共查询到19条相似文献,搜索用时 203 毫秒
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为筛选水冬瓜叶抗炎镇痛活性成分,采用高效液相色谱(HPLC)建立不同采收期10批次水冬瓜叶药材指纹图谱,对其进行主成分分析,并基于皮尔逊相关分析法探讨不同采收期水冬瓜叶药材抗炎镇痛的谱效关系。结果显示,不同采收期水冬瓜叶药材HPLC指纹图谱中有17个共有峰,10批次药材的相似度为0.875~0.973。聚类分析将不同采收期水冬瓜叶药材分为3类。主成分分析共得到3个主成分。经皮尔逊相关分析,峰17、峰14、峰12、峰15、峰13、峰5、峰16和峰4可能为水冬瓜叶药材镇痛作用的物质基础,峰6、峰8(异槲皮苷)、峰11、峰7(金丝桃苷)、峰5、峰3、峰4、峰13、峰9和峰1可能为水冬瓜叶药材抗炎作用的物质基础。 相似文献
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目的:建立不同产地威灵仙的HPLC指纹图谱,并结合化学模式识别方法对其进行分析与评价。方法:采用超声水提取,进行HPLC分析,以4个产地的13批威灵仙药材为样本,建立其HPLC指纹图谱。采用《中药色谱指纹图谱相似度评价系统》、聚类分析、主成分分析和正交偏最小二乘法分析等化学计量学方法对其进行评价。结果:所建立的13批威灵仙HPLC指纹图谱共标定4个共有峰,相似度分析结果显示,13批威灵仙药材特征性一致;聚类分析和主成分分析结果显示,13批威灵仙药材被分为两类,一类是东北铁线莲,另一类是威灵仙。结论:本研究建立的威灵仙HPLC指纹图谱能快速、科学、准确地应用到威灵仙的质量评价。 相似文献
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目的:通过建立凉粉草药材的高效液相(HPLC)指纹图谱,为科学区分及评价不同来源的凉粉草药材提供参考。方法:采用高效液相色谱仪对凉粉草提取溶液进行梯度洗脱,对比不同来源的凉粉草溶液所得色谱图,选取其中9个特征峰,对其峰面积数据采用相似度分析、主成分分析等进行数据处理。结果:建立凉粉草的指纹图谱,20批次的凉粉草相似度较高,基本大于0.9,统计学分析结果基本一致。结论:来自广东平远上举镇、泗水镇、仁居镇等地的凉粉草质量较好;指纹图谱能较好的区分不同产地的凉粉草,为其质量控制提供科学依据。 相似文献
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目的:建立不同种质地黄高效液相色谱(HPLC)指纹图谱,并测定其中6种差异性成分的含量。方法:采用HPLC法建立7个种质地黄的指纹图谱并进行相似度评价,通过主成分分析、聚类分析、偏最小二乘法-判别分析对指纹图谱进行化学模式识别分析。对不同种质地黄中的6种差异性成分梓醇、地黄苷D、地黄苷A、益母草苷、毛蕊花糖苷、异类叶升麻苷进行含量测定。结果:指纹图谱研究中地黄标定了15个共有峰,并指认出10个共有峰。聚类分析显示,BJ-1、BX与其他种质地黄存在显著差异。含量测定结果显示,各种质地黄梓醇含量较高,BJ-1地黄毛蕊花糖苷含量显著高于其他种质。结论:该研究建立的指纹图谱及含量测定方法能够快速、科学、准确地评价不同种质地黄的质量。 相似文献
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建立连翘药材的HPLC指纹图谱,为连翘药材质量的评价提供实验依据。采集不同产地的连翘药材,对生晒品、蒸品、煮品三种产地加工处理的三批连翘药材进行HPLC检测,建立以连翘苷为内标参照峰的连翘药材HPLC指纹图谱。按照建立的HPLC色谱条件,对6批市售药材进行检测,验证所得HPLC指纹图谱的实用性。三种产地加工品的HPLC图谱相似,建立了以连翘苷为内标参照峰的连翘药材HPLC指纹图谱。总共标定了20个共有峰,且共有峰面积均占总峰面积的90%以上。结果显示,6批市售药材与已知HPLC对照指纹图谱的相似度均0.90。本研究确定的连翘HPLC指纹图谱对连翘药材的鉴别具有很好的适用性、推广性。 相似文献
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《广州化工》2017,(22)
采用Agilent ZORBAX SB-C18色谱柱(4.6 mm×250 mm,5μm),乙腈(A)-水(0.2%磷酸,B)为流动相,梯度洗脱(0~15 min,5%~15%A;15~85 min,15%~35%A),流速1 m L·min-1,检测波长270 nm,柱温25℃,进样量10μL,建立了不同产地半枝莲药材的指纹图谱。其精密度、重现性以及稳定性良好。以野黄芩苷作为对照峰,标定了13个特征峰为其共有峰。采用中药色谱指纹图谱相似度软件进行测评,8份药材的相似度介于0.953~0.768。主成分分析获得2个主要成分,累积贡献率可达80.347%。应用指纹图谱并结合主成分分析,能够准确的评价半枝莲药材的质量。 相似文献
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目的:构建九味熄风颗粒的HPLC指纹图谱,获得良好的各色谱分离峰形,获悉其成分归属与含量,准确客观评估九味熄风颗粒的整体质量。方法:采用高效液相色谱法,以Kromasil C18柱(250 mm×4.6 mm,5μm)为色谱柱,以乙腈-0.1%磷酸为流动相,梯度洗脱,检测波长225 nm,流速0.5 mL/min,柱温30℃。运用相关软件,以11批次的九味熄风颗粒为研究对象,计算其化学成分指纹图谱的相似度,指认图谱共有峰药材来源,结合飞行时间质谱法检测图谱中的主要化学成分。结果:建立了九味熄风颗粒的HPLC指纹图谱;发现并确定17个具有指纹意义的共有峰,可归属到6味药材,其中6个共有峰得到鉴定;各批次九味熄风颗粒样品指纹图谱与标准指纹图谱相似度均大于0.9。结论:该法准确快速获悉各批次原药材、中间体的成分变化,为合理调配提供试验数据支撑,最大程度保证成品的成分含量及组成比例的一致性。 相似文献
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铁箍散是贵州民间治疗风湿麻木、跌打损伤的常用药材,主要有效成分为挥发油。采用水蒸气蒸馏法提取铁箍散根茎部位的挥发油,气相色谱-质谱联用技术对相对质量分数大于0.02%的挥发油成分进行了鉴定,共鉴定出54个成分,相对质量分数之和为94.02%,其中相对质量分数较高的有4-萜烯醇(20.22%)、γ-杜松烯(12.33%)、1,2,3,4,4a,7-六氢-1,6-二甲基-4-(1-甲基乙基)-萘(13.25%)、T-依兰油醇(11.85%)。该文报道工作的新颖性已为遵义医学院图书馆信息部2009年9月14日出具的第2009K043号《科技查新报告》所证实。 相似文献
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采用高压均质均质机进行了富含苯丙烯酸类化合物的天然植物的提取,分别测定了这些天然植物的95%乙醇、石油醚、水提取物对酪氨酸酶的抑制率。结果表明:(1)三种提取剂得到的提取物均对酪氨酸酶具有抑制作用的中药有川芎、当归、酸枣仁、荷莲豆、人参叶、肉桂、白附子、玄参。(2)三种提取剂得到的提取物均对酪氨酸酶具有激活作用的中药有小蓟、向日葵、茵陈、丹参。(3)兴安升麻的水提取物对酪氨酸酶表现出激活作用,而其95%乙醇和石油醚提取物则表现出抑制作用;蒲公英和金银花的水和乙醇提取物对酪氨酸酶表现出激活作用,而其石油醚提取物则为抑制作用。 相似文献
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采用火焰原子吸收分光光度法测定了张家界不同产地杜仲叶及皮中Fe、zn、Cu、Mn、Mg、K、Ca等7种矿质元素的含量,结果表明杜仲叶及皮中各种矿质元素含量丰富,各元素含量的高低趋势在杜仲叶中大致为:Mg〉K〉Ca〉Zn〉Fe〉Mn≥Cu,在杜仲皮中大致为:Mg〉Ca〉K〉Fe〉Zn〉Mn≥Cu。K—S检验可以看出杜仲叶及皮中各矿质元素含量均服从正态分布,LSD检验可以得出张家界不同产地杜仲叶中Fe、Mn、Mg及杜仲皮中Fe、Mn、K元素的含量存在差异性显著。这一结果可以为不同产地杜仲叶/皮的药理活性及其品质的差异提供一定的理论依据。 相似文献
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目的:建立柳叶蜡梅Chimonathus SalicifoliusS.Y.Hu叶挥发油傅立叶变换红外(FT—IR)指纹图谱。方法:采用水蒸汽蒸馏法提取不同产地的柳叶蜡梅叶中挥发油,采用FT-IR技术测定其红外光谱,并建立指纹图谱。结果:建立了十批柳叶蜡梅叶药材的FT-IR指纹图谱。结论:该方法所建立的挥发油FT-IR指纹图谱能为该药材的质量分析、评价与控制提供依据。 相似文献
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This article deals with looking for the best passive samplers among eleven plants diffused in the Mediterranean basin for the determination of polycyclic aromatic hydrocarbons in the atmosphere in substitution to the particulate matter sampling. The most recent research about biological passive samplers showed a quali-quantitative difference in pollutant sampling by different plants. Evergreen trees with long-lived leaves were selected in the urban area of Catania (Italy) and both samples (leaves and particulate matter) were extracted and purified by a two stage method before the analysis was performed by a GC/MS. Leaves and particulate matter were sampled in three different seasons (winter, spring and summer). PAHs concentrations found in the particulate matter and leaves were correlated. Some plants ( Olea europaea and Quercus ilex ) showed high values (r 2 > 0.85) of linear correlation coefficient in all seasons and are characterized by profiles similar to that of the particulate matter. Further, it has been observed that during the warmer seasons the total quantity of PAH adsorbed by the plants is greater but the fitting is better during winter. No difference was found by washing leaves with distilled water: this means that PAHs aren't deposited on the surface but are adsorbed in the lipidic layer of the leaves. 相似文献
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de Oliveira AM Conserva LM de Souza Ferro JN de Almeida Brito F Lyra Lemos RP Barreto E 《International journal of molecular sciences》2012,13(2):1598-1611
Sabicea species are used in the Amazon for treatment of fever and malaria, which suggests that its chemical constituents may have some effect on pain and inflammation. Phytochemical analysis of the hexane fraction obtained from the crude ethanol extract from Sabicea grisea var. grisea Cham. & Schltdl (Rubiaceae), an endemic plant in Brazil, resulted in the isolation of octacosanol. This study investigated the antinociceptive and anti-inflammatory effects of the octacosanol in different experimental models. The crude ethanolic extract and hexane fraction obtained from the leaves of S. grisea produced an inhibition of acetic acid-induced pain. Moreover, octacosanol isolated from the hexane fraction produced a significant inhibition of pain response elicited by acetic acid. Pre-treatment with yohimbine, an alpha 2-adrenergic receptor antagonist, notably reversed the antinociceptive activity induced by octacosanol in the abdominal constriction test. Furthermore, mice treated with octacosanol did not exhibit any behavioral alteration during the hot plate and rota-rod tests, indicating non-participation of the supraspinal components in the modulation of pain by octacosanol with no motor abnormality. In the formalin test, octacosanol did not inhibit the licking time in first phase (neurogenic pain), but significantly inhibited the licking time in second phase (inflammatory pain) of mice. The anti-inflammatory effect of octacosanol was evaluated using carrageenan-induced pleurisy. The octacosanol significantly reduced the total leukocyte count and neutrophils influx, as well as TNF-α levels in the carrageenan-induced pleurisy. This study revealed that the mechanism responsible for the antinociceptive and anti-inflammatory effects of the octacosanol appears to be partly associated with an inhibition of alpha 2-adrenergic transmission and an inhibition of pathways dependent on pro-inflammatory cytokines. Finally, these results demonstrated that the octacosanol from the leaves of S. grisea possesses antinociceptive and anti-inflammatory activities, which could be of relevance for the pharmacological control of pain and inflammatory processes. 相似文献
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酸模叶蓼和绵毛酸模叶蓼中脂肪酸的研究 总被引:1,自引:0,他引:1
用索氏提取法提取脂溶性成分,GC-MS联用法结合计算机检索,首次对酸模叶蓼及其变种绵毛酸模叶蓼的脂肪酸成分进行了分离和鉴定。从酸模叶蓼和绵毛酸模叶蓼中各分离鉴定出21个和13个化合物,分别占峰面积的63.26%和74.73%,其中,脂肪酸成分占53.53%和35.58%,甾类成分占9.73%和39.15%。用峰面积归一化法测定了各成分的相对质量分数,酸模叶蓼中主要成分为:花生酸(11.82%),亚麻酸(10.36%),棕榈酸(9.79%),亚油酸(8.51%),22,23-二氢豆甾醇(5.88%),γ-谷甾醇(3.85%),山嵛酸(3.15%),木蜡酸(2.6%)。绵毛酸模叶蓼主要成分为:γ-谷甾醇(20.61%),22,23-二氢豆甾醇(18.54%),亚油酸(10.41%),棕榈酸(7.42%),油酸(7.50%),花生酸(3.21%),硬脂酸(2.38%)。该文报道工作的新颖性,已为河南大学图书馆2008年9月9日出具的第CX2008008号《科技查新报告》所证实。 相似文献
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采用索氏提取法以正己烷为溶剂从樟子松和黑皮油松新鲜针叶中提取松针提取物,对提取物进行了气相色谱-质谱(GC-MS)分析,并分别研究了这2种松针提取物与松木屑木醋液的复配剂对榆紫叶甲的杀虫活性。研究结果表明:樟子松鲜针叶正己烷提取物中共鉴定出16种成分,总GC含量为65.92%,GC含量最高的化合物是2,2,4,4,6,8,8-七甲基壬烷(23.72%),其次是苯乙酸-3-己烯酯(8.56%)。黑皮油松鲜针叶正己烷提取物中共鉴定出41种成分,总GC含量为98.06%,GC含量最高的化合物是石竹烯(23.29%),其次是4-epi-荜澄茄油烯醇(11.24%)。这2种松针提取物复配木醋液(体积比1∶9)的5倍蒸馏水稀释液,对榆紫叶甲成虫的毒杀效果差,樟子松鲜针叶提取物复配木醋液(体积比1∶9)的5倍蒸馏水稀释液对榆紫叶甲成虫的毒杀效果优于木醋液的5倍蒸馏水稀释液。实验1~4天,松木屑木醋液的5倍蒸馏水稀释液对榆紫叶甲幼虫的毒杀效果要好于黑皮油松鲜针叶提取物复配木醋液(体积比为1∶9)的5倍蒸馏水稀释液,第5天则相反。 相似文献
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Dr. Letizia Giampietro Dr. Alessandra Ammazzalorso Prof. Rosa Amoroso Dr. Barbara De Filippis 《ChemMedChem》2019,14(11):1051-1066
Fibrates are a class of phenoxyisobutyric acid derivatives mainly used as anti-hyperlipidemic agents. The fibrate scaffold has undergone a variety of chemical modifications, providing a wide spectrum of biological activities. Within the last few years, the majority of new synthetic fibrate derivatives have demonstrated hypolipidemic activity through peroxisome proliferator-activated receptor α (PPARα) activation. However, some compounds containing the fibrate scaffold have shown different pharmacological properties, also independent of PPARα activation, such as anti-inflammatory, analgesic, antioxidant, and antiplatelet activities. The aim of this review is to highlight the structure–activity relationships (SAR) in evaluating the significance of fibrates in the field of medicinal chemistry. 相似文献