CO_2激光照射对表皮LC的影响

郎格罕氏细胞(Lange Hans cell.LC)是从骨髓中的前体细胞衍化而来的树突状细胞,主要位于表皮基层上方.有摄取抗原、处理抗原和传递抗原的功能,起着免疫系统外围前哨的作用.LC的功能可受各种理化因子的影响,是否也会受到激光照射的影响?鲜见文献报道.本文探讨低功率CO2激光照射后,表皮LC的数目形态和分布的情况.     

紫外线照射损伤表皮郎格罕细胞的光、电镜研究

紫外线照射能减少表皮内郎格罕细胞(LC)的数目,并引起其形态结构改变。为了进一步探讨紫外线照射对LC影响的作用机理,本研究利用ATPase染色法显示游离表皮内LC,于光镜下观察不同剂量的紫外线照射豚鼠皮肤后,表皮LC密度动态变化,同时采用组化电镜手段进行光、电镜对比研究照射前后LC的形态结构。实验用Hartley杂交豚鼠30只,分6组,分别接受不等剂量的紫外线照射。照射前脱去背部毛,每日照射1次,连续4天,6组动物接受照射的总剂量     

激光血管内照射治疗银屑病的研究

22 5例银屑病患者 ,经用激光血管内照射 ,总有效率 10 0 % ,疗效随照射次数的增加而递增。激光血管内照射能改善微循环、调整机体的细胞免疫功能、调整表皮细胞的代谢     

He-Ne激光照射对小鼠细胞免疫功能的影响

本文采用He—Ne激光照射小鼠胸腺区及腹部。观察激光照射对T细胞及巨噬细胞的影响,结果激光照射组小鼠淋转率及吞噬率较对照组明显增高,两组间相比 P<0.01,证明激光具有增强细胞免疫功能的作用。     

铜蒸气激光照射对血管平滑肌细胞凋亡的诱导作用及对其增殖的影响

目的：研究510.6nm铜蒸气激光照射对体外培养的血管平滑肌细胞（VSMC）凋亡的诱导作用，以及对增殖细胞核抗原（PCNA）表达的影响，探讨铜蒸气激光照射在经皮冠状动脉成形术（PTCA）后再狭窄（RS）的防治作用。方法：贴块法培养兔VSMC，510.6nm铜蒸气激光照射后透射电镜观察凋亡细胞形态学改变，TUNEL法计数凋亡细胞，免疫组化染色法计数照光对PCNA阳性表达率的影响。结果：激光照射后，VSMC凋亡率较未照光组增加12.8倍，而PCNA表达阳性率降低27．9％倍；电镜下观察细胞呈典型的凋亡形态学改变。结论：铜蒸气激光照射可以诱导VSMC凋亡，而且抑制其增殖，在RS的防治中具有一定的作用。     

氦-氖激光体外照射正常人体淋巴细胞转化试验的初步观察

通过小功率He-Ne激光直接照射试管内正常人体淋巴细胞，观察其转化情况，以了解激光照射对人体细胞免疫功能有无影响。     

He-Ne激光对白细胞中脱氢酶影响的细胞化学观察

用He—Ne激光辐照小鼠胸骨,采用细胞化学方法观察激光照射前后PMN和LC内LDH、SDH和GDH活性的变化。结果表明适量的He—Ne激光辐照能激活白细胞,促进三种脱氢酶活性增强,提高三羧酸循环和糖酵解代谢。     

He-Ne激光体外照射人体淋巴细胞的转化情况

为了了解He-Ne激光照射对人体细胞免疫功能的影响，通过小功率He-Ne激光直接照射试管内正常人的淋巴细胞，对其转化情况进行了观察分析。     

激光照射对荷瘤小鼠淋巴细胞活性的影响

采用MTT还原分析法研究氦氖激光照射对SP2 /O骨髓瘤小鼠脾细胞、胸腺细胞的增殖活性的影响。结果表明激光具有增强荷瘤小鼠免疫细胞活性的功能。激光组与对照组相比P <0 0 1,差异明显。由此证明氦氖激光照射免疫器官区 (脾区 )具有活化淋巴细胞 ,促进淋巴细胞DNA合成 ,提高机体免疫力的作用。     

Nd:YVO4激光对蚕豆细胞生物学效应的研究

本试验采用Nd YVO4 激光照射蚕豆已萌发的种子。Nd YVO4 激光照射的时间分别为5min .10min .15min。对照射后蚕豆根尖下细胞中的染色体损伤情况进行了研究。经Nd YVO4 激光照射后,蚕豆根尖细胞中的染色体的畸变类型有染色体桥,断片,落后染色体,微核。经Nd YVO4 激光照射后,蚕豆根尖细胞中有丝分裂指数随着照射的时间的延长而降低。试验结果表明,Nd YVO4 激光对蚕豆根尖细胞染色体和有丝分裂有明显影响。     

Comparison of the effect of a proteolytic enzyme dispase and a chelator for the preparation of epidermal sheet from nude mice.

Histochemical studies of epidermal Langerhans cells require well-preserved epidermal sheets. We studied the conditions for the preparation of the epidermal sheets from the skin of the ear, hind limb and trunk of nude mice. Two types of commercial dispase, and ethylenediaminetetraacetic acid (EDTA), were used at several concentrations and the effects were compared. Dermo-epidermal separation was evaluated by the preservation of ATPase activity of Langerhans cells in the epidermal sheet and by the ultrastructure of epidermal cells (Langerhans cells and keratinocytes) as well as epidermal-dermal junction. Good separation depended on the combination of the concentration of the reagent and site of the skin. The concentrations of both dispase and EDTA effective for separation of the epidermis in the nude mouse were lower than those in other mouse strains reported.     

He-Ne激光对家兔淋巴结影响的光镜和电镜观察

以Ｈｅ－Ｎｅ激光局部照射家兔颈前部及穴位照射，取颈前淋巴结进行光镜和电镜观察，同时用单向免疫扩散法测血清ＩｇＧ含量。结果表明，局部照射和穴位照射均能使淋巴结内多种细胞的超微结构发生改变，细胞功能表现活跃。穴位照射组血中ＩｇＧ含量较对照组明显升高。说明Ｈｅ－Ｎｅ激光照射增强机体细胞免疫和体液免疫机能是有细胞学基础的。提示穴位照射能增强全身免疫机能，而局部照射只能增强被照部位的免疫机能     

An ultrastructural study on the effect of streptozotocin on the islets of Langerhans in mice

The ultrastructural changes in the morphology of the islets of Langerhans in response to streptozotocin were studied in the mice pancreas. Male white albino CSI mice were given a single intravenous injection of 75 mg kg(-1) body weight streptozotocin, and were sacrificed at different time intervals up to 48 h following the treatment. Their pancreases were excised and randomly processed for electron microscopic examination. Hyperglycaemia and glucosuria were detected 8 h after treatment, became remarkably high at 24 h and persisted then after. Light and electron microscopic examination of the islets of Langerhans from treated mice revealed an early chromatin aggregation and cytoplasmic vesiculation in the central B cells during the first 2 h of treatment. Nuclear shrinkage and pyknosis with swelling of mitochondria and endoplasmic reticulum were evident 8 h later, and lysis of B cells occurred 12 h after treatment. The morphology of A and D cells at the margin of the islets and in between B cell debris looked perfectly unaltered. Macrophage infiltration among lytic B cells was seen 24 h after drug administration, which contained clear and large phagocytic vacuoles. The necrobiotic and phagocytic figures disappeared from the pancreatic sections of 48 h treated mice, and the islets were smaller in size and consisted entirely of intact A and D cells with occasional degranulated B cells. No features of apoptosis were ever recorded, and the exocrine pancreatic tissue was protected from the effect of streptozotocin. In conclusion, the present study illustrates the sequence of morphological changes that occurs in the islets of Langerhans of mice after streptozotocin administration. It also confirms that streptozotocin at a high single dose in mice produces a specific necrosis of B cells with no evidence of apoptotic figures as another mechanism of cell death.     

He-Ne激光照射对胃癌SGC7901细胞生长的影响研究

目的：探讨He—Ne激光照射对胃癌SGC7901细胞生长的影响。方法：将肿瘤细胞用含10％牛血清RPMI1640培养液培养,并用波长为632．8nm和功率密度为78mw／cm^2的He-Ne激光分别以23J／cm^2,46J／cm^2,72J／cm^2,140J／cm^2,280J／cm^2能量密度照射肿瘤细胞1次或3次,然后用激光共聚焦显微镜检测细胞形态等和四氮唑盐法（MTT）进行活细胞记数。结果：激光照射对肿瘤细胞的形态没有明显影响。这当照射1次时,各种能量密度的激光照射都促进肿瘤细胞的生长。当照射3次时。能量密度小于72J／cm^2时激光照射促进肿瘤细胞的生长,而当能量密度大干72J／cm^2时,反而抑制肿瘤细胞的生长。不管照射1次还是3次23J／cm^2能量密度的激光照射对肿瘤细胞生长的促进作用最明显。结论：He—Ne激光照射对肿瘤细胞生长起促进作用还是抑制作用与激光的累积照射剂量有关。     

630nm半导体激光照射对小鼠S180腹水瘤细胞的影响

目的探讨 6 30nm半导体激光体外照射对S180腹水瘤肿瘤细胞的影响方法用输出功率 2W、不同功率密度 (10 0、2 0 0、2 5 0mW/cm2 )不同的照光时间 (2 0、15、10、5、3min)的半导体激光照射S180腹水瘤肿瘤细胞 ,照光后继续培养 2 4小时。观察照光前后不同时间段的细胞形态改变。用流式细胞技术测定照光前后S180腹水瘤肿瘤细胞的凋亡情况。结果 :1.实验组与对照组细胞在照光前后各时间段 (照光前、后即刻、培养 2 4小时 )的细胞形态相比较无明显改变。 2 .实验组与对照组细胞经流式细胞仪检测细胞凋亡情况无明显差异。结论 :单用光动力学治疗剂量的 6 30nm半导体激光对体外肿瘤细胞无明显杀伤作用 ,且不诱导S180腹水瘤肿瘤细胞发生凋亡。     

GaN基紫外探测器的电子辐照效应

用能量为0.8 MeV的电子对非故意掺杂的GaN材料进行了辐照,光致发光谱（PL谱）表明,辐照使PL谱的强度随电子注量依次降低,且主发光峰蓝移,在注量较高时,在3.36 eV附近,出现新的发光峰。制备了SiN/GaN的MIS结构,并对其进行电子辐照,通过测量C-V曲线计算得到SiN/GaN之间的界面态随着电子辐照注量的增加而增加。制备了GaN基p-i-n 结构可见盲正照射紫外探测器并进行电子辐照,测量了辐照前后器件的I-V曲线和光谱响应曲线。实验表明,小注量的电子辐照对器件的反向暗电流影响不大,当电子注量≥5×10¹⁶ n/cm²时才使器件的暗电流增大一个数量级。辐照前后器件的光谱响应曲线表明,电子辐照对器件的响应率没有产生明显的影响。利用GaN材料和MIS结构的辐照效应分析了器件的辐照失效机理。     

三氧化二砷对照射后人肺癌细胞MGMT和VEGF表达的影响

目的：本文探讨三氧化二砷（Arsenic trioxide,As2O3）对受照后人肺癌A2细胞株MGMT和VEGF表达的影响。方法：设立空白对照组、照射组（直线加速器X射线照射,2Gy）、As2O3组（1.0μmol/L As2O3）、As2O3＋照射组（1.0μmol/L As2O3＋X射线照射,2Gy）。平板克隆形成试验测定各实验组细胞集落形成率,用RT-PCR和免疫组化检测MGMT（甲基鸟嘌呤甲基转移酶）、VEGF（血管内皮生长因子）基因和蛋白水平的变化。结果：As2O3＋照射组细胞集落形成率较As2O3组及照射组明显下降,与As2O3组或照射组比较差异具有显著性（P〈0.01）。As2O3＋照射组细胞MGMT、VEGF基因表达明显减少,而且MGMT、VEGF蛋白的表达明显降低,与As2O3组或照射组比较差异具有显著性（P〈0.01）。结论：As2O3能使受照后人肺癌A2细胞MGMT、VEGF基因及蛋白表达水平明显下调,可能是As2O3对人肺癌细胞辐射增敏的机制之一。     

Study of the relaxation of the excess current in silicon Schottky diodes

This study is devoted to investigation of the relaxation of excess current in silicon α-NiTi-n-Si Schottky diodes subjected to either γ-ray radiation or local disturbance of the interface structure using a diamond indenter. A decrease in the excess diode current is attained using both thermal annealing and ultra-sound irradiation. Simultaneously, the parameters of solar cells manufactured from the above-mentioned Schottky diodes subjected to irradiation with γ-ray photons and to single or double irradiation with ultra-sound are studied. It is shown that, after the effect of the diamond indenter, the excess current decreases as a result of thermal annealing; however, a decrease in the excess current to the initial value is not attained. The photoelectric parameters of the studied solar cells before irradiation and after irradiation with γ-ray photons and after single or double irradiation with ultrasound show that ultrasonic treatment is more efficient than thermal annealing.     

连续激光对太阳能电池辐照效应的波段性研究

为了研究太阳能电池在连续激光辐照下的波段性效应,使用位于波段内外的3种波长连续激光对单晶硅太阳能电池的辐照效应和损伤效应进行了实验研究,采用观察电池对激光的响应,对比电池的损伤形貌和太阳能电池组成材料的吸收曲线差异的方法,得到了太阳能电池对本征吸收长波限附近波长激光的吸收机制,分析了10.6μm激光和1064nm激光对...     

氦-氖激光处理蕃茄种子最适剂量的研究

激光作为育种手段已被应用了多年并且产生了许多良好的效果。但在这个领域中仍有许多具体问题有待于探索,如对某种作物或品种应用何种激光、什么剂量使其向人们预期的方向发展等等。我们以氦-氖激光为光源对蕃茄的两个品种的种子进行了不同功率密度、不同时间