Shelf Life Extension of Toasted Groundnuts through the Application of Cassava Starch and Soy Protein-Based Edible Coating

The use of cassava starch and soy protein concentrate edible coatings containing 20% glycerol in extending the shelf life of toasted groundnut during ambient (27 ± 1°C) storage for 14 days was studied. Chemical indices of oxidative rancidity and sensory parameters were evaluated using standard procedures. Moisture uptake, peroxide and thiobarbituric acid values of uncoated groundnuts were higher than 100% cassava starch coated groundnuts while toasted groundnuts coated with 50:50 (cassava starch:soy protein concentrate) had the lowest values. Toasted groundnuts coated with 50:50 (Cassava starch:soy protein concentrate) film had higher colour, taste, texture and overall acceptability scores than toasted groundnuts coated with 100% cassava starch film and control. The use of 50:50 (cassava starch:soy protein concentrate) edible coatings on toasted groundnut extended the shelf life of toasted groundnuts for 14 days compared to uncoated toasted groundnuts which developed objectionable taste after second day of storage at ambient (27 ± 1°C) condition.     

Studies on the Characteristic Properties of Fermented,Sun-Dried Orange-Fleshed Sweet Potato Flour

Microbiological, functional, chemical and sensory properties of flours of two (2) cultivars of orange-fleshed sweet potato (centinnial and CIP 440293) being multiplied for distribution in South East and South-South Nigeria were investigated using standard techniques. The flours were observed to have good functional properties and high dry matter contents which were respectively 88.09 ± 0.00% and 86.0 ± 0.00%. The respective values for fat content (0.85 ± 0.00% and 1.75 ± 0.00 %), ash content (2.04 ± 0.00% and 2.40 ± 0.42%) and crude fibre content (2.90 ± 0.28% and 2.67 ± 0.00%) were low but the respective levels of carotenoids values (3.74 ± 0.00 and 5.28 ± 0.00 μg/g) were high indicating a promising source of pro-vitamin A. The sensory evaluation had no statistical difference among the parameters. The flours had high nutritive values with good functional and sensory properties. The microbial loads were within the acceptable limit of < 10⁵ recommended by ICMSF, making the flour samples microbiologically safe and with appropriate packaging, the shelf life could be extended under good storage conditions.     

Effect of two ethnic processing technologies on reduction and composition of total and non-glucosidic cyanogens in cassava

The effectiveness of two different processing methods on reduction of total and non-glucosidic cyanogens in cassava was studied in two ethnic groups in Nkhota Kota, Malawi. Cassava cyanogen content was periodically monitored during processing. Total cyanogen reductions of 97.9 ± 0.5% and 82.4 ± 1.0% were obtained upon soaking peeled and unpeeled roots, respectively. The residual cyanogen content (2.6 ± 0.7 mg HCN eq./kg) in flour produced from peeled roots was lower than the FAO/WHO limit (10 mg HCN eq./kg). The flour from the unpeeled method contained twenty times more residual cyanogen levels (53.8 ± 1.8 mg HCN eq./kg dry matter). The peels exhibited four times higher cyanohydrin than the pulp. Inclusion of the peel during processing therefore leads to high retention of cyanogens in the pulp. Clearly, soaking of peeled roots is a more effective method and thus should be promoted among ethnic communities that soak unpeeled roots.     

Use of continuous bubble separation process for separating and recovering starch and mucilage from yam (Dioscorea pseudojaponica yamamoto)

The yam (Dioscorea pseudojaponica Yamamoto) tuber is abundant in both starch and mucilage. It is difficult to separate yam starch from its tuber because of the presence of viscous polysaccharide polymers (glycoprotein). The surface-active polysaccharide-containing complexes are capable of forming foams. The goal of this study was to develop a continuous bubble separation process for separating and recovering starch and mucilage from yam tubers in the absence of undesirable chemical additives or treatments. The results showed that the highest concentration (13.2 g/100 g) of yam slurry mucilage was obtained with a reasonably high yield of recovered starch (91.2 g/100 g) by treating the starch paste with 1 g/100 g Viscozyme^® L and Pectinex^® Ultra SP-L. In comparison with the total amount of yam fed into the system, the yield of soluble carbohydrates and protein in separated mucilage were 98.8 g/100 g and 74.1 g/100 g, respectively. The chemical composition of the separated freeze-dried mucilage was shown to be high in soluble carbohydrates (52.5 g/100 g, dry basis) and protein (40.3 g/100 g, dry basis). A very low amount of starch (0.8 g/100 g) remained in the mucilage. This method is simple, low in operational and capital costs, and especially suitable for polysaccharide–protein-containing complexes recovering from a starch-containing system.     

High pressure processing (HPP) of honey for the improvement of nutritional value

The present study was undertaken to assess the effect of high pressure processing (HPP) on total phenolic content (TPC) in manuka honey. Manuka honey is known for its amazing antimicrobial action and antioxidant properties. The effect of HPP (200, 400 and 600 MPa) at ambient and moderate temperatures (53.41 ± 0.30 °C, 65.29 ± 1.77 °C, 71.92 ± 1.63 °C) and their combination for different processing time (5, 10 and 15 min) was investigated. Conventional thermal processing (51.74 ± 0.03 °C, 61.90 ± 0.10 °C and 71.58 ± 0.04 °C) was also carried out as comparison to HPP. Operating HPP at 600 MPa (26.80 ± 0.95 °C–30.18 ± 2.14 °C) for 10 min was found to be the most effective process with 47.16% increment in TPC as compared to unprocessed honey, whereas no significant increase (p < 0.05) was observed in thermal processing as well as in combined HPP–thermal processing. Therefore, HPP at ambient temperatures could be an appropriate method to produce tastier and more nutritive manuka honey.Industrial relevanceThe preservation of total phenolic content (TPC) as a main phytochemical component in honey is very important with direct impact on nutritional value and antioxidant activity. A significant increase in the TPC was obtained by processing. Results demonstrated the HPP capability to increase TPC in manuka honey by 47%. From a nutritional perspective, this result is associated with the production of a higher antioxidant honey, known to prevent certain diseases such as cancer. The study generates a new approach in honey processing which can guarantee the high nutritional quality of honey and its original natural freshness.     

Effect of Dry Heat Pre-Treatment (Toasting) on the Cooking Time of Cowpeas (Vigna unguiculata L. Walp)

Four cowpea varieties (Brown beans, Oloka beans, IAR48 and IT89KD-288) were toasted at 105 °C, and used to study the effect of dry heat treatment on the cooking time and nutrient composition of cowpea seeds and also its effect on the functional properties of resultant flour of the cowpea seed varieties. Toasting reduced the cooking time for brown and oloka cowpea varieties from 55.00–31.00 and 70.67–51.67 min, respectively. The cooking time for IAR48 and IT89KD-288 cowpea varieties increased from 104.67 to 106.00 and 88.00 to 88.67 min, respectively. The results indicate that the cooking time of cowpea seeds can be reduced significantly on toasting, while maintaining their potential as functional agents in the food industry for nutrition and utilisation. Correlation between the amount of water imbibed by cowpea seeds and their cooking time was -0.74, but was not significant at p ≤ 0.05, suggesting that there is no significant relationship between the amount of water imbibed by cowpea seeds and their cooking time.     

Structural and physicochemical properties and in vitro digestibility of recrystallized linear α-d-(1 → 4) glucans derived from mild-acid-modified cassava starch

Molecular structure and recrystallization method influence the techno-functional behaviour of recrystallized starch as a functional ingredient in foods. The physicochemical properties of debranched and recrystallized mild-acid-modified cassava starch were studied. Cassava starch was treated with 0.14 mol/L hydrochloric acid for 24, 96 and 216 h at 40 °C prior to debranching with pullulanase. The debranched starches (DS) were recrystallized by annealing (ANN-DS), temperature-cycling (TC-DS) or heat-moisture treatment (HMT-DS) and the particle distribution, crystallinity, thermal properties, solubility, water binding and in vitro digestibility were analyzed. Acid treatment increased the fraction of linear α-d-(1 → 4) glucans comprising 13–30 monomers. Particles comprised loosely to firmly coalesced primary elements forming aggregates of mono- or bi-modal size distribution at ?5 μm and ?20 μm. The relative crystallinities ranged between 31.1–56.1%. Water binding decreased significantly with acid treatment whereas both solubility and water binding were influenced by the recrystallization method and decreased in the order: DS > ANN-DS > TC-DS > HMT-DS. Major thermal transitions occurred at 80–130 °C and 130–160 °C, and the in vitro digestibility rates of 6.8–62.8% correlated significantly (p < 0.001) with relative crystallinity and melting enthalpy.     

Effect of radio frequency (RF) heating on the texture,colour and sensory properties of a comminuted pork meat product

Radio frequency (RF) cooking is a form of dielectric heating in which products are heated by subjecting them to an alternating electromagnetic field between two parallel electrodes. Although similar in some respects to Microwave heating, RF has been proposed to be more suitable for industrial heating of meats because of the greater penetration depths possible with this technology. In this study an RF cooking protocol was developed and its effect on selected quality attributes of pork based white pudding was examined. Whilst cooking of the product in air proved unfeasible due to arcing, use of a polyethylene cell with circulating hot water (80 °C) facilitated successful heating of the product. Application of RF using an optimised cooking protocol (RF power = 450 W, cell volume = 500 ml and continuous circulation) resulted in a mean end-point temperature of 73 °C after 7 min 40 s. Similar mean end-point temperatures in water bath and steam oven heated products were achieved after 29 and 33 min, respectively. A factorial experiment was conducted to assess selected quality attributes of the cooked puddings. Results show that RF heated puddings were not significantly different (P > 0.05) from water bath and steam oven heated products with regard to instrumental colour, instrumental texture (Kramer shear and texture profile analysis) and expressible fluid. Furthermore, results of a sensory similarity test involving 60 panellists indicated that panellists were not able to detect differences between puddings cooked by RF and conventional methods. Overall this suggests that RF heating technology could have potential in pasteurisation of meat products though further work is needed to verify this.     

Rapid (microwave) heating rate effects on texture,fat/water holding,and microstructure of cooked comminuted meat batters

Comminuted and gelled, fat-containing meat products such as frankfurters and luncheon meats are commercially processed by heating relatively slowly (for up to 2 h or more) to an endpoint of about 70 °C prior to cooling. This study compared such a slow, ramp heating regime (0.5 °C/min), terminated at 70 °C, to rapid, square-wave cooking (one step: rapid 100 °C/min heating to 70 °C endpoint, plus isothermal holding prior to cooling, or two-step: rapid heating to 50 °C, holding, then rapid heating to 70 °C plus holding prior to cooling) on meat batter gel properties (fracture and small strain rheology, microstructure, cook loss, and expressible water). The results indicated that a rapid cooking process, with its inherent advantages of reduced process time, lower equipment footprint, and more efficient use of energy, can produce a product nearly equivalent in textural properties and cook yield to one processed by traditional smokehouse cooking when the cook value of the processes is similar and an intermediate (near 50 °C) holding step is included (two-step rapid heating). One-step rapid heating negatively affected gel structural homogeneity and water/fat holding properties of fat-containing gels.     

Chemical composition and potential of some underutilized tropical biomass. I: fluted pumpkin (telfairia occidentalis)

The seeds of Telfairia occidentalis have been subjected to standard chemical analysis to evaluate their properties. Proximate analysis indicated a low moisture content (6.30 ± 0.50%). The ash content was slightly higher than the range recommended for compounding of animal feed (3.44 ± 0.06%). The carbohydrate content was low (16.5 ± 0.12%). Starch, however, constituted the dominant carbohydrate (62.5 ± 0.48), while three sugars, glucose, fructose and sucrose were detected in the seed. The crude protein in the seed was high (16.0 ± 0.03%), a value which compared favourably with high protein seeds and nuts. In all, 16 amino acids were detected in the protein. Glutamic acid showed the highest concentration (16.4 g 100 g⁻¹), while lysine showed the lowest (2.6 g 100 g⁻¹). The brown oil extracted from the seed (yield 48.6 ± 0.94) had the following physicochemical properties; acid value, 3.05 ± 0.80 g, saponification value 166 ± 1.34 mg/KOH g⁻¹, free fatty acids, 0.3 g and peroxide value 3.02 ± 0.07 mg Eq kg⁻¹. The iodine value (80.1 ± 0.10)g 100 g⁻¹ indicated a preponderance of unsaturated fatty acid. Four fatty acids were detected whilst unsaturated acids constituted 61.3 g. Triglyceride was the dominant lipid species while hydrocarbons, waxes, sterols and sterol esters and higher alcohols, were detected in the unsaponifable matter. Results of nutritionally valuable mineral elements indicated that potassium occurred at the highest concentration.     

Acid and moisture uptake in steamed and boiled sweet potatoes and associated structural changes during in vitro gastric digestion

Orange-fleshed sweet potatoes are a good source of phytochemicals. For these nutrients to be absorbed, they must be released from the food matrix as a result of physical and chemical breakdown. A key factor in food breakdown is gastric acid diffusion into the matrix, and its influence on structural changes. Cooking treatment may influence mass transport properties and structural changes of foods during digestion. The objective of this study was to determine the acid and moisture uptake into sweet potatoes and its influence on macro- and micro-structures during in vitro gastric digestion as a result of varying cooking treatments. Sweet potatoes were cut into cubes and cooked (boiled or steamed) for different times. In vitro oral and gastric digestions were simulated in a shaking water bath at 37 °C. Acidity, moisture content, and solid loss were measured after 9 digestion times (15 to 240 min). Hardness of individual cubes and microstructure (light microscopy) were completed before and after digestion. Effective diffusivity of acid and moisture into the cubes was estimated using MATLAB. Cooking method, cooking severity, and digestion time significantly influenced moisture uptake (p < 0.0001). Acid uptake was significantly influenced by digestion time (p < 0.0001). The change of softening after digestion was influenced by cooking method and severity (p < 0.05). Effective diffusivity of acid ranged from 0.03 × 10^− 10 (mild steamed) to 11.40 × 10^− 10 m²/s (severely boiled). Percent texture decrease after digestion from the initial hardness ranged from 16% (severely steamed) to 34% (mild boiled). Textural changes were related to cell wall breakdown and starch degradation. In general, mass transport properties and macro- and microstructural changes were influenced by cooking treatment and gastric digestion. The link between food cooking and behavior during digestion is crucial in determining optimal food processing and cooking methods for specific food functional properties.     

Evaluation of Chemical Properties of Mistletoe Leaves from Three Different Trees (Avocado,African Oil Bean and Kola)

A comparative evaluation of the chemical composition of mistletoe leaves (Viscum album) growing on three different trees: avocado pear (Persea Americana), African oil bean (Pentaclethra macrophylla) and kola (Kola nitida) was undertaken. Fresh mistletoe leaves were obtained from the three different trees and thoroughly washed with water after which they were dried at room temperature (28 ± 1 °C) for 18 days during the dry season (December). After, the dried leaves were shredded and packaged. The samples were then subjected to phyto-chemical, vitamin and mineral analyses using standard methods of analyses. Anthocyanins ranged between 0.3.4 ± 0.003 and 0.37 ± 0.181 mg/g, Tannin, 2.09 ± 1.141 and 3.24 ± 0.003 mg/g, Chlorophyll a, 0.38 ± 0.004 and 0.39 ± 0.018 mg/g, Chlorophyll b 0.52 ± 0.120 and 0.60 ± 0.001 mg/g. Vitamin B1 ranged between 0.85 ± 0.038 and 0.94 ± 0.001 mg/g, Vitamin B2, 0.55 ± 0.007 and 0.61 ± 0.003 mg/g, Vitamin C, 0.77 ± 0.003 and 1.98 ± 0.003 mg/g, Folate 0.53 ± 0.004 and 0.58 ± 0.004 mg/g, Magnesium ranged between 0.21 ± 0.002 and 0.92 ± 0.003 mg/g, Calcium 2.14 ± 0.004 and 2.26 ± 0.001 mg/g, Sodium 0.01 ± 0.000. Iron, 1.24 ± 0.005 and 1.42 ± 0.006 mg/g. Sample obtained from oil bean tree showed lower and differed significantly (p < 0.05) in Anthocyanin and Chlorophyll b with samples obtained from avocado and kola trees. The sample obtained from avocado tree showed higher Tannin content compared with samples obtained from oil bean and kola trees (P ≤ 0.05). There were significant differences (P < 0.05) between the samples in all the vitamins. The minerals showed significant differences in the samples (P < 0.05) except in sodium content which was very low in all the samples. Mistletoe leaves are shown to have a rich chemical composition and could thus serve as a source of these phyto-chemicals, vitamins and minerals.     

Blood pressure-lowering properties of chokeberry (Aronia mitchurinii,var. Viking)

The blood pressure-lowering properties of lyophilized chokeberry juice and polyphenols were monitored using in vitro angiotensin-converting enzyme (ACE) inhibition measurement and a 10 day in vivo study with spontaneously hypertensive rats (SHR). Juice and polyphenols indicated weak ACE-inhibitory activity. The IC₅₀ values for polyphenols and juice were 1.5–2.5 and 4.5 mg dry matter/ml, respectively. In the SHR study the blood pressure-lowering effects of juice and polyphenol extract seemed to be short-term and were generally highest after 3 h from administration (50 mg/kg/day) when mean reductions in systolic blood pressure were 20 ± 8 and 15 ± 7 mm Hg, respectively. Corresponding mean decreases in diastolic blood pressure were 23 ± 6 and 13 ± 2 mm Hg in juice and polyphenol groups, respectively. It was concluded that both chokeberry juice and polyphenols had blood pressure-lowering effects. We hypothesize that chokeberry polyphenols enhance endothelial nitric oxide production with an ACE-independent mechanism, e.g. by activation of endothelial nitric oxidase enzyme; this is yet to be verified.     

Acceleration of mass transfer rates in osmotic dehydration of elephant foot yam (Amorphophallus paeoniifolius) applying pulsed-microwave-vacuum

The elephant foot yam slices were processed with combined pulsed-microwave-vacuum osmotic drying. Osmotic dehydration at ambient (28 °C and 45% RH) was carried out using different levels of sucrose concentration (30, 40, 50 and 60% w/w), salt concentration (5, 7.5, 10 and 12.5% w/w) and dehydration time (10, 20, 30, 50, 70, 90 and 120 min). During the osmotic dehydration, pulsed microwave vacuum (15 kPa pressure, 1 W/g power density and 1.853 pulsating ratio) was maintained for 2 min over the sample and solution to enhance the mass transfer. For this purpose, the osmotic dehydration experiments were conducted in the microwave-vacuum cavity. Azuara model predicted the moisture loss and solid gain by elephant foot yam slices during osmosis. It was observed that both the moisture loss and the solid gain increased with increasing concentration of the osmotic solution. The optimum conditions found in the process were 40% w/w sucrose concentration, 6% w/w salt concentration and 70 min osmotic dehydration time, resulting in to 42.80% moisture loss (initial weight) and 14.65% solid gain (initial weight). Further, samples were dried using microwave vacuum dryer up to moisture content of 5–6% d.b. by varying microwave power density (2, 4, 6 and 8 W/g) and pulsating ratio (1.312, 1.625, 1.983 and 2.250). Page model was fitted to the data to study the microwave vacuum drying kinetics. The microwave vacuum drying at 1.625 pulsating ratio with microwave power density 4 W/g yielded a product with the highest overall acceptability score. Guggenheim, Anderson and deBoer (GAB) model was used in the study of the sorption behavior of dehydrated elephant foot yam and shelf life prediction.Industrial relevanceThe production of elephant foot yam in India and South East Asia is comparatively higher than other vegetables. Although, it is nutritious product and good source of energy, food industries are not interested to process elephant foot yam using a time consuming traditional osmotic dehydration process followed by hot air drying. Therefore, present research work was undertaken from industry suggestion to develop accelerated osmotic dehydration process for elephant foot yam using novel pulsed-microwave-vacuum combination followed by finish drying by microwave-vacuum. This research has been carried out to decrease industrial processing time, energy consumption and improving quality of the product. Industry will start adopting this new hybrid process of drying elephant foot yam on large scale.     

Selective enrichment of dairy phospholipids in a buttermilk substrate through investigation of enzymatic hydrolysis of milk proteins in conjunction with ultrafiltration

Extensive enzymatic hydrolysis of milk proteins in reconstituted buttermilk powder was combined with ultrafiltration to generate a phospholipid (PL) enriched fraction with maximum permeation of hydrolysed peptides. Buttermilk, naturally high in PLs, is the ideal substrate for enrichment of these bio- and techno-functionally active compounds. A 7.8 fold increase in PL was achieved in the 50 kDa retentate; 6.16 ± 0.02% total PL compared with 0.79 ± 0.01% in the starting substrate, an increase considerably greater than previously reported. Total lipid content (% dry matter) increased 6.3 fold in the retentate, 43.43 ± 0.61%, from the starting substrate, 6.84 ± 0.17%. This combined strategic approach enabled maximum enrichment of PLs with no transmission of lipid material into the permeate, 0.09 ± 0.02% total lipid, and non-detectable levels of PLs recovered in the permeate, 0.00 ± 0.01% total PL.     

An Automated Cassava Peeling System for the Enhancement of Food Security in Nigeria

Cassava is second only to sweet potato as the most important starchy root crop of the tropical world. In most parts of the tropics, cassava is grown on small plots. However, in some countries, e.g. Mexico, Brazil and Nigeria, large plantations have been started and interest in mechanization is growing. The degree of mechanization depends on the size of the land and availability of machines for each unit operation involved in cassava processing. However, of all the unit operations involved in cassava processing, cassava peeling remains a serious global challenge to process engineers. An automated cassava peeling machine was thus developed in the Department of Agricultural Engineering, Federal University of Technology, Akure. The design of the machine was based on the development and modification of the peeling tool of previous cassava peeling machines and the peeling principle is by impact. This machine was evaluated using newly harvested improved variety of cassava tuber (TMS 30572) grouped into different sizes: 100 ≤ L < 140, 140 ≤ L < 180, 180 ≤ L < 220, 220 ≤ L < 260, and 260 ≤ L < 300. The speed of the machine varies from 100 – 600 rpm while machine operational parameters such as throughput capacity, peeling efficiency, mechanical damage, quality performance efficiency and peel retention were determined for each size. Results revealed that throughput capacity ranged from 76 – 442 kg/h, peeling efficiency ranged from 50 – 75%, mechanical damage ranged from 12 – 44%, quality performance efficiency ranged from 22 – 66%, and peel retention ranged from 7.2 – 33%. These results show appreciable improvement over manual method and existing machines.     

Effect of corona wind,current, electric field and energy consumption on the reduction of the thawing time during the high-voltage electrostatic-field (HVEF) treatment process

Electric energy plays an important role in reducing the thawing time and accelerating the entire thawing process. Corona wind speed was increased by increasing the applied voltage and current, which were 0.87 ± 0.19, 1.22 ± 0.19, 1.49 ± 0.13 m/s under 8, 10, 12 kV with 3, 5, 10 μA; 1.01 ± 0.12, 1.24 ± 0.17, 1.49 ± 0.10 m/s under − 8, − 10, − 12 kV with 5, 9, 14.5 μA. The models T1, T2, and T3 were designed to study the effects of current, corona wind and electric field on thawing, respectively. T1: a thin stainless-steel sheet covered the top of stainless-steel box with 10 g frozen distilled water; T2: a plastic sheet was placed under the stainless-steel box; T3: a plastic sheet covered the top of plastic box. Electric field alone cannot affect thawing time, which could be maximally reduced by 1/2 compared to that of air thawing.Industrial relevanceWe have studied HVEF thawing for several years because of its many advantages, such as the quick thawing of frozen meat with little energy consumption and good post-thawing quality. The mechanism of HVEF thawing and the reduction of the thawing time, which are important for the industrial application of new technology from principle to design, must be clarified. Meanwhile, this emerging technology will be beneficial for the food-thawing field.     

Thermo-mechanical properties of egg albumen–cassava starch composite films containing sunflower-oil droplets as influenced by moisture content

The effect of moisture content on the thermo-mechanical and structural properties of egg albumen–cassava starch composite films containing sunflower oil droplets was studied using dynamic mechanical analysis (DMA), differential scanning calorimetry (DSC) and scanning electron microscopy (SEM). Composite films were prepared by cold gelation, dried in a moisture controlled incubator (83.5%RH) at 25 °C for 8 days and aged at different relative humidity at room temperature (21 ± 1 °C) for 7 days to obtain composite films with moisture contents of 4%, 7%, 11%, 17% and 46% (dry weight basis). In DMA thermograms the magnitude of G′ and G″ increased with increasing temperature in high-moisture samples, decreased and then again gradually increased for intermediate-moisture samples, and decreased in low moisture samples. DSC thermograms indicated two distinct peaks (at 49–53 °C and 79.8 to 132.4 °C) which were attributed to phase transitions and protein denaturation. SEM images indicated that the microstructure of the composite matrix changed with moisture content and heating temperature. Our study confirms that moisture content plays a key role in the thermo-mechanical properties and microstructure of egg albumen–cassava starch composite films containing sunflower oil.     

Phytochemical,antioxidant and pigment stability of açai (Euterpe oleracea Mart.) as affected by clarification,ascorbic acid fortification and storage

Açai juice at two clarity stages (semi-clarified and clarified) was compared to 100% açai pulp following ascorbic acid fortification to evaluate phytochemical and antioxidant changes during storage at 4 and 20 °C. Cyanidin-3-rutinoside (202 ± 5.8 mg/L) and cyanidin-3-glucoside (75 ± 4.8 mg/L) were the predominant anthocyanins in açai while 11 non-anthocyanin polyphenolics were detected in concentrations from 1.1 to 55 mg/L of açai pulp. Clarification of açai pulp resulted in a 27% loss in total polyphenolics (197 ± 6.9 mg gallic acid/100 mL) and in a 20% reduction in both total anthocyanins (729 ± 3.4 mg/L) and antioxidant capacity (54 ± 1.7 μmol Trolox equivalents/mL). Anthocyanin degradation followed first order kinetics, with half-lives ranging from 9.4 to 43 days for cyanidin-3-glucoside and from 18 to 82 days for cyanidin-3-rutinoside. Fortification with ascorbic acid accelerated anthocyanin degradation in clarified juice at both storage temperatures, likely due to the loss of polymeric anthocyanin forms (21%) during clarification. Although clarification enhanced the amount of monomeric anthocyanins present in açai juice which relates positively to the aesthetic quality, processing and handling regimes must be optimized to achieve maximum retention of their functional properties during storage.     

Biochemical changes throughout the ripening of a traditional Spanish goat cheese variety (Babia-Laciana)

The physico-chemical characteristics, proteolysis (classical nitrogen fractions, caseins and their degradation products and free amino acids), and lipolysis (fat acidity and free fatty acids) were studied throughout the ripening of three batches of Babia-Laciana cheese, a Spanish traditional variety made from raw goats’ milk. The main compositional characteristics of this cheese at the end of the ripening are its high content of total solids (TS) (78.0±2.4 g 100 g⁻¹ of cheese) and fat (61.1±1.2 g 100 g⁻¹ of TS), the presence of residual lactose (1.6±0.8 g 100 g⁻¹ of TS) and its low content of sodium chloride (1.1±0.7 g 100 g⁻¹ of TS) and ash (2.8±0.5 g 100 g⁻¹ of TS). Its pH values (4.44±0.72) are extraordinarily low. The evolution and final values of the different nitrogen fractions show that this cheese undergoes a very slight proteolysis, a fact which was corroborated when the caseins and their degradation products were quantified: β-casein did not undergo any modification throughout ripening, while only 21% of the α_s-caseins were degraded. Free amino acids content increased by a factor of about 7 throughout ripening, resulting in a high content of γ-amino butyric acid and a low content of glutamic acid at the end of the process. Fat acidity increased very slightly, approximately 4.5 times, during ripening, reaching final values of 3.5±2.2 mg KOH g⁻¹ of fat. The total free fatty acids content showed a similar evolution to fat acidity. At the end of the ripening process, the main free fatty acid was C_18:1, followed by C₁₆ and C₁₀.