阳离子型聚丙烯酰胺絮凝马铃薯深加工工艺蛋白废液的研究

[目的]研究阳离子型聚丙烯酰胺絮凝马铃薯深加工工艺蛋白废液的最佳条件.[方法]以3%聚丙烯酰胺为絮凝剂,研究不同温度、pH、絮凝剂用量、处理时间等对马铃薯深加工的废液絮凝效果的影响.[结果]每100 ml马铃薯深加工工艺蛋白废液,在3%聚丙烯酰胺用量为8 ml、pH为5.5、处理时间为36 h、温度为30℃条件下进行絮凝,有机污染物(COD<,Cr>)的沉降率可达53.69%.[结论]有机絮凝剂阳离子型聚丙烯酰胺能有效絮凝沉淀马铃薯深加工工艺蛋白废液中的大量有机污染物,达到用微生物净化去污需要的目标要求.     

仁用杏微卫星非变性聚丙烯酰胺凝胶电泳体系的优化

[目的]优化仁用杏微卫星非变性聚丙烯酰胺凝胶电泳体系.[方法]以25份仁用杏和3份食用杏为材料,利用微卫星标记结合非变性聚丙烯酰胺凝胶电泳技术筛选出多态性高、可重复的SSR位点.并比较不同因子对电泳体系的影响,探索仁用杏微卫星非变性聚丙烯酰胺凝胶电泳的优化体系.[结果]仁用杏微卫星非变性聚丙烯酰胺凝胶电泳的优化体系为:聚丙烯酰胺凝胶的浓度为6%,丙烯酰胺与甲叉双丙烯酰胺的比例为29∶1,1 000 V下电泳2～3 h,0.1% AgNO3 染色15 min 后显影.[结论]该优化体系为仁用杏资源微卫星标记遗传分析奠定了技术基础.     

魟鱼软骨多糖的聚丙烯酰胺凝胶电泳鉴别

[目的]用聚丙烯酰胺凝胶电泳对虹鱼软骨多糖(RCG)进行定性鉴别.[方法]利用纯化RCG,进行聚丙烯酰胺凝胶电泳.[结果]RCG经纯化,基本实现了多糖的分离;根据聚丙烯酰胺凝胶电泳迁移率,可明显鉴别出魟鱼RCG.[结论]该法重现性较好,简单易行,可用于魟鱼RCG的定性鉴别.     

兰州百合与亚洲百合及其杂种F1的蛋白质和同工酶分析

[目的]为选配亲本组合及杂种早期鉴定提供理论依据.[方法]采用聚丙烯酰胺凝胶电泳技术,对兰州百合与亚洲百合及杂交后代的可溶性蛋白质和过氧化物酶进行分析.[结果]以兰州百合为亲本的杂交后代的蛋白质谱中不仅出现了与亲本同源但比亲本染色加深的带,且出现了亲本中不存在的新带.杂种F1 过氧化物酶谱主要表现为亲本的不完全互补型和杂种型.[结论]蛋白质谱和过氧化物酶谱可作为百合杂种鉴定的生化指标及进行目标性状植株的检测.     

嗜盐古菌Haloferax sp.H4蛋白质表达差异研究

[目的]研究嗜盐古菌Haloferax sp.H4在不同环境中的蛋白表达差异.[方法]采用变性聚丙烯酰胺凝胶电泳(SDS-PAGE)检测不同NaCl浓度、pH、温度、有氧和无氧条件下的H4菌株蛋白表达差异.[结果]H4菌株在不同NaCl浓度、有氧和无氧条件下的蛋白表达存在明显特异性;而在不同pH和温度条件下,其蛋白表这差异性不明显.[结论]嗜盐古菌对不同环境因子的调控方式可能存在差异.     

内蒙古地区罗布麻POD同工酶分析

[目的]时内蒙古不同地区罗布麻POD同工酶谱进行比较分析,为该地区罗布麻的育种利用及亲缘关系的鉴定分析提供依据.[方法]采用聚丙烯酰胺凝胶垂直板电泳技术,研究了内蒙古通辽、巴彦淖尔、包头3个地区罗布麻子叶期叶、茎、根的POD同工酶酶谱特征.[结果]内蒙古3个地区罗布麻苗期叶、茎、根POD同工酶酶带的位置、数目和强弱均存在一定的差异.3个地区罗布麻叶、茎、根POD同工酶酶谱共有44条带,可明显划分为1和2区,其中强带集中在2区,占总强带的79%;而中强带和弱带主要集中在l区,分别占中强带和弱带总数的75%.[结论]内蒙古3个地区罗布麻遗传多样性比较丰富.     

基于EST同工酶的缘蝽亚科10种缘蝽系统发育关系初探(半翅目:缘蝽科)

[目的]探讨缘蝽亚科棘缘蝽属、黑缘蝽属、瘤缘蝽属、缘蝽属和同缘蝽属10种昆虫系统发育关系.[方法]采用聚丙烯酰胺凝胶电泳技术研究秦巴山区缘蝽亚科10种缘蝽的EST同工酶.[结果]聚类分析结果表明,缘蝽亚科5属不同属间差异要大于属内各种间差异,同缘蝽属与其他4属亲缘关系较远,与巨缘蝽亚科亲缘关系较近.L结论]该亚科10个种EST同工酶谱带清晰、多态性强、稳定性好,适用于缘蝽亚科昆虫鉴别和物种系统发育研究.结合形态特征及Cyt b基因序列变异情况,建议将同缘蝽属从缘蝽亚科划入巨缘蝽亚科.     

甜荞和苦荞品种遗传多样性的RAPD分析

[目的]对甜荞和苦荞品种遗传多样性进行RAPD分析.[方法]以7个随机引物对贵州省1999～2010年荞麦区甜荞和苦荞参试品种及其亲本等合计19个品种进行RAPD分析.[结果]共获得149条DNA扩增带,其中多态性谱带141条,多态性谱带的平均比率为94.89%.多态性分析及聚类分析表明,供试品种彼此均有一定的差异,其中威宁甜荞品种彼此亲缘关系较近,而其他甜荞品种间关系较远.遗传变异性程度以种问差异最大,其次是甜荞种内不同品种间,而苦荞种内不同品种间的遗传变异性最小.[结论]研究初步建立了19个品种的RAPD指纹图谱.     

番茄抗花叶病毒基因类似序列扩增研究

[目的]研究番茄抗花叶病毒基因类似序列扩增.[方法]以选育和生产上广泛使用的20个番茄品种为试材,对其进行了田间和实验室抗花叶病毒病(ToMV)筛选、基因类似序列扩增、RAPD聚类分析及特异引物PCR扩增.[结果]美味樱桃番茄、中杂9号、旱红宝、W2624、OH-2-2-11、黄圣果和美国番茄对ToMV具有较强的抗性;根据聚类分析,受试品种可分为3类,从中选出9个抗性和非抗性品种进行抗ToMV,基因类似序列分析,其中非抗性和购买无抗性番茄品种未扩增出任何谱带,而抗性品种扩增出约300 bp的谱带,初步认为该谱带为抗ToMV类似序列基因.[结论]为培育抗花叶病毒病的番茄品种提供了理论依据.     

碱溶时间对大豆蛋白提取率和得率的影响

[目的]改进提取大豆蛋白质的工艺,取得碱溶时间的最大简单效应.[方法]采用单因素分析方法研究提取大豆蛋白工艺中碱溶时间对大豆蛋白提取率及得率的简单效应.[结果]在料水比1:10(M:V,g/ml)、酸沉pH值4.3、离心速度4 000 r/min、碱溶pH值9.5、碱溶时间60 min的条件下,平均蛋白提取率最高,为66.51%,与碱溶时间为50 min时的平均蛋白提取率65.81%差异不显著.同样条件下,平均蛋白得率最高,为29.74%,与碱溶时间为50 min时的平均蛋白得率29.42%差异不显著.[结论]大豆蛋白质工艺提取中宜选择50 min作为碱溶时间的参数.     

转炉炉壳热应力分析

根据实际转炉建立实体模型。以有限元为手段,考虑了炉衬和炉壳材料的物性参数随温度变化的特点和炉衬与炉壳之间膨胀间隙,计算了转炉炉壳在温度载荷和炉衬膨胀压力同时作用下的热应力。所得结果可为转炉炉壳设计提供依据。     

焦炭热性能指标意义浅析

通过对焦炭在高炉内的历程及其变化规律的剖析,阐述焦炭热性能指标的意义,形象地把焦炭在高炉内的损耗分为三部分(M1,M2,M3)。指出M2对焦比及焦炭价格有一定的指导作用。     

Results of treatment of cancer of the cardia

Cancer of the cardia is traditionally discussed with cardiac cancer of the lower portion of the esophagus and upper gastric cancer invading the esophagus, and the specific characteristics of cancer of the cardia have never been clearly defined. We reviewed the outcome of 172 patients with adenocarcinoma of the cardia who had undergone radical surgery between 1949 and 1994 in the Division of Gastrointestinal Surgery of the Cancer Institute Hospital. The centers of the tumors were located within 2 cm above and below the boundary between the esophagus and the stomach, and their longitudinal diameter was less than 8 cm. We divided the patients into an early period (1949-1979; n = 79) and a late period (1980-1994; n = 93), and focused on the historical transition. There were no differences in patient gender or histology between the two periods. However, the late period was associated with fewer cases of esophageal invasion and shorter longitudinal diameter, as the age of the population advanced. The number of advanced cancers such as the localized and infiltrative type had decreased, and early cancer and early clinical stage had become more common, but despite the fact that the number of early cancers had increased, extended dissection, such as thoracic and paraaortic lymph node dissection, was performed more frequently. By clinical stage, the long-term outcome markedly improved in Stage I patient in the late period, and tended to improve in Stage II and III patients. This appears to have been attributable to the prevention of micrometastasis by extended dissection, although the number of early cancers is another major potential cause. There were no differences in the outcome of Stage IV patients between the two periods, and further advances in multimodality therapy must be awaited. The range of resection is basically proximal gastrectomy, and if there are adequate indications, the prognosis is favorable. In view of the status of lymphatic flow and lymph node metastasis, and long-term results, lateroaortic lymph node dissection is important. Since the number of early cancer patients has been increasing, if intraabdominal recurrence is prevented, intrathoracic lymph node dissection will contribute greatly to the outcome of such patients.     

The effect of amount of possession of the trait of hostility on accuracy of perception of hostility in others.

Some theories of personality hold that an individual's perception of others is influenced by his own personality characteristics; more specifically, that a trait of low social value will be projected more into others, and that insight into the possession of these characteristics reduces this distortion. These 2 hypotheses were tested by having a group of college students rate each other (including themselves) on degree of friendliness or hostility. Agreement with group rating about self yielded the insight score. In general, the hypotheses were supported; the "friendly" Ss were most accurate in their perception of others, and insight was positively associated with accurate perception. From Psyc Abstracts 36:04:4HL16M. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Comparison of rates of infection of two methods of emergency ventricular drainage

The rates of infection of two methods of external ventricular drainage in use at Atkinson Morley's Hospital--namely, (a) percutaneous drainage with Rickham reservoirs and (b) tunnelled ventriculostomies--were compared in this retrospective review. Percutaneous drainage of CSF with Rickham reservoirs was associated with a 27% rate of infection as identified by positive microbiological cultures; tunnelled ventriculostomy catheters had a 10% infection rate. The difference in the infection rate between the two methods was statistically significant (P < 0.015). Other variables examined, including the age and sex of the patients and the reasons for ventricular drainage, were not associated with an increased rate of infection. Most infections from either method were caused by a coagulase negative staphylococcus. The average duration of ventricular drainage before identification of positive cultures was 5.7 days for Rickham reservoirs and 6.0 days for ventriculostomies.     

浅析《野草》的版画特征

鲁迅先生一生热爱美术作品,对版画中木刻兴趣尤甚.<野草>作为其"写心之作",亦呈现出一些与木刻版画一致的审美特征:一是具有木刻版画的色彩特征,多对比、多冷色,有阴郁的美感;二是具有木刻版画的力度特征,环境描写、人物刻画处处刀锋毕现,遒劲有力,有一种"力之美".     

四辊破碎机车刀架的改造

针对四辊破碎机车刀架车削中存在的几个问题,详细介绍了电动车刀架的结构和工作原理,建立主要设计参数的数学表达式,并进行适当的分析。改造后,这种电动车刀架运行平稳、可靠、方便、省力,取得了预期的效果。     

Effect of antibiotics of chemotaxis of human leukocytes

The effect of 20 different antibiotics on chemotaxis by human neutrophils was studied. Human leukocytes incubated with chloramphenicol, rifampin, sodium fusidate, and tetracyclines in vitro showed markedly depressed migration. The mechanisms by which these antibiotics affect leukotaxis are discussed.