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蚕丝是一种天然的高分子材料,由60%~80%的丝素、15%~35%的丝胶及约5%的石蜡、色素、糖类等杂质组成。丝素具有良好的生物相容性和降解性,在药物缓释、促进伤口愈合、组织工程支架等生物医药领域及降血糖、抑菌等医疗保健方面均具有广泛应用;丝胶包裹在丝素纤维外层,对丝素起保护作用,其不仅是非常有用的生物材料,还具有许多生物活性和药理作用,如抗氧化性、抗肿瘤活性、物理胃肠功能等作用。近年,蚕丝蛋白在生物样本保存领域的应用备受研究者关注,本文对蚕丝蛋白在生物样本低温保存和常温保存方面的应用和成果、存在的问题及未来的研究方向作一综述,以期为蚕丝蛋白在生物样本保存领域的应用提供参考。 相似文献
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对聚氯乙烯树脂与家蚕丝素蛋白共混膜的吸湿、热力学和染色等性能进行了研究。混合膜的酶降解特性、水分吸收和热稳定性比丝素单一膜有了较大的改进。该膜染色的外观和天然丝素膜一样透明,但其力学性能随着家蚕丝素蛋白比率的增加而下降明显。研究表明丝素蛋白粉可作为塑料的生物崩解过程的催化剂。 相似文献
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文章研究采用琥珀酰酐对废蚕丝丝素蛋白进行化学改性,研究丝素蛋白浓度、琥珀酰酐用量、反应温度、反应时间对丝素蛋白琥珀酰化程度的影响。通过正交实验确定丝素蛋白琥珀酰化的适宜条件为:丝素蛋白浓度2%、琥珀酰酐用量15%、温度40℃、反应时间30 min。丝素蛋白经琥珀酰化修饰后,起泡特性与乳化特性与原来相比均有不同程度的改善。 相似文献
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天然丝素在化妆品中的护肤特性及应用 总被引:1,自引:0,他引:1
贾艳梅 《精细与专用化学品》1999,7(14):13-14
天然丝素是世界上最受欢迎的天然型营养化妆品添加剂之一。蚕丝经处理、加工可制成丝素粉、丝肽液、丝胶冻及丝素氨基酸等,本文详细阐述了这几种产品的加工、性能及作用机理,并提供了详细的应用配方。 相似文献
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Yasushi Tamada 《应用聚合物科学杂志》2003,87(14):2377-2382
Silk fibroin (Bombyx mori) was sulfated with an aqueous sulfuric acid solution. The sulfated fibroin was characterized by Fourier transform infrared spectroscopy (FTIR), amino acid analysis, and gel filtration chromatography (GFC). Maximum yield was obtained at around 2–4 h, and it decreased at 6 h and more. The molecular size decreased and dispersed with sulfation, and the molecular weight was estimated at around 10,000 by GFC using protein standards. The amino acid composition indicated that the crystal region of the fibroin molecule remained in sulfated fibroin until a sulfation reaction time of 4 h. The incorporation of sulfate groups was confirmed by FTIR and the amount of sulfate groups introduced for 4 h sulfation was estimated in 0.3 mmol/g by acidimetric titration. The efficiency of sulfation was calculated at 15.7%. Blood coagulation was prevented by 20 mg of sulfated fibroin in 1 mL of blood, while original fibroin did not show the effect. This result indicates that sulfate group introduction results in addition of anticoagulant function to silk fibroin. Although a variety of polymer backbones have been used for synthesis of sulfated polymers as anticoagulant materials, no reports are available concerning a sulfated polymer based on a protein backbone. Sulfated fibroin is a new type of anticoagulant material having a protein backbone. © 2003 Wiley Periodicals, Inc. J Appl Polym Sci 87: 2377–2382, 2003 相似文献
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Mechanical measurements were employed to investigate the effects of three types of superfine silk protein powder on tensile strength, elongation, and elasticity of wet‐spun Pellethane® 2363‐80AE polyurethane (PU) fiber. These superfine silk protein powders included undegummed silk (with both native silk fibroin and sericin, water insoluble), native silk fibroin (with native silk fibroin only, water insoluble), and regenerated silk fibroin (with regenerated silk fibroin only, water soluble) in powder form. Experimental data derived from the mechanical measurements illustrated that the miscibility between the PU and regenerated silk fibroin were superior to that between PU and the other two silk proteins. This may be attributed to the similar chemical structure and microphase separation of PU and regenerated silk fibroin with lower molecular weight than native silk fibroin. This preliminary work may provide some information for biomimetic processing of silk‐inspired PU biofibers, which combine elasticity of synthetic PU with biofunction of natural silk fibroin for special biomedical applications. © 2009 Wiley Periodicals, Inc. J Appl Polym Sci, 2009 相似文献
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Hiroaki Sasaki Nobuo Donkai Hiraku Ito Katustoshi Imamura Hideaki Morikawa 《Coloration Technology》2000,116(11):349-351
The dyeing properties were investigated for silk proteins produced by silkworms reared entirely on an artificial diet (A) and a mulberry leaf diet (M). The dyeing experiments were carried out with CI acid orange 7 at pH 3.9 and 60°C. The sericin contents of the A and the M raw silk samples were determined to be 26.0% and 23.7%, respectively. The equilibrium dye uptake and the dyeing rate of the A silk fibroin were almost the same as those of the M silk fibroin. The equilibrium dye uptake on each silk sericin was 3–4 times higher than that of the silk fibroin. On the other hand, the equilibrium dye uptake of the A silk sericin was higher than that of the M silk sericin, but the dyeing rate of the former was slower than that of the latter. 相似文献
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Seyed T. Mousavi Garry R. Harper Sofia Municoy Mark D. Ashton David Townsend Ghazi H. K. Alsharif Vasileios K. Oikonomou Melike Firlak Sophie Au‐Yong Bethany E. Murdock Geoffrey R. Akien Nathan R. Halcovitch Sara J. Baldock Mohamad Fazilati Oleg V. Kolosov Benjamin J. Robinson Martin F. Desimone John G. Hardy 《大分子材料与工程》2020,305(6)
Biomaterials capable of controlling the delivery of drugs have the potential to treat a variety of conditions. Herein, the preparation of electrically conductive silk fibroin film‐based drug delivery devices is described. Casting aqueous solutions of Bombyx mori silk fibroin, followed by drying and annealing to impart β‐sheets to the silk fibroin, assure that the materials are stable for further processing in water; and the silk fibroin films are rendered conductive by generating an interpenetrating network of a copolymer of pyrrole and 3‐amino‐4‐hydroxybenzenesulfonic acid in the silk fibroin matrix (characterized by a variety of techniques including circular dichroism, Fourier‐transform infrared spectroscopy, nuclear magnetic resonance, Raman spectroscopy, resistance measurements, scanning electron microscopy‐energy dispersive X‐ray spectroscopy, thermogravimetric analysis, X‐ray diffraction, and X‐ray photoelectron spectroscopy). Fibroblasts adhere on the surface of the biomaterials (viability assessed using an (3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide) assay and visualized using a confocal microscope), and a fluorescently labeled drug (Texas‐Red Gentamicin) can be loaded electrochemically and released (µg cm?2 quantities) in response to the application of an electrical stimulus. 相似文献
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室温下,将再生纤维素与透明质酸(HA)、肝素(Hep)、丝素(SF)、甲壳素(N-ACS)等的复合纤维的纺丝液溶于NaOH溶液中,通过喷丝头(孔径0.1mm)喷入含有40%~43%硫酸铵的10%硫酸溶液中,得到产率为75%~98%的新型白色、柔韧性较好的复合生物纤维。FT-IR结果显示,复合纤维组分间存在物理吸附和氢键相互作用;少量的HA、Hep、SF和N-ACS等纤维的添加可增加复合纤维的韧能,其中纤维素-SF复合纤维在SF含量为10%时纤度为9.9旦,韧度值为1.08g/旦,伸长率为35.0%,机械性能相对最好。SEM图像中可以看出复合纤维表面呈现条纹、鳞状、填充和均匀包覆的结构,复合纤维间存在一定的相容性;复合纤维直径为19~55μm,密度为0.1~0.36旦/μm。 相似文献
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Preparation of non-woven mats from all-aqueous silk fibroin solution with electrospinning method 总被引:3,自引:0,他引:3
In the present study, we successfully prepared non-woven mats from stable regenerated silk fibroin aqueous solution at high concentration. Scanning electronic microscope (SEM) was used to observe the morphology of the fibers. The structure of the fibers was characterized using Fourier transform infrared (FTIR), wide-angle X-ray diffraction (WAXD) and differential scanning calorimetry (DSC). The mechanical tests were also performed. In the as-spun fibers, silk fibroin was present in a random coil conformation, the stress and strain at break were 0.82 MPa and 0.76%, respectively, while after methanol treatment, the silk fibroin was transformed into a β-sheet-containing structure, the stress and strain at break increased to 1.49 MPa and 1.63%, respectively. This study provided an option for the electrospinning of silk fibroin without using organic solvent or blending with any other polymers, which may be important in tissue engineering scaffold preparation. 相似文献
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Andiyappan Muthumanickkam Sundaramoorthy Subramanian Mahizhappan Goweri Winfred Sofi Beaula Venkataraman Ganesh 《Iranian Polymer Journal》2013,22(3):143-154
Mulberry silk fibroin is being used as biomaterial for tissue engineering applications. In the present work, comparisons are made between mulberry and eri silk fibroin scaffolds prepared by electrospinning method. The scaffolds are treated with ethanol to improve their dimensional stability, and the physical and chemical properties of the scaffolds are assessed using thermogravimetric analyzer (TGA), differential scanning calorimetry, Fourier transform infrared spectroscopy and X-ray diffractometry. The FTIR spectra confirm the structural change of silk fibroin from α-helical to β-sheet structure when mulberry and eri silk scaffolds are treated with ethanol. The thermal stability of the eri silk scaffold is found to be better than that of mulberry silk. Ethanol-treated eri silk displays higher tensile stress than the ethanol-treated mulberry silk. The hemolysis percentages of eri silk and mulberry silk scaffolds are found to be 1 and 3 %, respectively. While the platelet adhesion on eri silk fibroin scaffold is found to be lower than that of mulberry silk fibroin scaffold, the cell attachment, binding and spreading of L6 fibroblast cells on the eri silk scaffold are better than those on the mulberry silk fibroin, and the cell viability is found to be better on eri silk fibroin scaffold. 相似文献
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丝素蛋白在离子液体中的溶解特性研究 总被引:2,自引:0,他引:2
文章以离子液体为溶剂,研究了丝素蛋白在离子液体中的溶解特性。首先研究了丝素蛋白在不同结构的离子液体1-烯丙基-3-甲基咪唑氯化物([AMIM]C1)和1-丁基-3_-甲基咪唑氯化物([BMIM]c1)中的溶解速率和溶解度。结果发现,相同条件下,丝素蛋白在[AMIM]cl中具有较快的溶解速率和较大的溶解度;随温度的升高,溶解速度加快,合适的温度为95~105℃;随着丝素蛋白浓度的增大,相同条件下丝素蛋白在离子液体中的溶解速度减慢。XRD结果表明,丝素蛋白的非晶相部分首先被溶解,晶相部分结构被破坏后与溶剂作用逐渐被溶解。采用粘度法测定了丝素蛋白分子质量随溶解时间和温度的变化,结果表明:随加热时间增加和温度升高,蛋白质分子质量减小。为保证丝素蛋白的稳定性和溶解速率,温度一般控制在100℃以下,溶解时间小于5h。 相似文献
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Acid dyes are employed for commercially dyeing silk, which results in ionic bonds between the silk fibroin and the dye. This generally leads to low wet fastness properties for dyed silk fabrics. In this work, three commercial acid dyes with aromatic primary amine structures were selected to dye silk using a Mannich‐type reaction, resulting in improved wet fastness of dyed silk by forming covalent bonds between silk fibroin and dye. The Mannich‐type reactive dyeing was applied to silk fabrics at both 30 and 90°C in trials. Dyeing at 90°C can shorten the dyeing time compared with dyeing at 30°C, even although dye exhaustion and relative fixation at 90°C were a little lower. The dyeing process was optimised when the dyeing temperature was 90°C, dyebath pH 4, dye‐to‐formaldehyde ratio 1:30 and holding dyeing time 60 minutes. The results showed that the dye exhaustion on silk fabrics for the three aromatic primary amine‐containing acid dyes exceeded 94% and their relative fixation was over 80%. Their washing and rubbing fastness reached grade 4 or higher. Hence, the colour fastness properties of dyed silk fabrics using the Mannich‐type reactive dyeing method is superior to the conventional acid dyeing method using the same aromatic primary amine‐containing acid dyes. The Mannich‐type reactive dyeing for silk fabrics at 90°C can be developed into a novel and rapid reactive dyeing method, promising an effective dyeing process with excellent colour fastness. 相似文献