共查询到20条相似文献,搜索用时 15 毫秒
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Gui‐tang Chen Lin Zhao Li‐yan Zhao Tao Cong Shan‐fen Bao 《Journal of the science of food and agriculture》2007,87(2):357-362
Peanut protein was hydrolysed with a commercial protease, Alcalase 2.4L, and the resulting hydrolysate was investigated for its antioxidant activities, including the ability to inhibit the autoxidation of linoleic acid, the scavenging effect on the 1,1‐diphenyl‐2‐picrylhydrazyl (DPPH) free radical, the reducing power and the inhibition of liver lipid oxidation. As compared with the peanut protein, peanut protein hydrolysate showed strong inhibition of the autoxidation of linoleic acid, to scavenge DPPH free radical and showed strong reducing power. Moreover, peanut protein hydrolysate also displayed noticeable inhibition of liver lipid autoxidation and lipid oxidation induced by H2O2 or Fe2+ in vitro. All these effects of the sample were concentration‐dependent. These results suggest that peanut protein hydrolysate could be a suitable natural antioxidant and may be a health food for humans. Copyright © 2006 Society of Chemical Industry 相似文献
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Effects of ultrasound treatment on physico‐chemical,functional properties and antioxidant activity of whey protein isolate in the presence of calcium lactate
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Zhanmei Jiang Kun Yao Xiangying Yuan Zhishen Mu Zengli Gao Juncai Hou Lianzhou Jiang 《Journal of the science of food and agriculture》2018,98(4):1522-1529
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Comparative study on antioxidative activity of yellow stripe trevally protein hydrolysate produced from Alcalase and Flavourzyme 总被引:3,自引:0,他引:3
Vilailak Klompong Soottawat Benjakul Duangporn Kantachote Kirby Dale Hayes & Fereidoon Shahidi 《International Journal of Food Science & Technology》2008,43(6):1019-1026
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Qian Liu Jing Li Baohua Kong Peijun Li Xiufang Xia 《International Journal of Dairy Technology》2014,67(2):220-228
This study was conducted to investigate the physicochemical and antioxidant properties of Maillard reaction products (MRP) prepared by heating whey protein isolate (WPI) and reducing sugars (glucose and galactose) to 95 °C for different lengths of time (0–6 h). The results revealed that the colour, intermediate products, browning intensity and the antioxidant activities of the MRP increased as the reaction time increased (P < 0.05), whereas the pH value and free amino group content decreased (P < 0.05). Sodium dodecyl sulphate–polyacrylamide gel electrophoresis indicated that the covalently linked conjugates of WPI and sugars were formed. The results indicate that the Maillard reaction could improve the antioxidant capacity of WPI. 相似文献
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Laurent Picot Rozenn Ravallec Martine Fouchereau‐Péron Laurent Vandanjon Pascal Jaouen Maryse Chaplain‐Derouiniot Fabienne Guérard Aurélie Chabeaud Yves LeGal Oscar Martinez Alvarez Jean‐Pascal Bergé Jean‐Marie Piot Irineu Batista Carla Pires Gudjon Thorkelsson Charles Delannoy Greta Jakobsen Inez Johansson Patrick Bourseau 《Journal of the science of food and agriculture》2010,90(11):1819-1826
BACKGROUND: Numerous studies have demonstrated that in vitro controlled enzymatic hydrolysis of fish and shellfish proteins leads to bioactive peptides. Ultrafiltration (UF) and/or nanofiltration (NF) can be used to refine hydrolysates and also to fractionate them in order to obtain a peptide population enriched in selected sizes. This study was designed to highlight the impact of controlled UF and NF on the stability of biological activities of an industrial fish protein hydrolysate (FPH) and to understand whether fractionation could improve its content in bioactive peptides. RESULTS: The starting fish protein hydrolysate exhibited a balanced amino acid composition, a reproducible molecular weight (MW) profile, and a low sodium chloride content, allowing the study of its biological activity. Successive fractionation on UF and NF membranes allowed concentration of peptides of selected sizes, without, however, carrying out sharp separations, some MW classes being found in several fractions. Peptides containing Pro, Hyp, Asp and Glu were concentrated in the UF and NF retentates compared to the unfractionated hydrolysate and UF permeate, respectively. Gastrin/cholecystokinin‐like peptides were present in the starting FPH, UF and NF fractions, but fractionation did not increase their concentration. In contrast, quantification of calcitonin gene‐related peptide (CGRP)‐like peptides demonstrated an increase in CGRP‐like activities in the UF permeate, relative to the starting FPH. The starting hydrolysate also showed a potent antioxidant and radical scavenging activity, and a moderate angiotensin‐converting enzyme (ACE)‐1 inhibitory activity, which were not increased by UF and NF fractionation. CONCLUSION: Fractionation of an FPH using membrane separation, with a molecular weight cut‐off adapted to the peptide composition, may provide an effective means to concentrate CGRP‐like peptides and peptides enriched in selected amino acids. The peptide size distribution observed after UF and NF fractionation demonstrates that it is misleading to characterize the fractions obtained by membrane filtration according to the MW cut‐off of the membrane only, as is currently done in the literature. Copyright © 2010 Society of Chemical Industry 相似文献
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Inhibitory properties of bambara groundnut protein hydrolysate and peptide fractions against angiotensin‐converting enzymes,renin and free radicals
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Abimbola K Arise Adeola M Alashi Ifeanyi D Nwachukwu Sunday A Malomo Rotimi E Aluko Eric O Amonsou 《Journal of the science of food and agriculture》2017,97(9):2834-2841
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Influence of degree of hydrolysis on functional properties,antioxidant activity and ACE inhibitory activity of peanut protein hydrolysate 总被引:8,自引:0,他引:8
Functional properties, antioxidant and Angiotensin-converting enzyme (ACE) inhibitory activities of peanut protein isolate (PPI) and peanut protein hydrolysate (PPH) prepared using Alcalase, at different (10%, 20%, 30% and 40%) degrees of hydrolysis, (DH) were investigated. Hydrolysis (at DH > 10%) significantly (p < 0.05) improved the solubility (>80%) of PPI, especially in the pH range of 4–6. However, PPI showed better emulsifying and foaming properties than PPH (p < 0.05). As DH increased, ferrous ion chelating activity, 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical-scavenging activity and ACE inhibitory activity of PPH increased, while reducing power decreased (p < 0.05). Bleaching of beta-carotene by linoleic acid was suppressed better by PPI and PPH at 10% DH than of PPH at higher DH. Thus, the results reveal that DH affects functional properties, antioxidant and ACE inhibitory activities of peanut protein. 相似文献
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Carla Pires Teresa Clemente Irineu Batista 《Journal of the science of food and agriculture》2013,93(4):771-780
BACKGROUND: The production of fish protein hydrolysates (FPH) is a convenient technology for upgrading fish by‐products. The aim of this work was to study three different methods of FPH preparation from Cape hake by‐products to improve yield and quality. Functional and antioxidative properties of all FPHs were determined. RESULTS: The protein content of hake FPH was in the range 807–860 g kg?1 and the degree of hydrolysis was between 19% and 22%. The maximum yield (71.9%) was achieved by methodology B but the hydrolysate was darker. The peptide profile of all FPHs was very similar. FPH prepared by methodology C had significantly higher emulsifying activity index and hydrolysate prepared by methodology B had the highest foaming capacity. The solubility of FPH was in the range 71–76% and increased the water‐holding capacity of minced fish by about 9%. The fractionation of FPH obtained by methodologies A and B allowed concentrating peptides with higher radical scavenging activity and reducing power. CONCLUSION: The properties of the FPH prepared indicated that they can be used in food systems as natural additives, particularly to improve their water‐holding capacity. © 2012 Society of Chemical Industry 相似文献
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目的研究不同分子量分布对大豆分离蛋白水解肽乳化性及抗氧化性的影响。方法采用超滤技术将超高压协同酶解制备的大豆分离蛋白水解物分离得到5个分子量肽段,即分子量(Molecular weight, Mw)<3500 u、3500 u< Mw <7000 u、7000 u< Mw <10000 u、10000 u< Mw <20000 u和Mw >20000 u。通过测定乳化活性、乳化稳定性、电势、粒径、还原力、以及对 2,2-联苯基-1-苦基肼基(2,2-diphenyl-1-picrylhydrazyl, DPPH)和 2,2''-联氮-双(3-乙基苯并噻唑-6-磺酸)(2, 2-azinobis (3-ethylbenzothiazoline-6-sulfonic acid), ABTS)自由基清除率,分析不同分子量水解肽的乳化性及抗氧化性变化。结果7000 u< Mw <10000 u的水解肽具有较高的乳化活性、电势和较小的粒径,而10000 u< Mw <20000 u的水解肽则表现出较优的乳化稳定性,但与7000 u< Mw <10000 u的组分差异不显著(P>0.05);水解肽的抗氧化性随肽段分子量的减小呈逐渐增大趋势,且不同分子量肽段与还原力和DPPH自由基清除能力均呈极显著负相关性(P<0.01),与ABTS自由基清除能力呈显著负相关性(P<0.05)。结论 超高压协同酶法制备的大豆分离蛋白水解肽,乳化性和抗氧化性与其分子量分布有密切关联,这为相关功能性产品的开发提供了较为切实的理论依据。 相似文献
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Identification of angiotensin converting enzyme inhibitory and antioxidant peptides in a whey protein concentrate hydrolysate produced at semi‐pilot scale
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Martina B. O'Keeffe Celia Conesa Richard J. FitzGerald 《International Journal of Food Science & Technology》2017,52(8):1751-1759
Antioxidant and angiotensin converting enzyme (ACE) inhibitory peptides were identified in a 5 kDa ultrafiltration permeate of a whey protein hydrolysate generated at semi‐pilot scale. Further laboratory scale ultrafiltration of this 5 kDa permeate resulted in a 0.65 kDa permeate with antioxidant, (1.11 ± 0.074 μmol TE per mg dry weight, oxygen radical absorbance capacity, ORAC) and ACE inhibitory (ACE IC50 0.215 ± 0.043 mg mL?1) activities. Semi‐preparative (SP) reverse phase high‐performance liquid chromatography (RP‐HPLC) of the 0.65 kDa permeate resulted in a fraction (SP_F3) with a 4.4‐fold increase in ORAC activity (4.83 ± 0.45 μmol TE mg dry weight) and a 1.3‐fold increase in ACE inhibitory activity (84.35 ± 1.36% inhibition when assayed at 0.28 mg mL?1). Peptides within SP_F3 were identified using UPLC‐ESI‐MS/MS. Met‐Pro‐Ile had the highest ORAC activity (205.75 ± 12.08 μmol TE per mmol peptide) while Met‐Ala‐Ala and Val‐Ala‐Gly‐Thr had the highest ACE inhibitory activities (IC50:515.50 ± 1.11 and 610.30 ± 2.41 μm , respectively). 相似文献
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The ability of in vitro antioxidant assays to predict the efficiency of a cod protein hydrolysate and brown seaweed extract to prevent oxidation in marine food model systems
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Rósa Jónsdóttir Margrét Geirsdóttir Patricia Y Hamaguchi Polona Jamnik Hordur G Kristinsson Ingrid Undeland 《Journal of the science of food and agriculture》2016,96(6):2125-2135
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The effects of salt concentration and pH upon protein extractability and water-binding by homogenates of turkey breast, thigh and drumstick meat, and also the effect of salt concentration and centrifugal force upon water-binding of raw meat and water-holding of cooked meat from breast and thigh were studied. Concentrations of sodium chloride above 0.3 M caused swelling of meat homogenates. With the pH value adjusted to 6.0 and with sodium chloride concentrations greater than 0.6 M, breast meat was able to swell more than leg meat. Water-binding increased with increasing pH value. Breast meat homogenates had more extractable protein than leg meat homogenates and protein extraction was increased by increasing both salt concentration and pH. Cooked breast meat retained more water than leg meat with or without salt. At low concentrations of salt, cooked breast meat retained more water than raw meats, but this was reversed at higher salt concentrations. 相似文献
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Extraction of unsaturated fatty acid‐rich oil from common carp (Cyprinus carpio) roe and production of defatted roe hydrolysates with functional,antioxidant, and antibacterial properties
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Sakhi Ghelichi Bahareh Shabanpour Parastoo Pourashouri Mona Hajfathalian Charlotte Jacobsen 《Journal of the science of food and agriculture》2018,98(4):1407-1415