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大肠癌细胞膜相关抗原的光镜与电镜研究 总被引:1,自引:0,他引:1
癌细胞膜上存在特有抗原即癌细胞膜相关抗原是癌细胞的特性之一。对该种抗原的研究不仅对探索癌细胞的生物学特性,而且对诊断和治疗癌症均有重要意义。我们用结肠癌培养细胞系CLl87免疫小鼠,获得了一株能分泌抗人大肠癌单克隆抗体(单抗)的杂交瘤细胞。该单抗命名为ND—1,其对应抗原命名为LEA(Large External Antigen)。对603例人体各种组织的检测证实,LEA在大肠癌细胞的表达有高度的特异性。从表达情况看,癌细胞分化程度越高,表达得越明显(在高分化癌阳性率为100%,中分化为78.6%,低分化为0)。理化分析表明,LEA是一大分子量糖蛋白抗原(MW>500kd),唾液酸是ND—1单抗识别的抗原位点中的重要成分。本文用ND—1单抗对LEA抗原分子的分布及定位特点等在光镜、电镜水平进行了研究。 相似文献
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目的为了得到较高纯度的MUC1抗体BC2。方法应用RESOURSEQ离子交换柱进行阴离子交换层析 ,对腹水中的MUC1单抗BC2进行纯化。结果所得BC2抗体纯度约为 90 %。结论所获得高纯度的BC2抗体可做为筛选配基 ,用于下一步噬菌体随机肽筛选MUC1抗原模拟表位。 相似文献
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目的:研究双氢青蒿素(Dihydroatemisinine,DHA)对人骨肉瘤细胞143B的影响及所导致的形态学变化。方法:采用人骨肉瘤143B细胞株,通过设置对照组和低浓度、中浓度、高浓度的DHA组,H.E染色观察Trandwell小室穿梭能力;划痕愈合实验观察迁移能力;Hoechst33258荧光染色检测细胞凋亡;MTT法检测细胞增殖抑制能力。结果:DHA表现出明显地抑制人骨肉瘤143B细胞的迁移、增殖以及促进凋亡(P〈0.05),且伴随浓度的递增和时间的延长,抑制作用更明显。结论:DHA具有较强的抗入骨肉瘤作用,其机制可能与DHA诱导骨肉瘤细胞凋亡,并抑制其增殖有关。 相似文献
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为了实现工业洗衣机智能化、网络化功能,以μC/OS-Ⅱ嵌入式操作系统为开发平台,设计了控制系统的软件。将系统任务划分为显示界面、命令输入、洗涤流程控制、电机控制、模拟量检测、数字量检测、阀控制、混浊度检测、智能推理及报警等子任务,建立了系统多任务模型,并分析了显示、洗涤控制两个典型任务的执行过程。μC/OS-Ⅱ平台上开发的工业洗衣机智能控制系统人机界面友好,有完善的智能控制、检测功能,运行稳定。 相似文献
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简述半导体芯片制造中所用气体的纯度标准,并通过实例,对制备方法及流程、输送系统设计及特点、气体站设计以及参数的检测等进行了阐述。 相似文献
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黄瓜绿斑驳花叶病毒( CGMMV)是危害西瓜生产的重要病害,造成西瓜果实腐烂,失去商业价值。应用透射电子显微镜和单抗dot?ELISA技术对采自浙江温岭的西瓜病叶进行快速病原检测,电镜负染色观察到病株汁液中存在大量刚直杆状的病毒粒子,超薄切片观察到细胞质内存在整齐排列的杆状病毒聚集体,与烟草花叶病毒属( Tobamovirus)的形态特征相符;进一步采用dot?ELISA技术进行血清学检测,显示西瓜病叶与CGMMV特异性抗体呈阳性反应,检测结果表明感染西瓜的杆状病毒为CGMMV。从而为侵染西瓜的CGMMV提供快速检测途径。 相似文献
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目的:探讨利用激光扫描共聚焦显微镜鉴定单克隆抗体特异性。方法:正常人胆管组织、正常人肝组织及人胆管癌组织切片经免疫荧光染色后置于普通荧光显微镜及激光扫描共聚焦显微镜进行观察,比较二者效果。结果:与普通荧光显微镜相比,激光 聚焦显微镜能清晰地显示染色组织的荧光染色部位及强弱,能较好地显示染色组织的形态,显示出良好的准确性及精确性。结论:激光扫描共聚焦显微镜在免疫荧光染色切片的检测中具有准确、特异、清晰的特点,在单克隆抗体的鉴定中具有良好的应用前景。 相似文献
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目的:检测分化抑制因子id1在人骨肉瘤组织中的表达情况,并分析与患者临床特点、肺转移及两年生存率的关系。方法:运用免疫组织化学法检测39例人骨肉瘤,20例骨软骨瘤组织中id1的表达情况,结合临床资料和随访结果进行统计分析。结果:骨肉瘤组织中id1的表达明显高于骨软骨瘤,差异具有统计学意义(P〈0,05),其表达水平与患者Enneking临床分级有关,与患者年龄、性别无关。id1表达阳性的患者肺转移发生率高于阴性患者,两年生存率低于阴性患者。结论:id1在人骨肉瘤组织中高度表达,并与患者肺转移,两年生存率密切相关,可能作为骨肉瘤生物治疗的新靶点。 相似文献
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Immunoelectron microscopic study of the opsin distribution in the photoreceptor cells of Drosophila melanogaster. 总被引:1,自引:0,他引:1
Distribution of opsin in the compound eye of Drosophila melanogaster was examined by post-embedding immunoelectron microscopy using a monoclonal antibody against the Drosophila Rh1 opsin and gold-conjugated secondary antibody. Numerous gold particles were observed on the rhabdomeric microvilli of R1-R6 retinular cells. In the retinular cell body, gold particles were distributed in rough endoplasmic reticulum, subrhabdomeric cisternae (SRC), multivesicular bodies, and secondary lysosomes. In the rdgA mutant, whose SRC are absent, density of gold particles on the rhabdomeric microvilli was about 20% of normal. These results suggest the involvement of SRC in opsin transport. 相似文献
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基于具有广泛应用前景的ARM Cortex-M3微处理器体系结构,将μC/OS-Ⅱ实时操作系统移植到微处理器LM3S8962。通过分析微处理器LM3S8962和μC/OS-Ⅱ内核结构,并且结合ARM Cortex-M3体系特点,利用ARMCortex-M3所固有的嵌套向量中断控制器(NVIC)和Thumb-2指令集体系结构(ISA),完成了移植μC/OS-Ⅱ所需要的函数的编写,宏的定义和任务堆栈的实现,特别是利用ARM Cortex-M3所提供的新型中断-可挂起系统调用(PendSV)作为实时系统完成任务切换的软中断。给出了部分移植函数的代码,未给出代码的函数也对函数结构进行了详细的描述。通过对移植进行测试,结果表明在时钟频率为20 Hz的情况下系统运行正常,能够正常的完成任务切换,实现了移植的目的。 相似文献
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Savinska LO Lyzogubov VV Usenko VS Ovcharenko GV Gorbenko ON Rodnin MV Vudmaska MI Pogribniy PV Kyyamova RG Panasyuk GG Nemazanyy IO Malets MS Palchevskyy SS Gout IT Filonenko VV 《Eksperimental?nai?a onkologii?a》2004,26(1):24-30
AIM: To express recombinant S6K2 in baculovirus expression system; to purify large quantities of recombinant S6K2 for biochemical studies; to generate and characterise specific MABs against recombinant S6K2; to study the patterns S6K1 and S6K2 expression and subcellular localization in normal, benign and malignant breast tissues. METHODS: Recombinant baculovirus, expressing wild type S6K2 was generated using Bac-to-Bac system (Invitrogen); recombinant S6K was purified from infected Sf9 cells using affinity purification approach; monoclonal antibodies against recombinant S6K2 were generated; the specificity of generated MABs towards recombinant and endogenous S6K2 were examined by ELISA, Western blotting, immunoprecipitation and immuhohistochemical staining; immunohistochemical detection of S6K1 and S6K2 in human breast tissues was performed using specific monoclonal antibodies towards S6K1 and S6K2. RESULTS: Large amounts of enzymatically active S6K2 were purified using baculovirus expression system; highly purified preparations of S6K2 were used to generate and characterize anti-S6K2 MABs; elevated levels of S6K1 and S6K2 were found in breast tumors when compared to normal breast tissues; S6K2 is frequently localized in the nuclei of adenocarcinoma tissues, but rarely in fibroadenoma or "normal" breast tissues. CONCLUSION: Production of recombinant S6K2 in large amount and generation of specific monoclonal antibodies towards S6K2 has provided us with excellent tools to study the function and regulation of this important signalling molecule in normal and cancer cells. Immunnohistochemical analysis of S6K1 and S6K2 expression in normal and malignant breast clearly indicates that both kinases are overexpressed in breast tumors, when compared to "normal" tissues. The retention of S6K2 in the nuclei of malignant cells may be caused by disregulation of nucleocytoplasmic shuttling and could subsequently affect cell growth and proliferation. 相似文献
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Andrew JS Anglin EJ Wu EC Chen MY Cheng L Freeman WR Sailor MJ 《Advanced functional materials》2010,20(23):4168-4174
Nanostructured mesoporous silica (SiO(2)) films are used to load and release the monoclonal antibody bevacizumab (Avastin) in vitro. A biocompatible and biodegradable form of mesoporous SiO(2) is prepared by electrochemical etching of single crystalline Si, followed by thermal oxidation in air at 800 °C. Porous SiO(2) exhibits a negative surface charge at physiological pH (7.4), allowing it to spontaneously adsorb the positively charged antibody from an aqueous phosphate buffered saline solution. This electrostatic adsorption allows bevacizumab to be concentrated by >100× (300 mg bevacziumab per gram of porous SiO(2) when loaded from a 1 mg mL(-1) solution of bevacziumab). Drug loading is monitored by optical interferometric measurements of the thin porous film. A two-component Bruggeman effective medium model is employed to calculate percent porosity and film thickness, and is further used to determine the extent of drug loading into the porous SiO(2) film. In vitro drug release profiles are characterized by an enzyme-linked immunosorbent assay (ELISA), which confirms that the antibody is released in its active, VEGF-binding form. The nanostructured delivery system described here provides a sustained release of the monoclonal antibody where approximately 98% of drug is released over a period of one month. 相似文献
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随着科技的发展,嵌入式系统的应用越来越广泛,为了进行射频功率校准系统的嵌入式软件开发,需要将嵌入式实时操作系统μC/OS-Ⅱ移植到sharp lh79520微处理器上。分析了嵌入式实时操作系统μC/OS-Ⅱ的代码结构,接着,对目前流行的嵌入式微处理器sharp lh79520的特点进行了说明,详细介绍了μC/OS-Ⅱ在sharp lh79520处理器上的移植过程,特别对OS_CPU_A.ASM文件的修改给出了详细的移植代码,最后对移植的代码进行了严格的测试,结果表明移植后的μC/OS-Ⅱ操作系统内核运行稳定可靠,验证了移植的成功。 相似文献