乳酸菌发酵对燕麦淀粉物化及热力学特性的影响

以燕麦粉为原料,分别利用植物乳杆菌(L.p)和旧金山乳杆菌(L.s)两种乳酸菌对其进行发酵,研究燕麦淀粉在发酵过程中各种物化及热力学特性的变化。结果表明：燕麦粉经过两种菌发酵后pH 值下降,且L.p 发酵的燕麦粉pH 值下降的速率和产酸量都大于L.s。但是到发酵后期,两种乳酸菌发酵的燕麦粉pH 值相近。发酵燕麦淀粉的溶解度和溶胀力都随着温度的升高而增加,在不同温度下,其溶胀力和溶解度在发酵过程中的变化趋势不同,经L.P 发酵的样品的溶胀力低于L.s,但溶解度大于经L.s 发酵的样品。快速黏度分析仪(RVA)和差示扫描量热仪(DSC)分析得到发酵过程中燕麦淀粉的变化：发酵后燕麦淀粉糊化过程中峰值黏度随着发酵时间的延长而降低,糊化起始温度提前,糊化所需时间延长,糊化焓升高,且L.s 发酵样品的糊化焓值高于L.p。发酵后燕麦淀粉的直链淀粉含量在发酵过程中呈上升趋势,L.p 发酵的样品的直链淀粉的含量高于L.s。     

自然发酵对豌豆淀粉理化性质的影响

豌豆在35℃下分别自然发酵0、1、3、5 d,研究对豌豆淀粉的直链淀粉含量、透明度、溶解度、膨润力、黏度特性和质构特性等理化性质的影响。结果表明:随着发酵时间的延长,发酵液的pH从7下降到4.2,直链淀粉含量增加,透明度降低。自然发酵后豌豆淀粉的溶解度和膨润力均降低,发酵5 d后,糊化温度从73.05℃升高到83.55℃,峰值黏度从178.42 RVU降低到120.8 RVU,淀粉凝胶硬度从920.34 g降低到104.01 g。     

Physicochemical Characterization of Selected Sweet Potatocultivars and Their Starches

The uses of sweet potato tubers rely on the physicochemical and functional properties of its starch. The different properties studied from 14 lines include cooking quality, extractable starch, dry matter, starch, sugar, carotenoids, and anthocyanin. The properties of extracted starch like swelling volume, solubility, amylose content, clarity of paste, rheological properties were examined. The organic matter content of different lines almost remained the same. Starch content of the tubers was greater than 20% and the sugar content ranged from 1–3%. The extractable starch was in the range of 16–21%. The anthocyanin content was high for ST- 13, while ST- 14 was rich in carotene. The swelling volume was comparatively low and the solubility ranged from 8.81–13.65%. Most of the starches examined in this present study displayed excellent viscosity properties with peak viscosity greater than 3000 cP. This study revealed that many lines have excellent potential for use as nutritive and calorific food and as industrial raw material.     

Functional properties of the starchy flour extracted from cassava on fermentation with a mixed culture inoculum

The functional properties of the starchy flour extracted from six varieties of cassava subjected to fermentation by a mixed culture inoculum comprised of lactobacilli, streptococci, corynebacteria and yeast cells are examined. Apparent reduction in total and soluble amylose contents is observed. Differential scanning calorimetry of the samples indicated that the enthalpy of gelatinisation was reduced, while the gelatinisation temperature was enhanced. A marked reduction in Brabender viscosity values of starch from fermented tuber was observed, but the X-ray diffraction pattern was unaffected. All these changes were attributed to the presence of fibrous material and consequent reduction of starch in unit volume rather than any change in the starch granule structure.     

Physicochemical Changes in Cassava Starch and Flour Associated With Fermentation: Effect on Textural Properties

Physicochochemical changes in cassava starch and flour associated with fermentation were investigated and related to textural properties of its flour pastes. Cyanide and pH decreased, while crude protein, acidity, and apparent amylose content increased in the fermented products. Average starch granule diameter, solubility, and swelling power were depressed, while gelatinization enthalpy increased. Amylograph of starch showed increased peak visocity temperature, reduced peak, breakdown, and setback viscosities, while Texture Profile Analysis showed a decreased in hardness, cohesiveness, elasticity, and gumminess of the fermented flour paste. The altered textural properties were attributed to greater starch granule stability due to short amylose-like fragments formed by enzymatic hydrolysis of amylopectin.     

蕉藕淀粉与薯类淀粉特性对比研究

为探索蕉藕淀粉应用范围,选取木薯淀粉、马铃薯淀粉、红薯淀粉等薯类淀粉为对照,对蕉藕淀粉与常见薯类淀粉的组成、吸水(油)率、溶解度、膨胀度、透明度、凝沉性、凝胶强度、冻融稳定性及黏度等特性进行了对比分析。结果显示:蕉藕淀粉与常见薯类淀粉特性相比除吸水性差异不显著外,吸油率、溶解度、膨胀度、透明度、凝沉性、凝胶强度、冻融稳定性及黏度变化均存在显著差异,可利用蕉藕淀粉直链淀粉含量高、透明度好、凝胶强度高等特性,开发新型淀粉食品和增稠稳定剂。     

干热处理对藜麦淀粉结构和理化性质的影响

以高筋小麦粉为原料添加酵母制作面团,在温度28℃、湿度80%条件下发酵1～6 h,提取小麦淀粉。研究发酵时间对小麦直链淀粉含量、损伤淀粉含量、粒径、颗粒表面结构、糊化及消化特性的影响。结果表明：面团发酵1 h后直链淀粉含量和损伤淀粉含量均显著低于原淀粉(P<0.05)。快速黏度分析结果表明,发酵1 h的小麦淀粉峰值黏度、热浆黏度和冷浆黏度最低。发酵处理后淀粉的粒径均小于原淀粉,其中发酵5 h的平均粒径最小。扫描电镜结果表明,发酵6 h的小麦淀粉颗粒表面出现破裂现象。体外消化实验表明,发酵5 h和6 h小麦淀粉的消化速率最高,发酵后抗性淀粉含量低于原淀粉,而慢消化淀粉含量高于原淀粉(P<0.05)。     

Physicochemical, pasting and thermal properties of tuber starches as modified by guar gum and locust bean gum

This study was carried out in order to compare the functional characteristics of isolated starch from five tuber crops, yam, taro, sweet potato, yam bean and potato, as well as effect of guar gum (GG) and locust bean gum (LBG) on pasting and thermal properties of tuber starches. The results showed that total amylose content of five tested starches ranged from 17.85% to 30.36%. The results of pasting behaviour showed that potato starches exhibited the highest peak viscosity and yam starch presented a stable curve with little breakdown viscosity. Addition of GG and LBG resulted in a significant increase in peak, final viscosity, breakdown and setback viscosity for all tuber starches ( P  < 0.05), but a slight decrease in pasting temperature. The gelatinisation enthalpy (Δ H ) for starches with GG and LBG was slightly lower than those of the starches alone in yam and sweet potato, but not in taro and yam bean.     

面团发酵过程中小麦淀粉理化性质的变化

以小麦粉为原料,结合水洗和离心提取小麦淀粉,研究面团发酵过程中一次发酵、二次发酵和醒发工序对直链淀粉含量、溶解度、溶胀度、糊化特性和凝胶特性等理化性质的影响。试验结果表明:发酵后直链淀粉质量分数提高3.7%～8.18%;采用黏度速测仪(RVA,Rapid visco analyzer)分析醒发后小麦淀粉的峰值黏度、谷值黏度和最终黏度分别上升52 RVU、58.67 RVU和73.33 RVU;采用质构仪(TPA,Texture profile anal-ysis)对发酵过程小麦淀粉凝胶特性进行研究,发现醒发后凝胶特性有所改善,硬度提高48.60 g,弹性提高0.03,内聚性、回复性分别提高0.05、0.15。     

Comparisons of physicochemical properties and antioxidant activities among pumpkin (Cucurbita moschata L.) flour and isolated starches from fresh pumpkin or flour

Physicochemical properties (pasting and thermal properties, swelling power, water solubility and antioxidant activities) and chemical composition of pumpkin flour (PF) and starches, isolated from fresh pumpkin (SFF) or flour (SFP) were compared. SFP and SFF had similar proximate composition and amylose content. Drying process during PF preparation modified starch properties. PF exhibited greater antioxidant activities (DPPH, ABTS and FRAP), but had lower β‐carotene than fresh pumpkin. SFF and SFP had higher amylose content than PF, and exhibited a B‐type X‐ray diffraction pattern with spherical and dome‐like starch granules. Peak viscosity of SFP was relatively higher than that of SFF, probably due to lower swelling power and water solubility, which indicated increased granular rigidity in the SFP starch structure. Compared with SFF and SFP, PF had significantly higher onset (T_o), peak (T_p) and conclusion (T_c) temperatures due to a lower starch content and relatively higher fibre content, which resulted in lower gelatinisation enthalpy (ΔH).     

Extraction,Physicochemical and Compaction Properties of Tacca Starch — a Potential Pharmaceutical Excipient

Some physicochemical properties of tacca starch (Tacca leontopetaloides, Taccaceae) have been examined and the results compared to those of maize and potato starches. Tacca starch was found to have a higher amylose content than maize starch but a lower content than potato starch. The starch granules were small (average particle size 3.5 μm) relative to maize and potato starches and were predominantly polyhedral with edges. The gelatinisation characteristics except from the temperature were similar to those of maize starch but much higher than those of potato starch. Tacca starch had relatively higher swelling power and solubility than the other starches. Its features in the formation of compacts (tablets) were comparable to those of maize starch with tacca starch being more resistant to deformation.     

Development and Testing of Methods to Screen Chickpea Flour for Starch Characteristics

Rapid screening methodology has been developed for assessing the chemical composition and the gelatinisation and pasting properties of starch using flour from chickpea seeds. The methodology allows samples to be assessed using a minimal amount of sample and for starch information to be interpreted in the presence of other components present in the flour. The starch content of the flour and the amylose content of the starch was determined by modifying existing kit-based methods (Megazyme International Ltd). Using the Differential Scanning Calorimeter (DSC), starch gelatinisation characteristics can be assessed based on temperature of gelatinisation (T_p), specific heat capacity (Cp^sp ) and half width of transition (½ΔT). Pasting properties could be assessed using the Rapid Visco Analyser (RVA), based on the determination of onset temperature for the RVA profile (T \displaystyle _o^{RVA} ), T_p − T \displaystyle _o^{RVA} and final viscosity (FV) values. The developed methodology was tested using a range of chickpea samples. No significant variation was found between the samples for starch content or for the proportion of amylose/amylopectin in the various starches. Significant variation was found in the starch properties of two of the samples, while the variation between the remaining samples was very small.     

芡实粉和芡实淀粉理化性质和消化性研究

以芡实为原料制取芡实粉和芡实淀粉样品,研究了其糊化性、溶胀度、可溶指数、质构性和消化性等性质.结果表明,芡实淀粉样品的黏度明显高于芡实粉样品,芡实粉的峰值黏度与芡实淀粉相比降低了57.75％;随温度升高,芡实粉和芡实淀粉的溶胀度和可溶指数呈现递增趋势,但在较高温度下,芡实粉样品的溶胀度和可溶指数明显高于芡实淀粉样品;与芡实淀粉样品相比,芡实粉的凝胶的硬度减少了91.86％;芡实淀粉样品的快速消化淀粉含量明显高于芡实粉样品,但慢速消化淀粉的含量要低于芡实粉样品,抗性淀粉的含量高于芡实淀粉样品.     

Fermentation by Amylolytic Lactic Acid Bacteria and Consequences for Starch Digestibility of Plantain, Breadfruit, and Sweet Potato Flours

The potential of tropical starchy plants such as plantain (Musa paradisiaca), breadfruit (Artocarpus communis), and sweet potato (Ipomoea batatas) for the development of new fermented foods was investigated by exploiting the capacity of some lactic acid bacteria to hydrolyze starch. The amylolytic lactic acid bacteria (ALAB) Lactobacillus plantarum A6 and Lactobacillus fermentum Ogi E1 were able to change the consistency of thick sticky gelatinized slurries of these starchy fruits and tubers into semiliquid to liquid products. Consequently, a decrease in apparent viscosity and an increase in Bostwick flow were observed. These changes and the production of maltooligosaccharides confirmed starch hydrolysis. Sucrose in sweet potato was not fermented by strain A6 and poorly fermented by strain Ogi E1, suggesting possible inhibition of sucrose fermentation. In all 3 starchy plants, rapidly digestible starch (RDS) was higher than slowly digestible starch (SDS) and resistant starch (RS) represented between 17% and 30% dry matter (DM). The digestibility of plantain was not affected by fermentation, whereas the RDS content of breadfruit and sweet potato decreased and the RS content increased after fermentation. Practical Application: The characteristics resulting from different combinations of gluten free starchy plants (plantain, breadfruit, sweet potato) and amylolytic lactic acid bacteria (ALAB) offer opportunities to develop new functional fermented beverages, mainly for breadfruit and sweet potato, after further investigation of their formulation, sensory attributes, nutritional, and prebiotic characteristics.     

Changes in characteristics of sweet potato flour prepared by different drying techniques

The changes occurring in the characteristics of sweet potato flour as a result of processing were investigated. Pasting behaviour of drum dried and hot air-dried sweet potato flour was determined using Rapid Visco-Analyser. The pasting characteristics decreased due to gelatinization of starch during processing. The degradation of starch by amylases during hot air drying further lowered the water binding capacity/viscosity and total amylose and increased the digestibility compared to those of drum dried and native flour. Swelling power and solubility of the flours increased as a result of processing which subsequently increased with increase in temperature. Scanning electron micrographs of starch granules showed tendency of clustering, especially in drum dried samples. X-ray diffraction patterns showed alteration from Ca-type to V-type with a marked reduction in crystallinity index as a result of processing. The ¹³C NMR spectra of processed starches showed reduced peak intensities and line widths due to depolymerizing effects, and also pointing to their change in crystallinity.     

Physicochemical Properties of Sweet Potato Flour and Starch as Affected by Blanching and Processing

The effects of blanching on physicochemical properties of flours and starches prepared from two varieties of sweet potatoes (Mun‐Kai and Negro) were studied and compared. The pasting temperature and peak viscosity of starches, respectively, were 74 and 80 °C and 381 and 433 RVU. The pasting temperature (74.0‐94.8 °C) of flours was greater than that of starch, depending on the variety and blanching process. However, the peak viscosity (ca. 103‐120 RVU) of flours was lower than that of the corresponding starches. Partial gelatinization of starch granules was observed as a result of a 1‐min blanching. Composition of starch and flour was found to affect swelling power and solubility. The starch content of starches, flours from unblanched sweet potato and flours from 1‐min blanched sweet potatoes were 97; 66.3 and 74.9; as well as 36.6 and 40.4%, respectively. Amylose content of flours and starches varied from 17.2‐20.8%.     

基于模糊数学综合感官评价的甘薯淀粉面包的工艺优化

为了提高面包营养价值,改善滋味,降低面包老化速度,延长保质期,将甘薯淀粉添加到面包当中,采用单因素和正交实验,考察配方(甘薯淀粉、黄油、干酵母的添加量)及加工工艺(一次醒发时间、醒发温度和烘焙时间)对面包感官性状和质构的影响。基于模糊数学的评价方法评价甘薯淀粉面包的品质,对甘薯淀粉面包的形状、色泽、松软度、香味、滋味进行感官性质的分析。结果表明:最佳配方为混合粉(小麦粉+甘薯淀粉)150 g,甘薯淀粉的含量为15 g、黄油15 g、干酵母5.5 g;最优加工工艺为一次醒发时间为60 min、醒发温度为25 ℃、烘焙时间为9 min;最优条件下其硬度为27.95 N,弹性为15.18 mm,咀嚼性为228.30 mJ,胶粘性为15.05 N。按此条件制作的甘薯淀粉面包色泽金黄,外形饱满,柔软适口具有甘薯淀粉面包特有的风味,为甘薯淀粉面包工业化生产提供理论指导,同时丰富面包种类,拓宽甘薯淀粉用途。     

Starch from Alberta potatoes: wet-isolation and some physicochemical properties

Starch content of six varieties of Alberta-grown potato tubers was more than 16% (wet basis). The tubers were wet fractionated in the laboratory and starch was recovered. The starch recovery ranged from 61 to 67%. The composition of the potato juice/fruit water (dry matter, protein, ash and mineral content) and starch isolations (moisture, starch, protein, lipid, ash, phosphorous and amylose content) was determined. The physicochemical characterization of the potato starch isolations were done in terms of granule size distribution, crystallinity (X-ray diffractometry), gelatinization (differential scanning calorimetry) properties, swelling factor and the changes in viscosity during heating and cooling of starch-water slurry (Brabender viscography). Substantial differences in the starch content of the tubers, fruit water composition and starch physicochemical properties were observed between potato varieties.     

常用淀粉对甘薯粉糊化特性的影响

利用Brabender粘度仪考察了几种常用淀粉对甘薯粉糊化特性的影响，认为马铃薯、甘薯淀粉使甘薯粉糊化温度降低，玉米、木薯淀粉则使其升高；甘薯、玉米淀粉使甘薯粉糊化时间延长。添加各种淀粉均可使甘薯粉的峰谷粘度提高；马铃薯、玉米淀粉还可大幅度提高其峰值粘度。但马铃薯淀粉粘度破损值大，玉米淀粉破损值小、峰谷粘度高。     

Effect of Processing on the Physico-Chemical Characteristics of Quinoa Flour (Chenopodium quinoa,Willd)

The nature and extent of the modification of starch in quinoa, caused by various processes like cooking and autoclaving of the seeds, drum drying of the flour and extrusion of the grits was investigated by measuring the physico-chemical properties - the water absorption, water solubility, swelling power, viscosity and degree of gelatinisation - of the processed samples. Autoclaved samples showed the lowest degree of gelatinisation (32.5% by DSC) of the starch, while the degree of gelatinisation of the precooked/drum dried samples was found to be 97.4% by DSC method. Higher polymer degradation was observed in cooked samples than in autoclaved samples as seen in the gel chromatographic separation. The water solubility in cooked samples (5.44 to 15.58) and in autoclaved samples (7.02 to 9.64) increased with the process time. In the extrusion process, the moisture content as well as the compression ratio was found to affect the degree of starch modification significantly (p <0.01).