酒曲应用于菊芋发酵的初步研究

菊芋是一种极具潜力的乙醇发酵原料,与淀粉质原料相比,菊芋低温条件下即可实现糊化,在节能方面具有优势。本文选取了安琪甜酒曲、安琪酿酒曲和欣马酒业酒曲进行一步法发酵实验,论证不同种类及品种的酒曲应用于菊芋发酵生产乙醇的可行性。结果表明:在原料含糖量不高且未经任何条件优化的情况下,安琪酿酒曲的酒度高于其他两种酒曲,酒度可达5.5度;发酵过程中,安琪酿酒曲的CO2失重量始终高于安琪甜酒曲和欣马酒曲;使用安琪酿酒曲进行发酵,第1d到第3d乙醇含量逐渐升高,在第3d时达到最大值;随着发酵时间的继续延长,安琪酿酒曲发酵液pH为6.35,发酵1d后pH降至4.82,3d后pH降至4.48,pH趋势稳定。从本实验的结果可推论,与其他两种酒曲相比,安琪酿酒曲更为适用于菊芋发酵生产乙醇。     

复合酶在菊芋粉发酵生产燃料乙醇中的应用

菊芋是一种可选择的酒精发酵原料.研究用菊芋粉生产燃料乙醇的工艺参数,在单因素试验的基础上用正交试验进行工艺参数的优化.确定最佳工艺条件为:料水比为1:4.5(m/v),复合酶添加量0.2%,发酵温度36℃,酵母液用量为6mL,发酵pH值为5.0,发酵时间24 h.在此工艺条件下酒精度可达10.8%voi,酒精产率达34.10%.技术经济分析说明,使用复合酶和酵母发酵菊芋粉生产燃料乙醇是可行的.     

膨化木薯发酵制取燃料乙醇

为了探讨使用酿酒曲发酵膨化木薯生产燃料乙醇的最优发酵条件,利用膨化技术对木薯进行了预处理,并用响应面法优化了发酵温度、液固比、糖化酶用量。结果表明:利用膨化处理缩短了乙醇生产周期,降低能源消耗,提高了生产效率。最适的发酵条件为:温度32℃、发酵初始pH值4.5、糖化酶用量145 U/g、液固比2.8∶1(mL∶g)、酿酒曲用量0.3%、发酵时间72 h。在此条件下,膨化木薯最终酒精度达到了11.94%。     

安琪纯种曲在大曲清香型白酒生产中的应用研究

主要研究安琪根霉曲、香霸、酿酒曲等纯种微生物制剂在大曲清香型白酒中的应用。以宣酒大曲清香型工艺为基础,研究根霉菌在前期糖化,香霸在大米查和二米查发酵,酿酒曲在二米查发酵中带来的变化。通过几种大曲清香工艺的对比,从发酵情况,产量、酒质、成本等方面综合比较。得出安琪纯种曲的应用,在配比与工艺合理的情况下,先进行培菌糖化,随后加入香霸、中温曲和偏高温曲进行大米查发酵,然后加入香霸、酿酒曲和偏高温曲进行二米查发酵。大米查酒和二米查酒分开贮存,精心组合勾兑,既改良了大曲清香型酒质,又提高了出酒率,节约了成本。     

应用安琪酿酒曲糖化工艺的研究

将安琪酿酒曲应用于高粱的糖化,采用响应面分析对安琪酿酒曲的各项糖化工艺参数进行优化研究,确定最佳糖化工艺条件为:加曲量a2、糖化时间b3、糖化初始温度c2、糖化糟厚度d2,在此糖化工艺条件下,出酒率达到54.25%±0.5%,出酒率得到明显提高。     

基于安琪混合酒曲的糯米酒新工艺研究

以安琪混合酒曲为发酵剂,对糯米酒的酿酒新工艺进行探究。以发酵酒液的酒精度、总酸、外观糖度、感官评分为评价指标,探究发酵时间、发酵温度、料液比、发酵剂添加量对酿造米酒的影响,在此基础上进行正交试验确定糯米酒的最佳酿造工艺。结果表明,发酵工艺条件为安琪混合酒曲（安琪甜酒曲:安琪纯种活性干酵母为4:1）添加量0.625%,料液比1.0:1.6（g:mL）,发酵温度30 ℃,发酵时间8 d。在此最佳工艺条件下,糯米酒的感官评分为92分,酒精度12.2%vol,外观糖度12.8%,总酸3.50 g/L。糯米酒酸甜适中,口感醇厚,具有独特的芳香。     

安琪超级耐高温酿酒高活性干酵母研制成功

本刊讯:专门针对酒精行业的浓醪发酵而开发的安琪超级耐高温酿酒高活性干酵母研制成功,其使用方便,能广泛适用于以淀粉质为原料的酒精生产。其革命性优势在于:1.能有效增强酵母在浓醪发酵过程中乙醇对酵母毒性的抗性,提高酵母发酵酶系列活力的钝化温度和酵酶系活力,进而提高发酵力和产酒能力。2.能有效降低浓醪发酵过程的饱和蒸汽压,解决发酵过程由葡萄糖效应引起的底物抑制效应,促使酵母细胞酶产乙醇向醪液中快速渗透。降低渗透压对酵母产乙醇的抑制,提高发酵能力和出酒率。3.安琪超级耐高温酿酒高活性干酵母含有丰富的营养物质,能满足高浓…     

安琪酿酒曲在小曲酒生产中的应用技术

安琪牌酿酒曲系用现代生物技术生产的多种活性微生物产品，它保留了传统酒曲中多种酶系糖化发酵的特点，更具有菌种纯、有效成分含量高、糖化发酵能力强等显著优点。以玉米、高粱、稻谷为原料，应用安琪酿酒曲，只要合理操作，即可获得很好的出酒率和较高的经济效益。(陶然)     

三种酵母用于火棘发酵果酒的对比研究

以安琪葡萄酒果酒酵母RW、安琪酿酒曲、LAFFORT F33葡萄酒酵母为发酵酵母和成熟的火棘果实为原料,经过主发酵、后发酵和澄清等工艺酿制成火棘发酵酒。经过对成品发酵果酒的感官和常规的理化指标的对比研究表明,LAFFORT F33酿造的火棘果酒质量稳定,酒色鲜红色、澄清透明、无沉淀;果香浓郁,酒香、果香协调;口感纯净、幽雅;典型性强,且理化检测指标均符合国家标准,是一种理想的适合生产火棘果酒的发酵酵母。     

安琪酿酒曲在红枣固态白酒发酵上的应用

安琪酿酒曲分别为投入高粱量0.2%、0.5%、0.8%的用量,在红枣固态白酒发酵上应用,通过对所产酒的产量、质量、发酵温度变化、堆积糖化、出窖糟醅化验五方面进行综合分析,得出结果:安琪酿酒曲用量为投入高粱量0.5%时,酒的产量高,质量相比优于其他用量比例。     

Reliable identification of grapevine rootstock varieties using RAPD PCR on woody samples

Since grapevine ( Vitis spp .) rootstock material is being traded increasingly as disbudded woody material a lack of distinctive morphological features on such material necessitates an alternative and reliable means of identification. Methods described here were developed for rapid and efficient extraction of DNA from woody samples rich in phenolic compounds and polysaccharides, and for subsequent identification of varieties by RAPD PCR. Using these methods, and with the application of only one selected RAPD primer, we were able to differentiate sixteen rootstock varieties, including the seven varieties most commonly used in Germany. Problems commonly encountered with reproducibility of RAPD patterns were avoided by choosing primers with a dinucleotide sequence and a high G/C content that allowed a rather high annealing temperature of 45°C. Methods described here should also be useful for other horticultural crops, especially those with woody tissues rich in phenolic compounds and polysaccharides.     

An internet compendium of analytical methods and spectroscopic information for monomers and additives used in food packaging plastics

An internet website (http://cpf.jrc.it/smt/) has been produced as a means of dissemination of methods of analysis and supporting spectroscopic information on monomers and additives used for food contact materials (principally packaging). The site which is aimed primarily at assisting food control laboratories in the European Union contains analytical information on monomers, starting substances and additives used in the manufacture of plastics materials. A searchable index is provided giving PM and CAS numbers for each of 255 substances. For each substance a data sheet gives regulatory information, chemical structures, physico-chemical information and background information on the use of the substance in particular plastics, and the food packaging applications. For monomers and starting substances (155 compounds) the infra-red and mass spectra are provided, and for additives (100 compounds); additionally proton NMR are available for about 50% of the entries. Where analytical methods have been developed for determining these substances as residual amounts in plastics or as trace amounts in food simulants these methods are also on the website. All information is provided in portable document file (PDF) format which means that high quality copies can be readily printed, using freely available Adobe Acrobat Reader software. The website will in future be maintained and up-dated by the European Commission's Joint Research Centre (JRC) as new substances are authorized for use by the European Commission (DG-ENTR formerly DGIII). Where analytical laboratories (food control or other) require reference substances these can be obtained free-ofcharge from a reference collection housed at the JRC and maintained in conjunction with this website compendium.     

Aroma characterization of various apricot varieties using headspace–solid phase microextraction combined with gas chromatography–mass spectrometry and gas chromatography–olfactometry

The characterization of the aromatic profile of several apricot cultivars with molecular tracers in order to obtain objective data concerning the aromatic quality of this fruit was undertaken using headspace–solid phase microextraction (HS–SPME). Six apricot cultivars were selected according to their organoleptic characteristics: Iranien, Orangered, Goldrich, Hargrand, Rouge du Roussillon and A4025. The aromatic intensity of these varieties measured by HS–SPME–Olfactometry were defined and classified according to the presence and the intensity of grassy, fruity and apricot like notes. In the six varieties, 23 common volatile compounds were identified by HS–SPME–GC–MS. Finally, 10 compounds, ethyl acetate, hexyl acetate, limonene, β-cyclocitral, γ-decalactone, 6-methyl-5-hepten-2-one, linalool, β-ionone, menthone and (E)-hexen-2-al were recognized by HS–SPME–GC–O as responsible of the aromatic notes involved in apricot aroma and considered as molecular tracers of apricot aromatic quality which could be utilized to discriminate apricot varieties.     

Tests of potential functional barriers for laminated multilayer food packages. Part I: Low molecular weight permeants

The advent of the functional barrier concept in food packaging has brought with it a requirement for fast tests of permeation through potential barrier materials. In such tests it would be convenient for both foodstuffs and materials below the functional barrier (sub-barrier materials) to be represented by standard simulants. By means of inverse gas chromatography, liquid paraffin spiked with appropriate permeants was considered as a potential simulant of sub-barrier materials based on polypropylene (PP) or similar polyolefins. Experiments were performed to characterize the kinetics of the permeation of low molecular weight model permeants (octene, toluene and isopropanol) from liquid paraffin, through a surrogate potential functional barrier (25 μm-thick oriented PP) into the food simulants olive oil and 3% (w/v) acetic acid. These permeation results were interpreted in terms of three permeation kinetic models regarding the solubility of a particular model permeant in the post-barrier medium (i.e. the food simulant). The results obtained justify the development and evaluation of liquid sub-barrier simulants that would allow flexible yet rigorous testing of new laminated multilayer packaging materials.     

Chlorohydrins of bisphenol A diglycidyl ether (BADGE) and of bisphenol F diglycidyl ether (BFDGE) in canned foods and ready-to-drink coffees from the Japanese market

BADGE.2HCl and BFDGE.2HCl were determined in 28 samples of ready-to-drink canned coffee and 18 samples of canned vegetables (10 corn, 5 tomatoes and 3 others), all from the Japanese market. HPLC was used as the principal analytical method and GCMS for confirmation of relevant LC fractions. BADGE.2HCl was found to be present in one canned coffee and five samples of corn, BFDGE.2HCl in four samples of canned tomatoes and in one canned corn. No sample was found which exceeded the 1mg/kg limit of the EU for the BADGE chlorohydrins. However the highest concentration was found for the sum of BFDGE.2HCl and BFDGE.HCl.H₂O at a level of 1.5mg/kg. A Beilstein test confirmed that all cans containing foods contaminated with BADGE.2HCl or BFDGE.2HCl had at lest one part coated with a PVC organosol.     

European priorities for research to support legislation in the area of food contact materials and articles

A strong science base is required to underpin the planning and decision-making process involved in determining future European community legislation on materials and articles in contact with food. Significant progress has been made in the past 5 years in European funded work in this area, with many developments contributing to a much better understanding of the migration process, and better and simpler approaches to food control. In this paper this progress is reviewed against previously identified work-areas (identified in 1994) and conclusions are reached about future requirements for R&D to support legislation on food contact materials and articles over the next 5 or so years.     

Analysis of benzo[a]pyrene in spiked fatty foods by second derivative synchronous spectrofluorimetry after microwave-assisted treatment of samples

A simple, rapid and inexpensive method has been developed for the determination of benzo[a     

The development of reference materials for paralytic shellfish poisoning toxins in lyophilized mussel. II: Certification study

This paper describes the second part of a project undertaken to develop certified mussel reference materials for paralytic shellfish poisoning toxins. In the first part two interlaboratory studies were undertaken to investigate the performance of the analytical methodology for several PSP toxins, in particular saxitoxin and decarbamoyl-saxitoxin in lyophilized mussels, and to set criteria for the acceptance of results to be applied during the certification exercise. Fifteen laboratories participated in this certification study and were asked to measure saxitoxin and decarbamoyl-saxitoxin in rehydrated lyophilized mussel material and in a saxitoxin-enriched mussel material. The participants were allowed to use a method of their choice but with an extraction procedure to be strictly followed. The study included extra experiments to verify the detection limits for both saxitoxin and decarbamoyl-saxitoxin. Most participants (13 of 15) were able to meet all the criteria set for the certification study. Results for saxitoxin.2HCl yielded a certified mass fraction of <0.07 mg/kg in the rehydrated lyophilized mussels. Results obtained for decarbamoyl-saxitoxin.2HCl yielded a certified mass fraction of 1.59+/-0.20 mg/kg. The results for saxitoxin.2HCl in enriched blank mussel yielded a certified mass fraction of 0.48 +/- 0.06 mg/kg. These certified reference materials for paralytic shellfish poisoning toxins in lyophilized mussel material are the first available for laboratories to test their method for accuracy and performance.     

Announcing a new international peer-reviewed journal:Food Science and Human Wellness now accepting manuscript submission in English

<正>We are pleased to announce the launch of a new international peer-reviewed journal-Food Science and Human Wellness,ISSN 2213-4530,which is an open access journal,produced and hosted by Elsevier B.V.on behalf of Beijing Academy of Food Sciences.Food Science and Human Wellness is an international peer-reviewed English journal that provides a forum for the dissemination of the