内外双环流气升式发酵罐的开发

基于对实际应用中气升式循环发酵罐的分析研究，提出了一种新的动力输入方式，并由此开发了内外双环流气升式发酵罐，在１３０ｍ＾３发酵罐中进行谷氨酸发酵试验表明：无菌压缩空气耗量比一般气升式发酵罐减少５０％，与通气搅拌式发酵罐相比空气耗量减少１２％，总能量消耗分别下降２３     

300L气升式反应器高密度培养生产PHB实验

在３００Ｌ气升式反应器中，用欧文氏工程菌Ｅｃｃ１３Ｂ－１，以工业蔗糖为碳源，采用流加补料方式，进行高密度培养生产ＰＨＢ实验培养时间２５ｈ，最大干细胞浓度达４３９５ｇ／Ｌ，细胞ＰＨＢ含量５００２％实验证明气升式反应器适合于ＰＨＢ工程菌的高密度培养     

衣康酸发酵及动力学模型

研究了土曲霉在１０Ｌ搅拌罐和２００Ｌ气升罐中分批发酵生产衣康酸的过程．搅拌罐和气升罐中衣康酸最终浓度分别达到８０．１和８５．１ｇ／Ｌ，对初糖的转化率分别为５０％和５６．７％．同时，对衣康酸发酵建立了一个包括菌体生长、底物消耗、产物形成的动力学模型，根据实验数据，对动力学参数进行了优化，并且对衣康酸合成的动力学机制进行了讨论．     

分段式内循环气升反应器的实验研究

８００Ｌ分段式内循环气升反应器冷模实验结果表明：该型式的反应器具有良好的混合与传质性能。应用于Ｌ－苹果酸发酵，菌体收率、发酵周期等指标均优于同等规模的机械搅拌罐。     

气升式内环流反应器在红霉素发酵中应用初探

针对需要流加补料的发酵过程 ,设计出分段导流筒气升式内环流反应器。对在该反应器内进行的红霉素发酵过程进行了实验研究 ,从不同角度考察了该反应器用于红霉素发酵生产过程的可能性。     

驱油微生物DSGT的发酵及现场应用

以气升式发酵罐代替传统的动态搅拌装置对驱油微生物DSGT进行了发酵工艺条件和发酵参数的研究,通过单因素试验和正交试验得出,当循环泵流量90m3/h、喷雾喷头数量65个、发酵温度65℃、培养基量2%、发酵时间60h、营养因子配比3时,菌浓可达到4.8×108个/ml,且气升式反应器的发酵效果明显好于传统搅拌式反应釜。高效生物活性剂能提高菌种繁殖速度和数量,添加量0.5‰时菌浓可提高到7.8×108个/ml。现场试验表明,注入微生物菌液前后,采出井日产液由41t上升到54.6t,增幅33.1%;日产油由8.9t上升到19.1t,增幅114%;含水由78.2%降到65%;平均每天单井增油0.7t,投入产出比为1:1.99。     

一种改进型外环流气升式反应器及其应用的初步研究

一种改进型外环流气升式反应器,将其应用于从酒精到醋酸一步发酵。通过与普通气升式反应器酒精发酵结果的比较,证明了该改进型气升式反应器在厌氧发酵领域推广应用的可能性。并在此基础上提出进一步改进展望,使其可应用于酒精发酵分离耦合的研究以及作为光生物反应器进行藻类的培养。     

气升式反应器在发酵工业中的应用研究进展

气升式反应器是一种用于气液两相或气液固三相过程的接触式反应装置,简要综述了气升式反应器在发酵工业中的应用研究进展,按照集成强化的思路开发的气升式膜生物反应器被重点介绍。     

环流反应器中固定化酵母乙醇连续发酵研究

制备了一种重化固定化酵母颗粒，可有效防止发酵过程中固定化颗粒上浮，用于气升式内环流反应器，可与器内流体形成良好循环。同时在气升式内环流反应器内对固定化酵母乙醇连续发酵过程进行了研究，得到发酵最优控制条件。通过与其它发酵器对比发现，内环流反应器用于固定化酵母乙醇发酵过程，可大大提高乙醇产率。     

5000升发酵罐半连续发酵生产工艺工业性试验

本文介绍了利用樟树农业学校专利所提供的试验条件,利用鹤岗当地的原料在广东进行5000升发酵罐半连续发酵生产 HA—M 蛋白饲料的情况及试验结果。证明半连续发酵生产工艺是可行的。     

氧化亚铁硫杆菌在气升式反应器中培养条件对其生长特性的影响

构建了一种用于菌体培养-矿物浸取的气升式反应器. 分别采用Leathen和9K培养基研究了不同条件下氧化亚铁硫杆菌在气升式反应器中的生长、Fe2+氧化速率、溶氧随培养时间的变化及其相互关系. 通过测定溶氧和Fe2+氧化速率可了解培养条件对微生物生长特性的影响.     

Production of berberine in immobilized plant cell culture ofThalictrum rugosum

Thalictrum rugosum cells were immobilized in calcium alginate, where they continued to live with their biological activity. The immobilized living cells performed the production of berberine in both shake flasks and an airlift bioreactor. Berberine formation was growth associative and most of the berberine produced was stored intracellularly. Rapid hydrolysis of sucrose and preference of glucose over fructose during the growth stage was observed. Phosphate-deficient media increased berberine production and prevented the dissolution of alginate beads. The behavior of immobilized cells grown in an airlift reactor was compared with that of the corresponding shake flask culture with respect to growth and berberine production. The rate of cell growth and berberine production in an airlift reactor operation was higher than those in packedcolumn reactor operation due to a better oxygen transfer.     

Modeling of Mass Transfer in an Internal Loop Airlift Reactor

A complete mass transfer model was established for an internal loop airlift reactor. All sections of the reactor, including bottom, riser, top, and downcomer, were taken into account. A numerical method was developed to solve the mass transfer model. The effectiveness of the model was validated by experimental measurements. Based on numerical results of the model, the dynamical mass transfer process in the reactor was analyzed in detail. Some mass transfer characteristics of the airlift reactor were revealed. The results indicated that the proposed model well predicted the mass transfer in the internal loop airlift reactor.     

A mathematical model for unsteady-state oxygen transfer in an external-loop airlift reactor

A mathematical model for simulation of unsteady-state oxygen transfer in an external-loop airlift reactor was developed. The airlift reactor was represented by a number of interconnected tanks, each of which was assumed to be well mixed. The effect of gas circulation rate on the oxygen transfer was considered. The model can be used for the determination of mass transfer performance of the airlift reactor in which the whole liquid phase cannot be assumed to be fully backmixed. The simulation results showed a good agreement with experimental results.     

AILR+MBR工艺处理焦化废水虚拟设备

采集三相气提升循环流化床(AILR) 膜生物反应器(MBR)工艺处理焦化废水的中试试验数据,在Labview的编程平台上,基于MBR脱C、脱N模型、溶解氧浓度和膜面流速计算模型,对该工艺进行了动态模拟,模拟结果表明:出水COD、NH3-N浓度等模拟值能够很好地和实测值相吻合,相对误差小。     

MULTIPHASE HYDRODYNAMICS AND SOLID-LIQUID MASS TRANSPORT IN AN EXTERNAL-LOOP AIRLIFT REACTOR—A COMPARATIVE STUDY

The solid-solid mass transfer performance of an external-loop airlift reactor was measured by dissolution of benzoic acid coated on nylon-6 particles, and the hydrodynamics of the gas-liquid-solid multiphase system in the airlift reactor were investigated. The solid-liquid system was designed to simulate the micro-carrier culture of animal cells, and some typical suspensions of immobilized enzyme particles.

The solid-liquid mass transfer coefficient remained constant below a superficial air velocity of 0.04 ms^-1 for the particles examined, but increased rapidly with further increase in gas velocity. Solids loading (0.3-3.5% w/w) did not affect the mass transfer coefficient in turbulent flow.

The mass transfer coefficient was correlated with energy dissipation rate in the airlift reactor. The mass transfer coefficient in stirred vessels, bubble columns, fluidized beds, and airlift reactors was compared.

Over an energy dissipation Reynolds number of 4-400, the solid-liquid mass transfer coefficient in the airlift device was comparable to that obtainable in fluidized beds. The performance of the airlift was distinctly superior to that of bubble columns and stirred tanks.     

气升式环流反应器研究与应用进展

综述了气升式反应器性能的主要影响因素研究及其最新的应用，提出今后研究工作的主攻方向，并介绍了最近超声气升式反应器及光气升式反应器的研究结果。     

气固环流反应器的研究进展

气固环流反应器是将气液环流理论与稠密气固流态化理论进行合理移植、耦合而形成的一种新型气固流化床反应器。对近年来国内外学者在该领域的研究进行了回顾,参考了内循环流化床的一些研究结果,对操作条件、几何结构对气固环流反应器内两相流体力学行为的影响规律进行了总结和分析,并对进一步的研究进行了展望。     

大颗粒三相环隙气升式环流反应器流体力学行为

研究了大颗粒体系气升式环流反应器的流体力学行为,考察了表观气速和颗粒质量分数对床层膨胀高度、循环液速和固含率分布的影响。实验结果表明,按颗粒的运动状态不同可以将反应器内的流动分为3个区域,即固定床区域、膨胀床区域和循环床区域,各流动区域内的流动行为存在显著差异。随着颗粒质量浓度的增大,起始流化气速和最小循环气速均显著增大。基于三相流化床的流化模型和环流反应器的特点建立了相应的数学模型,对大颗粒三相气升式环流反应器的起始流化气速和最小循环气速进行了预测,模型预测值与实验测量值吻合良好。