Breakage and growth towards a stable aerobic granule size during the treatment of wastewater

To better understand granule growth and breakage processes in aerobic granular sludge systems, the particle size of aerobic granules was tracked over 50 days of wastewater treatment within four sequencing batch reactors fed with abattoir wastewater. These experiments tested a novel hypothesis stating that granules equilibrate to a certain stable granule size (the critical size) which is determined by the influence of process conditions on the relative rates of granule growth and granule breakage or attrition. For granules that are larger than the critical size, granule breakage and attrition outweighs granule growth, and causes an overall reduction in granule size. For granules at the critical size, the overall growth and size reduction processes are balanced, and granule size is stable. For granules that are smaller than the critical size, granule growth outweighs granule breakage and attrition, and causes an overall increase in granule size. The experimental reactors were seeded with mature granules that were either small, medium, or large sized, these having respective median granule sizes of 425 μm, 900 μm and 1125 μm. An additional reactor was seeded with a mixture of the sized granules to represent the original source of the granular sludge. The experimental results were analysed together with results of a previous granule formation study that used mixed seeding of granules and floccular sludge. The analysis supported the critical size hypothesis and showed that granules in the reactors did equilibrate towards a common critical size of around 600–800 μm. Accordingly, it is expected that aerobic granular reactors at steady-state operation are likely to have granule size distributions around a characteristic critical size. Additionally, the results support that maintaining a quantity of granules above a particular size is important for granule formation during start-up and for process stability of aerobic granule systems. Hence, biomass washout needs to be carefully managed to optimize granule formation during the reactor start-up.     

Airborne fungal cell fragments in homes in relation to total fungal biomass

Fungal exposure may induce respiratory symptoms. The causative agents are compounds in the fungal cell wall. Fragments of microbes may be present in air samples but are not measurable using conventional spore counting or by the determination of viable organisms. This study assesses the proportion of fungal cell biomass and endotoxin in different particle size fractions in air samples from homes. Air samples were collected from 15 homes using a cyclone sampler, collecting particles in three aerodynamic size fractions: <1.0, 1.0–1.8, and >1.8 μm. N‐Acetylhexosaminidase (NAHA) was determined as a marker of fungal cell biomass. Endotoxin was determined using the Limulus amebocyte lysate method. NAHA and endotoxin in the size range <1.0 μm comprised up to 63% (mean 22.7%) and 96.3% (mean 22.6%) of the total concentrations, respectively. There were significant relationships between the amounts of NAHA and endotoxin in the total amount and in the size fraction >1.8 μm but not in the smaller fractions. The results demonstrate significant amounts of fungal cell biomass and endotoxin in particles <1.0 μm. Homes with reported mold damage had a lower concentration of NAHA in particles <1.0 μm than homes without mold damage. To assess airborne exposure for diagnostic and preventive purposes, measurement techniques that include this fraction should be considered.     

The influence of hydraulic loads on depth filtration

The influence of hydraulic loads on the detachment of particles from the collector surface or from previously retained particles was observed in a packed glass beads column. A hydraulic shock load (i.e., 20% increase of flow rate) was applied after 4 h of particle attachment at a constant flow rate. A single type of particle suspension (Min-U-Sil 5, nearly pure SiO₂) and three different chemical conditions (pH control, alum and polymer destabilization) were utilized. The magnitude of particle detachment increased with increasing particle size for non-Brownian particles because more shear force was applied to large particles due to their large surface area. More favorable particles (i.e., particles with small surface charge) were detached to a lesser extent than unfavorable particles during the hydraulic shock loads application. This phenomenon can be caused by floc strength. In some cases, when the zeta potential of influent particles was relatively high, the magnitude of detachment of bigger particles (e.g., 4.0-5.0 μm) was less than that of smaller particles (e.g., 3.0-4.0 μm). This can be attributable to the breakup of detached flocs as an individual particle. It was also found that the shape of the curve relating the magnitude of particle detachment and particle size can be concave, linear, or convex depending on physicochemical conditions such as floc strength.     

Storage temperature affects distribution of carbon, VFA, ammonia, phosphorus, copper and zinc in raw pig slurry and its separated liquid fraction

Chemical-mechanical separation of pig slurry into a solid fraction rich in dry matter, P, Cu and Zn and a liquid fraction rich in inorganic N but poor in dry matter may allow farmers to manage surplus slurry by exporting the solid fraction to regions with no nutrient surplus. Pig slurry can be applied to arable land only in certain periods during the year, so it is commonly stored prior to field application. This study investigated the effect of storage duration and temperature on chemical characteristics and P, Cu and Zn distribution between particle size classes of raw slurry and its liquid separation fraction. Dry matter, VFA, total N and ammonium content of both slurry products decreased during storage and were affected by temperature, showing higher losses at higher storage temperatures. In both products, total P, Cu and Zn concentrations were not significantly affected by storage duration or temperature. Particle size distribution was affected by slurry separation, storage duration and temperature. In raw slurry, particles larger than 1 mm decreased, whereas particles 250 μm-1 mm increased. The liquid fraction produced was free of particles >500 μm, with the highest proportions of P, Cu and Zn in the smallest particle size class (<25 μm). The proportion of particles <25 μm increased when the liquid fraction was stored at 5 °C, but decreased at 25 °C. Regardless of temperature, distribution of P, Cu and Zn over particle size classes followed a similar pattern to dry matter.     

Application of fractal dimensions to study the structure of flocs formed in lime softening process

The use of fractal dimensions to study the internal structure and settling of flocs formed in lime softening process was investigated. Fractal dimensions of flocs were measured directly on floc images and indirectly from their settling velocity. An optical microscope with a motorized stage was used to measure the fractal dimensions of lime softening flocs directly on their images in 2 and 3D space. The directly determined fractal dimensions of the lime softening flocs were 1.11-1.25 for floc boundary, 1.82-1.99 for cross-sectional area and 2.6-2.99 for floc volume. The fractal dimension determined indirectly from the flocs settling rates was 1.87 that was different from the 3D fractal dimension determined directly on floc images. This discrepancy is due to the following incorrect assumptions used for fractal dimensions determined from floc settling rates: linear relationship between square settling velocity and floc size (Stokes’ Law), Euclidean relationship between floc size and volume, constant fractal dimensions and one primary particle size describing entire population of flocs. Floc settling model incorporating variable floc fractal dimensions as well as variable primary particle size was found to describe the settling velocity of large (>50 μm) lime softening flocs better than Stokes’ Law. Settling velocities of smaller flocs (<50 μm) could still be quite well predicted by Stokes’ Law. The variation of fractal dimensions with lime floc size in this study indicated that two mechanisms are involved in the formation of these flocs: cluster-cluster aggregation for small flocs (<50 μm) and diffusion-limited aggregation for large flocs (>50 μm). Therefore, the relationship between the floc fractal dimension and floc size appears to be determined by floc formation mechanisms.     

Removal of virus to protozoan sized particles in point-of-use ceramic water filters

The particle removal performance of point-of-use ceramic water filters (CWFs) was characterized in the size range of 0.02-100 μm using carboxylate-coated polystyrene fluorescent microspheres, natural particles and clay. Particles were spiked into dechlorinated tap water, and three successive water batches treated in each of six different CWFs. Particle removal generally increased with increasing size. The removal of virus-sized 0.02 and 0.1 μm spheres were highly variable between the six filters, ranging from 63 to 99.6%. For the 0.5 μm spheres removal was less variable and in the range of 95.1-99.6%, while for the 1, 2, 4.5, and 10 μm spheres removal was >99.6%. Recoating four of the CWFs with colloidal silver solution improved removal of the 0.02 μm spheres, but had no significant effects on the other particle sizes. Log removals of 1.8-3.2 were found for natural turbidity and spiked kaolin clay particles; however, particles as large as 95 μm were detected in filtered water.     

Influence of moisturizer and relative humidity on human emissions of fluorescent biological aerosol particles

Utilizing the ultraviolet light‐induced fluorescence (UV‐LIF) measurement technique as embodied in the Waveband Integrated Bioaerosol Sensor (WIBS‐4A), we evaluated the fluorescent particle emissions associated with human shedding while walking in a chamber. The mean emission rates of supermicron (1‐10 μm) fluorescent particles were in the range 6.8‐7.5 million particles per person‐h (~0.3 mg per person‐h) across three participants, for conditions when the relative humidity was 60%‐70% and no moisturizer was applied after showering. The fluorescent particles displayed a lognormal distribution with the geometric mean diameter in the range 2.5‐4 μm and exhibited asymmetry factors that increased with particle size. Use of moisturizer was associated with changes in number and mass emission rates, size distribution, and particle shape. Emission rates were lower when the relative humidity was reduced, but these differences were not statistically significant.     

Effect of particles and bioflocculation on ultraviolet disinfection of Escherichia coli

Presence of particles is known to decrease the effectiveness of ultraviolet (UV) disinfection by shielding the targeted microorganisms from UV light. This study aims to provide an in-depth understanding on the effect of particles and flocs on UV disinfection by using a stable, well-defined and well-controlled synthetic system that can simulate the bioflocculation of particles and microorganisms in water and wastewater samples. The synthetic system was created by using Escherichia coli, latex particles (1, 3.2, 11, 25, and 45 μm), alginate, and divalent cations; and the bioflocculation of particles was achieved naturally, as it would occur in the environment, without using chemical coagulants. E. coli was quantified before and after UV disinfection using membrane filtration. Even in the absence of particles, some of the self-aggregated E. coli could survive a UV dose of 90 mJ/cm². E. coli inactivation levels measured in the presence of particles were lower than the inactivation levels measured in the absence of particles. At low UV doses (<9 mJ/cm²), neither particle size nor degree of flocculation had a significant effect on the inactivation of E. coli. Particle size had a significant effect on the inactivation of E. coli only at high UV doses (80 mJ/cm²), and larger particles (e.g., 25 μm) protected bacteria more compared to smaller particles (e.g., 3.2 and 11 μm). What size of particles flocs were made of (3.2, 11, and 25 μm) did not make a significant difference on the inactivation levels of E. coli. For 3.2 μm particles, there was no significant difference in E. coli inactivation between non-flocculated and flocculated samples at any UV dose. For 11 and 25 μm particles, there was a significant difference in E. coli inactivation between non-flocculated and flocculated samples at 80 mJ/cm². Degree of flocculation became a significant factor in determining the number of surviving bacteria only at high UV doses and only for larger particles.     

Estimates of particulate matter inhalation doses during three-dimensional printing

Harmful emissions including particulates, volatile organic compounds, and aldehydes are generated during three-dimensional (3D) printing. Ultrafine particles are particularly important due to their ability to penetrate deep into the lung. We modeled inhalation exposure by particle size during 3D printing. A total of six thermoplastic filaments were used for printing under manufacturer's recommended conditions, and particle emissions in the size range between 10 nm and 10 μm were measured. The inhalation exposure dose including inhaled and deposited doses was estimated using a mathematical model. For all materials, the number of particles between 10 nm and 1 μm accounted for a large proportion among the released particles, with nano-sized particles being the dominant size. More than 1.3 × 10⁹ nano-sized particles/kgbw/g (95.3 ± 104.0 ng/kgbw/g) could be inhaled, and a considerable amount was deposited in respiratory regions. The total deposited dose in terms of particle number was 3.1 × 10⁸ particles/kgbw/g (63.6% of the total inhaled dose), and most (41.3%) were deposited in the alveolar region. The total mass of particles deposited was 19.8 ± 16.6 ng/kgbw/g, with 10.1% of the total mass deposited in the alveolar region. Given our findings, the inhalation exposure level is mainly determined by printing conditions, particularly the filament type and manufacturer-recommended extruder temperature.     

Modeling fine particle dispersion in an inhomogeneous electric field with a modified drift flux model

Dispersion of ultrafine particles (less than 0.1 μm) and accumulation mode particles (0.1–2.5 μm) remains as an area of major concern to microelectronic and semiconductor industry. A possible means of containing the dispersion of particulate pollutants is to subject them to electrostatic precipitation. The present study is concerned with the dispersion of particles in the presence of an inhomogeneous electric field. The widely accepted drift flux model is used to account for the drift flux induced by the inhomogeneous electric field. The mean turbulent flow field for the present analysis is obtained by solving the re-normalization group (RNG) k–? model with the aid of the open source CFD code – Open∇FOAM version 1.5. In addition to the flow field equations, the Poisson equation for the electric field, the charge continuity equation and the particle concentration equation are solved to obtain a complete solution for the present case. A comparison of the concentration field for a particle size of 0.1 μm with and without electric field reveals the impact of electric field on particle concentration distribution. The simulation results are compared with the available experimental data and numerical results.     

Effect of particle size on arsenic bioaccessibility in gold mine tailings of Nova Scotia

Tailings samples from the Goldenville and Montague abandoned gold mines in Nova Scotia, Canada were subjected to bioaccessibility tests to examine the effects of the choice of particle size fraction on the bioaccessibility of arsenic. The proportion of finer grains (< 150 μm) in this sample set varied from 6.0 to 66 wt.%. Samples were sieved to < 250, < 150, and < 45 μm particle size fractions. The arsenic bioaccessibility ranged from less than 1.0 to 48%, but no systematic variation was observed (p > 0.13) precluding the association of greater percent arsenic bioaccessibility with a specific particle size fraction, method or site. On the other hand, the highest bioaccessible arsenic concentrations (up to 5200 mg kg^− 1) were consistently observed in samples sieved to the < 45 μm particle size, for both the physiologically based extraction test and a glycine-buffered bioaccessibility method (in 89 and 87% of samples tested, respectively). This was due to higher total arsenic concentrations in the same particle size fraction. Grain maps obtained by X-ray absorption spectroscopy indicate that samples with the highest percent arsenic bioaccessibility contain amorphous pentavalent arsenic distributed throughout the sample as well as grains coated with pentavalent arsenic. Arsenic bioaccessibilities lower than 10% were found in samples with encapsulated arsenopyrite and some grains composed primarily of pentavalent arsenic. The < 45 μm particle size fraction appears to yield conservative (protective) estimates of the bioaccessible dose of arsenic, but wide variations exist in particle size distribution and arsenic bioaccessibility between samples. As well, sieving to < 45 μm may exclude potentially relevant particles by restricting the study to an average particle size that is smaller than the average size of particles found on human hands, and may unduly influence the resulting bioaccessibility measurements.     

Differences in microcystin production and genotype composition among Microcystis colonies of different sizes in Lake Taihu

The cyanobacterium Microcystis, which occurs as colonies of different sizes under natural conditions, can produce toxic microcystins (MCs). To monitor the toxicity and assess the risk of Microcystis blooms in Lake Taihu, it is important to investigate the relationship between MC production and Microcystis colony size. In this study, we classified Microcystis collected from Zhushan Bay of Lake Taihu during blooms into four classes with size of <50 μm, 50–100 μm, 100–270 μm and >270 μm and studied their differences in MC production and genetic structure. The results showed that colonies with size of <50, 50–100, 100–270 and >270 μm produced 12.2 ± 11.2%, 19.5 ± 7.9%, 61.3 ± 12.6%, and 7.0 ± 9.6% of total MC, respectively. The proportion of cell density of colonies with size of 50–100, 100–270 and >270 μm was positively correlated with MC concentration during blooms, while that of colonies with size of <50 μm was negatively correlated. The MC cell quota tended to be higher during blooms in colonies with larger size except that of colonies with size of 100–270 μm was higher than that of colonies with size of >270 μm from June 11 to September 16. Colonies with size of <50 μm showed the highest proportion of the less toxic MC congener MC-RR, and colonies with size of >100 μm showed higher proportion of the most toxic MC congener MC-LR than colonies with size of <100 μm. Real-time PCR indicated that larger colonies had higher proportion of potential toxic genotype. Principal component analysis of PCR-denaturing gradient gel electrophoresis profile showed that cpcBA and mcyJ genotype compositions were different between colonies with size of <50 μm and colonies with size of >50 μm, and cpcBA genotype composition was also different among colonies with size of 50–100 μm, 100–270 μm and >270 μm. These results indicated that MC cell quota and congener composition were different in Microcystis colonies with different sizes in Lake Taihu during blooms, and the differences in MC production in colonies with different size resulted chiefly from the difference in their genotype composition. Therefore, the authorities of water quality monitoring and drinking water supply service in Lake Taihu should be alert that the toxicity of Microcystis colony with different size was different during blooms, and the high abundance of colonies larger than 50 μm could be an indicator of relatively high bloom toxicity.     

Transport mechanisms of airborne particulate matters in partitioned indoor environment

The main objective of this study is to investigate the transport mechanisms of size-dependent airborne particulate matters in partitioned indoor environment. A three-dimensional Lagrangian particle tracking model is developed herein and validated by reliable experimental measurement. Four major particle-driving mechanisms (the gravitational force, the drag force, the Brownian motion force and the Saffman lift force) are considered in the model. Five kinds of particle transport mechanism scenarios are performed, including the dynamic equation scenario (all considered), the Brownian-motion-neglected scenario, the drag-force-neglected scenario, the lift-force-neglected scenario, and the inertial-force-neglected scenario (neglecting both the drag force and lift force). Seven different particle aerodynamic diameters (10, 5, 2.5, 1, 0.5, 0.1 and 0.05 μm), ranging from coarse to ultra-fine particle ranges, are used to investigate the relationship between particle size and each transport mechanism scenario. The results show that the influence of the drag force and the inertial force is significant for particle diameter larger than 1 μm, and the Brownian motion force is important for particle diameter smaller than 0.5 μm. The Saffman lift force cannot be neglected in a specific range of particle sizes between 2.5 and 5 μm.     

Particle characterization in retail environments: concentrations,sources, and removal mechanisms

Particles in retail environments can have consequences for the occupational exposures of retail workers and customers, as well as the energy costs associated with ventilation and filtration. Little is known about particle characteristics in retail environments. We measured indoor and outdoor mass concentrations of PM₁₀ and PM_2.5, number concentrations of submicron particles (0.02–1 μm), size‐resolved 0.3–10 μm particles, as well as ventilation rates in 14 retail stores during 24 site visits in Pennsylvania and Texas. Overall, the results were generally suggestive of relatively clean environments when compared to investigations of other building types and ambient/occupational regulatory limits. PM₁₀ and PM_2.5 concentrations (mean ± s.d.) were 20 ± 14 and 11 ± 10 μg/m³, respectively, with indoor‐to‐outdoor ratios of 1.0 ± 0.7 and 0.88 ± 1.0. Mean submicron particle concentrations were 7220 ± 7500 particles/cm³ with an indoor‐to‐outdoor ratio of 1.18 ± 1.30. The median contribution to PM₁₀ and PM_2.5 concentrations from indoor sources (vs. outdoors) was 83% and 53%, respectively. There were no significant correlations between measured ventilation rates and particle concentrations of any size. When examining options to lower PM_2.5 concentrations below regulatory limits, the required changes to ventilation and filtration efficiency were site specific and depended on the indoor and outdoor concentration, emission rate, and infiltration level.     

Size‐resolved fluorescent biological aerosol particle concentrations and occupant emissions in a university classroom

This study is among the first to apply laser‐induced fluorescence to characterize bioaerosols at high time and size resolution in an occupied, common‐use indoor environment. Using an ultraviolet aerodynamic particle sizer, we characterized total and fluorescent biological aerosol particle (FBAP) levels (1–15 μm diameter) in a classroom, sampling with 5‐min resolution continuously during eighteen occupied and eight unoccupied days distributed throughout a one‐year period. A material‐balance model was applied to quantify per‐person FBAP emission rates as a function of particle size. Day‐to‐day and seasonal changes in FBAP number concentration (N_F) values in the classroom were small compared to the variability within a day that was attributable to variable levels of occupancy, occupant activities, and the operational state of the ventilation system. Occupancy conditions characteristic of lecture classes were associated with mean N_F source strengths of 2 × 10⁶ particles/h/person, and 9 × 10⁴ particles per metabolic g CO₂. During transitions between lectures, occupant activity was more vigorous, and estimated mean, per‐person N_F emissions were 0.8 × 10⁶ particles per transition. The observed classroom peak in FBAP size at 3–4 μm is similar to the peak in fluorescent and biological aerosols reported from several studies outdoors.     

Effect of imposed anaerobic conditions on metals release from acid-mine drainage contaminated streambed sediments

Remediation of streams influenced by mine-drainage may require removal and burial of metal-containing bed sediments. Burial of aerobic sediments into an anaerobic environment may release metals, such as through reductive dissolution of metal oxyhydroxides. Mining-impacted aerobic streambed sediments collected from North Fork Clear Creek, Colorado were held under anaerobic conditions for four months. Eh, pH, and concentrations of Cd, Cu, Fe, Mn, and Zn (filtered at 1.5 μm, 0.45 μm, and 0.2 μm), sulfate, and dissolved organic carbon (DOC) were monitored in stream water/sediment slurries. Two sediment size fractions were examined (2 mm-63 μm and <63 μm). Sequential extractions evaluated the mineral phase with which metals were associated in the aerobic sediment. Released Cu was re-sequestered within 5 weeks, while Fe and Mn still were present at 16 weeks. Mn concentration was lower than in the initial stream water at and beyond 14 weeks for the smaller sized sediment. Cd was not released from either sediment size fraction. Zn was released at early times, but concentrations never exceeded those present in the initial stream water and all was re-sequestered over time. The greatest concentrations of Cu, Fe, Mn, and Zn were associated with the Fe/Mn reducible fraction. Sulfate and Fe were strongly correlated (r = 0.90), seeming to indicate anaerobic dissolution of iron oxy-hydroxy-sulfate minerals. DOC and sulfate were strongly correlated (r = 0.81), with iron having a moderately strong correlation with DOC (r = 0.71). Overall concentrations of DOC, sulfate, Cu, Fe, and Zn and pH were significantly higher (p < 0.05) in the water overlying the small sized sediment samples, while the concentrations of Mn released from the larger sized sediment samples were greater.     

Using portable particle sizing instrumentation to rapidly measure the penetration of fine and ultrafine particles in unoccupied residences

Much of human exposure to particulate matter of outdoor origin occurs inside buildings, particularly in residences. The particle penetration factor through leaks in a building's exterior enclosure assembly is a key parameter that governs the infiltration of outdoor particles. However, experimental data for size‐resolved particle penetration factors in real buildings, as well as penetration factors for fine particles less than 2.5 μm (PM_2.5) and ultrafine particles less than 100 nm (UFPs), remain limited, in part because of previous limitations in instrumentation and experimental methods. Here, we report on the development and application of a modified test method that utilizes portable particle sizing instrumentation to measure size‐resolved infiltration factors and envelope penetration factors for 0.01–2.5 μm particles, which are then used to estimate penetration factors for integral measures of UFPs and PM_2.5. Eleven replicate measurements were made in an unoccupied apartment unit in Chicago, IL to evaluate the accuracy and repeatability of the test procedure and solution methods. Mean estimates of size‐resolved penetration factors ranged from 0.41 ± 0.14 to 0.73 ± 0.05 across the range of measured particle sizes, while mean estimates of penetration factors for integral measures of UFPs and PM_2.5 were 0.67 ± 0.05 and 0.73 ± 0.05, respectively. Average relative uncertainties for all particle sizes/classes were less than 20%.     

Fabric changes induced by super-absorbent polymer on cement–lime stabilized excavated clayey soil

This paper studies the microstructure variation induced by super-absorbent polymer (SAP) to understand the mechanism of macroscopic strength improvement of stabilized soil. The fabric changes of cement–lime stabilized soil were analyzed with respect to the variation of SAP content, water content, lime content and curing time, using mercury intrusion porosimetry (MIP) tests. It can be observed that the delimitation pore diameter between inter- and intra-aggregate pores was 0.2 μm for the studied soil, determined through the intrusion/extrusion cycles. Experimental results showed that fabric in both inter- and intra-aggregate pores varied significantly with SAP content, lime content, water content and curing time. Two main changes in fabric due to SAP are identified as: (1) an increase in intra-aggregate pores (<0.2 μm) due to the closer soil–cement–lime cluster space at higher SAP content; and (2) a decrease in inter-aggregate pores represented by a reduction in small-pores (0.2–2 μm) due to the lower pore volume of soil mixture after water absorption by SAP, and a slight increase in large-pores (>2 μm) due to the shrinkage of SAP particle during the freeze–dry process of MIP test. Accordingly, the strength gain due to SAP for cement–lime stabilized soil was mainly due to a denser fabric with less inter-aggregate pores. The cementitious products gradually developed over time, leading to an increase in intra-aggregate pores with an increasing proportion of micro-pores (0.006–0.2 μm). Meanwhile, the inter-aggregate pores were filled by cementitious products, resulting in a decrease in total void ratio. Hence, the strength development over time is attributable to the enhancement of cementation bonding and the refinement of fabric due to the increasing cementitious compounds.     

Macroscopic and microscopic investigation of the structural characteristics of artificially structured marine clay

Artificial structural soil is the best medium for simulating the structural characteristics of undisturbed soil, which is a unique property of undisturbed soil. To figure out the macroscopic and microscopic structural properties of artificially structured soil, this study proposed a method of disposing artificial structural soil to simulate in situ marine clay. In addition, a series of one-dimensional compression tests considering three influencing factors—the initial void ratio, interparticle cementation strength (determined by cement content) and pore size distribution (determined by initial salt content)—were conducted to explore the macroscopic structural properties of artificially structured marine clay. Then, SEM and MIP tests were performed to investigate the microstructure properties of artificially structured marine clay. With respect to the macro-scale experiment, the results show as the initial void ratio increases, the yield stress decreases while the compressibility increases in postyield stage. The pore size distribution mainly affects deformation, which increases with the quantity of relatively large pores. In terms of the micro-scale test, we found that the general microstructure is composed of aggregates of small clay platelets, some of which are either attached to the aggregates or linked to the surrounding aggregates with cemented bridges crossing the interaggregate pores. The most and least common pore space types feature entrance pore diameters of 1 ～ 17 μm and larger than 17 μm, respectively. An increase in cement content causes increases in intra-aggregate pore space and interparticle cementation strength. Initial salt particle content exerts an influence predominantly in the pores with entrance diameters of 0.3 ～ 1 μm and 1 ～ 17 μm.     

Effects of ultrasound on suspended particles in municipal wastewater

The objective of this research is to explore the fundamental characteristics of how particles in wastewater respond to ultrasound, with an aim to improve wastewater disinfection. Particles of a predetermined size fraction and concentration were treated with varying doses of ultrasound at 20.3 kHz. Ultrasonic power transfer to the fluid was measured using calorimetry or acoustical measurements. Image analysis particle counting was used to measure the size distribution of particles before and after ultrasound treatment. The influence of three parameters: particle origin (raw wastewater or from the aeration basin of the activated sludge process), particle concentration, and particle size on the percentage of particle breakage after ultrasound treatment was compared. It was found that raw wastewater and aeration basin particles of the same size fraction (90-106 μm) responded to ultrasound in a similar way. Particle breakage was not affected by changes in particle concentration from 100 to 400 particles per mL. Larger wastewater particles (90-250 μm) were more susceptible to breakage than smaller ones (38-63 μm diameter). The percentage of particle breakage increased linearly with a logarithmic increase in the ultrasound energy density, that is the ultrasound energy delivered per unit volume of the sample (R² = 0.48-0.91). An expression that predicts the percent of particles broken as a function of ultrasound energy density is provided.