Nutritional Quality and Sensory Acceptability of Akara Prepared from Germinated Tepary Beans

Tepary beans, Phaseolus Acutifolius (ungerminatcd and germinated for 48 hr) were used to prepare akara. Cowpcas, ungerminated and germinated teparies, uncooked and cooked akara prepared from teparies and cowpeas were analyzed for proximate composition, amino acid (AA) profile, vitamin A, ascorbic acid and trypsin inhibitor activity (TIA). Proximate composition and AA profiles of ungerminated teparies and cowpeas were similar. Germination increased protein, vitamin A, and ascorbic acid by 12%, 56%, and 23-fold, respectively. TIA decreased by 2.9% while AA profiles showed slight to no change. Frying lowered protein, vitamin A, ascorbic acid, and TIA by 7%, 35–40%, 75–82%, and 12.6–24.3%, respectively. Taste panels found no significant differences (P≤0.05) in color, texture or flavor among the products.     

Vitamin E constituents of Weaning Foods from Germinated Cereals and Legumes

Four weaning food formulations prepared from 70% 72-hr germinated rice/corn and 30% 48-hr germinated mungbean/cowpea were studied for tocopherol constituents and compared to ungerminated controls. γ-TocopheroIs were the major constituents of the mungbean-supplemented samples. The cowpea-supplemented foods contained a predominance of δ-tocopherols followed closely by γ-tocopherols, α-Tocopherols were comparatively higher in corn- than in rice-formulations. Tocopherols in the weaning foods decreased due to germination especially the γ-tocopherols of germinated corn-mungbean.     

Survival and growth of foodborne pathogens during cooking and storage of oriental-style rice cakes

Fresh cooked rice cakes for retail sale are typically held at room temperature because refrigeration dramatically reduces their quality. Room temperature, high water activity, and a pH of > 4.6 provided an environment conducive to pathogen growth. To date, no studies have been published regarding survival and growth of foodborne pathogens in fresh cooked rice cakes. This study was undertaken to investigate the effect of steam cooking on foodborne pathogens and their subsequent growth in five varieties of rice cakes made from flours of regular rice, sweet rice, white rice, tapioca, and mung bean. Bacillus cereus spores were detected in white rice, tapioca, and mung bean samples. The rice cake flours were inoculated with non-spore-forming foodborne pathogens (Escherichia coli O157:H7, Salmonella enterica serovar Typhimurium, Listeria monocytogenes, and Staphylococcus aureus) or spore-forming bacteria (Bacillus cereus) and steam cooked (100 degrees C) for 30 min. Steam cooking significantly reduced (> 6 log CFU/g) non-spore-forming foodborne pathogens in all samples and inactivated spores of B. cereus by 1 to 2 log CFU/g. Although spores of B. cereus survived steam cooking and germinated during 3 days of storage at room temperature, populations in most rice cakes remained below 106 CFU/g, which is the threshold for producing toxin. Rice cakes made from mung bean flour supported growth and germination of B. cereus spores above that critical level. In mung bean rice cakes, enterotoxin production was detected by the second day, when B cereus cell populations reached about 6.9 log CFU/g. The toxin concentration increased with storage time. However, our results suggest that rapid growth of total mesophilic microorganisms by more than 7 to 8 log CFU/ml during the first day of storage produced off flavors and spoilage before B. cereus was able to grow enough to produce toxins. Therefore, steam-cooked rice cakes made from a variety of flours including mung bean flour are safe for sale for up to 1 day after storage at room temperature and are free of B. cereus toxins.     

Effect of heat-moisture treatment for utilization of germinated brown rice in wheat noodle

Effect of heat-moisture treatment of germinated brown rice (GBR) on the texture and cooking quality of the noodles containing mixtures of wheat and GBR flours was investigated. With the increase in GBR content, hardness and tensile strength of the composite noodles decreased and cooking loss and water absorption increased. Pasting viscosity of the flour mixtures was significantly decreased by increasing the amount of GBR. Heat-moisture treatment of GBR (17 or 20 g/100 g moisture at 100 °C for 4 h), however, apparently increased the pasting viscosity and improved the texture and cooking quality of the composite noodles. The mixture of wheat and a treated GBR (1:1 weight ratio) showed a pasting viscosity similar to pure wheat flour. The noodle containing the heat-moisture treated GBR flours showed the lower cooking loss, and higher hardness and tensile strength than the noodle containing untreated counterparts. Among the treated GBR flours tested, the GBR treated at 100 °C for 4 h at a moisture level of 17 g/100 g was the most acceptable in terms of cooking quality and textural property, close to those of the noodle of pure wheat flour. Substitution with the heat-moisture treated GBR, however, made the noodle darker because of thermal discoloration of GBR.     

Water sorption and cooking time of red kidney beans (Phaseolus vulgaris L.): part I – Effect of freezing and drying conditions on water sorption and cooking time

Water sorption and cooking time of kidney beans were determined. The beans were manually harvested at 19.2 ± 0.1% moisture content and stored at ?20 and ?10 °C for about half a year. The beans were further dried at 30, 40 and 50 °C inside a thin‐layer drier for 7.5 h or under room conditions for 4 week. The freezing storage temperature before the beans were dried did not influence their cooking time and water sorption. The saturated kernel volumes decreased approximately 7% after drying. The beans decreased their sphericity during water sorption and had a larger swelling ratio in the thickness direction than in other directions. Lower initial moisture content, especially with a higher drying temperature, decreased water sorptivity and resulted in higher percentage of uncooked kernels if the beans were not soaked before cooking. However, there was no relationship between initial moisture content and uncooked percentage if the beans were soaked before cooking. High drying temperature resulted in hard‐to‐cook (HTC) phenomenon.     

Rheological properties of flour and sensory characteristics of bread made from germinated chickpea

Breads were prepared from wheat flour supplemented with 0,10,20 and 30% ungerminated or germinated chickpea flour to determine the influence of germination on the sensory acceptability of the baked products. Rheological characteristics of the flours were evaluated by the Brabender farinograph and viscoamylograph, using a wheat flour control. Farinograph development and stability times decreased, and amylograph peak viscosities increased for all fortified flours. Germinated flours showed greater retrogradation upon cooling. Most sensory characteristics of fortified breads did not differ significantly, but the chickpea loaf fortified with 10% germinated flour did not compare favourably with the control.     

Biochemical properties,nutritional quality,colour profile and techno-functional properties of whole grain sourdough and malted cowpea and quinoa flours

The particular interest in biomodifications of underutilised but nutritionally distinct whole grains is vital to promote diet diversity, nutrition transition and food security. This study investigated the use of short-term solid-state fermentation and germination to ease processability and improve the quality characteristics of whole grain (WG) cowpea and quinoa flours. The fermented and germinated WG flours were prepared at 28 °C for 48 h. The biochemical, nutritional quality and techno-functional properties of the obtained flours were determined. The macromolecules’ biomodification by microbial metabolism and endogenous enzymes activation influenced quality variations in the biomodified flours. The cowpea sourdough flour (CSF) presented lower acidity (pH 4.72), higher total flavonoid (29.63 mg QE/g), total phenolic (8.21 mg GAE/g) and antioxidant activity. The flour also showed high contents of fibre (5.30%), ash (4.42%), calcium (864.49 mg/kg), potassium (12848.64 mg/kg), zinc (33.83 mg/kg), good protein (21.43%) and a moderate fat level (2.65%). Higher oil absorption and water solubility indices were also noted for CSF. In contrast, malted quinoa flour (MQF) exhibited higher swelling power, increased dispersibility and improved final, peak and trough viscosities. The CSF displayed higher redness and browning index, whereas MQF had greater lightness. The results suggest that CSF and MQF had the best complementary quality attributes. Their formulation as gluten-free, whole and multigrain ingredients may promote healthy choices for individualised growing dietary needs.     

Effect of germination and dehulling on functional properties of legume flours

The functional properties of flours derived from selected legumes were studied before and after dehulling of the germinated seeds. Ungerminated seeds were used as the control. The chosen legumes were green gram (Phaseolus aureus), cowpea (Vigna catjang), lentil (Lens culinaris) and bengal gram (Cicer arietinum). Dehulled samples had a higher protein solubility compared with germinated and control samples. The bulk densities of germinated and dehulled legume flours were lower compared to control. Germination increased water absorption capacities of legume flours from 1226, 1285, 974 and 1362 g kg^?1 to 1481, 1433, 1448 and 1517 g kg^?1 in green gram, cowpea, lentil and bengal gram, respectively. Fat absorption capacities increased in germinated samples (1130, 1242, 920 and 837 g kg^?1) as against 900, 993, 857 and 788 g kg^?1 at ambient conditions for green gram, cowpea, lentil and bengal gram, respectively. On dehulling, the fat absorption capacities of samples were reduced and the differences were statistically significant. The emulsification capacities of control samples ranged from 55 to 193 ml oil emulsified per gram of sample. On germination and dehulling, the emulsification capacities, activities and stabilities of samples increased significantly. There were increases in foaming capacities and reduction in foam stabilities of all the samples investigated on germination and dehulling. Thus, the study indicated that germination and dehulling improved the functional properties of legumes. Copyright © 2006 Society of Chemical Industry     

Use of Seed Sprouting in Modification of Food Nutrients and Pasting Profile of Tropical Legume Flours

The study investigates the effect of seed sprouting on minerals, anti nutrients and pasting characteristics of flours produced from some tropical legume seeds. Samples were collected from cowpea (Vigna ungulculata); red kidney beans (Phaseolus vulgaris), and Pigeon pea (Cajanus cajan) and each sample was divided into two portions. A portion was sprouted while the other portion served as the control. The flours from sprouted and non-sprouted seeds were analyzed for the minerals, anti nutritional and pasting properties. The samples were found to differ significantly (p < 0.05) in the minerals, anti nutritional composition and pasting properties of the flours. Sprouting increased the mineral contents of the flours generally the calcium content of non-sprouted red kidney beans (13.8 mg/100 g) increased to 16.1 mg/100 g in the sprouted flour. The haemagglutinin decreased from 40.6 HU/mg in non sprouted red kidney to 6.4 HU/mg in the sprouted. The final viscosity of the non sprouted flours were higher than those of the sprouted, with non sprouted cowpea scoring the highest, 272 RVU, while sprouted red kidney beans had the least, 109.06 RVU, which implies that sprouting caused a reduction in the gel strength and elasticity of the samples. Seed sprouting effectively increased the mineral nutrients and lowered the anti nutrients, but produced steamed paste with poor gel strength.     

Utilization of germinated and heat-moisture treated brown rices in sugar-snap cookies

Utilization of germinated brown rice (GBR) in sugar-snap cookies and effect of heat-moisture treatment of the GBR were investigated. Brown rice was germinated at 30 °C for 48 h and then a heat-moisture treatment was conducted for the moistened GBR (17 g/100 g moisture content) at 100 °C for 4 h. Sugar-snap cookies were prepared with white rice, brown rice, GBR and the treated GBR flours, as substitutes for wheat flour (30–100 g/100 g). All cookies containing rice flours, regardless of germination and heat-moisture treatment, required significantly less force to compress than did the wheat flour cookie, and this softening effect was increased as the level of rice flour substitution increased. The cookies made with the GBR flour displayed inferior physical characteristics compared to those with wheat flour, but the cookies containing the treated GBR flour showed improved physical properties with lower moisture content and higher spread factor than those containing untreated GBR flour. The cookies containing the treated GBR flours showed relatively a low degree of firming during the ambient storage. The overall results showed that the cookies with acceptable quality and improved nutrition could be prepared by partial or complete replacement of wheat flour with the heat-moisture treated GBR flour.     

The impact of dehydration process on antinutrients and protein digestibility of some legume flours

Dehydrated foods are specially designed for patients with mastication or/and deglutition problems. This study has assessed the effects of soaking, cooking and industrial dehydration treatments on antinutrient factors and also on protein digestibility in legume flours (chickpea, lentil and bean). A general decline of phytic acid was observed during dehydration, being the most accentuated in case of lentil (44%), followed by white beans and pink-mottled cream beans. Beans were the legumes that showed the highest levels of enzyme inhibitors and lectins, however processing such as cooking and dehydration significantly reduced (p < 0.05) their levels further to negligible concentrations. The dehydration did not cause further effects than ordinary cooking in reduction of the concentration of polyphenolic compounds of flours. However, a higher increase of in vitro protein digestibility (IVPD) was produced by dehydration in all legumes from 12% to 15%. Thus, dehydrated legume flours could be considered ready-to-use for special meals to specific populations.     

Comparison of chemical compositions and bioactive compounds of germinated rough rice and brown rice

The aim of the study was to compare changes in the chemical compositions and bioactive compounds of germinated rough rice and germinated brown rice. Ungerminated rice (brown rice) and germinated rice extract powder were also prepared, for comparison purposes. In general, the concentration of crude protein, total free amino acids, α-tocopherol, γ-oryzanol, thiamine, niacin and pyridoxine, in the germinated rough rice and the germinated rice extracted powder, were significantly higher, than those of the germinated brown rice and the ungerminated rice, whilst there was no significant difference in the levels of crude fat, carbohydrate and ash. The amino acid contents of the germinated rice products were also investigated and differences were found amongst these samples. The most significant changes, in γ-aminobutyric acid, glycine, lysine and leucine, were observed in the germinated rough rice and the germinated rice extracted powder.     

Efficiency of some technological processes on reducing the residues of malathion and pirimiphos methyl in mature broad bean seeds

Residue study was performed on several insecticides which could contaminate local Egyptian beans. The effect of storage periods and various processing steps on lowering the residues of malathion and pirimiphos methyl in treated seeds and their processed products were investigated. The data indicated that malathion and pirimiphos methyl persisted for more than 90 days on and in stored mature dry broad beans after postharvest treatment. However, stored broad beans could be safely used for human consumption after 90 days when the insecticide residues reached safe levels. Washing removed 69 and 75% of malathion and pirimiphos methyl residues of treated broad beans, respectively. Malathion residue was not detected in various processed products. More than 89 and 99% of malathion residues were absent in dehulled and heated dehulled broad beans. In addition, pirimiphos methyl residues were reduced to 92, 97, 87, 99, 99, and 95% from the initial levels in treated beans following dehulling, cooking of dehulled beans, germination, cooking of germinated beans and cooking of the beans by the common method and under pressure, respectively.     

豌豆全粉对小麦面团特性和面包品质的影响

探讨了萌芽程度不同的豌豆全粉(添加量均为5%)对小麦面团加工特性(水分分布、黏性、发酵流变特性、动态流变特性)及面包品质(面包芯质构、面包贮藏期硬度等)的影响。结果表明:与小麦面团(WD)相比,添加未萌芽豌豆全粉(G0MD)的面团中不易流动水的比例升高,结合水和自由水的比例降低,面团发酵产气量降低,面团储能模量(G′),损耗模量(G″)显著下降,面包在储藏期内(1～7 d)面包的硬度增加缓慢。与G0MD相比,添加萌芽豌豆全粉的面团中结合水比例增加,不易流动水比例降低,黏性显著降低,G′、G″均增加,面包老化速度变慢。因此,将萌芽豌豆全粉用于面包的制作表现出良好的应用前景。     

Influence of germination on the nutritional quality of Phaseolus vulgaris and Cajanus cajan

Germination is a method that can modify the presence of nutrients and antinutrients in legume seeds. In this study, the changes in trypsin inhibitors activity (TIA), phytic acid, tannins, ascorbic acid, thiamine, protein digestibility and minerals in germinated black beans (Phaseolus vulgaris L.), white beans (Phaseolus vulgaris L.) and pigeon beans (Cajanus cajan L. Millsp) were evaluated. The ungerminated grains were analysed as control. A significant decrease in the content of TIA of pigeon beans (19.2%), white beans (52.5%) and black beans (25%) was observed. The reduction of phytic acid was more than 40% for the three grains germinated; for the tannins, the reduction was of 14.3% for pigeon beans, 19% for black beans and 36.2% for white beans. Germination increased the protein digestibility in a 2-4% range, and also increased the ascorbic acid by 300% for white beans, by 33% for black beans and by 208% for pigeon beans. The thiamine content increased more than 26.7%. Germination affected the mineral content erratically, depending more on the grain and the type of mineral. These variations in the content of nutrients and antinutrients of the germinated grains are attributed to the joint effect of the germination and previous soaking the grains were subjected to.     

Influence of grain germination on functional properties of sorghum flour

A Sudanese sorghum cultivar (Fetarita) was germinated for five days and protease and amylase activities were measured every 24 h. Results showed that flour obtained from the 3rd germinated sorghum grain had high protease and amylase activities. The functional properties of flours derived from the germinated sorghum seeds were studied and ungerminated seeds were used as a control. Germinated samples had a higher protein solubility compared with the control, and the highest solubility occurred at pH 6. Germination also increased the protein solubility index of sorghum flour. Germinated sorghum flour had a least gelation concentration of 8% compared with 18% for the control. The bulk densities of germinated flours were lower compared to the ungerminated one. Water and oil capacities were increased by germination from 131.34% and 90.56% to 141.64% and 108%, respectively after three days of germination. The emulsifying activities and stabilities of the germinated samples increased significantly. In addition, germination improved the foamability of sorghum flour from unfoam flour to a flour with foam after three days of germination; and the foaming capacity and stability increased significantly with increasing germination time. Thus, the study indicated that germination improved the functional properties of sorghum and it would be possible to design new foods using germinated sorghum.     

糙米发芽过程中内源酶活力及主要成分的变化

目的：探讨糙米在发芽过程中内源酶活力和主要成分的变化规律及相关性。方法：以“早944”稻米为材料制备发芽糙米,在30℃下发芽培养72h,每隔12h对其内源酶活力和主要成分含量进行测定。结果：糙米内α-淀粉酶、β-淀粉酶、蛋白酶、谷氨酸脱羧酶、植酸酶等内源酶活力呈现上升趋势,发芽72h时酶活力分别达5.45U/g、3.78U/g、14.08U/g、9.72U/100g、3.44U/g;γ-氨基丁酸、还原糖、游离氨基酸含量呈现增长趋势,发芽72h时含量分别为237.05mg/100g、18.15mg/g、214.94μg/g;同时,总糖、粗蛋白和植酸含量呈现降低趋势,发芽72h时含量分别为44.16、9.59、1.99mg/g。结论：内源酶与其代谢的底物及代谢产物之间都存在显著相关性,部分内源酶与非内源酶代谢相关底物及产物之间亦存在显著相关性。糙米发芽72h后的营养价值高于发芽前,适宜用作新型糙米食品的生产原料。     

Effects of germination time on the structural,physicochemical and functional properties of brown rice

Germinated brown rice (GBR) is a gluten-free food raw material. Its tissue structure, physicochemical properties and functional properties depend on the germination time. In this study, rapid viscosity analyses, differential scanning calorimetry and X-ray diffraction et al were used to analyse the structural, physicochemical and functional changes in brown rice (BR) during germination. Gamma-aminobutyric acid (GABA), total phenolic compound, glutathione (GSH) and amino acid contents increased during germination. GBR exhibited the highest GSH (22.70 mg per 100 g) and amino acid (8.02 mg per 100 g) contents at 24 h and the highest GABA content (253.35 mg per 100 g) at 36 h. Furthermore, BR germinated for 36 h showed greater enthalpy (ΔH) than ungerminated BR. Although GBR showed less crystallinity than ungerminated BR, germination did not change the crystalline structure type of starch (A-type). These results inform choices of the appropriate applications of GBR to promote its utilisation in the food industry.     

Changes in phytate and minerals during germination and cooking of fenugreek seeds

The effects of germination, cooking and roasting on the phytic acid content, total phosphorus, water soluble inorganic phosphorus and mineral content of fenugreek seeds have been estimated. After 96 h germination, the dry weight of fenugreek seeds decreased while total ash content increased. Phytase and phosphatase activity of the ungerminated and germinated seeds have been assayed. It is observed that during germination the phytic acid values diminish and the water soluble inorganic phosphorus values increase. Phytase activity which is absent in the ungerminated seeds originates after germination and the phosphatase activity is increased in the germinated seeds. Heat treatment practised in cooking and roasting fenugreek seeds has less effect on phytate elimination than does germination. Changes in calcium, magnesium, iron, manganese, copper and zinc are found to be dependent on the loss of dry weight which occurs during processing of fenugreek seeds.     

发芽糙米的富硒及其微波干燥与挤压膨化工艺优化

以普通粳稻为原料,探讨了发芽糙米的富硒效果和微波干燥、挤压膨化对富硒发芽糙米营养品质的影响。结果发现,硒质量浓度为10 mg/L时,可以获得较高质量的富硒发芽糙米,此条件下糙米的发芽率为97.9%,有机硒含量为977.6 μg/kg（质量分数98.5%）,γ-氨基丁酸含量为445.9 mg/kg;40 ℃的低温微波干燥有利于保持发芽糙米的硒和γ-氨基丁酸含量;挤压膨化产品中有机硒和γ-氨基丁酸的含量与原糙米相比,分别提高到其29 倍和5 倍。研究认为,亚硒酸钠可以作为富硒试剂实现发芽糙米的有效富硒,富硒发芽糙米可以用于开发相关的营养膨化食品。