Hair mercury concentrations of children and mothers in Korea: implication for exposure and evaluation

BACKGROUND: Mercury is a global pollutant that affects neurodevelopment of children. OBJECTIVE: The objectives were to measure and evaluate mercury concentration of children and mothers, and its association with exposure. METHODS: A cross-sectional assessment was done using questionnaires and hair mercury were analysed by Cold Vapor Atomic Absorption Spectrometry in the National Institute for Minamata Disease in Japan. RESULTS: A total of 112 children and 111 mothers were included; mean age was 34 months and 32 years, respectively. 17.9% of children and 34.2% of mothers had concentrations greater than 1 parts per million (ppm) as reference level. Body weight at birth, feeding methods, maternal age, and maternal education level were significantly different in each group (p<.05). Mean maternal hair mercury level (0.91 ppm) was higher than children (0.74 ppm), and has a positive correlation between them (p<.05). 68.1% of children, 75% of pregnant period, 63.4% of lactating period, and 78.6% of last six months have been consuming fish. With multiple regression analysis, hair mercury levels in children aged less than 6 months had a linear relationship with body weight at birth, gestational weeks, feeding methods (breast- or bottle- feeding) and maternal educational level. While children aged over 6 months significantly differed with gender, frequency of fish servings per week, and frequency of maternal fish consumption in lactation period. And hair mercury levels had inverse linear relationship with maternal monthly income in this age group. Maternal mercury levels had linear relationship with maternal age. CONCLUSION: Mercury levels in children may be affected by their mothers due to similar dietary patterns. Further long-term large-scale and follow-up studies are needed.     

Ecosystem matters: fish consumption, mercury intake and exposure among fluvial lake fish-eaters

Many studies use the number of fish meals as an estimate of Hg intake, although fish Hg concentrations, even within the same species, can greatly vary. Furthermore, most freshwater advisories only refer to local catch, while market fish advisories only focus on market fish, although both can contribute to Hg body burden. The present study, carried out in lakeside communities from 2 ecosystems in Quebec, Canada, sought to (i) estimate Hg intake from local freshwater sources, hunted waterfowl and market fish and seafood, and (ii) examine the relations between fish consumption, estimated Hg intake and biomarkers of exposure. A total of 238 adults (18-74 years), who had consumed local catch within the past three months, responded to an extensive interview-administered fish and waterfowl frequency questionnaire. Anthropometric measures were taken and a self-administered questionnaire was used to obtain socio-demographic information. Hg intake was estimated as µg Hg/kg body weight/day. Blood and hair samples were analyzed for Hg content. Results showed that persons from one ecosystem ate significantly more fish compared to those from the other (median: 52.1 g/day vs 38.9 g/day), but presented significantly lower concentrations of hair Hg (median: 448.0 ng/g vs 730.5 ng/g), blood organic Hg (median: 1.1 µg/L vs 3.4 µg/L) and inorganic Hg (median: 0.4 µg/L vs 0.8 µg/L). Median daily total Hg intake was 0.080 µg/kg bw/day for the former community and 0.141 µg/kg bw/day for the latter. Overall, 59.5% from the first ecosystem and 41.0% from the other, exceeded the US EPA RfD (0.1 µg/kg bw/day), while 13.2% and 6.0%, respectively, exceeded the Canadian tolerable daily intake (0.47 µg/kg bw/day) for adults. For the two groups, freshwater fish consumption frequency, but not total fish, was positively associated with bioindicators of Hg while estimated Hg intake from freshwater catch as well as from total fish consumption were positively related to Hg biomarkers. There was a positive relation between consumption and estimated Hg intake from freshwater fish and blood inorganic Hg. These findings indicate that the number of fish can be a poor surrogate for Hg exposure. The differences observed here for Hg intake and exposure reflect ecosystem disparities in fish diversity and Hg bioaccumulation. Studies and advisories need to consider Hg fish concentrations and fish-eating patterns in different ecosystems, as well as the contribution of market fish. The relation between fish consumption and inorganic Hg exposure, reported as well in other studies, needs to be further investigated.     

Fish consumption and bioindicators of inorganic mercury exposure

BACKGROUND: The direct and close relationship between fish consumption and blood and hair mercury (Hg) levels is well known, but the influence of fish consumption on inorganic mercury in blood (B-IHg) and in urine (U-Hg) is unclear. OBJECTIVE: Examine the relationship between fish consumption, total, inorganic and organic blood Hg levels and urinary Hg concentration. METHODS: A cross-sectional study was carried out on 171 persons from 7 riparian communities on the Tapajós River (Brazilian Amazon), with no history of inorganic Hg exposure from occupation or dental amalgams. During the rising water season in 2004, participants responded to a dietary survey, based on a seven-day recall of fish and fruit consumption frequency, and socio-demographic information was recorded. Blood and urine samples were collected. Total, organic and inorganic Hg in blood as well as U-Hg were determined by Atomic Absorption Spectrometry. RESULTS: On average, participants consumed 7.4 fish meals/week and 8.8 fruits/week. Blood total Hg averaged 38.6+/-21.7 microg/L, and the average percentage of B-IHg was 13.8%. Average organic Hg (MeHg) was 33.6+/-19.4 microg/L, B-IHg was 5.0+/-2.6 microg/L, while average U-Hg was 7.5+/-6.9 microg/L, with 19.9% of participants presenting U-Hg levels above 10 microg/L. B-IHg was highly significantly related to the number of meals of carnivorous fish, but no relation was observed with non-carnivorous fish; it was negatively related to fruit consumption, increased with age, was higher among those who were born in the Tapajós region, and varied with community. U-Hg was also significantly related to carnivorous but not non-carnivorous fish consumption, showed a tendency towards a negative relation with fruit consumption, was higher among men compared to women and higher among those born in the region. U-Hg was strongly related to I-Hg, blood methyl Hg (B-MeHg) and blood total Hg (B-THg). The Odds Ratio (OR) for U-Hg above 10 microg/L for those who ate >4 carnivorous fish meals/week was 4.00 [1.83-9.20]. CONCLUSION: This study adds further evidence to a positive relation between fish consumption and IHg in both blood and urine, which may result from absorption of IHg from fish or from demethylation of MeHg. The findings support the importance of assessing IHg exposure in fish-eating communities. Further studies should examine the potential toxicity of IHg in heavy fish consumers.     

Mercury exposure assessment in Iranian women's hair of a port town with respect to fish consumption and amalgam fillings

In this study we aimed to estimate the level of mercury in women's hair and its association with fish consumption and amalgam fillings in an Iranian southern port town (named Mahshahr). A total number of 195 hair samples were collected randomly from women aged 25-45 years free from occupational exposures to mercury. Additional information about these women's age, weight, height, number of pregnancies, level of education, number of amalgam fillings and fish consumption were also gathered. The determination of Hg level in hair samples was carried out by the LECO, AMA 254, Advanced Mercury Analyzer, according to ASTM standard No. D-6722. The average mercury level in these women's hair was 2.95 ± 2.33 µg/g (ranged from 0.07 to 24.46 µg/g). In the next stage, the study participants were divided into four groups with respect to their fish consumption and number of amalgam fillings; A) < 3 amalgam fillings, < 3 times fish consumption per month, B) < 3 amalgam fillings, > 7 times fish consumption per month, C) > 6 amalgam fillings, < 3 times fish consumption per month, and D) > 6 amalgam fillings, > 7 times fish consumption per month. Statistical analysis showed that the Hg level was significantly different in these four groups (P < 0.001). Mercury levels in this sample exceeded the USEPA-recommended 1 μg/g in 76.4% of the studied population. Finally, the multiple linear regression results revealed that frequency of fish consumption and amalgam restorations are the most important routes of Hg exposure. However, the main determinant of Hg exposure was the intake of Hg through fish consumption.     

Hair can be a good biomarker of occupational exposure to mercury vapor: simulated experiments and field data analysis

Generally, urine mercury (U-Hg) is widely used for assessment of inorganic mercury (I-Hg) exposure in humans. The reliability of scalp hair as an indicator of mercury vapor exposure is contentious. However, significant correlations were found between hair total mercury (T-Hg) and U-Hg and between hair I-Hg and total gaseous mercury (TGM) in ambient air in our previous studies. Simulated experiments were designed to assess the contribution of direct absorption/adsorption of mercury vapor in the hair. Results indicated that the increases of hair T-Hg concentrations were less than 1 μg/g, which was negligible compared with hair T-Hg concentrations in occupationally exposed workers. The β-mercaptoethanol washing can remove 30% of mercury (Hg) in the exposed hair samples. The inhaled Hg constituted the major fraction (97.4%) of I-Hg exposure for the artisanal Hg mining workers. From the simulated experiments and field data analysis, we can conclude that hair I-Hg can be a useful tool for monitoring occupational exposure to Hg vapor.     

Blood total mercury and fish consumption in the Korean general population in KNHANES III, 2005

Objectives

The obejective of this study was to assess the association between the blood total mercury concentration and fish consumption in the Korean general adult population using a representative sample.

Methods

We studied the blood mercury concentration in a representative sample of 1,749 Koreans who were included in the Third Korean National Health and Nutritional Examination Survey (KNHANES III) performed in 2005, and compared it with the frequency of fish consumption collected during the nutrition survey of KNHANES III.

Results

The geometric means of the blood mercury levels in female subjects (n = 890), male subjects (n = 859), and all participants (n = 1,749) representing adult Koreans aged ≥ 20 years were 3.70 μg/L [95% confidence interval (CI), 3.46-3.94 μg/L], 4.70 μg/L (95% CI, 4.38-5.02 μg/L), and 4.15 μg/L (95% CI, 3.93-4.38 μg/L), respectively. The geometric mean of the blood mercury level was significantly higher in the high-fish-consumption group (4.38 μg/L; more than once a week) than in the low-consumption group (3.71 μg/L: less than once a week), but the intergroup difference of 18% was less than that in Western countries. Among the nine listed individual types of fish and shellfish, there was a general trend for the blood mercury level to increase with the consumption frequency of squid, clam, salted seafood, and mackerel. The proportion of Korean women aged 20-49 years having blood mercury levels of at least 5.8 μg/L was 27.7% (150/542) in our study.

Conclusions

The blood mercury level in a representative sample of the Korean adult population was found to be associated with fish consumption in both men and women. However, a high consumption of fish increased the blood mercury level by only 18%.     

The tropical African mercury anomaly: lower than expected mercury concentrations in fish and human hair

Mercury is a neurotoxin and global pollutant, and wetlands and newly flooded areas are known to be sites of enhanced production of monomethylmercury, the form of mercury that is readily biomagnified in aquatic food chains to potentially toxic levels. The Okavango Delta in Botswana, Southern Africa, is the largest inland delta in the world and a wetland ecosystem that experiences dramatic annual flooding of large tracts of seasonal floodplains. The Delta was, therefore, expected to be home to high mercury levels in fish and to be an area where local subsistence fishing communities would be at substantial risk of mercury toxicity from fish consumption. Total mercury concentrations measured in 27 species of fish from the Okavango Delta averaged (mean ± s.d., wet weight) 19 ± 19 ng g⁻¹ in non-piscivorous fish, and 59 ± 53 ng g⁻¹ in piscivorous fish. These mercury concentrations are similar to those reported for fish from lakes in other areas of tropical Africa, demonstrating that not all wetlands are sites of elevated mercury concentrations in biota. Even more intriguing is that concentrations of mercury in fish from across tropical Africa are systematically and substantially lower than those typically reported for fish from freshwater ecosystems elsewhere globally. The reasons for this apparent “African mercury anomaly” are unclear, but this finding poses a unique opportunity to improve our understanding of mercury's biogeochemical cycling in the environment. Mercury concentrations measured in human hair collected in subsistence fishing communities in the Okavango Delta were similarly low (0.21 ± 0.22 μg g⁻¹ dry weight) despite high levels of fish consumption, and reflect the low mercury concentrations in the fish here.     

Mercury and selenium levels in 19 species of saltwater fish from New Jersey as a function of species, size, and season

There are few data on risks to biota and humans from mercury levels in saltwater fish. This paper examines mercury and selenium levels in muscle of 19 species of fish caught by recreational fisherfolk off the New Jersey shore, as a function of species of fish, size, and season, and risk of mercury to consumers. Average mercury levels ranged from 0.01 ppm (wet weight) (Menhaden Brevoortia tyrannus) to 1.83 ppm (Mako Shark Isurus oxyrinchus). There were four categories of mercury levels: very high (only Mako), high (averaging 0.3-0.5 ppm, 3 species), medium (0.14-0.20 ppm, 10 species), and low (below 0.13 ppm, 5 species). Average selenium levels for the fish species ranged from 0.18 ppm to 0.58 ppm, and had lower variability than mercury (coefficient of variation = 38.3 vs 69.1%), consistent with homeostatic regulation of this essential element. The correlation between mercury and selenium was significantly positive for five and negative for two species. Mercury levels showed significant positive correlations with fish size for ten species. Size was the best predictor of mercury levels. Selenium showed no consistent relationship to fish length. Over half of the fish species had some individual fish with mercury levels over 0.3 ppm, and a third had fish with levels over 0.5 ppm, levels that pose a human health risk for high end consumers. Conversely several fish species had no individuals above 0.5 ppm, and few above 0.3 ppm, suggesting that people who eat fish frequently, can reduce their risk from mercury by selecting which species (and which size) to consume. Overall, with the exception of shark, Bluefin Tuna (Thunnus thynnus), Bluefish (Pomatomus saltatrix) and Striped Bass (Morone saxatilis), the species sampled are generally medium to low in mercury concentration. Selenium:mercury molar ratios were generally above 1:1, except for the Mako shark.     

Prenatal exposure to mercury in a prospective mother-infant cohort study in a Mediterranean area, Valencia, Spain

BACKGROUND: Mercury (Hg) is a ubiquitous pollutant that negatively affects fetal and child neurodevelopment at accidental high-dose exposure. Some studies indicate that Mediterranean populations could be at risk of prenatal exposure to mercury through fish consumption. OBJECTIVES: To assess the prenatal exposure to total mercury (T-Hg), both inorganic and organic, in newborns by analyzing the T-Hg concentration in cord blood, and to evaluate the role of maternal fish consumption in this exposure. METHODS: In the context of a multi-center project (INMA project), a prospective birth cohort was set up in Valencia, Spain, from 2005 to 2006. A total of 253 newborns were included in this study. We compared cord blood T-Hg concentration by levels of fish intake assessed by a food frequency questionnaire completed at 28-32 weeks of gestation. Maternal covariates were obtained through a questionnaire. RESULTS: The geometric mean of T-Hg at birth was 9.9 microg/L (95% CI: 9.0, 10.8). Seventy five percent of cord blood samples were above the estimated level assumed to be without appreciable harm (5.8 microg/L). Women who consumed a portion of large oily fish, lean fish, or mixed fried fish two or more times per week had mean cord blood levels 1.6, 1.4 and 1.3 times higher, respectively, than those who rarely or never consumed fish. Other factors such as the mother's age, country of origin, smoking and season of delivery were also significantly and independently associated with cord blood T-Hg concentrations. CONCLUSIONS: Newborns from a Mediterranean area presented elevated levels of T-Hg in cord blood. Higher concentrations of T-Hg were related to maternal fish intake, particularly in the case of large oily fish species.     

An evaluation of mercury levels in Louisiana fish: Trends and public health issues

To characterize statewide fish tissue mercury levels in edible finfish the first comprehensive analysis of Louisiana's fish tissue mercury database was conducted. Analyses were based on fifteen years of fish tissue mercury data collected from 368 waterbodies between 1994 and 2008 (n = 14,344). The overall objectives of this study were to establish baseline fish tissue mercury levels; and evaluate species-specific temporal and spatial trends in fish tissue mercury levels. Fish tissue mercury levels ranged from 0.001 ppm (the detection limit) to 5.904 ppm for king mackerel; with an overall geometric mean of 0.218 ppm. Ninety-five percent of samples had mercury levels below the FDA's action level of 1.0 ppm for methylmercury in commercial food. Forty-four percent of all samples had mercury levels above the U.S. EPA's methylmercury fish tissue criterion of 0.3 ppm for sportfish. Species of potential concern include cobia, king mackerel, blackfin tuna, greater amberjack, spotted bass, bowfin, largemouth bass and freshwater drum. There was a significant but small decline in statewide length-adjusted largemouth bass mercury levels between 1994-1999 to 2003-2008 (p < 0.05). The highest fish mercury levels were observed in Pearl, Calcasieu, Mermentau, Ouachita, Pontchartrain and Sabine basins. Length-adjusted largemouth bass mercury levels were significantly higher in wetlands and rivers/streams vs. lakes; and in wetlands vs. estuaries (p < 0.05). Data were analyzed from a public health perspective to make recommendations for optimizing monitoring and outreach.     

Awake at the switch: improving fish consumption advisories for at-risk women

Eating fish provides health benefits; however, nearly all fish contain at least some methylmercury which can impair human health. While government agencies have been issuing fish consumption advisories for 40 years, recent evaluation efforts highlight their poor performance. The benefit of an advisory can be measured by its ability to inform consumers as to both the positive and negative attributes of their potential choices, leading to appropriate changes in behavior. Because of the health benefits, fish advisories should not reduce fish consumption, even among at-risk individuals, but should lead consumers to switch away from highly contaminated fish toward those less contaminated. Although studies document how advisories reduce fish consumption (a negative outcome), no study indicates whether they lead to switching behavior (a positive outcome).We explore the effects of Maine Center of Disease Control and Prevention's advisory aimed at informing women who may become pregnant, nursing mothers and pregnant women about the benefits and risks of fish consumption. We examine how the advisory changes consumption, especially related to switching behavior. We demonstrate such changes in behavior both during and after pregnancy and compare the advisory-induced changes with those induced by other information sources. Although we find the advisory reduced some women's consumption of fish, we find the decrease is short-lived. Most importantly, the advisory induced appropriate switching behavior; women reading the advisory decreased their consumption of high-risk fish and increased their consumption of low-risk fish. We conclude a well-designed advisory can successfully transform a complex risk/benefit message into one that leads to appropriate knowledge and behavioral changes.     

Mercury exposure and oxidative stress in communities of the Brazilian Amazon

This study was designed to assess possible associations between biomarkers of mercury (Hg) exposure and oxidative stress in fish-eating Amazonian communities. Clinical samples were obtained from riparians living in the Brazilian Amazon. Biomarkers of oxidative stress (glutathione - GSH, glutathione peroxidase - GSH-Px, catalase - CAT, activity and reactivation index of δ-aminolevulinate dehydratase - ALA-D (R%) were determined in blood. Total Hg was measured in whole blood (B-Hg), plasma (P-Hg) and hair (H-Hg). Association between biomarkers of Hg exposure and oxidative stress were examined using multiple regression models, including age, gender, alcohol consumption, smoking status, fish consumption and then stratified for gender. Significant inverse relations were observed between GSH-Px, GSH, CAT, ALA-D activity and B-Hg or H-Hg (p < 0.05). ALA-D reactivation index was positively related to B-Hg (p < 0.0001). P-Hg was directly related to ALA-D reactivation index and inversely associated with GSH-Px, GSH, and ALA-D activity (p < 0.05). When stratified for gender, women showed significant inverse associations between all biomarkers of Hg exposure and CAT (p < 0.05) or GSH (p < 0.05), while for men only P-Hg showed a significant inverse relation with GSH (p < 0.001). Our results clearly demonstrated an association between Hg exposure and oxidative stress. Moreover, for B-Hg, P-Hg and H-Hg gender differences were present.     

Elevated blood selenium levels in the Brazilian Amazon

Contaminated fish poses a difficult challenge throughout the world, on the one hand, fish is a very nutritious food source, while on the other hand it accumulates many toxic substances, including mercury (Hg). As part of our efforts in the Brazilian Amazon to maximize nutritional input from fish consumption, a dietary mainstay, and minimize toxic risk, we have been studying the role of selenium (Se), an essential element, that may influence the distribution of Hg in the body and influence Hg neurotoxicity. Se, which is naturally present in the soil, is ingested through consumption of various foods, notably fish, mammals and certain plants. The objectives of the present study were: (i) evaluate whole blood Se (B-Se) and Hg (B-Hg); (ii) characterize B-Se variations with respect to socio-demographic and dietary variables; and (iii) examine the relation between B-Se and B-Hg. A total of 236 persons from six riparian communities of the Tapajós River Basin, a tributary of the Amazon, participated in this study. Whole blood Se and Hg were measured and interview administered questionnaires were used to obtain data on socio-demographic variable, smoking and drinking habits, and fish and fruit consumption. The results show that B-Se are in the upper normal range (median=284.3 microg/L, range=142.1-2029.3 microg/L). No individuals presented B-Se deficiency, but 9 participants from the same extended family had relatively high B-Se levels, potentially a threat to their health. B-Se varied between communities, was significantly higher among alcohol drinkers and farmers, but not associated with age, sex or tobacco consumption. A significant positive relation between B-Se and B-Hg was noted, independently of the overall fish consumption. B-Se increased with consumption of Peacock bass (Cichla sp.), a piscivorous fish species, and coconut pulp (Cocos nucifera L.). The B-Se intercommunity variations may reflect geographic differences in local soil Se levels as well as traditional land use practices in the different ecosystems of the Tapajós River Basin. In this population with relatively high exposure to Hg, Se may play an important role. Further studies should examine, in this region, the sources of Se, its transmission through the terrestrial and aquatic ecosystem and its role with respect to human health.     

Mercury distribution in waters and fishes of the upper Madeira rivers and mercury exposure in riparian Amazonian populations

In this paper, the results of mercury concentrations in two abiotic compartments (river water and suspended particles) and two biotic compartments (fish and human hair) from the upper Madeira rivers of the Bolivian Amazon basin are presented. Because of the local hydrological regimes and a high deposition rate in the plain, due to the presence of a subsidence zone at the bottom of the Andean piedmont, in the dry season, the highest mercury concentrations and fluxes were not found in rivers where mining activities took place (2.25–6.99 ng l⁻¹; and 1.07–8.67 mg Hg d⁻¹ km⁻²), but at the outlet of the Andean basins exploited for their alluvial gold (7.22–8.22 ng l⁻¹; and 9.47–9.52 mg Hg d⁻¹ km⁻²). The total mercury concentrations measured in surface waters of the upper Beni basin varied during the dry season, from 2.24 to 2.57 ng l⁻¹ in the glacial waters of the Zongo river, to 7.00 ng l⁻¹ in the Madeira River at Porto Velho and 9.49–10.86 ng l⁻¹ at its confluence with the Amazon. The results obtained from fish indicate, on one hand, that 86% of the piscivorous fishes collected in the Beni river were contaminated, and, on the other hand, their high mercury concentrations could exceed by almost four times the WHO (1976) safety limit. In the Beni River, the mercury concentrations found in omnivorous and mud-feeding fish ranged from 0.02 to 0.19 μg g⁻¹ (wet wt.), and in piscivorous fish, from 0.33 to 2.30 μg Hg g⁻¹ (wet wt.). The mercury accumulated by carnivorous fishes was mainly present in its organic form; methylmercury represented 73–98% of the total mercury analysed. Eighty persons were studied in the entire Bolivian Amazonian basin. Unlike the gold miners, who are more affected by tropical diseases, such as malaria and yellow fever, the indigenous people living on the banks of the Beni river, present elevated levels of mercury (9.81 μg g⁻¹ on average). We observed an increase in contamination in young children still being breast-fed, confirming that hair mercury concentration in babies was significantly affected by maternal mercury contamination during pregnancy. These results show that the major health impacts caused by mercury affect people who are not working directly in gold mining activities but who have a regular fish diet.     

Mercury concentration in meconium and risk assessment of fish consumption among pregnant women in Taiwan

Meconium is a matrix that can be obtained easily and noninvasively and is useful for detecting antenatal fetal exposure to environmental toxins. Taiwan is an island with high fish consumption, and many pregnant women would like to enjoy the benefits of fish without jeopardizing their health or that of their child. The aim of this study is to assess the mercury concentration in meconium in relation to the health risk of mercury exposure. A total of 198 mother-infant pairs residing in the city of HsinChu were recruited for the study between January 2007 and June 2007. The average mean concentration of mercury in meconium was 79.2 ± 7.3 ng g^− 1 dry wt We use the Monte Carlo technique to assess the uncertainty in risk assessment and the impact of these uncertainties on the estimation of expected risk of mercury intake from fish in mothers. Based on the FAO/WHO's tolerable daily intake of methylmercury (0.23 μg kg^− 1 d^− 1), we found that 17.3% and 14.0% of the daily mercury exposure estimated exceeded the reference dose for foreign-born and Taiwan-born mothers, respectively. We found that the mercury concentration in meconium was much higher than in other studies, except for one study done in Tagum in the Philippines where mercury is used in gold mining. This may be because Asia is the largest emitter of anthropogenic mercury, accounting for 53% of worldwide emissions. Sensitivity analysis suggests that mercury concentration in fish and the rate of ingesting fish may be the key parameters for governments offering risk management guidance to protect the health of mothers and unborn babies.     

Blood levels of organotin compounds and their relation to fish consumption in Finland

The objective of this study was to measure the concentrations of organotin compounds in the whole blood of Finnish male fishermen (n=133), their wives (n=94), and other family members (n=73), and to investigate their associations with background variables. The concentrations were generally low, less than the limit of quantification (LOQ) for the vast majority of compounds and samples. Of the organotin compounds (mono-, di-, and tributyltin, mono-, di-, and triphenyltin, and dioctyltin), only triphenyltin was detected in more than just a few samples (in 37 of 300 samples, LOQ=0.04 ng/ml). These were mainly the samples of fishermen (26/37) and their wives (10/37). For statistical analysis, concentrations of triphenyltin were divided into two categories, LOQ. Of the different background variables, age and fish consumption contributed the most to the triphenyltin concentrations. When age and fish consumption (g/day) were divided into three categories, odds ratios comparing the highest with the lowest category were 3.88 for age (95% CI 1.36-11.09) and 3.48 for fish consumption (1.36-8.94), respectively. Compared with females, males had an odds ratio of 1.51 of having the concentration of triphenyltin >LOQ (0.72-3.14). To the best of our knowledge, this study confirmed for the first time with human samples that fish consumption can be associated with triphenyltin concentration in whole blood.     

Burdens of mercury in residents of Temirtau, Kazakhstan: I: Hair mercury concentrations and factors of elevated hair mercury levels

Mercury (Hg) is released either naturally in the environment or by anthropogenic activities. During its global circulation, Hg presents in a diversity of chemical forms and transforms between each other. Among Hg species, methylmercury (MeHg) is readily absorbed by humans via the aquatic food chain and thus it is very neurotoxic to exposed populations including fetuses due to perinatal exposure. In 2005, a survey was carried out in Temirtau, an Hg-contaminated site in North Central Kazakhstan, to investigate Hg concentrations in the hair samples of the residents and the relationship between Hg exposure levels and the related factors. Among the 289 hair samples, Hg concentrations ranged from 0.009 to 5.184 µg/g with a mean of 0.577 µg/g. Nearly 17% of the population exceeded 1 µg/g for hair Hg, which corresponds to the reference of dose (RfD) 0.1 µg/kg body weight/day developed by the United States Environmental Protection Agency (USEPA). Subgroups of males, people aged over 45 and fishermen or anglers were found to have elevated Hg exposure levels in their hair. A positive correlation was found between Hg concentrations in hair and frequencies of river fish consumption. As a result, the finding that people were exposed to high levels of Hg was expected due to the frequent consumption of fish caught from the polluted River Nura or the neighbouring lakes. A regression model showed that approximately 41% of variance of Hg concentrations in the study population's hair was attributed to the variables of gender, residential location, age and fishery occupation. The model implied that demographic characteristics together with dietary behaviour should be taken into account in studies associated with Hg exposure risk, in order to clearly define the group potentially sensitive to Hg exposure.     

Occupational and environmental mercury exposure among small-scale gold miners in the Talensi-Nabdam District of Ghana's Upper East region

Mercury use in small-scale gold mining is ubiquitous across Ghana but little is known about the extent to which such activities have contaminated community residents and miners. Here, occupational exposures to elemental mercury (via urine sampling) and dietary exposures to methylmercury (via hair sampling) were assessed among 120 participants recruited from a mining community located in the Talensi-Nabdam District of Ghana's Upper East region during summer 2009. More than one-fifth of the participants had moderately high levels of urinary mercury (> 10 μg/L) and 5% had urine mercury levels that exceeded the WHO guideline value of 50 μg/L. When participants were stratified according to occupation, those active in the mining industry had the highest mercury levels. Specifically, individuals that burned amalgam had urine mercury levels (median: 43.8 μg/L; mean ± SD: 171.1 ± 296.5 μg/L; n = 5) significantly higher than median values measured in mechanical operators (11.6 μg/L, n = 4), concession managers/owners (5.6 μg/L, n = 11), excavators that blast and chisel ore (4.9 μg/L, n = 33), individuals that sift and grind crushed ore (2.2 μg/L, n = 47), support workers (0.5 μg/L, n = 14), and those with no role in the mining sector (2.5 μg/L, n = 6). There was a significant positive Spearman correlation between fish consumption and hair mercury levels (r = 0.30) but not with urine mercury (r = 0.18) though further studies are needed to document which types of fish are consumed as well as portion sizes. Given that 200,000 people in Ghana are involved in the small-scale gold mining industry and that the numbers are expected to grow in Ghana and many other regions of the world, elucidating mercury exposure pathways in such communities is important to help shape policies and behaviors that may minimize health risks.     

Fish mercury development in relation to abiotic characteristics and carbon sources in a six-year-old, Brazilian reservoir

Time series on fish mercury (Hg) development are rare for hydroelectric reservoirs in the tropics. In the central-western part of Brazil, a hydroelectric reservoir, called Lago Manso, was completed in 1999 after that background levels of fish Hg concentrations had been determined. The development for the first 3 years was studied in 2002. The objective of the present study was to determine development of fish Hg concentrations for a second three-year period after flooding. The bioaccumulation factor and certain abiotic and biotic factors, possibly affecting the availability and accumulation of Hg, were also examined. The results show that Hg levels in fish from Lago Manso have increased more than five times compared to the background levels observed before construction of the reservoir. At the same time, dissolved organic carbon has increased while dissolved oxygen has decreased indicating enhanced bioavailability of Hg. In the reservoir, Salminus brasiliensis had in average a Hg content of 1.1 microg g(-1) f.w., Pseudoplatystoma fasciatum 1.2, Serrasalmus marginatus/spilopleura 0.9, and Brycon hilarii 0.6 microg g(-1) f.w. The average fish Hg contents were higher downstream, except for B. hilarii. In the reservoir, the average Hg content of each species was in 2005 always over the consumption limit (0.55 microg total Hg g(-1) f.w.) recommended by WHO. Therefore, the people living around Lago Manso should be informed of the health effects of Hg, and fish consumption recommendations should be carried out. The accumulation of Hg varies widely between species as shown by the bioaccumulation factor which ranges between 5.08 and 5.59 log units. The observed variation is explained by differences in diet and trophic position with piscivorous fish exhibiting the highest mean Hg concentration, followed by carnivorous and omnivorous species. Carbon isotope analyses imply that trophic position is not the only cause of the observed differences in Hg levels between omnivorous B. hilarii, having a diet partly based on C(4) plants, and carnivorous S. marginatus as well as piscivorous S. brasiliensis, whose carbon sources are depleted in (13)C. The fact that the species have different carbon sources indicates that they belong to different food chains.     

Pesticide concentrations in maternal and umbilical cord sera and their relation to birth outcomes in a population of pregnant women and newborns in New Jersey

We evaluated in utero exposures to pesticides by measuring maternal and cord serum biomarkers in a New Jersey cohort of pregnant women and the birth outcomes of their neonates. The study was based on 150 women that underwent an elective cesarean delivery at term in a hospital in central New Jersey. We evaluated the following pesticide compounds in both maternal and umbilical cord sera: chlorpyrifos, diazinon, carbofuran, chlorothalonil, dacthal, metolachlor, trifluralin and diethyl-m-toluamide (DEET). Of these compounds, chlorpyrifos, carbofuran, chlorothalonil, trifluralin, metolachlor and DEET were the pesticides most frequently detected in the serum samples. We found high (≥75th percentile) metolachlor concentrations in cord blood that were related to birth weight (3605 g in upper quartile vs 3399 g; p = 0.05). We also observed an increase in abdominal circumference with increasing cord dichloran concentrations (p = 0.031). These observations suggest that in utero exposures to certain pesticides may alter birth outcomes.