Evaluation of the relation between Pavlovian occasion-setting and instrumental discriminative stimuli: A blocking analysis.

Retracted October 1990. (See record 1991-03475-001.) Conducted 2 experiments with 72 male Sprague-Dawley rats, using a blocking design (A+, then AB+) to assess the relation between Pavlovian occasion-setting and instrumental discriminative stimuli. Prior conditioning of both associative and occasion-setting functions of A in a serial feature-positive procedure blocked acquisition of an instrumental discriminative function by a novel stimulus (B) trained in compound with A. Neither prior conditioning of only an A/unconditioned stimulus/stimuli (UCS) association nor prior conditioning of Stimulus A using a Pavlovian simultaneous feature-positive procedure, which does not endow A with an occasion-setting function, blocked acquisition of an instrumental discriminative function by B. Prior acquisition of an instrumental discriminative function by A blocked acquisition of a Pavlovian occasion-setting function by a novel stimulus (B) trained in compound with A but did not block acquisition of an association between B and the UCS. (39 ref) (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Role of within-compound associations in occasion setting: A blocking analysis.

Retracted October 1990. (See record 1991-03475-001.) In two experiments with rat subjects we evaluated the relation between within-compound associations and occasion setting established during feature-positive discriminations. Various treatments preceded serial feature-positive discrimination training in which A and B served as the feature, and X as the element common to reinforced and nonreinforced trials. In Experiment 1, prior serial feature-positive training using A as the feature blocked acquisition of an association between the novel B feature and the unconditioned stimulus (US), an association between the novel B and the common element (X), and the occasion-setting function of B. However, prior simultaneous feature-positive training using A as the feature blocked an association between the novel B and the US and between B and X but did not block acquisition of an occasion-setting function by B. In Experiment 2, serial nondifferential conditioning endowed the A stimulus with the capacity to block acquisition of a B–US association and a within-compound association between B and X but did not prevent acquisition of occasion setting to B. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Impaired classical eyeblink conditioning in cerebellar-lesioned and Purkinje cell degeneration (pcd) mutant mice

Converging lines of evidence from rabbits, rats, and humans argue for the crucial involvement of the cerebellum in classical conditioning of the eyeblink/nictitating membrane response in mammals. For example, selective lesions (permanent or reversible) of the cerebellum block both acquisition and retention of eyeblink conditioning. Correspondingly, electrophysiological and brain-imaging studies indicate learning-related plasticity in the cerebellum. The involvement of the cerebellum in eyeblink conditioning is also supported by stimulation studies showing that direct stimulation of the two major afferents to the cerebellum (the mossy fibers emanating from the pontine nucleus and climbing fibers originating from the inferior olive) can substitute for the peripheral conditioned stimulus (CS) and unconditioned stimulus (US), respectively, to yield normal behavioral learning. In the present study, we examined the relative contribution of the cerebellar cortex versus deep nuclei (specifically the interpositus nucleus) in eyeblink learning by using mutant mice deficient of Purkinje cells, the exclusive output neurons of the cerebellar cortex. We report that Purkinje cell degeneration (pcd) mice exhibit a profound impairment in the acquisition of delay eyeblink conditioning in comparison with their wild-type littermates. Nevertheless, the pcd animals did acquire a subnormal level of conditioned eyeblink responses. In contrast, wild-type mice with lesions of the interpositus nucleus were completely unable to learn the conditioned eyeblink response. These results suggest that both cerebellar cortex and deep nuclei are important for normal eyeblink conditioning.     

Transfer across unconditioned stimuli in serial feature discrimination training.

The transfer of conditioned modulation across CS and unconditioned stimulus/stimuli (UCS) was examined in 3 experiments that used Pavlovian appetitive training procedures with rats. In Exp 1, after training in a positive patterning discrimination (X→A+/X-/A-), X increased CR elicited by another trained-then-extinguished CS as long as that CS had been trained with the same UCS as was used in discrimination training. In Exp 2, after training with a feature-negative discrimination (X→A-/A+), X inhibited CR elicited by another trained-then-extinguished CS as long as that CS had been trained with the same UCS. Exps 1 and 2 used a between-groups design, and Exp 3 used a within-groups design. In Exp 3, rats were trained in a feature-positive discrimination (X→A+/A-). In transfer tests, X increased CR elicited by another CS trained then extinguished with the same UCS from training. This increase was greater than the X increased CR elicited by another CS trained then extinguished with a different UCS from training. Results supported the suggestion that features trained in serial discrimination tasks influence behavior indirectly by transiently raising or lowering the threshold for activation of the UCS representations by its target stimuli and by any other stimuli that may be associated with that UCS. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Blocking during serial feature-positive discriminations: Associative versus occasion-setting functions.

Previous work indicates that during serial feature-positive discriminations (i.e., feature–trace interval/common element/food presentations and separate nonreinforced common element presentations) the feature (A) acquires an association with food (unconditioned stimulus [UCS]) and also a capacity to modulate the common element (X)–UCS relation, which has been termed occasion setting (OS). In the present 2 experiments with 32 male Sprague-Dawley rats, a blocking design, serial feature-positive discrimination procedures, and a behavioral observation technique were used to assess the relation between associative and OS functions acquired by stimuli. In Exp I, prior conditioning of associative and OS functions to A blocked acquisition of both stimulus functions by a novel stimulus (B) trained in compound with A. In Exp II, prior acquisition of an A–UCS association blocked acquisition of a B–UCS association but had no effect on acquisition of B's OS function. These outcomes are discussed (a) as indicating that associative and OS functions are independent and may be based on separate learning processes, (b) as extending the known conditions for acquisitions of OS, and (c) with regard to the theoretical implications of assuming independence of associative OS functions acquired by stimuli. (28 ref) (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Early and late acquisition of discriminative neuronal activity during differential conditioning in rabbits: Specificity within the laminae of cingulate cortex and the anteroventral thalamus.

Recorded multiple-unit activity of the circulate cortex and the anteroventral (AV) nucleus of the thalamus during discriminative conditioning of an avoidance response (locomotion) in 50 New Zealand White rabbits. Results indicate a greater unit response in cingulate cortex to the positive conditional stimulus (CS+; a tone paired with a footshock UCS) relative to the negative conditional stimulus (CS–; a tone randomly interspersed with the positive stimuli but never paired with the UCS). The majority of neuronal records obtained from the deep laminae of cingulate cortex manifested 1st neuronal discrimination in the session of 1st exposure to conditioning. However, the majority of neuronal records of the superficial laminae showed 1st discrimination at a late stage of training, during the session in which the criterion of behavioral discrimination was met. The late developing discriminative activity of the superficial laminae was coincident with the late developing discriminative activity of the AV thalamus. Once acquired, neuronal discrimination in cortex persisted throughout 600 msec after CS onset, and during 6 sessions of training of overtraining. Analysis of individual neuronal records suggested that the persistence during overtraining resulted from replacement of early fading neuronal discriminations by late neuronal discriminations. (44 ref) (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Awareness in classical differential eyeblink conditioning in young and aging humans.

The role of awareness and its impact on learning the conditioned eyeblink response was investigated in both trace and delay discrimination eyeblink conditioning in young and aging participants, in 4 paradigms: delay 750, delay 1250, trace 500, and trace 1000. Participants concurrently watched a silent movie about which they were questioned afterward. Acquisition in both the trace and delay discrimination task was correlated with awareness of conditioning stimulus contingencies, regardless of age. Age-dependent deficits were observed in trace discrimination but not in delay discrimination, with more severe deficits appearing at the longer trace interval. The percentage of aware participants was also found to be greater in the young population than in the aging population. These results indicate that awareness or knowledge of stimulus contingencies may be an important contributor to successful acquisition in higher order discrimination tasks. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Motor cortex lesions do not affect learning or performance of the eyeblink response in rabbits.

The possible modulatory role of motor cortex in classical conditioning of the eyeblink response was examined by ablating anterior neocortex in rabbits and training them with an auditory conditioned stimulus (CS) and an airpuff unconditioned stimulus (US) in either a delay (Experiment 1) or a trace (Experiment 2) conditioning paradigm. Topographic measures such as amplitude and onset latency were assessed during conditioning sessions for conditioned responses (CRs) and on separate test days for unconditioned responses (URs) by using a range of US intensities. No lesion effects were observed for learning or performance measures in acquisition or retention of either delay or trace conditioning. During trace conditioning, lesioned rabbits did, however, exhibit a trend toward impairment and demonstrated significantly longer CR latencies. Damage to motor and frontal cortex does not significantly affect eyeblink response performance or learning in either a delay or a trace conditioning paradigm. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Eyeblink conditioning during an interstimulus interval switch in rabbits (Oryctolagus cuniculus) using picrotoxin to disrupt cerebellar cortical input to the interpositus nucleus.

The role of the cerebellar cortex in eyeblink classical conditioning remains unclear. Experimental manipulations that disrupt the normal function impair learning to various degrees, and task parameters may be important factors in determining the severity of impairment. This study examined the role of cerebellar cortex in eyeblink conditioning under conditioned stimulus?unconditioned stimulus intervals known to be optimal or nonoptimal for learning. Using infusions of picrotoxin to the interpositus nucleus of the rabbit cerebellum, the authors pharmacologically disrupted input from the cerebellar cortex while training with an interstimulus interval (ISI)-switch procedure. One group of rabbits (Oryctolagus cuniculus) was 1st trained with a 250-ms ISI (optimal) and then switched to a 750-ms ISI (nonoptimal). A 2nd group was trained in the opposite order. The most striking effect was that picrotoxin-treated rabbits initially trained with a 250-ms ISI learned comparably to controls, but those initially trained with a 750-ms ISI were severely impaired. These results suggest that functional input from cerebellar cortex becomes increasingly important for the interpositus nucleus to learn delay eyeblink conditioning as the ISI departs from an optimal interval. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Cerebellar cortical degeneration disrupts discrimination learning but not delay or trace classical eyeblink conditioning.

The authors investigated classical eyeblink conditioning in a relatively rare patient, B.R., with extensive cerebellar cortical atrophy and marked sparing of the dentate nucleus. Patient B.R.'s ability to acquire and extinguish simple associations (delay and trace conditioning tasks) as well as her ability to acquire more complex associations (temporal and simple discrimination tasks) were examined. There are 2 primary findings from this study. First, B.R. showed normal acquisition and extinction in delay and trace conditioning. Second, she demonstrated a complete inability to learn associative discriminations, even in the case of a simple 2-tone discrimination within the context of a delay paradigm. The latter finding was unexpected because of the sparing of her deep cerebellar nuclei. These data suggest that the cerebellar cortex, or pathways traversing cerebellar cortex, play an important role in classical eyeblink discrimination learning. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Relationships between the determinants of performance in serial feature-positive discriminations.

Conducted 3 experiments using an appetitive behavioral observation procedure to investigate the effects of pre- and postdiscrimination treatments on learning and performance in serial feature-positive discriminations. Previous work suggests that performance in this discrimination is based on feature–UCS associations, feature–common element associations, and a conditional occasion-setting relation. Exp I examined the effects of prediscrimination reinforcement of the components of the discrimination. 26 male and 6 female Sprague-Dawley rats were assigned, in groups of 8, to 1 of 4 prereinforcement conditions (involving administration of a tone, a light, and a trace interval). Ss then trained on a serial feature-positive discrimination. Behavioral observations were recorded. Exps II and III investigated the effects of postdiscrimination nonreinforcement and reinforcement procedures on performance of established discriminative responding by 64 Ss. Results of the studies and of previous work are interpreted to show that the conditional occasion-setting relation may be independent of feature–UCS associations and may not depend on feature/common element associations. (61 ref) (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Lesions of the rat perirhinal cortex spare the acquisition of a complex configural visual discrimination yet impair object recognition.

Rats with perirhinal cortex lesions were sequentially trained in a rectangular water tank on a series of 3 visual discriminations, each between mirror-imaged stimuli. When these same discriminations were tested concurrently, the rats were forced to use a configural strategy to solve the problems effectively. There was no evidence that lesions of the perirhinal cortex disrupted the ability to learn the concurrent configural discrimination task, which required the rats to learn the precise combination of stimulus identity with stimulus placement (“structural” learning). The same rats with perirhinal cortex lesions were also unimpaired on a test of spatial working memory (reinforced T maze alternation), although they were markedly impaired on a new test of spontaneous object recognition. For the recognition test, rats received multiple trials within a single session in which on every trial, they were allowed to explore 2 objects, 1 familiar, the other novel. On the basis of their differential exploration times, rats with perirhinal cortex lesions showed very poor discrimination of the novel objects, thereby confirming the effectiveness of the surgery. The discovery that bilateral lesions of the perirhinal cortex can leave configural (structural) learning seemingly unaffected points to a need to refine those models of perirhinal cortex function that emphasize its role in representing conjunctions of stimulus features. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Effects of Age and Awareness on Eyeblink Conditional Discrimination Learning.

The present study aimed to assess the effects of aging and awareness on conditional discrimination learning within an eyeblink conditioning procedure by using a consecutive age-groups design (20-35 years, 36-50 years, 51-65 years, 66-80 years). Increasing age was associated with a decline in overall eyeblink conditioned response (CR) frequency and a deficit in conditional discrimination learning in the 2 older groups. Awareness of stimulus contingencies affected discrimination performance but not overall CR rates in younger subjects. Older subjects did not achieve eyeblink conditional discrimination learning, regardless of awareness. Discrimination performance correlated with measures of declarative memory. The pattern of results is discussed with respect to the involvement of hippocampal-cerebellar interactions and awareness in the mediation of age-related conditioning changes. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Purkinje Cell Loss by OX7-Saporin Impairs Excitatory and Inhibitory Eyeblink Conditioning.

Cerebellar cortical contributions to eyeblink conditioned excitation have been examined extensively. In contrast, very little evidence exists concerning the role of the cerebellar cortex in eyeblink conditioned inhibition. In the current study, rats were given intraventricular infusions of the immunotoxin OX7-saporin to selectively destroy Purkinje cells throughout the cerebellar cortex following excitatory conditioning. After a 2-week postinfusion period, the rats were given reacquisition training. After reacquiring excitatory conditioning, the rats were trained in a feature-negative discrimination procedure to establish conditioned inhibition. Rats treated with OX7-saporin showed impaired reacquisition of excitatory conditioning and acquisition of conditioned inhibition. The results suggest that Purkinje cells play important, but different, roles in conditioned excitation and inhibition in rats. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Retraction of Ross and LoLordo findings concerning blocking in serial feature-positive discriminations.

Findings concerning the effectiveness of stimuli from various conditioning procedures in blocking conditioned excitation and occasion-setting functions of an added stimulus in a serial feature-positive discrimination training procedure (V. M. LoLordo and R. T. Ross; see record 1987-24106-001; Ross and LoLordo; see record 1987-14728-001) are retracted. Videotapes on which the published data were based were rescored by 2–5 people, most of whom were uninformed about group memberships of the Subjects. In no case did the rescoring confirm any of the original findings of blocking. Possible factors contributing to the discrepancies are discussed. The experiments should be repeated with feature stimuli that are less similar to each other and with several scorers, at least one of whom is unaware of the group assignment of the Subjects. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Contributions of postrhinal and perirhinal cortex to contextual information processing.

The role of the postrhinal cortex (POR) and the perirhinal cortex (PER) in processing relational or contextual information was examined with Pavlovian fear conditioning. Rats with electrolytic or neurotoxic lesions of the POR or PER were tested in 2 contextual fear conditioning paradigms. In Experiment 1, electrolytic lesions of the POR or PER produced impairments in contextual fear conditioning but not in conditioning to a phasic auditory conditioned stimulus. Neurotoxic lesions of the POR or PER likewise resulted in anterograde (Experiment 2) and retrograde (Experiment 3) deficits in fear conditioning to the training context in an unsignaled shock paradigm. The results suggest that operations performed on sensory information by the POR and PER are necessary to support contextual learning. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Correction to Fortier et al. (2008).

Reports an error in "Delay discrimination and reversal eyeblink classical conditioning in abstinent chronic alcoholics" by Catherine Brawn Fortier, Elizabeth M. Steffen, Ginette LaFleche, Jonathan R. Venne, John F. Disterhoft and Regina E. McGlinchey (Neuropsychology, 2008[Mar], Vol 22[2], 196-208). The lifetime drinking data listed in Table 1 on p. 198 was not correctly calculated and underestimated lifetime exposure to alcohol. The corrected lifetime variables from that table are included. (The following abstract of the original article appeared in record 2008-02526-007.) Evidence has shown that alcoholism leads to volume reductions in brain regions critical for associative learning using the eyeblink classical conditioning paradigm (EBCC). Evidence indicates that cerebellar shrinkage causes impairment in simple forms of EBCC, whereas changes in forebrain structures result in impairment in more complex tasks. In this study, the ability of abstinent alcoholics and matched control participants to acquire learned responses during delay discrimination and discrimination reversal was examined and related to severity of drinking history and neuropsychological performance. During discrimination learning, one tone (CS+) predicted the occurrence of an airpuff (unconditioned stimulus), and another tone (CS-) served as a neutral stimulus; then the significance of the tones was reversed. Alcoholics who learned the initial discrimination were impaired in acquiring the new CS+ after the tones reversed; this is a function that has previously been linked to forebrain structures. It is suggested that a factor important to alcoholic addiction may be the presence of alcoholic-related associative responses that interfere with the ability to learn new more adaptive associations. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Eyeblink classical conditioning: Hippocampal formation is for neutral stimulus associations as cerebellum is for association–response.

Extensive evidence has been amassed that the cerebellum, hippocampus, and associated circuitry are activated during classical conditioning of the nictitating membrane/eyeblink response. In this article, the authors argue that the cerebellum is essential to all eyeblink classical conditioning paradigms. In addition, the septohippocampal system plays a critical role when the classical conditioning paradigm requires the formation of associations in addition to the simple association between the conditioned and unconditioned stimuli. When only a simple conditioned stimulus–unconditioned stimulus association is needed, the septohippocampal system has a more limited, modulatory role. The neutral stimulus association versus simple association–response distinction is one of the ways in which declarative or relational memory can be separated from nondeclarative or nonrelational memory in classical conditioning paradigms. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Medial Prefrontal Lesions and Pavlovian Eyeblink and Heart Rate Conditioning: Effects of Partial Reinforcement on Delay and Trace Conditioning in Rabbits (Oryctolagus cuniculus).

Effects of continuous (100%) versus partial (25%) reinforcement were studied on Pavlovian delay and trace eyeblink conditioning in rabbits (Oryctolagus cuniculus) with either lesions to the medial prefrontal cortex (mPFC) or sham lesions. Concomitant heart rate changes evoked by the conditioned stimulus were also assessed. Partial reinforcement retarded eyeblink conditioning in both the trace and delay paradigm, but this impairment was greater during trace conditioning and in rabbits with mPFC lesions. Accompanying conditioned stimulus-evoked heart rate slowing was attenuated under all conditions by the mPFC lesions, although this result was not always statistically significant. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Fear conditioning to discontinuous auditory cues requires perirhinal cortical function.

Pretraining lesions of rat perirhinal (PR) cortex impair fear conditioning to ultrasonic vocalizations (USVs) but have no effect on conditioning to continuous tones. This study attempted to deconstruct USVs into simpler stimulus features that cause fear conditioning to be PR-dependent. Rats were conditioned to one of three cues: a multicall 19-kHz USV, a 19-kHz discontinuous tone, and a 19-kHz continuous tone. The discontinuous tone duplicated the on/off pattern of the individual calls in the USV, but it lacked the characteristic frequency modulations. Well-localized neurotoxic PR lesions impaired conditioning to the USV, the discontinuous tone, and the training context. However, PR lesions had no effect on conditioning to the continuous tone. The authors suggest that the lesion effects on fear conditioning to both cues and contexts reflect the essential role of PR in binding stimulus elements together into unitary representations. (PsycINFO Database Record (c) 2010 APA, all rights reserved)