具养颜功效的乳酸菌发酵型泡菜工艺条件研究

以包心芥菜为原料,添加党参、黄芪、枸杞、桂圆制作泡菜。采用正交试验得出最佳工艺条件为:药材量8%、盐浓度4%、糖浓度1%、接种量3%、发酵温度30℃、发酵时间4d。根据此条件可开发出具有养颜、抗衰老功效的新型泡菜保健食品。     

4种香辛料对泡菜发酵过程中乳酸菌生长的影响

研究了大蒜、茴香、生姜、辣椒 4种香辛料对蔬菜发酵过程中的主要参与菌种肠膜明串珠菌、植物乳杆菌生长的影响。香辛料经预处理后 ,以不同的添加量加入含大白菜的泡菜水中 ,经灭菌 ,分别接种肠膜明串珠菌、植物乳杆菌 ,3 0℃培养后 ,在波长 480nm处测定其OD值。发现大蒜、茴香、辣椒 3种香辛料对 2种乳酸菌的生长都具有明显促进作用 ,对明串珠菌的作用强于植物乳杆菌 ;2 %的辣椒和茴香效能最高。这一结果为进一步改进酸泡菜的生产工艺过程、延长产品的货架期、提高产品的质量提供了理论指导。     

乳酸菌发酵药膳保健泡菜的研制

将党参、黄芪、当归、红枣四味配伍中药材适量添加到大豆泡菜中,采用优良乳酸菌纯种发酵,研制营养全面、风味独特、经济适用且兼具医疗保健价值的新型药膳保健泡菜.以接种量、加盐量、加糖量、药材量为四个因素,通过正交试验得乳酸菌发酵药膳保健泡菜的最佳工艺条件为:接种量6％、加盐量4％、加糖量1％、药材量12％.在此条件下制作的乳酸菌发酵药膳保健泡菜感官评定为优,亚硝酸盐含量为7.95mg/kg,总酸含量(以乳酸计)为1.09％,氨基酸态氮含量为0.055 g/dL,乳酸菌含量为1.35×108 cfu/mL,未检测出大肠菌群和致病菌,所有指标均符合国家相关标准.     

什锦泡菜汗中乳酸菌含量的研究

本文研究会锦泡菜汗中的乳酸菌含量情况,结果表明,什锦泡菜比普通泡菜汁中的乳酸菌含量高,维持高含菌量的时间较好,因此,可根据各人口味选择不同菜料制成营养价值更高的什锦泡菜。     

罗汉果在乳酸菌发酵食品泡菜中的应用研究

以包心芥菜和胡萝卜为原料,两者比为3∶2,添加罗汉果水煎汁,采用正交实验方法制作泡菜,并结合泡菜中乳酸菌数及维持高含菌量的时间,得出最佳工艺条件为:罗汉果汁量4%、盐浓度4%、糖浓度1%、接种量3%、发酵温度30℃,发酵时间5d。根据此条件,开发出了具有消暑止渴、清热解毒、止咳化痰等功效的新型泡菜保健食品。      

提高泡菜汁中乳酸菌含量的研究

该研究应用的是一种新的高层半固体琼脂试管法，检测在不同条件下制成的泡菜汁中的乳酸菌活菌数。试验结果表明，盐浓度以4～6％为宜，物料以胡萝卜最好，调料以米酒最佳，含酒量为4％较好，30t时投料3天后，即可达到最高含菌量，在多数样品中，含菌量可达108～109个／ml，pH值稳定在3．1～3．3之间。此外，文中还讨论了利用泡菜汁开发廉价的天然微生态调节剂的可能性。     

泡菜中乳酸菌的研究进展

乳酸菌在泡菜发酵过程中有重要意义,为了克服传统方法生产泡菜的不足,需进一步研究制作泡菜的乳酸菌发酵剂。该文对泡菜中乳酸菌的主要种类及发酵类型、影响乳酸菌发酵的主要因素、乳酸菌发酵剂的来源、在泡菜中的应用及基因组学等进行了综述,并展望了其发展趋势。     

泡菜用乳酸菌发酵剂培养基的优化

通过试验确定适合于工业化生产的泡菜用乳酸菌培养条件，为纯菌发酵泡菜工艺的推广及生产直投式乳酸菌发酵剂提供参考。     

泡菜中乳酸菌的分离、鉴定和生产初试

从泡菜汁中分离乳酸菌,筛选出3株产酸量较高、生长良好的菌株。从该菌的形态特征、菌落特征、生理生化特征进行鉴定,判定结果A8、B2为弯曲乳杆菌,B4为植物乳杆菌。以其为纯培养发酵剂制备萝卜泡菜,质量优于自然发酵产品。     

乳酸菌发酵型枸杞泡菜最佳工艺条件研究

以包心芥菜和白萝卜为原料,在两者比为3:2下,添加枸杞制得泡菜.采用正交试验,得出最佳工艺条件为:枸杞量6%,盐浓度4%,糖浓度1%,接种量3%,发酵温度30℃,发酵时间4d.用此工艺制作的泡菜具有很好的色、香、味.文中最后还简要讨论了枸杞泡菜的发展前景.     

乳酸菌代谢工程的研究与应用

综述了乳酸菌代谢工程的研究与应用，阐述了利用基因工程技术改良乳酸菌发酵剂产双乙酰能力、蛋白质水解能力、胞外多糖的合成能力以及抗杂菌病原菌污染能力4个方面的最新研究进展。     

LACTIC ACID BACTERIA AND MALOLACTIC FERMENTATION IN THE MANUFACTURE OF SPANISH CIDER

Numeration, isolation and identification of the lactic acid bacteria found in Asturian cider during processing and storage has been carried out. The species Leuconostoc oenos was predominant during both alcoholic and malolactic fermentations. In most of the ciders studied the malolactic fermentation and the alcoholic fermentation started at the same time; production of D-lactate as well as a decrease of L-lactate were to be found once the malolactic fermentation was complete .     

野生蓝莓汁乳酸菌饮料的研究

通过采用四因素三水平的正交试验,对野生蓝莓汁乳酸菌饮料的最佳工艺配方进行研究.结果表明:蓝莓汁8%、白砂糖9%、发酵乳25%、乳化稳定剂0.30%,42℃发酵4 h.菌种比1:1,接种量2.5%,VC含量高达25 g/L.     

ASSESSMENT OF POTENTIAL PROBIOTIC PROPERTIES OF LACTIC ACID BACTERIA AND YEAST STRAINS

ABSTRACT

Four lactic acid bacteria (LAB) and three yeast strains isolated from a traditional Bulgarian cereal-based fermented beverage were assessed for potential probiotic properties. Acid and bile resistance, antipathogenic activity and antibiotic resistance of the strains were evaluated. Tolerance to low pH values (2.0–3.0) and high bile concentrations (0.2–2.0%) of the LAB and yeast strains varied, but all strains kept viable throughout the experiments. Antagonistic activity towards most of the eight test-pathogens was observed for one LAB (Lactobacillus plantarum B28) and two yeast strains (Candida rugosa Y28 and Candida lambica Y30). Antibiotic resistance (39 antibiotics) of the LAB strains was variable, but showed their potential for therapeutic application.     

ASSESSMENT OF POTENTIAL PROBIOTIC PROPERTIES OF LACTIC ACID BACTERIA AND YEAST STRAINS

Four lactic acid bacteria (LAB) and three yeast strains isolated from a traditional Bulgarian cereal-based fermented beverage were assessed for potential probiotic properties. Acid and bile resistance, antipathogenic activity and antibiotic resistance of the strains were evaluated. Tolerance to low pH values (2.0-3.0) and high bile concentrations (0.2-2.0%) of the LAB and yeast strains varied, but all strains kept viable throughout the experiments. Antagonistic activity towards most of the eight test-pathogens was observed for one LAB (Lactobacillus plantarum B28) and two yeast strains (Candida rugosa Y28 and Candida lambica Y30). Antibiotic resistance (39 antibiotics) of the LAB strains was variable, but showed their potential for therapeutic application.     

乳酸发酵鲜菇汁技术研究

以鲜菇汁为原料，经乳酸发酵而制成鲜菇汁发酵饮料，通过对接种量、发酵时间、乳粉量、糖酸比进行试验．从而确定最佳工艺参数为：接种量3％，乳粉量5％，发酵时间3．75h，蔗糖12％，柠檬酸0．04％。     

FUNDAMENTALS AND PERSPECTIVES FOR THE USE OF BACTERIOCINS PRODUCED BY LACTIC ACID BACTERIA IN MEAT PRODUCTS

Bacteriocins of lactic acid bacteria (LAB) are proteinaceous compounds that may present antimicrobial activity towards important foodborne pathogens and spoilage-related microflora. Due to these properties, bacteriocin-producing strains or purified bacteriocins have a great potential of use in biologically based food preservation systems. Despite the growing number of articles describing the isolation of bacteriocinogenic strains, genetic determinants for production, as well as the purification and biochemical characterization of these inhibitory substances, there are only limited reports of successful application of bacteriocins to meats.

This paper presents a critical review of the methods available for screening of bacteriocin-producing LAB strains from meats and also discusses the proposed mechanisms of action for LAB bacteriocins. Additionally, an overview of the Brazilian experience in the application of LAB bacteriocins to meats and meat products is given.     

FUNDAMENTALS AND PERSPECTIVES FOR THE USE OF BACTERIOCINS PRODUCED BY LACTIC ACID BACTERIA IN MEAT PRODUCTS

Bacteriocins of lactic acid bacteria (LAB) are proteinaceous compounds that may present antimicrobial activity towards important foodborne pathogens and spoilage-related microflora. Due to these properties, bacteriocin-producing strains or purified bacteriocins have a great potential of use in biologically based food preservation systems. Despite the growing number of articles describing the isolation of bacteriocinogenic strains, genetic determinants for production, as well as the purification and biochemical characterization of these inhibitory substances, there are only limited reports of successful application of bacteriocins to meats.

This paper presents a critical review of the methods available for screening of bacteriocin-producing LAB strains from meats and also discusses the proposed mechanisms of action for LAB bacteriocins. Additionally, an overview of the Brazilian experience in the application of LAB bacteriocins to meats and meat products is given.     

乳酸菌的固定化方法及其发酵特性的研究

对乳酸菌固定化工艺参数及发酵特性进行了研究,结果表明,乳酸菌包埋法固定最佳工艺条件为:海藻酸钠的浓度为2%,菌体的稀释比例为1:1,CaCl2溶液的浓度为1.5%,菌种的固定化温度为42℃。固定化菌种胶珠含菌量为4.59×109个/g。固定化乳酸菌产酸和耐酸能力强,菌种活力得到保持,具有持久和可重复利用的特点。     

多菌种混合乳酸菌发酵酸豆奶工艺研制

本文研究了市售酸牛奶和自制酸泡菜中分离筛选菌种，确定菌种间的比例。通过对生产过程中的条件进行初步摸索，以鲜豆奶为主要原料，采用多菌种混合液态发酵，可生产出色、香、味俱佳，不舍任何防腐剂的纯天然酸豆奶新产品，从而为工业化生产乳酸菌发酵酸豆奶提供技术依据。