment that minimized cross‐reactivity while maximizing analytical sensitivity. IgE antibody binding to each Ara h2 epitope was distinguished and quantified from patient serum samples (10 μL each) in a 45 min assay. Excellent correlation of Ara h2‐specific IgE values was found between ImmunoCAP assays and the new SPRi method. 相似文献
9.
Site‐Specific Immobilization of the Peptidoglycan Synthase PBP1B on a Surface Plasmon Resonance Chip Surface 下载免费PDF全文
Dr. Inge L. van't Veer Nadia O. L. Leloup Dr. Alexander J. F. Egan Dr. Bert J. C. Janssen Dr. Nathaniel I. Martin Prof. Dr. Waldemar Vollmer Dr. Eefjan Breukink 《Chembiochem : a European journal of chemical biology》2016,17(23):2250-2256
Surface plasmon resonance (SPR) is one of the most powerful label‐free methods to determine the kinetic parameters of molecular interactions in real time and in a highly sensitive way. Penicillin‐binding proteins (PBPs) are peptidoglycan synthesis enzymes present in most bacteria. Established protocols to analyze interactions of PBPs by SPR involve immobilization to an ampicillin‐coated chip surface (a β‐lactam antibiotic mimicking its substrate), thereby forming a covalent complex with the PBPs transpeptidase (TP) active site. However, PBP interactions measured with a substrate‐bound TP domain potentially affect interactions near the TPase active site. Furthermore, in vivo PBPs are anchored in the inner membrane by an N‐terminal transmembrane helix, and hence immobilization at the C‐terminal TPase domain gives an orientation contrary to the in vivo situation. We designed a new procedure: immobilization of PBP by copper‐free click chemistry at an azide incorporated in the N terminus. In a proof‐of‐principle study, we immobilized Escherichia coli PBP1B on an SPR chip surface and used this for the analysis of the well‐characterized interaction of PBP1B with LpoB. The site‐specific incorporation of the azide affords control over protein orientation, thereby resulting in a homogeneous immobilization on the chip surface. This method can be used to study topology‐dependent interactions of any (membrane) protein. 相似文献
10.
Fujun Yao Ruiping Zhang He Tian Xiangjun Li 《International journal of molecular sciences》2012,13(9):11832-11843
The aggregation of β-amyloid peptide (Aβ) into fibrils plays an important role in the pathogenesis of Alzheimer’s disease (AD). Metal ions including copper and zinc are closely connected to the precipitation and toxicity of Aβ. In this study, a surface plasmon resonance (SPR) biosensor was constructed to investigate the interactions between Aβ and metal ions. Aβ peptide was immobilized on the SPR chip surface through a preformed alkanethiol self-assembled monolayer (SAM). Our observations indicate that the immobilized Aβ undergoes a conformational change upon exposure to the metal ions. A difference in metal binding affinity between Aβ1–28 and Aβ1–42 was also detected. The results suggest that SPR is an effective method to characterize the interactions between Aβ and metal ions. 相似文献
11.
《Ceramics International》2021,47(20):28557-28565
To reduce the energy consumption of cooling in the hot summer days, searching for novel NIR shielding materials for buildings is of great value. In this report, monodispersed F doped TiO2 nanocrystals with an average size of 8.6 nm were synthesized as novel solar shielding materials for energy-saving windows. All the products adopted an anatase TiO2 structure. After doping of F ions, the morphology of TiO2 was transformed from an irregular shape to a pseudospherical shape. The Raman shift and XPS depth analysis confirmed the successful doping of F− ions into the lattice oxygen sites in the TiO2 structure. The introduction of F− ions generated free electrons and bulk Ti3+ in TiO2 crystals, which activated a localized surface plasmon resonance (LSPR) absorption in the NIR region. Correspondingly, the NIR shielding performance of the TiO2 films improved with increasing F doping amounts. The NIR shielding value of the films increased from 1.3% to 43.2% when the molar ratio of F to Ti increased from 0 to 0.3. The reason can be attributed to the enhanced NIR absorption induced by the increased electron concentration after doping of fluorine ions. The F–TiO2 films showed superior visible transmittance (90.1–96.7%). Moreover, the F–TiO2 films lowered the indoor temperature of the heat box by 5.3 °C in the thermal tests. Overall, the prepared F–TiO2 nanocrystals show a great potential to be used for energy-saving windows. 相似文献
12.
Jian Zhu 《Nanoscale research letters》2009,4(9):977-981
Transverse surface plasmon resonances (SPR) in Au–Ag and Ag–Au core–shell structure nanowires have been investigated by means
of quasi-static theory. There are two kinds of SPR bands resulting from the outer surface of wall metal and the interface
between core and wall metals, respectively. The SPR corresponding to the interface, which is similar to that of alloy particle,
decreases and shifts obviously with increasing the wall thickness. However, the SPR corresponding to the outer surface, which
is similar to that of pure metal particle, increases and shifts slightly with increasing the wall thickness. A mechanism based
on oscillatory surface electrons under coulombic attraction is developed to illuminate the shift fashion of SPR from bimetallic
core–shell interface. The net charges and extra coulombic force in metallic wall affect the SPR energy and the shift fashion. 相似文献
13.
In this work, gallium doped copper sulfide (Ga-doped CuS) nanocrystals were prepared using a solvothermal method. The effects of Ga doping on the crystal structures, chemical composition, morphology, optical properties and thermal performance of copper sulfide (CuS) were investigated. The Ga-doped CuS nanocrystals had a hexagonal structure comparable to that of pure CuS. The Cu+/Cu2+ ratio first decreased and then increased with increasing Ga3+ doping. Both CuS and Ga-doped CuS exhibited nanoplate and nanorod morphologies. The visible transmittance of the Ga-doped CuS films was in the range of 61–77.1%. Importantly, the near-infrared (NIR) shielding performance of the films can be tuned by adjusting the concentration of the Ga dopant. The NIR shielding value of the optimal Ga-doped CuS film was 72.4%, which was approximately 1.5 times as high as that of the pure CuS film. This can be ascribed to the enhanced plasmonic NIR absorption that resulted from an increase in the hole concentration after doping with Ga3+ ions. In the thermal performance test, the Ga-doped CuS film lowered the interior temperature of the heat box by 9.1 °C. Therefore, the integration of good visible transmittance and high NIR shielding performance make the Ga-doped CuS nanocrystals a promising candidate for energy-efficient window coatings. 相似文献
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15.
Vera G. Praig Gaëlle Piret Xavier Castel Sabine Szunerits 《Electrochimica acta》2008,53(27):7838-7844
Two-dimensional gold nanostructures (Au NSs) were fabricated on amine-terminated indium tin oxide (ITO) thin films using constant potential electrolysis. By controlling the deposition time and by choosing the appropriate ITO surface, Au NSs with different shapes were generated. When Au NSs were formed directly on aminosilane-modified ITO, the surface roughness of the interface was largely enhanced. Modification of such Au NSs with n-tetradecanethiol resulted in a highly hydrophobic interface with a water contact angle of 144°. Aminosilane-modified ITO films further modified with colloidal Au seeds before electrochemical Au NSs formation demonstrated interesting optical properties. Depending on the deposition time, surface colors ranging from pale pink to beatgold-like were observed. The optical properties and the chemical stability of the interfaces were characterized using UV-vis absorption spectroscopy. Well-defined localized surface plasmon resonance signals were recorded on Au-seeded interfaces with λmax = 675 ± 2 nm (deposition time 180 s). The prepared interfaces exhibited long-term stability in various solvents and responded linearly to changes in the corresponding refractive indices. 相似文献
16.
Inside Cover: Site‐Specific Immobilization of the Peptidoglycan Synthase PBP1B on a Surface Plasmon Resonance Chip Surface (ChemBioChem 23/2016) 下载免费PDF全文
Dr. Inge L. van't Veer Nadia O. L. Leloup Dr. Alexander J. F. Egan Dr. Bert J. C. Janssen Dr. Nathaniel I. Martin Prof. Dr. Waldemar Vollmer Dr. Eefjan Breukink 《Chembiochem : a European journal of chemical biology》2016,17(23):2207-2207
17.
Arginine plays an important role in cell division and the functioning of the immune system. We describe a novel method by which arginine can be identified using an artificial monolayer based on surface plasmon resonance (SPR). The affinity of arginine binding its recognition molecular was compared to that of lysine. In fabrication of an arginine sensing interface, a calix[4]crown ether monolayer was anchored onto a gold surface and then characterized by Fourier Transform infrared reflection absorption spectroscopy, atomic force microscopy, and cyclic voltammetry. The interaction between arginine and its host compound was investigated by SPR. The calix[4]crown ether was found to assemble as a monolayer on the gold surface. Recognition of calix[4]crown monolayer was assessed by the selective binding of arginine. Modification of the SPR chip with the calix[4]crown monolayer provides a reliable and simple experimental platform for investigation of arginine under aqueous conditions. 相似文献
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《分离科学与技术》2012,47(5):710-719
In this work, adsorption isotherms and adsorption kinetics of CO2 on zeolite 13X and activated carbon with high surface area (AC-h) were studied. The adsorption isotherms and kinetic curves of CO2 on the adsorbents were separately measured at 328 K, 318 K, 308 K, and 298 K and with a pressure range of 0–30 bar by means of the gravimetric adsorption method. The mass transfer constants and adsorption activation energy Ea of CO2 on the adsorbents were estimated separately. Results showed that at very low pressure the amounts adsorbed of CO2 on the zeolite 13X was higher than that on the AC-h, while at higher pressure, the amounts adsorbed of CO2 on the AC-h was higher than that on the zeolite 13X since the AC-h has a larger surface area and a larger total pore volume compared to the zeolite 13X. The adsorption kinetics of CO2 can be well described by the linear driving force (LDF) model. With the increase of temperature, the mass transfer constants of CO2 adsorption on both samples increased. The adsorption activation energy Ea for CO2 on the two adsorbents decreased with the increase of pressure. Furthermore, at low pressure the Ea for CO2 adsorption on the zeolite 13X was slightly lower than that on the AC-h, while at higher pressure the Ea for CO2 adsorption on the zeolite 13X was higher than that on the AC-h. 相似文献