Impacts of membrane flux enhancers on activated sludge respiration and nutrient removal in MBRs

This paper presents the findings of experimental investigations regarding the influence of 13 different flux enhancing chemicals (FeCl₃, polyaluminium chloride, 2 chitosans, 5 synthetic polymers, 2 starches and 2 activated carbons) on respirometric characteristics and nitrification/denitrification performance of membrane bioreactor (MBR) mixed liquor. Flux enhancing chemicals are a promising method to reduce the detrimental effects of fouling phenomena via the modification of mixed liquor characteristics. However, potentially inhibiting effects of these chemicals on mixed liquor biological activity triggered the biokinetic studies (in jar tests) conducted in this work. The tested polyaluminium chloride (PACl) strongly impacted on nitrification (−16%) and denitrification rate (−43%). The biodegradable nature of chitosan was striking in endogenous and exogenous tests. Considering the relatively high costs of this chemical, an application for wastewater treatment does thus not seem to be advisable. Also, addition of one of the tested activated carbons strongly impacted on the oxygen uptake rate (−28%), nitrification (−90%) and denitrification rate (−43%), due to a decrease of pH. Results show that the changes in k_La values were mostly not significant, however, a decrease of 13% in oxygen transfer was found for sludge treated with PACl.     

The impact of temperature and gas-phase oxygen on kinetics of in situ ammonia removal in bioreactor landfill leachate

Microcosm experiments aimed at defining a rate equation that describes how different environmental conditions (i.e., gas-phase oxygen concentrations, temperature and ammonia concentration) may impact in situ ammonia removal were conducted. Results indicate that ammonia removal can readily occur at various gas-phase oxygen levels (between 0.7% and 100%) and over a range of temperatures (22, 35 and 45 degrees C). Slowest rates occurred with lower gas-phase oxygen concentrations. All rate data, except at 45 degrees C and 5% oxygen, fit well (r2=0.75) to a multiplicative Monod equation with terms describing the impact of oxygen, pH, temperature and ammonia concentration. All ammonia half-saturation values are relatively high when compared to those generally found in wastewater treatment, suggesting that the rate may be affected by the mass transfer of oxygen and/or ammonia. Additionally, as the temperature increases, the ammonia half-saturation value also increases. The multiplicative Monod model developed can be used to aid in designing and operating field-scale studies.     

Comparison of recirculation configurations for biological nutrient removal in a membrane bioreactor

A 12-L lab-scale membrane bioreactor (MBR), consisting of an anaerobic and anoxic compartment followed by an oxic plate-frame membrane compartment, was evaluated for carbonaceous and nutrient removals by varying the recirculation of mixed liquor and permeate. The hydraulic retention times (HRTs) for the anaerobic, anoxic, and oxic compartments were 2, 2, and 8h, respectively. The solids residence time (SRT) for the oxic compartment was 25 days. Five different recirculation configurations were tested by recirculating mixed liquor and/or permeate recirculation equal to the influent flow rate (identified as 100%) into different locations of the anaerobic and anoxic compartments. Of the five configurations, the configuration with 100% mixed liquor recirculation to the anaerobic compartment and 100% permeate recirculation to the anoxic compartment gave the highest percentage removal with an average 92.3+/-0.5% soluble chemical oxygen demand (sCOD), 75.6+/-0.4% total nitrogen (TN), and 62.4+/-1.3% total phosphorus (TP) removal. When the mixed liquor and permeate recirculation rates were varied for the same configuration, the highest TP removal was obtained for 300% mixed liquor recirculation and 100% permeate recirculation (300%/100%) with a TP removal of 88.1+/-1.3% while the highest TN removal (90.3+/-0.3%) was obtained for 200%/300% recirculation. TN and TP concentrations as low as 4.2+/-0.1 and 1.4+/-0.2mg/L respectively were obtained. Mass loading rates were generally low in the range of 0.11-0.22kgCOD/kgMLSS/d due to high biomass concentrations within the oxic reactor (approx. 8000mg/L). The BioWin model was calibrated against one set of the experimental data and was found to predict the experimental data of effluent TN, TP, and NO(3)(-)-N but over-predicted sCOD and NH(3)-N for various recirculation rates. The anoxic heterotrophic yield for the calibrated model was 0.2kg biomass COD/kg COD utilized while the maximum growth rates were found to be 0.45day(-1) for mu(max-autotroph), 3.2day(-1) for mu(max-heterotroph), and 1.5day(-1) for mu(max-PAO).     

Membrane bioreactor operation at short solids retention times: performance and biomass characteristics

This study investigated the performance and biomass characteristics of a membrane bioreactor (MBR) and a completely mixed activated sludge (CMAS) system operated at short solids retention times (SRT) ranging from 0.25 to 5 d and hydraulic retention times of 3 and 6 h. The lab-scale reactors were fed with synthetic wastewater to ensure consistency in feed composition. Results show the MBR was capable of achieving excellent quality effluent regardless of the extremely short SRT. The MBR removal efficiencies ranged from approximately 97.3-98.4% (TCOD) in the MBR, compared to 77.5-93.8% (TCOD) and 94.1-97.0% (SCOD) in the CMAS. Nitrification completely ceased when SRT was < 2.5 d. The MBR biomass was composed of small, weak and uniform-sized flocs with large mass of short filamentous organisms and mainly dispersed microorganisms at SRT of 5 and 0.25 d, respectively. In contrast, the CMAS sludge was composed of large flocs with filamentous organisms as a backbone at SRT > 2.5 d. The CMAS flocs were smaller and weaker at shorter SRT. The MBR sludge contained a much higher fraction of non-flocculating microorganisms. This fraction increased significantly with decreasing SRT. It was found that the concentrations of protein and carbohydrates in the exocellular polymeric substances for both the MBR and the CMAS decreased with increasing F/M ratio or decreasing SRT. The combination of increasing amounts of non-flocculating microorganisms and a reduction of EPS at shorter SRT in both reactors contributed to deteriorating sludge settling properties. A significant presence of dispersed biomass and small flocs in MBR contributed to better reactor performance probably due to less mass transfer resistance.     

Comparative economic analysis of the impacts of mean cell retention time and denitrification on aeration systems

Biological nutrient removal is practiced in various modifications of the activated sludge process (ASP) throughout the world. This paper compares conventional, nitrifying-only and combined nitrifying/denitrifying (NDN) processes. The authors performed 113 oxygen transfer efficiency measurements with the off-gas method over 20 years. This dataset was analysed and used to perform an economic analysis for three example scenarios, one for each layout (conventional, nitrifying-only and NDN). Field oxygen transfer efficiency and relevant plant operative costs and credits were considered (i.e., aeration cost, sludge disposal cost, methane production credit). The conclusion is that NDN operations always have lower aeration costs, and generally have the lowest combined operating cost. Reduced aeration costs result because of improved aeration efficiency at higher mean cell retention times and the use of nitrate as an electron acceptor. The improved aeration efficiency overcomes the increased oxygen required at higher cell retention time due to cell decay.     

Modelling biological nutrient removal activated sludge systems - a review

The external nitrification (EN) biological nutrient removal (BNR) activated sludge (ENBNRAS) system shows considerable promise for full-scale implementation. As an aid for this implementation, a mathematical simulation model would be an invaluable tool. To develop such a model, a study was conducted to select the most suitable simulation model to serve as a starting point for further development. For this, the existing available simulation models for BNRAS systems are compared with one another and evaluated against experimental observations in the literature and on ENBNRAS systems. One process immediately apparent to be crucially important is the anoxic growth of phosphorus accumulating organisms (PAOs), with associated PAO denitrification and anoxic P uptake for polyP formation. These linked processes are lacking in the earlier kinetic simulation models for BNRAS systems, which were based on aerobic PAO growth and P uptake only, but have been incorporated into the more recent kinetic models. This provides a substantive body of information on modelling this aspect. Other processes of significance identified to require consideration are anaerobic slowly biodegradable COD (SBCOD) hydrolysis to readily biodegradable COD (RBCOD), and COD loss. Both processes have significant impact on the predicted BEPR performance. Due to the uncertainties associated with the mechanisms and quantification of these two processes, it is concluded that the most extensively validated kinetic simulation model should be selected for development, and that the omissions in this model should be addressed progressively, using the relevant information drawn from the existing models, the literature and observations on ENBNRAS systems.     

Baffled membrane bioreactor (BMBR) for efficient nutrient removal from municipal wastewater

Submerged membrane bioreactors (MBRs) are now widely used for various types of wastewater treatment. One drawback of submerged MBRs is the difficulty in removing nitrogen because intensive aeration is usually carried out in the tank and the MBRs must therefore be operated under aerobic conditions. In this study, the feasibility of treating municipal wastewater by a baffled membrane bioreactor (BMBR), particularly in terms of nitrogen removal, was examined. Simultaneous nitrification/denitrification in a single and small reaction tank was possible by inserting baffles into a normal submerged MBR as long as wastewater was fed in the appropriate way. To examine the applicability of the BMBR, pilot-scale experiments were carried out using real municipal wastewater. Although neither external carbon addition nor mixed liquor circulation was carried out in the operation of the BMBR, average removal rates of total organic carbon (TOC), total phosphorus (T-P) and total nitrogen (T-N) reached 85%, 97% and 77%, respectively, with the hydraulic retention time (HRT) of 4.7h. Permeability of the membrane could be maintained at a high level throughout the operation. It was found that denitrification was the limiting step in removal of nitrogen in the BMBR in this study. Various types of monitoring carried out in the BMBR also demonstrated the possibility of further improvements in its performance.     

COD and nitrogen removal by biofilms growing on gas permeable membranes

A bioreactor was constructed and used to treat a synthetic wastewater containing ammonium acetate and trace nutrients for about 190 days. The reactor was aerated by means of bundles of gas-permeable hollow-fiber membranes that were installed in the reactor. The membranes provided a specific surface area of 422 m(2)/m(3) and the external surface of the membranes rapidly became covered in an active biofilm. The membrane bundles were agitated by an internal gas recycle. The gas bubbles in the water encouraged fiber-fiber contact and were intended to control biofilm growth. Chemical oxygen demand (COD) removals in excess of 95% were achieved in a 6h nominal detention time. Nitrification developed rapidly and complete oxidation of the influent ammonium was evident within 20 days. Even though the reactor was equipped with a large membrane surface area, the oxygen was consumed within the biofilm growing on the membrane surface. As a result, the external dissolved oxygen (DO) dropped to zero and the reactor was able to support essentially complete denitrification. After about 3 months of operation the reactor showed excellent removals of both COD and inorganic nitrogen but the performance could not be sustained. Excess biofilm accumulation eventually contributed to a deterioration in process performance. This study demonstrates that while membrane aeration can provide simultaneous BOD and N removal in the same reactor, the membrane modules/bioreactor must be designed to allow for the development of thick biofilms. In addition, options for controlling the biofilm thickness need to be investigated.     

Peak flux performance and microbial removal by selected membrane bioreactor systems

A pilot study was conducted over a period of 18 months at the Point Loma Wastewater Treatment Plant (PLWWTP) in San Diego, CA to evaluate the operational and water quality performance of six selected membrane bioreactor (MBR) systems at average and peak flux operation. Each of these systems was operated at peak flux for 4 h a day for six consecutive days to assess peak flux performance. Virus seeding studies were also conducted during peak flux operation to assess the capability of these systems to reject MS-2 coliphage. When operating at steady state, these MBR systems achieved an effluent BOD concentration of <2 mg/L and a turbidity of <0.1 NTU. Peak flux for the MBR systems ranged from 56 to 76 L/m²/h (liters per square meter per hour) with peaking factors in the range of 1.5-3.2. When switching from average to peak flux operation, a reversible drop of 22-32% in temperature-corrected permeability was observed for all submerged MBR systems. The percent drop in permeability increased as MLSS concentration in the membrane tank increased from 11,100 mg/L to 15,300 mg/L and was observed to be highest for the system operating at highest MLSS concentration. Such trends were not observed with an external MBR system. Each MBR system was able to sustain a 4-h-a-day peak flow for six consecutive days with only moderate membrane fouling. The membrane fouling was quantified by measuring the drop in temperature-corrected permeability. This drop ranged from 13 to 33% over six days for different submerged MBR systems. The MBR systems achieved microbial removal in the range of 5.8-6.9 logs for total coliform bacteria, >5.5 to >6.0 logs for fecal coliform bacteria and 2.6 to >3.4 logs for indigenous MS-2 coliphages. When operating at peak flux, seeded MS-2 coliphage removal ranged from 1.0 to 4.4 logs, respectively. The higher log removal values (LRVs) for indigenous MS-2 coliphage among different MBR systems were probably the result of particle association of indigenous coliphage. Differences in membrane pore size (0.04-0.2 μm) amongst the MBR systems evaluated did not have a substantial impact on indigenous MS-2 coliphage removal, but seeded MS-2 coliphage removal varied among the different MBR systems.     

Long-term impact of anaerobic reaction time on the performance and granular characteristics of granular denitrifying biological phosphorus removal systems

Removal of nitrogen and phosphorus (P) from wastewater is successfully and widely practiced in systems employing both granular sludge technology and enhanced biological P removal (EBPR) processes; however, the key parameter, anaerobic reaction time (AnRT), has not been thoroughly investigated. Successful EBPR is highly dependent on an appropriate AnRT, which induces carbon and polyphosphate metabolism by phosphorus accumulating organisms (PAOs). Therefore, the long-term impact of AnRT on denitrifying P removal performance and granular characteristics was investigated in three identical granular sludge sequencing batch reactors with AnRTs of 90 (R1), 120 (R2) and 150 min (R3). The microbial community structures and anaerobic stoichiometric parameters related to various AnRTs were monitored over time. Free nitrite acid (FNA) accumulation (e.g., 0.0008–0.0016 mg HNO₂–N/L) occurred frequently owing to incomplete denitrification in the adaptation period, especially in R3, which influenced the anaerobic/anoxic intracellular intermediate metabolites and activities of intracellular enzymes negatively, resulting in lower levels of poly-P and reduced activity of polyphosphate kinase. As a result, the Accumulibacter-PAOs population decreased from 51 ± 2.5% to 43 ± 2.1% when AnRT was extended from 90 to 150 min, leading to decreased denitrifying P removal performance. Additionally, frequent exposure of microorganisms to the FNA accumulation and anaerobic endogenous conditions in excess AnRT cases (e.g., 150 min) stimulated increased extracellular polymeric substances (EPS) production by microorganisms, resulting in enhanced granular formation and larger granules (size of 0.6–1.2 mm), but decreasing anaerobic PHA synthesis and glycogen hydrolysis. Phosphorus removal capacity was mediated to some extent by EPS adsorption in granular sludge systems that possessed more EPS, longer AnRT and relatively higher GAOs.     

Meeting the phosphorus consent with biological nutrient removal under UK winter conditions

It is estimated that up to 342 wastewater treatment plants (Wwtps) in England and Wales will require a phosphorus (P) consent by 2010. Although biological P removal is considered to be the most sustainable option for P removal, it has always been problematic for plants that remove both nitrogen and P due to the inadequate concentration of organic material during wet periods. Two biological nutrient removal (BNR) configurations, the Johannesburg (JHB) process and a combined JHB and five-stage Bardenpho process, were evaluated over a period of 2 years to assess the impact of sewage strength on bio-P removal. The JHB achieved an average effluent total phosphorus (TP) of 2.4 mg/L and the combined JHB and five-stage process averaged 1.4 mg/L effluent TP. The major problems affecting the performance of both configurations were: dissolved oxygen (DO) in the recycled mixed liquor, nitrate in the return activated sludge (RAS) and low influent biological oxygen demand (BOD) concentrations. Acetate dosing proved successful as a source of volatile fatty acids (VFAs) in the anaerobic zone during periods of low-strength sewage. An acetate dosing strategy based on the influent flow rate to the plant was found to be a simple and effective technique that ensured that a consent of <1 mg TP/L could be met.     

Effects of integrated fixed film activated sludge media on activated sludge settling in biological nutrient removal systems

Integrated fixed film activated sludge (IFAS) is an increasingly popular modification of conventional activated sludge, consisting of the addition of solid media to bioreactors to create hybrid attached/suspended growth systems. While the benefits of this technology for improvement of nitrification and other functions are well-demonstrated, little is known about its effects on biomass settleability. These effects were evaluated in parallel, independent wastewater treatment trains, with and without IFAS media, both at the pilot (at two solids residence times) and full scales. While all samples demonstrated good settleability, the Control (non-IFAS) systems consistently demonstrated small but significant (p < 0.05) improvements in settleability relative to the IFAS trains. Differences in biomass densities were identified as likely contributing factors, with Control suspended phase density > IFAS suspended phase density > IFAS attached phase (biofilm) density. Polyphosphate content (as non-soluble phosphorus) was well-correlated with density. This suggested that the attached phases had relatively low densities because of their lack of anaerobic/aerobic cycling and consequent low content of polyphosphate-accumulating organisms, and that differences in enhanced biological phosphorus removal performance between the IFAS and non-IFAS systems were likely related to the observed differences in density and settleability for the suspended phases. Decreases in solids retention times from 8 to 4 days resulted in improved settleability and increased density in all suspended phases, which was related to increased phosphorus content in the biomass, while no significant changes in density and phosphorus content were observed in attached phases.     

Comparison of sludge characteristics and performance of a submerged membrane bioreactor and an activated sludge process at high solids retention time

This work aims to compare biomass structure and performance of a submerged membrane bioreactor (SMBR) and an activated sludge process (ASP) treating the same domestic wastewater. The influence of the separation technique (membrane filtration or settling) and operation at high sludge-retention time (SRT) were investigated. Over the entire range of SRT (10-110 days), the SMBR achieved very good organic removal efficiencies, ranging from 90.8+/-0.2% to 94.2+/-1.6% based on total COD (TCOD), whereas those of ASP were between 87.4+/-1.8% and 90.3+/-0.8%. The contribution of the membrane in the increase in performance was due to total suspended solid retention and also partly due to retention of proteins and polysaccharides of the sludge supernatant. No significant difference in excess sludge production was observed between the two processes operated at the same SRT, but sludge production in SMBR decreased from 0.31 to 0.13 g(VSS)g(COD)(-1) as SRT increased from 9 to 110 days. The difference in sludge characteristics and performance was especially pronounced as SRT increased, resulting in deterioration of sludge settleability and effluent quality of the ASP (filamentous bacteria, increase of protein and polysaccharide release). Membrane filtration induced accumulation of soluble and colloidal proteins and polysaccharides which were progressively degraded in the supernatant as the SRT increased. At similar SRT, no significant difference was observed in the amount of extractable exocellular polymeric substances (bound EPS) from ASP and SMBR sludge. However as the SRT increased, the total specific amount of bound EPS in flocs decreased and the ratio proteins/polysaccharides also decreased. Concomitantly, laser diffraction analysis, microscopic observations, turbidity and DSVI measurement showed that the SRT increase induced significant modifications in sludge morphology in SMBR: decrease in floc size, densification of aggregates, and development of non-flocculating organisms.     

Removal of ammonia from contaminated air in a biotrickling filter - denitrifying bioreactor combination system

The removal of gaseous ammonia in a system consisting of a biotrickling filter, a denitrification reactor and a polishing bioreactor for the trickling liquid was investigated. The system allowed sustained treatment of ammonia while preventing biological inhibition by accumulating nitrate and nitrite and avoiding generation of contaminated water. All bioreactors were packed with cattle bone composite ceramics, a porous support with a large interfacial area. Excellent removal of ammonia gas was obtained. The critical loading ranged from 60 to 120 gm(-3)h(-1) depending on the conditions, and loadings below 56 gm(-3)h(-1) resulted in essentially complete removal of ammonia. In addition, concentrations of ammonia, nitrite, nitrate and COD in the recycle liquid of the inlet and outlet of each reactor were measured to determine the fate of nitrogen in the reactor, close nitrogen balances and calculate nitrogen to COD ratios. Ammonia absorption and nitrification occurred in the biotrickling filter; nitrate and nitrite were biologically removed in the denitrification reactor and excess dissolved COD and ammonia were treated in the polishing bioreactor. Overall, ammonia gas was very successfully removed in the bioreactor system and steady state operation with respect to nitrogen species was achieved.     

Estimation of nitrogen removal rate in aqueous phase based on delta15N in microorganisms in solid phase

Microbial nitrification and denitrification are important processes for removing nitrogenous compounds in aqueous systems. Nitrogen removal rate estimation is essential for controlling nitrogen removal processes and modeling the nitrogen cycle in ecosystems. The model described the relationship between ammonium removal rate (aqueous phase) and the nitrogen stable isotope ratio (delta15N) of microorganisms (solid phase) when a coupled nitrification-denitrification process occurs and assimilation and advections are maintained in a steady state. An oxidation ditch in a municipal wastewater treatment plant was evaluated for 3 years using the model. The ammonium removal rate was calculated from the data of delta15N of the activated sludge, it correlated significantly with the observed removal rate. The isotope fractionation factor (epsilon) was determined to be -5.5 per thousand by using a nonlinear method. The model and obtained factor value were applicable for standard activated-sludge processes performed in parallel in the oxidation ditch and a river watershed. The model may help illustrate nitrogen behavior in ecosystems.     

Assessment of the nutrient removal performance in integrated constructed wetlands with the self-organizing map

The self-organizing map (SOM) model was applied to predict outflow nutrient concentrations for integrated constructed wetlands (ICWs) treating farmyard runoff. The SOM showed that the outflow ammonia-nitrogen concentrations were strongly correlated with water temperature and salt concentrations, indicating that ammonia-nitrogen removal is effective at low salt concentrations and comparatively high temperatures in ICWs. Soluble reactive phosphorus removal was predominantly affected by salt and dissolved oxygen concentrations. In addition, pH and temperature were weakly correlated with soluble reactive phosphorus removal, suggesting that soluble reactive phosphorus was easily removed within ICWs, if salt concentrations were low, and dissolved oxygen, temperature and pH values were high. The SOM model performed very well in predicting the nutrient concentrations with water quality variables such as temperature, conductivity and dissolved oxygen, which can be measured cost-effectively. The results indicate that the SOM model was an appropriate approach to monitor wastewater treatment processes in ICWs.     

Effect of soluble microbial products on microbial metabolisms related to nutrient removal

In this study, the effect of soluble microbial products (SMP) on the metabolisms related to phosphate or nitrogen removal of activated sludge was investigated. Two anaerobic-aerobic activated sludge processes were operated, one with a hydraulic retention time (HRT) of 48 h (RunL) and the other 6.4 h (RunS). The longer HRT of RunL was intended to promote the accumulation of SMPs in the supernatant. With the sludge from RunS and the supernatant from both of the runs, supernatant exchange batch experiments (SEBEs) were conducted, in which the acetate uptake rate and phosphate release rates under anaerobic conditions and the phosphate uptake rate under aerobic conditions were measured as these metabolisms are related to enhanced biological phosphorus removal. The nitrification rate was also measured. The statistical analyses of the results from the SEBEs showed that the supernatant from RunL had an inhibitory effect on the anaerobic acetate uptake and nitrification of the sludge from RunS. The cause of which was attributed to SMPs in the supernatant from RunL. As a result, the inhibitory effect of SMPs on nitrification and anaerobic acetate uptake was confirmed.     

The impact of alum based advanced nutrient removal processes on phosphorus bioavailability

Because eutrophication is a widespread consequence of wastewater discharges, there is a strong impetus to develop new approaches to remove phosphorus (P) from wastewater treatment plant (WWTP) effluents. We examined the effluents from a pilot plant that is testing various alum based processes for achieving > 99% P removal, however, it is not known how these advanced P removal technologies affect the bioavailability of P (BAP). We tested how the percent BAP (%BAP) varied with different P removal levels using an algal growth bioassay methodology. This facility reduced total P concentrations from ≈ 500 μg L⁻¹ in the pilot plant influent to 19 ± 4 (±SD) μg L⁻¹ in the final effluent, and our results showed that as the level of P removal increased, the %BAP of the product declined sharply, r² = 0.98. Prior to alum treatment, the influent had an average %BAP of 79 ± 13%, and after three steps of alum-based removal the %BAP averaged 7 ± 4%. Thus, this alum based P removal process was very effective at sequestering the P forms that most readily stimulate algal growth. Further, our results show the final BAP of the effluent was only ≈ 50% of the “reactive” P concentration. These results have important implications for nutrient management and trading schemes.     

Influence of detachment on substrate removal and microbial ecology in a heterotrophic/autotrophic biofilm

Competition between heterotrophic bacteria oxidizing organic substrate and autotrophic nitrifying bacteria in a biofilm was evaluated. The biofilm was grown in a tubular reactor under different shear and organic substrate loading conditions. The reactor was initially operated without organic substrate in the influent until stable ammonia oxidation rates of 2.1 g N/(m² d) were achieved. A rapid increase of fluid shear in the tubular reactor on day 156 resulted in biofilm sloughing, reducing the biofilm thickness from 330 to 190 μm. This sloughing event did not have a significant effect on ammonia oxidation rates. The addition of acetate to the influent of the reactor resulted in decreased ammonia oxidation rates (1.8 g N/(m² d)) for low influent acetate concentrations (17 mg COD/L) and the breakdown of nitrification at high influent acetate concentrations (55 mg COD/L). Rapidly increasing fluid shear triggered biofilm sloughing in some cases—but maintaining constant shear did not prevent sloughing events from occurring. With the addition of acetate to the influent of the reactor, the biofilm thickness increased up to 1350 μm and individual sloughing events removed up to 50% of the biofilm. Biofilm sloughing had no significant influence on organic substrate removal or ammonia oxidation. During 325 days of reactor operation, ammonia was oxidized only to nitrite; no nitrate production was observed. This lack of nitrite oxidation was confirmed by fluorescent in situ hybridization (FISH) analysis, which detected betaproteobacterial ammonia oxidizers but not nitrite oxidizers. Mathematical modeling correctly predicted breakdown of nitrification at high influent acetate concentrations. Model predictions deviated systematically from experimental results, however, for the case of low influent acetate concentrations.