Enzymatic hydrolysis of blue whiting (Micromesistius poutassou); functional and bioactive properties

Functional and biochemical properties of fish protein hydrolysates (FPH) from blue whiting (BW) were studied. FPH (2.5%, 5%, 10%, and 15% degree of hydrolysis [DH]) were made from isolated proteins from headed and gutted BW with Alcalase 2.4 L. The properties of dried BW mince and protein isolate compared to 4 reference proteins (soy and milk protein) were studied: color, solubility, water-holding capacity (WHC), oil-binding capacity (OBC), emulsion capacity (EC), and emulsion stability (ES). The angiotensin I-converting enzyme (ACE) inhibitory activities of the soluble fraction of BW powders were also investigated. Furthermore, the products were characterized by analyzing their chemical composition. Chemical composition, solubility, OBC, and EC of the BW powders was significantly (P < 0.05) different with different DH, while color, ES, and WHC were not significantly (P > 0.05) different. Salt content of the FPH was high (4% to 19%) and increased with increased DH. Protein solubility varied from 10% to 70% and increased with increased DH. WHC of the FPH was around 97% and was higher than that of all the reference proteins tested. OBC decreased with increased DH (from 3.5 to 2.1 g oil/g protein) and was higher than OBC of the soy and milk proteins (1.6 to 1.9 g oil/g protein). EC of FPH was similar or lower than the reference proteins. ES of FPH (60% to 90%) was similar to or lower than soy and whey proteins (60% to 98%) but higher than casein (20%). ACE inhibition activity increased as DH was increased. Practical Application: The results from this study demonstrate that a functional bioactive hydrolysate can be produced from BW, which is an underutilized fish species, and may aid the industry in better utilizing this raw material. The novelty of this research was the use of BW as a raw material where the protein has been isolated with the pH shift method. Furthermore, it was novel that bioactivity and functionality was measured in the same samples.     

乳酸链球菌素和纳他霉素在月饼保鲜中的应用

研究了乳酸链球菌素和纳他霉素对蛋黄月饼的防腐保鲜效果,在蛋黄月饼的蛋黄馅中添加乳酸链球菌素和纳他霉素的复配混合液以及在月饼表皮中添加适量浓度的纳他霉素,具有明显的抑菌保鲜效果,可使蛋黄月饼的保质期延长到45天.     

乳酸链球菌素协同纳他霉素在豆腐保鲜中的应用

以豆腐为研究对象,通过添加乳酸链球菌素（nisin）和纳他霉素以提高豆腐的保鲜时间,以感官评分和菌落总数为评价指标,考察了乳酸链球菌素与纳他霉素添加量、杀菌温度和杀菌时间等对其的影响,通过正交试验得出最佳的优化条件为：nisin与纳他霉素添加量0.12 g/kg、杀菌温度90 ℃和杀菌时间20 min。此工艺条件处理后,冷却并在37 ℃恒温培养箱中保存7 d,测得试样的感官评分为93分,菌落总数为0.53×104 CFU/g。没有添加乳酸链球菌素和纳他霉素的对照组在同样条件下保存1 d,测得感官评分在50分以下,这为延长豆腐的保质期提供了实验基础和理论依据。     

A comparative study of consumer preference for blue whiting (Micromesistius poutassou) and whiting (Merlangius marlangus) before and after incorporation into products

Good quality blue whiting and whiting fillets were compared by taste panels before and after incorporation into five products. In the plain cooked form, blue whiting was liked by assessors but whiting was preferred marginally. In product form, both fish were well liked and there was no significant preference for either species.     

Lipid and protein changes during the ensilage of blue whiting (Micromesistius poutassou Risso) by acid and biological methods

Fish waste is a potential source of protein for animal nutrition. Ensilage could provide an advantageous means of upgrading these residues. Careful control of the degree of proteolysis and lipid oxidation is required to produce silages of high nutritional value. This paper studies the changes in lipids and protein during storage (15 days) of acid silages (with 0, 0.25 and 0.43%, w/w, of formaldehyde) and biological silages (with 10 and 20% molasses or dehydrated whey) prepared from blue whiting. A remarkable reduction in protein solubilisation values was achieved by adding formaldehyde. However, formaldehyde addition led to an increase in the peroxide value of the oil extracted from the silages. Ensiling by biological methods seems promising. It yielded both a considerable reduction in protein solubilisation and in basic volatile nitrogen when compared with acid ensilage. In addition, the oil from biological silages had lower peroxide values than the oil from acid silages with added formaldehyde.     

Frozen storage of high-pressure- and heat-induced gels of blue whiting (Micromesistius poutassou) muscle: rheological, chemical and ultrastructure studies

This paper examines the influence of frozen storage over 34 weeks on the rheological properties as well as the chemical and microstructural characteristics of gels made from muscle of blue whiting (Micromesistius poutassou) subjected to different gelling treatments entailing three combinations of pressure, temperature and time: 200 MPa, <10°C, 10 min (lot L), 375 MPa, 38°C, 20 min (lot H) and atmospheric pressure, 37°C, 30 min and then 90°C, 50 min (lot T). Freezing at –40°C caused certain changes in rheological parameters. In heat-induced gels, breaking deformation, elasticity and cohesiveness increased. Of the high-pressure-induced gels, breaking force increased and cohesiveness decreased in the gel formed at lower pressures, while the only change in the gel formed at higher pressure was some loss of elasticity. There was a general fall in water holding capacity (WHC) values. Lightness remained stable. In terms of protein solubility, there was an increase in covalent bonds in lot L. As for the ultrastructure, all gels matrixes were more disorganized as a result of freezing. In the course of frozen storage, the greatest changes in rheological parameters generally took place during the first 8 weeks, and in all the gels there was a decrease in WHC. In the heat-induced gel the changes were less marked over the storage period compared with those in the high-pressure-induced gels, but the heat-induced gel was more brittle and did not maintain maximum folding test scores. Covalent bonds increased and hydrophobic interactions decreased in all lots. The general appearance of the structure of gel T remained more homogeneous, while the pressurized gels exhibited more and larger cavities.     

In vitro and in vivo evidence for a satiating effect of fish protein hydrolysate obtained from blue whiting (Micromesistius poutassou) muscle

To find appetite suppressive molecules derived from fish protein hydrolysates, both in vitro and in vivo experiments were performed in order to demonstrate that hydrolysates produced from blue whiting muscle (BWMH) possess satiating properties. Here we demonstrated for the first time that a protein hydrolysate obtained from marine source was able to enhance cholecystokinin (CCK) and glucagon-like peptide-1 (GLP-1) secretion in STC-1 cell line. To demonstrate that these in vitro activities also exist in vivo, we investigated the effect of BWMH preload administration in rats and its repercussion on food intake and metabolic plasma marker levels. Results showed that BWMH reduced the short term food intake which was correlated to an increase in the CCK and GLP-1 plasma levels. Moreover it was demonstrated that the chronic administration of BWMH led to a decrease in the body weight gain.     

纳他霉素在气调保鲜草莓中的应用研究

采用10、20mg/L和30mg/L的纳他霉素对草莓果实进行处理后,按30%O2+6%CO2密封包装,在(2±1)℃条件下贮藏。结果表明:纳他霉素处理可有效控制果实表面霉菌数量,减慢叶柄叶绿素含量下降的速率,抑制果实有机酸与糖分的下降,较好地维持果实的营养,延长贮藏期,改善其品质。20mg/L和30mg/L的纳他霉素处理草莓后贮藏21d,腐烂率仅3.3%,品质优良。     

Effect of a two‐step natural organic acid treatment on microbial activity and lipid damage during blue whiting (Micromesistius poutassou) chilling

Quality loss inhibition in chilled blue whiting (Micromesistius poutassou) was investigated. For it, a natural organic acid‐mixture including ascorbic, citric and lactic acids was applied in a two‐step processing strategy: (i) as an aqueous dipping medium previous to chilling storage and, (ii) included in the flake ice employed as chilling system. As a result of the acid‐mixture addition, a partial inhibition of microbial and biochemical mechanisms involved in the quality loss was obtained. Thus, aerobe and psychrotrophe counts in treated fish showed lower ranges (2.07–4.15 and 2.43–4.39 log CFU g^?1, respectively) than control fish (2.64–5.83 and 2.30–5.30 log CFU g^?1, respectively). Sensory analysis revealed that treated fish was still acceptable at the end of the experiment (day 9), while control fish was rejected at this time. Lipid hydrolysis (free fatty acid formation) proved to be more limiting of fish quality than lipid oxidation (peroxide and thiobarbituric acid‐reactive substance formation).     

Chilled storage of high pressure and heat-induced gels of blue whithing (Micromesistius poutassou) muscle

 Microbiological, rheological and chemical characteristics were examined in gels made from the muscle of blue whiting (Micromesistius poutassou) and subjected to three different combinations of pressure, temperature and time: 200 MPa at 3  °C for 10 min (lot L), 375 MPa at 38  °C for 20 min (lot H) and atmospheric pressure at 37  °C for 30 min followed by 90  °C for 50 min (lot T), and kept in chilled storage for 20 days. The microorganism content dropped at the outset as pressurizing took effect, the highest microbial content being found in lot L; however as the effect was not lethal, the total load increased over the following days. Microbial load was significantly lower in lot T. During chilled storage, the values of breaking deformation, breaking force and cohesiveness of lot L were higher than those of the other lots, although they did decrease with storage. The heat-induced gel was much harder, had greater water-holding capacity and was considerably more stable than the high-pressure-induced gels. The lightness value was higher in lot H than in the other two lots. In general, changes in protein solubility tended towards the cleavage of strong bonds as a result of microbial action. The electrophoretic profiles evolved differently in each of the lots over the chilled storage period. However, all of them exhibited large numbers of bands of lower molecular mass which could have been the result of degradation. This was particularly evident in lot H. The heat-induced gels exhibited a highly porous ultrastructure, quite different from the high-pressure-induced gels; these had a more compact matrix which expanded as storage progressed. Received: 26 May 1997     

不同气调包装对菠菜冷藏保鲜效果的影响

冷藏条件下,为研究菠菜气调包装(MAP)的最佳气体比例,延长菠菜保质期,选用农大菠杂1号为试材,以高阻隔性聚丙烯/聚乙烯(BOPP/PE)复合膜为包装袋,测定5种不同气体比例下菠菜的失重率、维生素C含量、叶绿素含量、相对电导率等理化指标的变化,并对其进行感官评价。结果表明,初始气体O₂、CO₂、N₂的体积分数分别为10%,2%, 88%时,贮藏第18 d,菠菜的感官评价5.5分,高于其他组;失重率较低,为0.32%,电导率上升较慢,为10.25%。在贮藏期最长的18 d,MA4组菠菜维生素C含量为1.25 mg/100 g,叶绿素含量为445.70 mg/kg,营养成分含量仍然较高。结论:菠菜在低温贮藏时,因呼吸作用较强,适宜的贮藏条件为高浓度的氧气(5%~10%);低浓度的二氧化碳(2%~4%)。     

Growth of the psychrotrophic pathogens Aeromonas hydrophila, Listeria monocytogenes and Yersinia enterocolitica on smoked blue cod (Parapercis colias) packed under vacuum or carbon dioxide

Commercially prepared smoked blue cod ( Parapercis colias ) was divided into 25-g subsamples which either served as uninoculated controls or were inoculated with a two-strain cocktail of one of the psychrotrophic pathogens Aeromonas hydrophila, Listeria monocytogenes or Yersinia enterocolitica. The inoculated and control subsamples were then either vacuum or carbon dioxide packed prior to storage at 3°C or −1.5°C. After various periods of storage, triplicate samples from both packaging treatments for the three pathogens and the corresponding uninoculated controls were removed and subjected to microbiological analysis. None of the three pathogens was found as a natural contaminant of the commercial product. In vacuum-packs all three psychrotrophic pathogens were able to grow during storage at 3°C. Reduction of the storage temperature to − 1.5°C retarded but did not prevent pathogen proliferation. Under carbon dioxide, only A. hydrophila was able to grow at 3°C and then only after a 21-day lag period. None of the psychrotrophic pathogens was able to grow under carbon dioxide at − 1.5°C. With the possible exception of A. hydrophila , pathogen numbers did not decline in carbon dioxide packs during 155 days storage at − 1.5°C. It is concluded that provided gross contamination with psychrotrophic pathogens prior to packaging does not occur, a 100% carbon dioxide controlled atmosphere can be used to extend the product life of smoked blue cod during storage at or below 0°C without compromising its safety in respect to growth of A. hydrophila, L monocytogenes or Y. enterocolitica.     

Inhibition of lipid damage in refrigerated salmon (Oncorhynchus kisutch) by a combined treatment of CO2 packaging and high-pressure processing

The effect of a previous combined treatment (CO₂-enriched modified atmosphere packaging, MAP and high-pressure processing, HPP, 150 MPa/5 min) on lipid stability of refrigerated (10 days/4 °C) salmon (Oncorhynchus kisutch) was studied. The following processing conditions were compared: B-0 (fish without MAP or HPP), B-1 (fish packaged under MAP and without HPP), B-2 (fish subjected to HPP without MAP) and B-3 (fish subjected to MAP and HPP). An inhibitory effect (P < 0.05) on lipid hydrolysis and oxidation was obtained by the presence of CO₂ in the packaging medium; values detected at day 10 for B-0 and B-1 fish were 80.72 and 49.61 (g free fatty acids, FFA, kg⁻¹ lipids), 6.14 and 2.81 (meq active oxygen kg⁻¹ lipids; peroxide value), 5.05 and 3.10 (mg malondialdehyde kg⁻¹ muscle), and 5.56 and 2.70 (fluorescence ratio), respectively. Furthermore, inhibition of lipid damage was observed for HPP alone; values detected at day 10 for B-2 fish were 76.24 (g FFA kg⁻¹ lipids) and 5.28 (meq active oxygen kg⁻¹ lipids). The lowest average values for lipid hydrolysis and oxidation were obtained in samples corresponding to the combined treatment (B-3 batch), differences being significant (P < 0.05) at day 10 for FFA (41.43 g kg⁻¹ lipids), peroxide (1.84 meq kg⁻¹ lipids) and fluorescence (2.50) values.     

Modeling Microbial Spoilage and Quality of Gilthead Seabream Fillets: Combined Effect of Osmotic Pretreatment,Modified Atmosphere Packaging,and Nisin on Shelf Life

ABSTRACT: The objective of the study was the kinetic modeling of the effect of storage temperature on the quality and shelf life of chilled fish, modified atmosphere-packed (MAP), and osmotically pretreated with the addition of nisin as antimicrobial agent. Fresh gilthead seabream (Sparus aurata) fillets were osmotically treated with 50% high dextrose equivalent maltodextrin (DE 47) plus 5% NaCl. Water loss, solid gain, salt content, and water activity were monitored throughout treatment and treatment conditions were selected for the shelf life study. Untreated and osmotically pretreated slices with and without nisin (2 × 10⁴ IU/100 g osmotic solution), packed in air or modified atmosphere (50% CO₂–50% air), and stored at controlled isothermal conditions (0, 5, 10, and 15 °C) were studied. Quality assessment and modeling were based on growth of several microbial indices, total volatile nitrogen, trimethylamine nitrogen, lipid oxidation (TBARS), and sensory scoring. Temperature dependence of quality loss rates was modeled by the Arrhenius equation, validated under dynamic conditions. Pretreated samples showed improved quality stability during subsequent refrigerated storage, in terms of microbial growth, chemical changes, and organoleptic degradation. Osmotic pretreatment with the addition of nisin in combination with MAP was the most effective treatment resulting in significant shelf life extension of gilthead seabream fillets (48 days compared to 10 days for the control at 0 °C).     

Quality changes during refrigerated storage of MA-packaged pre-rigor fillets of farmed Atlantic cod (Gadus morhua L.) using traditional MAP, CO2 emitter, and vacuum

ABSTRACT:  Quality changes during 3 wk of refrigerated storage (1.3 °C) were studied on pre -rigor filleted farmed Atlantic cod packed in modified atmosphere (MAP, 60% CO₂ and 40% O₂) or vacuum. The packages of MAP contained either a CO₂ emitter and low gas volume to product volume (g/p ratio) of 1.3, or a 3.9 g/p ratio and no emitter. The CO₂ level remained stable or increased in the packages with CO₂ emitter, whereas the CO₂ level in the packages with no CO₂ emitter decreased to 40% after 4 d of refrigerated storage. High levels of oxygen in the gas mixture prevented formation of trimethyl amine (TMA) during storage of the MA-packed fish, whereas the TMA content increased significantly after 10-d storage in vacuum. MA-packed samples had the highest values of 1-penten-3-ol. Sensory scores of sour, sulfur, and pungent odors were significantly higher for vacuum-packed cod compared to the 2 MA-packaging methods measured 14 d after slaughtering. No differences in sensory scores were observed between the 2 methods of MAP, and shelf life of these samples seemed to be 14 to 21 d. Cod samples packaged in vacuum packages had higher pH values compared to ordinary MAP and packages containing a CO₂ emitter. Bacterial growth was inhibited by MAP and resulted at the end of the storage period in dominance of Carnobacterium and some Photobacterium . In MA packages with high O₂ levels the Photobacterium was inhibited. It is concluded that CO₂ emitters are well suited for reduction of transport volume for MA-packaged farmed cod.     

CO2冷海水预处理结合气调包装对南美白对虾品质的影响

研究CO₂冷海水处理结合气调包装对南美白对虾的保鲜效果。新鲜南美白对虾经饱和CO₂冷海水处理,采用不同气调包装（QT1,100% CO₂;QT2,40% CO₂+60% N₂;QT3,85% CO₂+5% O₂+10% N₂;QT4,40% CO₂+30% O₂+30% N₂）后于4℃冷藏,以普通空气包装（CK1）和真空包装（CK2）为对照,比较了贮藏过程中菌落总数、pH、挥发性盐基氮、多酚氧化酶活性、质构等指标的变化。结果表明,随贮藏时间的延长,菌落总数、pH、挥发性盐基氮均呈上升趋势,且显示较好的正相关性,多酚氧化酶活性明显下降,剪切力逐渐下降。QT1、QT2和QT3均可抑制贮藏期间菌落总数增加,减缓TVB-N上升速度,同时QT2和QT3可明显抑制多酚氧化酶活性,显示出良好的防褐变效果,在CO₂冷海水预处理的基础上进一步延长2~3 d的货架期。