杀铃脲分子印迹聚合物制备及识别性能的研究

采用分子印迹技术,以杀铃脲为模板分子,甲基丙烯酸为功能单体,乙二醇二甲基丙烯酸酯为交联剂,在四氢呋喃溶剂中制备了杀铃脲分子印迹聚合物。以平衡吸附及高效液相色谱的方法对聚合物进行了评价。印迹聚合物对模板分子有较强的吸附能力和良好的选择性,可以很好的分离模板分子及其结构类似物。     

乙酰水杨酸分子印迹聚合物微球的制备及性能研究

以乙酰水杨酸为模板分子,采用沉淀聚合的方式制备了对乙酰水杨酸具有高选择性和亲和性的分子印迹聚合物微球。通过紫外光谱分析法考察了模板分子与功能单体作用规律,优选了功能单体与配合比例。等温吸附实验的结果显示,与相应的空白聚合物相比,印迹聚合物对模板分子具有更高的亲和性;Scatchard数学模型分析的结果表明,印迹聚合物中主要存在一类亲和性质相同的结合位点,其最大表观结合量为37.97 mg/g。选择性吸附实验表明,制备的印迹聚合物对模板分子具有良好的选择性识别能力。     

加替沙星分子印迹聚合物的制备及其性能研究

以加替沙星为模板分子,甲基丙烯酸为功能单体,乙二醇二甲基丙烯酸酯为交联剂,聚乙烯醇为分散剂,氯仿为溶剂,悬浮聚合法制备了分子印迹聚合物。通过静态吸附,动态吸附及选择性吸附对制备的聚合物进行性能测定,并且通过Scatchard分析以对聚合物的吸附行为进行分析。结果表明,印迹聚合物有两类结合位点,为多分子层吸附过程,对目标分子具有良好的选择性。     

罗丹明B分子印迹聚合物的制备及分子识别性能研究

以三苯甲烷类碱性染料罗丹明B为模板分子,丙烯酰胺为功能分子,N,N-亚甲基双丙烯酰胺(MBA)为交联剂,采用表面修饰分子印迹法,制备对罗丹明B具有特定亲和选择性的分子印迹聚合物。应用红外光谱对印迹聚合物进行表征,通过静态吸附实验对聚合物的吸附动力学进行分析,采用平衡结合实验评价了分子印迹聚合物的吸附性能。结果表明,采用表面修饰分子印迹法所制备的印迹聚合物对罗丹明B表现出较好的特异吸附,对模板分子最大吸附量值为96μmol/g。     

沉淀聚合法制备Boc-L-苯丙氨酸印迹聚合物

以Boc-L-苯丙氨酸为模板分子,应用沉淀聚合法制备了对Boc-L-苯丙氨酸具有特异性吸附的分子印迹聚合物。通过平衡吸附和高效液相色谱的方法对印迹聚合物进行评价,结果表明印迹聚合物具有高吸附效率和选择性,实现了消旋混和物的快速基线分离。     

球形印迹交联壳聚糖聚合物的制备及其对尿素的吸附性能研究

采用乳液聚合法制备了球形尿素-壳聚糖分子印迹聚合物。通过正交试验优化了印迹聚合物的制备条件:在乙酸体积分数为2%,戊二醛用量为1 mL,转速为900 r/min,模板分子用量为3 g的条件下制备的印迹聚合物对尿素的吸附量为7.16 mg/g,非印迹聚合物对尿素的吸附量为3.84 mg/g。聚合物的红外图谱研究表明,戊二醛参与了交联反应且模板分子尿素被印迹在聚合物上,聚合物经洗脱后模板分子被基本除去。分子印迹聚合物表面具有均一选择性的吸附位点,最大表观吸附量为9.69 mg/g。     

茶碱印迹聚合物的合成与性能研究

利用分子印迹技术合成了以茶碱为模板分子的印迹聚合物 ,并对聚合反应条件进行了讨论。采用紫外分光光度法对印迹聚合物的吸附性能和选择性识别能力进行了研究。     

结晶紫印迹材料的制备及其在鲤鱼样品分析中的应用

以硅胶为基质,结晶紫为模板分子,甲基丙烯酸(MAA)为功能单位,N,N-亚甲基丙烯酰胺(MBA)为交联剂,硅胶为载体,采用表面修饰分子印迹法,制备对结晶紫具有特定亲和选择性的分子印迹聚合物。应用红外光谱对印迹聚合物进行表征,通过静态吸附实验优化实验条件,对聚合物的吸附行为进行分析,采用平衡结合实验评价了分子印迹聚合物的吸附性能。结果表明,采用表面修饰分子印迹法所制备的印迹聚合物对结晶紫表现出较好的特异吸附,对模板分子最大吸附量值为22. 04 mg/g。     

水相中马尿酸分子印迹聚合物的制备及其性能研究

采用分子印迹技术,以马尿酸为模板分子,在水相中制备其印迹聚合物。用于液相色谱固定相,结果表明,该聚合物固定相表现出良好的分离选择性,能够使马尿酸与和其结构相似的苯甲酸及咖啡酸快速基线分离。并通过平衡吸附法分析了印迹聚合物的识别特性,结果表明分子印迹聚合物对马尿酸有特异性吸附。     

壳聚糖表面胰蛋白酶分子印迹聚合物的制备及性能的研究

在壳聚糖表面通过希夫碱反应嫁接一层戊二醛,形成核-壳结构微球。然后在这个核-壳微球上,以胰蛋白酶为模板分子,3-氨基苯硼酸为功能单体,制备了胰蛋白酶分子印迹聚合物,通过静态吸附法,研究了聚合物的吸附性能。结果表明,印迹聚合物对模板分子有较高吸附容量和特异选择性。为从蛋白质混和溶液中分离富集胰蛋白酶提供了新的材料和方法。     

利福平分子印迹聚合物的制备及其吸附行为

以利福平为模板分子、甲基丙烯酸为功能单体、乙二醇二甲基丙烯酸酯为交联剂,制备了利福平分子印迹聚合物. 采用平衡结合方法和Scatchard模型评价了该聚合物的结合特性,考察了其吸附行为. 结果表明,利福平分子印迹聚合物中形成了两类不同的结合位点,得到高亲和力结合位点的离解常数和最大表观结合量分别为31.5 mg/mL和23.34 mg/g,低亲和力结合位点的离解常数和最大表观结合量分别为9.22 mg/mL和12.86 mg/g. 实验结果显示,利福平分子印迹聚合物对利福平呈现出了高的选择吸附特性.     

Preparation and characterization of uniform molecularly imprinted polymer beads for separation of triazine herbicides

Uniform molecularly imprinted polymer beads were synthesized by precipitation polymerization for separation of triazine herbicides. A series of imprinted polymers were prepared using ametryn as template and divinylbenzene as crosslinking monomer, in combination with three different functional monomers under different solvent conditions. Under optimized reaction conditions, we obtained uniform molecularly imprinted polymer microspheres that display favorable molecular binding selectivity for triazine herbicides. The imprinted polymer beads synthesized using methacrylic acid as functional monomer in a mixture of methyl ethyl ketone and heptane showed the best results in terms of particle size distribution and molecular selectivity. Compared with nonimprinted polymer microspheres, the imprinted microspheres displayed significantly higher binding for a group of triazine herbicides including atrazine, simazine, propazine, ametryn, prometryn, and terbutryn. For the first time, precipitation polymerization has been used to produce highly uniform imprinted microspheres suitable for affinity separation of triazine herbicides. © 2012 Wiley Periodicals, Inc. J Appl Polym Sci, 2012     

Tocopherol‐targeted membrane adsorbents prepared by hybrid molecular imprinting

A hybrid molecular imprinting technique for a tocopherol target enabled the preparation of novel membrane adsorbents. α‐Tocopherol methacrylate was used as a functional monomer, and molecular imprinted polymer (MIP) was prepared by copolymerization with divinylbenzene. After the copolymer was granulated, several hybrid molecular imprinted membranes (HMIP) containing the polymer powders were prepared by using polymer scaffolds such as polysulfone (PSf), cellulose acetate (CA), and nylon (Ny). All HMIP membranes prepared by the phase inversion technique showed selective binding of α‐tocopherol (α‐Toc) over its derivative, δ‐tocopherol (δ‐Toc). The imprint efficiencies were 0.49 for the MIP powder and 0.60, 0.64, and 0.53 for the PSf, Ny, and CA‐HMIP systems, respectively. These HMIP membranes retained their binding capacity without losing significant selectivity relative to the MIP powder. Our results demonstrated that the α‐Toc‐imprinted sites were responsible for the selective binding of the target molecules by the HMIP membranes. The membranes' binding behavior was analyzed using Scatchard plots; a heterogeneous distribution of binding sites was observed in both the MIP polymer and HMIP membranes. POLYM. ENG. SCI., 2008. © 2008 Society of Plastics Engineers     

Specific binding of cholic acid by cross-linked polymers prepared by the hybrid imprinting method

A cholic acid-imprinted polymer has been prepared by a hybrid imprinting method involving the use of a template-containing monomer, 3α-methacryloyl cholic acid methyl ester. The removal of the template can afford the polymer with cavities and carboxylic acid groups well positioned in these cavities for the specific binding of cholic acid via hydrogen bonding. The maximum binding capabilities for the imprinted and non-imprinted polymers were determined to be 344.8 and 43.6 μmol/g, respectively. Scatchard analysis indicates the existence of two kinds of binding sites on the imprinted polymer. The imprinted polymers exhibit high affinity and good recognition selectivity for cholic acid in comparison to other compounds with similar molecular structures. The size-specific binding and hydrogen bonding may be both important for the binding of cholic acid by the imprinted polymers.     

3,5,7,3＇,4＇,5＇-六羟基黄酮分子印迹聚合物制备及性能表征

采用分子印迹技术合成了对3,5,7,3＇,4＇,5＇-六羟基黄酮具有高效选择性的分子印迹聚合物,通过静态吸附的方法,利用Scatchard模型,研究了分子印迹聚合物的特异性识别能力。实验表明,以甲基丙烯酸为功能单体,在甲苯溶剂中聚合得到的分子印迹聚合物对3,5,7,3＇,4＇,5＇-六羟基黄酮具有较大的吸附富集能力和识别特性。其饱和吸附容量达到30.8 mg/g。     

表面分子印迹材料制备研究进展

表面分子印迹技术是通过把分子识别位点建立在印迹材料的表面,来提高识别位点与印迹分子的结合速度,进一步加强印迹材料吸附分离效率。从无机硅胶材料和聚合物材料两方面综述了表面分子印迹材料制备技术发展现状,着重评述了表面分子印迹聚合物微球的制备方法及相应产品的性能,并指出表面分子印迹技术存在的不足。     

Dummy template molecularly imprinted polymer for omeprazole and the study of its drug binding and release properties

In this study, we tried to prepare an omeprazole (OMP)‐imprinted polymer and study its binding and release properties in an aqueous media. Because of the instability of OMP under polymerization conditions and the inability of the molecule to form effective interactions with monomers, pantoprazole (PANTO) was used as a dummy template for the imprinting process. Different monomers and solvents were evaluated in polymerization. The optimized imprinted polymer was prepared in chloroform as a porogen. Also, 4‐vinylpyridine and ethylene glycol dimethacrylate were selected as a functional monomer and a crosslinker, respectively. The optimized imprinted polymer was evaluated in a binding study. The binding and release properties of the polymer were then investigated at different pHs. Our data indicated a higher affinity of the imprinted polymer to PANTO and OMP than that of nonimprinted polymer (NIP). The maximum percentage of OMP released from the imprinted polymer was 36–41%, whereas that for the NIP was 74–85%. These data were related to the 38–43 and 29–34 μg of OMP released from the imprinted polymer and NIP, respectively. Also, the protective effect of the imprinted polymer for OMP at pH 2 was greater than that of the nonimprinted one. This study revealed that the dummy template molecular imprinting was an effective method for preparing selective imprinted cavities in a polymeric matrix, especially for the molecules that were unstable during polymerization or unable to establish effective bonds with the monomers. © 2013 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 130: 4165–4170, 2013     

单体对分子印迹聚合物分子识别能力的影响：量子化学计算与实验研究

采用密度泛函方法计算功能单体与印迹分子的结合能,以与目标分子结合能最大的单体分子来合成分子印迹聚合物.为此,以茶碱为印迹分子,氯仿为溶剂,首先计算了茶碱与甲基丙烯酸、丙烯酰胺和三氟甲基丙烯酸的结合能,其强度顺序为：三氟甲基丙烯酸 > 甲基丙烯酸 > 丙烯酰胺.然后以茶碱为印迹分子、氯仿为溶剂、二甲基丙烯酸乙二醇酯为交联剂,分别采用上述3种单体合成分子印迹聚合物并测定了其分子识别能力,实验结果和量子化学计算结果具有一致性.最后,采用1H NMR考察了茶碱和上述3种单体之间的氢键作用,揭示出二者相互作用的内在机制.研究结果表明量子计算方法可以应用于合成分子印迹聚合物时单体的选择.     

Molecularly imprinted polymers via living radical polymerization: Relating increased structural homogeneity to improved template binding parameters

This work examined imprinted polymer networks prepared via controlled/living radical polymerization (LRP) and conventional radical polymerization (CRP) on chain growth, network formation, and efficiency of producing molecularly imprinted, macromolecular memory sites for the template molecule, diclofenac sodium. LRP extended the reaction-controlled regime of the polymerization reaction and formed more homogeneous polymer chains and networks with smaller mesh sizes. In addition, LRP negated the effect of the template on polymer chain growth resulting in polymers with a more consistent PDI independent of template concentration in the pre-polymerization solution. Improved network homogeneity within imprinted poly(HEMA-co-DEAEM-co-PEG200DMA) networks prepared via LRP resulted in a 38% increase in template binding affinity and 43% increase in the template binding over imprinted networks prepared via CRP and a 97% increase in affinity and 130% increase in capacity over non-imprinted networks prepared by LRP. By varying certain parameters, it was possible to create imprinted networks with even higher template binding affinities (155% over non-imprinted) and capacities (261% over non-imprinted). This work is the first to conclusively demonstrate that the observed improvement in binding parameters in weakly crosslinked, imprinted polymer networks could be explained by the more uniform molecular weight evolution associated with the LRP mechanism and the longer lifetime of an active polymer chain relative to the total polymerization time, which allowed for the formation of a more homogenous imprinted polymer network.