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为研究60Co-γ射线对榆黄菇菌丝遗传诱变的影响,采用不同的辐照剂量和不同的辐照剂量率对出发菌株榆黄菇818 的尖端菌丝进行辐照处理。结果表明:在辐照剂量为600Gy、辐照剂量率为43.5Gy/h 的条件下,菌丝再生后进行挑选,获得了生长优良的诱变菌株。将诱变菌株与出发菌株进行生长对比实验,诱变菌株菌丝生长度平均提高21.1%;经拮抗实验、酯酶和过氧化物酶同工酶实验,证明诱变菌株遗传物质发生了变化,已不同于出发菌株,具有种的特异性;经连续转接6 代进行固体培养,诱变菌株优良的生长性能得到了保持,具有遗传稳定性。 相似文献
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以榆黄菇为研究对象,对榆黄菇多糖(PCP)提取工艺进行优化、纯化、测定单糖组成和分子量,再对其体外免疫活性进行评价。在单因素基础上通过响应面法确定榆黄菇多糖最优提取工艺为:提取温度59.81 ℃,提取时间2.40 h,液料比29.91 mL/g,在此条件下,预测得率为18.82%,实际得率为18.60%。经DEAE-Sepharose Fast Flow柱层析从榆黄菇多糖中分离纯化出3个多糖组分(PCP-1、PCP-2、PCP-3),采用离子色谱法和高效凝胶渗透色谱法分析多糖成分和分子量,得出PCP-1由半乳糖组成,分子量为 1.90×104 u;PCP-2由半乳糖和葡萄糖组成,摩尔比为4.36:5.64,分子量2.76×104 u;PCP-3由岩藻糖、盐酸氨基葡萄糖、半乳糖、葡萄糖和葡萄糖醛酸组成,其摩尔比为0.07:0.11:0.51:7.46:1.85,分子量为4.81×104 u。通过体外试验评估PCP对巨噬细胞RAW264.7免疫调节活性。结果表明,PCP-1、PCP-2和PCP-3多糖质量浓度在25~200 g/mL范围内对巨噬细胞RAW264.7无毒性并具有一定增殖作用、显著提高了NO释放量并增强了巨噬细胞RAW264.7的吞噬能力。当PCP-1、PCP-2和PCP-3多糖质量浓度为200 g/mL时,巨噬细胞RAW264.7 NO释放量达到最大值,分别为11.40、11.56、11.76 moL/L。该研究结果可为榆黄菇的精深加工综合利用提供理论依据。 相似文献
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探讨不同浓度的榆黄蘑饮料的降脂作用(165、330、660g.L-1,0.1mL.10g-1BW-1)。结果显示榆黄蘑饮料能够降低蛋黄乳液所致小鼠单纯性高胆固醇症中血清中TC含量(P<0.01),榆黄蘑饮料能够下调高脂饲料所致小鼠血清TC、TG和LDL-C的含量的升高(P<0.05或0.01),并增加其血清中HDL-C的含量(P<0.05或0.01),增加小鼠食糜性高脂血症中血清SOD的活性(P<0.05),并降低MDA的含量(P<0.05或0.01)。结果表明榆黄蘑饮料对小鼠实验性高脂血症有较好的防治作用,推测榆黄蘑饮料的降脂作用是部分地通过抗氧化途径起作用的。 相似文献
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New protein PCiP from edible golden oyster mushroom (Pleurotus citrinopileatus) activating murine macrophages and splenocytes 总被引:1,自引:0,他引:1
Fuu Sheu Po‐Jung Chien His‐Kai Wang Hui‐Hsin Chang Yuan‐Tay Shyu 《Journal of the science of food and agriculture》2007,87(8):1550-1558
A new immunomodulatory protein (PCiP) was purified from an edible golden oyster mushroom (Pleurotus citrinopileatus) by extraction with 5% (v/v) cold acetic acid in the presence of 0.1% (v/v) 2‐mercaptoethanol, followed by ammonium sulfate fractionation, DE‐52 and MonoQ anion‐exchange chromatography. Electrophoresis assays demonstrated that the molecular mass of PCiP was approximately 15.0 kDa and its pI was around 5.2. PCiP is a simple protein without carbohydrate, and cannot agglutinate mouse red blood cells, suggesting PCiP is not a lectin. In addition, PCiP (5–20 µg mL?1) alone activated murine splenocytes, and markedly increased their proliferation and gamma‐interferon (IFN‐γ secretion, but suppressed MTT metabolization, while murine splenocytes were simultaneously stimulated by the mitogen concanavalin A (ConA). Furthermore, PCiP (5–20 µg mL?1) directly activated murine macrophages and increased the production of both the nitric oxide (NO) and tumor necrosis factor‐alpha (TNF‐α by RAW 264.7 macrophages. These findings suggest that PCiP could strengthen both the innate and adaptive responses of its host. Copyright © 2007 Society of Chemical Industry 相似文献
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Molecular Weight Affected Antioxidant,Hypoglycemic and Hypotensive Activities of Cold Water Extract from Pleurotus citrinopileatus 下载免费PDF全文
Pao‐Huei Chen Yih‐Ming Weng Shu‐Mei Lin Zer‐Ran Yu Be‐Jen Wang 《Journal of food science》2017,82(10):2456-2461
Cold water extract of P. citrinopileatus (CWEPC) was fractioned into 4 fractions, PC‐I (<1 kDa), PC‐II (1‐3.5 kDa), PC‐III (3.5‐10 kDa), and PC‐IV (>10 kDa), by ultrafiltration. The antioxidant activities, the inhibition of pancreatic α‐amylase, intestinal α‐glucosidase, and hypertension‐linked angiotensin converting enzyme (ACE), as well as the contents of polysaccharides, protein, and phenolic compounds of 4 fractions were determined. The results showed that lower MW fractions exerted a higher antioxidant activity, which was correlated to phenolic contents. The high molecular fraction (PC‐IV) exhibited significantly higher inhibitory activity on α‐amylase, α‐glucosidase, and ACE compared to CWEPC and the other 3 lower MW fractions (<10 kDa), which was more related to protein contents. The inhibition capability of CWEPC and PC‐IV on α‐amylase activity was 1/13.4 to 1/2.7 relative to that of acarbose, respectively. Kinetic data revealed that PC‐IV fraction followed a noncompetitive inhibition pattern on α‐glucosidase activity. The study demonstrated that various MW fractions and types of components contribute to different biological functions of P. citrinopileatus and it is protein constituents but not peptides responsible for the hypoglycemic potential of CWEPC. 相似文献
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目的:研究金顶侧耳多糖(PCP)对小鼠肝、心、肾抗氧化酶活性及同工酶表达的影响。方法:小鼠分为对照组、模型组、药物组(联苯双脂滴丸)和PCP低(100mg/(kg.d))、中(200mg/(kg.d))、高(400mg/(kg.d))剂量组,连续喂养30d后,灌胃给予CCl4(80mg/(kg.d))建立动物急性肝损伤模型。小鼠处死后分别取血液、肝、心和肾测定转氨酶及抗氧化酶活性。同时,采用聚丙烯酰胺浓度梯度凝胶电泳分析过氧化氢酶(CAT)和超氧化物歧化酶(SOD)同工酶。结果:模型组血清谷丙转氨酶(ALT)、谷草转氨酶(AST)水平显著升高,各脏器抗氧化酶活性显著降低,与对照组比差异有显著性(P<0.05或P<0.01)。给予PCP后,ALT和AST水平均显著降低,中、高剂量组能显著提高肝、心和肾CAT、谷胱甘肽过氧化物酶(GSH-Px)及MnSOD活性并能恢复到正常组水平。同工酶电泳结果表明,各脏器CAT同工酶都为1条,在造模后酶带染色密度明显降低,在给予PCP后同工酶染色密度提高并恢复到正常组水平。肝和肾有1条MnSOD带和3条CuZnSOD带,造模后酶的染色密度降低,给予PCP后能增加肝和肾总SOD和... 相似文献
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Ana C. Freitas Mariana B. Antunes Dina Rodrigues Sérgio Sousa Manuela Amorim Maria F. Barroso Ana Carvalho Sandra M. Ferrador Ana M. Gomes 《International Journal of Food Science & Technology》2018,53(8):1914-1924
Incorporating spent coffee grounds (SCGs), a by‐product from coffee brewing, in growth substrate of beneficial edible mushrooms is an approach that has to be further studied due to its potential positive outcomes: environmental impact mitigation, production costs reduction and beneficial impact on consumer health. Hence, cultivation of Pleurotus citrinopileatus and Pleurotus salmoneo‐stramineus was tested using SCG which enabled maximum production yield of P. citrinopileatus which was of 25.1% (w/w). Variable antidiabetic potential was observed between aqueous and enzymatic extracts (3.8%–29% inhibition) regardless species and substrates, whereas aqueous extract of P. citrinopileatus grown in substrate without SCG stood out presenting the highest antioxidant activity and inhibition activity of angiotensin I‐converting enzyme (IC50 = 123 μg mL?1). Ethanolic and aqueous extracts of both Pleurotus species grown in the presence or absence of SGC proved to be an interesting prebiotic source for growth of Bifidobacterium animalis Bo in comparison with fructooligosaccharides (FOS). 相似文献
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以杏鲍菇为原料,通过70%乙醇提取可溶性物质,通过体外抗氧化实验研究其乙醇提取物的抗氧化活性,通过高效液相色谱质谱联用技术(LC-MS)分析乙醇提取物的一些生物活性成分。对杏鲍菇乙醇提取物的成分分析表明,杏鲍菇乙醇提取物中蛋白质含量为2.41 mg/g(DW),总糖含量为25.46 mg/g(DW),多酚含量为12.03 mg/g(DW),黄酮含量为1.53 mg/g(DW)。体外抗氧化实验表明,杏鲍菇乙醇提取物对1,1-二苯基-2-三硝基苯肼(DPPH·)、羟自由基(·OH)、超氧阴离子自由基(O2-)都具有一定的清除能力。结果表明随着杏鲍菇乙醇提取物的浓度增大,抗氧化活性也增加。杏鲍菇乙醇提取物清除自由基能力的大小顺序为:·OH>DPPH·>O2-,而且杏鲍菇乙醇提取物对脂质过氧化具有明显的抑制作用。利用HPLC对多酚类物质进行了分析,其中含有原儿茶酸和黄岑素,利用LC-MS进一步对多酚类物质进行分析,证实其中含有:奎宁酸、没食子酸、原儿茶素、香豆酸和肉桂酸。 相似文献
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侧耳属真菌多糖的研究进展 总被引:4,自引:0,他引:4
侧耳属真菌(Pleurotus)是高等担子菌类的一种,富含独特生理活性的多糖。现代医学和药理学的很多研究表明,侧耳属真菌多糖是一类重要的“生物反应调节剂(BRMs)”,对肿瘤也有显著的抑制作用。主要阐述了几种常见侧耳属真菌多糖的制备与纯化、结构分析、生物活性以及研究展望等方面的内容。 相似文献
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D. Feretti I. Zerbini C. Zani E. Ceretti M. Moretti S. Monarca 《Food Additives & Contaminants》2007,24(6):561-572
The Allium cepa assay is an efficient test for chemical screening and in situ monitoring for genotoxicity of environmental contaminants. The test has been used widely to study genotoxicity of many pesticides revealing that these compounds can induce chromosomal aberrations in root meristems of A. cepa. Pesticide residues can be present in fruit and vegetables and represent a risk for human health. The mutagenic and carcinogenic action of herbicides, insecticides and fungicides on experimental animals is well known. Several studies have shown that chronic exposure to low levels of pesticides can cause birth defects and that prenatal exposure is associated with carcinogenicity. This study evaluated the potential application of plant genotoxicity tests for monitoring mutagens in edible vegetables. The presence of pesticides and genotoxic compounds extracted from 21 treated vegetables and eight types of grapes sampled from several markets in Campania, a region in Southern Italy, was monitored concurrently. The extracts were analysed for pesticides by gas chromatography and high-performance liquid chromatography, and for genotoxicity using two plant tests: the micronucleus test and the chromosomal aberration test in A. cepa roots. Thirty-three pesticides were detected, some of which are not approved. Genotoxicity was found in some of the vegetables and grapes tested. Allium cepa tests proved to be sensitive in monitoring genotoxicity in food extracts. The micronucleus test in interphase cells gave a much higher mutagenicity than the chromosomal aberration test in anaphase-telophase cells. 相似文献
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本文以阿魏菇中分离纯化得到的多糖-阿魏菇多糖(Pleurotus ferulae Lenzi polysaccharide,PFLP)作为研究对象,通过高效凝胶过滤色谱来测定其分子量以及分布,通过气相色谱法(GC)、部分酸水解、高碘酸氧化法、Smith降解法、甲基化反应、红外光谱(IR)、核磁共振氢谱(1HNMR)、刚果红实验和碘-碘化钾实验来分析PFLP的结构。经分离纯化后得到两种纯PFLP组分(PFLP1、PFLP2),结果分析表明,PFLP1的分子量范围为9400~9900 u;含有鼠李糖、甘露糖、葡萄糖和半乳糖四种单糖,摩尔比为1:1.54:18.6:3.64;PFLP2的分子量范围为9700~10400 u;含有鼠李糖、木糖、甘露糖、葡萄糖和半乳糖五种单糖,摩尔比为1:1.08:0.65:6.76:4.28。PFLP1和PFLP2除了都具有1→2、1→3、1→6糖苷键,还具有1→4糖苷键;都具有典型的多糖红外吸收,是以β-吡喃糖苷为主并且含有蛋白质的糖缀合物的多糖;并且都具有三股螺旋结构,有较长的侧链和分支。 相似文献
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杏鲍菇多糖对老龄小鼠抗疲劳能力的影响 总被引:4,自引:0,他引:4
目的:研究杏鲍菇多糖对老龄小鼠游泳耐力和相关生理指标的影响。方法:将小鼠随机分为对照组(A)、衰老模型组(B)、衰老用糖组(C)。 B、C 组腹腔注射D- 半乳糖(100mg/(kg bw·d)),连续60d,建立衰老模型,A 组腹腔注射生理盐水,剂量时程相同;第41 天起C 组灌胃100mg/(kg bw·d)杏鲍菇多糖,A、B 组灌胃等剂量蒸馏水,连续20d。第61 天测定小鼠的力竭游泳时间、血尿素氮、血乳酸值、血红蛋白含量及脏器指数。结果:杏鲍菇多糖能显著降低运动后血尿素氮和血乳酸含量,延长力竭游泳时间,并使血红蛋白含量及胸腺、脾脏指数显著提高(P < 0.05)。结论:杏鲍菇多糖具有抗疲劳、提高老龄小鼠运动能力的功效。 相似文献
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