白腐真菌木质素降解酶的研究及应用进展

综述了白腐真菌木质素降解酶的研究进展，包括酶的结构和催化机理、酶的活性测定方法和酶的活性调控因素。此外，还介绍了木质素降解酶的实际应用及应用前景。     

固定化酶在污水处理中的成功开发

福建省重点科技项目“固定化酶技术在污染水源修复中的应用研究”，已通过有关专家鉴定。该项目开发的固定化酶技术及固定化酶反应器处理工业废水，具有创新性，工程应用显示了良好的效果。[第一段]     

酶工程技术与进展

简述了酶工程研究领域的主要内容。包括酶的分离与纯化，固定化酶、人工合成酶、模拟酶、ribozyme、抗体酶、杂交酶、非水系酶等的开发与应用，基因工程和蛋白质工程技术、分子压印技术、酶定向固定化技术、酶化学技术以及极端环境微生物和不可培养微生物的新酶种等现代酶工程技术研究领域的新进展及酶工程的应用。     

纤维素酶的概述

纤维素酶是一种重要的复合酶，在很多领域具有巨大的市场潜力，是四大工业酶种之一。文窜详细介绍了纤维索酶的基本概念、主要成分、组成部分、作用机制以及在各领域中的应用，并对纤维素酶的应用前景进行了展望。     

酶技术研究进展

酶技术是一个重要的研究领域，它对工业过程中酶的应用有重要影响。酶技术主要改进生化工艺过程中酶的新的应用方式，它对生物工艺技术工程有重要作用。酶的动力学拆分和在有机溶剂中的应用是酶技术的重要部分，近来这些方面已取得很大进展，生物技术的发展为石化工业的可持续发展带来新的曙光。     

烷基糖苷酯应用于口腔用品

本文介绍了葡聚糖酶，变位酶，脱氨酶与烷基糖苷酯在漱口剂，假牙清洗剂中的应用以及制备方法。     

影响加酶洗衣粉碱性蛋白酶活力测定的因素

本文对原加酶洗衣粉酶活力测定方法进行了较全面的考察，重点考察了洗衣粉对酶反应及其水解产物分析过程的影响。提出应用回归直线方程校正的方法，排除洗衣粉对酶水解产物分析的干扰，适用于不同加酶洗衣粉酶活力的测定，准确度在±5％以内，精密度在±3％以内，并对原方法作了一些改进。     

酶的固定化及应用

介绍酶的固定化方法，并对酶的各种固定化法进行了比较评价；综述了固定化酶在工业生产和生物传感器中的应用。     

美加净SOD牙膏的酶活稳定性研究

超氧化物歧化酶（简称SOD）活性稳定性是牙膏应用研究的技术关键。本文概述了SOD牙膏中各种因素对酶活的影响，开发一种酶稳定化方法，实践证明，在酶稳定性研究中取得了新的进展。     

酶在餐具洗涤剂中的应用

阐述了酶的来源及在极端环境中酶技术开发的现状，介绍了适合餐具洗涤剂用的蛋白酶和淀粉的新产品，给出了酶在餐具洗涤剂中的应用及餐具洗涤剂的发展趋势前景预测。     

非水介质中的酶促反应在有机合成中的应用

介绍了非水介质中酶促反应在几种有机合成反应中的应用,包括酶促酯化反应、转酯反应、聚合反应等。着重介绍了非水介质中的酶促反应用于手性化合物的拆分。展望了非水介质中酶促反应在医药、食品、高分子化学等领域的应用前景。     

一种新的非离子型乳化液膜体系的稳定性研究

制备了一种新的非离子型乳化液膜体系OP-4/D2EHPA/液体石蜡/煤油/盐酸,通过测定乳液电导率、破损率、溶胀率、乳滴粒径大小等,研究了乳化剂浓度、油内比、D2EHPA皂化度、内相盐酸浓度以及温度等因素对乳液稳定性的影响,并与以液膜传统专用乳化剂Span80、ENJ-3029、兰-113A所制得的W/O型乳液进行了加热破乳对比实验。结果表明该乳液体系表现出两个异常的稳定特性:(1)随着OP-4浓度的增加,乳液稳定性呈现先增加后下降的现象,OP-4在油相中的浓度为3%～5%(wt)时,乳液的稳定性较好。(2)当温度低于45℃时乳液表现出相当好的稳定性,温度超过50℃后乳液稳定性急剧下降。加热破乳对比实验表明,该乳液在60℃加热破乳10min,破乳率达100%,而分别以专用乳化剂Span80、ENJ-3029、兰-113A制备的乳液在60℃下加热60min仍不能破乳。通过控制适当条件,可以制备常温稳定性好而加热条件下容易破乳的乳化液膜体系。     

超临界CO2流体在化学反应中的应用

论述了超临界（亚临界）CO2在酶催化反应、氧化反应、催化加氢反应、烷基化反应、羰基化反应和聚合反应等方面的应用。指出了超临界（亚临界）CO2在高分子材料制备中具有重要的应用开发前景。     

Nucleic‐Acid‐Templated Enzyme Cascades

Cellular metabolism involves complex sequences of organized enzymatic reactions, known as metabolic pathways, that convert substrates into readily usable materials. In nature, these enzymatic complexes are organized in a well‐defined manner so that the cascade reactions are more rapid and efficient than they would be if the enzymes were randomly distributed in the cytosol. Development of artificial enzyme cascades that resemble nature's organization of sequentially assembled enzymes is of current interest due to its potential applications, from diagnostics to the production of high‐value chemicals. Nucleic acids and their nanostructures have been used to organize enzyme cascades and have been shown to enhance the efficiencies and rates of sequential reactions. Here we summarize the recent progress in the development of artificial enzyme cascades and sequential reactions by arranging enzymes on various DNA/RNA templates and discuss the future directions of this research endeavour.     

反胶团技术应用研究进展

反胶团具有独特的优良性能,有着十分广阔的应用前景,近年来正越来越多地受到关注。该文介绍了反胶团理论的最新研究进展,综述了反胶团技术在酶催化反应、萃取分离、纳米粒子的制备等方面的应用研究进展, 提出了反胶团技术目前存在的问题,并对其应用前景作了展望。引用文献22篇。     

Separation of Hydrogen Sulfide from Wastewater by Emulsion Liquid Membranes

Hydrogen sulfide was separated from highly saline wastewater by emulsion liquid membranes (ELMs). Such membranes consist of polyalkenyl succinimide as emulsifying agent, diethanolamine as carrier, kerosene as membrane, and sodium hydroxide as stripping solution. The effect of four surfactants on the stability of ELMs was investigated and every operational parameter was tested. The highest achievable separation efficiency was 99.73 % for a 100 mg L^–1 solution. Obviously, the salinity of the external phase has a negligible effect on the separation of H₂S using ELMs.     

反胶团酶反应研究进展

介绍了酶在反胶团中的主要特性,对反胶团酶反应的应用,动力学和过程的最新进展进行了综述。     

General rate equations and their applications for cyclic reaction networks: multi-pathway systems

Many chemical reactions of industrial importance involve complex reaction pathways and networks; and the determinations of the reaction rate laws are very difficult. The accurate or proper rate expression is the most desired information in design phase however. In this study, the network reduction technique and the Bodenstein approximation of quasi-stationary behavior of reaction intermediates were systematically applied to derive general rate and instantaneous rate ratio equations for multi-pathway reaction networks in homogeneous catalysis or enzyme system. Multiple small reaction cycles in a main cycle, and multiple-pathways stemming from an intermediate and ending at different nodes in a cycle were considered. The general rate and rate ratio equations derived in this study are applicable for most homogeneous catalytic reactions and enzymatic reactions. Two examples of multi-pathway cyclic enzyme reaction were used to illustrate the applications of the general rate and rate ratio equations for network elucidation.     

Recent progress in enzymatic functionalization of carbon-hydrogen bonds for the green synthesis of chemicals

Enzymatic reactions take place with high chemo-, regio-, and stereo-selectivity, appealing for the direct functionalization of abundant and inexpensive compounds with C-H bonds to make fine chemicals such as high-value intermediates and pharmaceuticals. This review summarizes recent progress in the enzymatic functionalization of C-H bonds with an emphasis on heme enzymes such as cytochrome P450s, chloroperoxidase and unspecific peroxygenases. Specific examples are discussed to elucidate the applications of the molecular and process engineering approaches to overcome the challenges hindering enzymatic C-H functionalization. Also discussed is the recent development of the chemo-enzymatic cascade as an effective way to integrate the power of metal catalysis and enzymatic catalysis for C-H functionalization.     

Recent progress in enzymatic functionalization of carbon-hydrogen bonds for the green synthesis of chemicals

Enzymatic reactions take place with high chemo-, regio-, and stereo-selectivity, appealing for the direct functionalization of abundant and inexpensive compounds with C-H bonds to make fine chemicals such as high-value intermediates and pharmaceuticals. This review summarizes recent progress in the enzymatic functionalization of C-H bonds with an emphasis on heme enzymes such as cytochrome P450s, chloroperoxidase and unspecific peroxygenases. Specific examples are discussed to elucidate the applications of the molecular and process engineering approaches to overcome the challenges hindering enzymatic C-H functionalization. Also discussed is the recent development of the chemo-enzymatic cascade as an effective way to integrate the power of metal catalysis and enzymatic catalysis for C-H functionalization.