红缘短鼻蝗染色体C带核型研究(蝗总科:癞蝗科)

[目的]研究红缘短鼻蝗的染色体C带核型.[方法]利用BSG法进行显带处理,显微摄影后,测量染色体和C带带纹的相时长度,计算每条染色体异染色质的含量.[结果]红缘短鼻蝗的染色体组成为2n =19,性别决定机制是XO型,染色体全部为端着丝粒染色体,且仅具有着丝粒C带带纹.此外,异染色质在染色体组中的总含量为16.40%.[结论]该研究为癞蝗的细胞遗传学和细胞分类学提供了染色体及其显带方面的资料.     

暗紫红毛菜ITS序列的测定与分析

[目的]对暗紫红毛菜rDNA内转录间隔区(ITS区)进行序列测定,为其系统发育研究提供新资料.[方法]以产于山西娘子关泉的一株暗紫红毛菜为材料,提取DNA,设计引物,进行PCR扩增,进而测定其ITS区基因序列.[结果]暗紫红毛菜与同科的少精紫菜ITS区同源性为75%,与条斑紫菜ITS区同源性为79%.[结论]ITS区序列进化速率相对较快,种类和地理分布的差异是其序列差异产生的原因.     

草莓属植物染色体核型分析

[目的]分析二倍体野生种草莓的核型,为探讨草莓的起源、进化及遗传育种研究提供依据.[方法]对草莓属(Fragaria)植物4个二倍体种森林草莓(F.vesca L.)、纤细草莓(F.gracilis A.Los.)、黄毛草莓(F.nilgerrenis Schidl)和五叶草莓(F.pentaphylla A.Los.)进行了核型分析.[结果]纤细草莓的核型为2n=2x=14=10m+2sm+2m*,属1B类型;黄毛草莓为2n=2x=14=14m,属IA类型;五叶草莓为2n=2x=14=12m+2sm,属1A类型;森林草莓为2n=2x=14=14m,属lA类型.[结论]草莓二倍体及品种核型对称性为:黄毛草莓>森林草莓>五叶草莓>纤细草莓,供试草莓种的亲缘进化顺序可能为黄毛草莓、森林草莓、五叶草莓、纤细草莓.     

基于SSR分子标记的宁粳28籼粳成分分析

[目的]为杂交水稻育种提供科学依据.[方法]以水稻品种宁粳28为试验材料,选取分布于水稻12个连锁群上的136对SSR引物,以2个测序品种日本晴和9311分别为典型粳稻与籼稻标准,利用SSR分子标记技术分析3个品种在DNA水平上的多态性差异,研究宁粳28粳稻与籼稻成分在染色体上的分布.[结果]宁粳28叶片DNA的PCR扩增产物经5%琼脂糖凝胶电泳后,16个标记住点为偏籼稻带型(大多数位点分布于3、5、8号染色体上),4个标记位点无多态性,8个标记住点为籼粳中间型,其余107个标记位点为偏粳稻带型,[结论]宁粳28为典型的粳稻,其籼稻成分只占被抽查住点的9.56%.     

1株出芽短梗霉的分离·纯化及鉴定

[目的]为出芽短梗霉的进一步开发利用提供理论依据.[方法]采用平板稀释法从土壤中分离出1株出芽短梗霉,用YPD培养基培养菌丝体,显微成像获得菌丝微观形态,利用分子生物学方法对其rRNA基因内转录区段(ITS 区)进行克隆测序,并与Genbank中已知序列进行比较.[结果]ITS区PCR扩增产物测序结果表明,所扩增序列的长度为606 bp,与Genbank中短梗霉的同源率为98%～99%,与Aureobasidium pullulans wb 149、A.pullulans HK58-1(2)属于同一单独分枝;形态及分子鉴定结果表明,出芽短梗霉培养成功.[结论]该研究采用经典与现代分子技术相结合的方法鉴定出了1株出芽短梗霉,为出芽短梗霉的分类鉴定提供了依据.     

Taqman定量PCR技术检测转基因大豆中外源基因拷贝数

[目的[采用Taqman定量PCR技术检测转基因杂交大豆中外源nos终止子基因的拷贝数.[方法]以大豆凝集素基因为内参照基因,以非转基因大豆基因组DNA为内参照基因标准品,通过梯度稀释法分别求取了内参照基因和质粒DNA的Ct值与拷贝数对数值的相关性标准曲线方程,并通过将得到的Ct值代入标准曲线方程求取了样品的拷贝数.[结果]内参照基因标准曲线方程为y=-3.422x+35.201,R2=0.998;外源基因标准曲线方程为y=-3.348x+34.890,R2=0.999.nos终止子基因及其下游边界序列在转基因杂交大豆中为单拷贝.[结论]为确定转基因大豆外源基因拷贝数提供了理论依据.     

纯血马和百色马IGF1R基因蛋白编码区序列的多态性分析

[目的]研究纯血马和百色马IGF1R基因成熟蛋白编码区序列的多态性,为揭示马矮小性状的分子机理和马的分子育种提供理论参考.[方法]采集纯血马和百色马的血样各57份,按常规酚仿法抽提DNA.利用DNA混合池和PCR产物直接测序的方法检测IGF1R基因的多态位点,利用PCR-RFLP技术分析这些多态位点在纯血马和百色马中的基因型及其频率分布.[结果]在马IGF1R基因的第2、第5和第16外显子上存在4个多态位点,其中突变179 627 T→C为纯血马和百色马共有,且两者在基因型频率上差异不显著;突变212 077 G→A为纯血马所独有;突变406 T→C和212 110 G→A为百色马所独有.[结论]马IGF1R基因的多态位点可能与百色马的矮小和纯血马的高大有关.     

野大麦盐胁迫rbcS基因的克隆及序列分析

[目的]对野大麦盐胁迫rbcS基因进行克隆及序列分析.[方法]以盐胁迫下的野大麦幼嫩叶片为试材,根据GenBank中小麦和大麦rbcS基因核酸序列的同源性设计引物,对野大麦基因组DNA进行PCR扩增、回收、连接、转化及测序.[结果]在野大麦的基因组中克隆了2个大小不同的rbcS基因序列rbcS1和rbcS2,长度分别为1 252和908 bp.rbcS1和rbcS2均由2个外显子和1个内含子组成,外显子长度相等,编码序列为525 bp,同源性为96%,编码174个氨基酸;rbcS1和rbcS2内含子大小不同,分别为448 和107 bp.[结论]为进一步探讨rbcS基因在野大麦耐盐机制中的分子机制奠定了基础.     

青海野生与栽培麻花艽psbA-trnH序列的比较

[目的]分析野生与栽培麻花艽(Gentiana straminea Maxim.)psbA-trnH 序列的差异,为麻花艽的基源鉴定和品质评价提供分子依据.[方法]采用PCR 扩增纯化后直接测序的方法,测定野生与栽培麻花艽 cpDNA psbA-trnH 核苷酸序列并作序列同源性分析.[结果]野生与栽培麻花艽的cpDNA psbA-trnH 片段长度分别为316和317 bp,两者之间有4个碱基的变异.[结论]利用psbA-trnH 区序列的差异可以鉴别野生与栽培麻花艽.     

番茄抗花叶病毒基因类似序列扩增研究

[目的]研究番茄抗花叶病毒基因类似序列扩增.[方法]以选育和生产上广泛使用的20个番茄品种为试材,对其进行了田间和实验室抗花叶病毒病(ToMV)筛选、基因类似序列扩增、RAPD聚类分析及特异引物PCR扩增.[结果]美味樱桃番茄、中杂9号、旱红宝、W2624、OH-2-2-11、黄圣果和美国番茄对ToMV具有较强的抗性;根据聚类分析,受试品种可分为3类,从中选出9个抗性和非抗性品种进行抗ToMV,基因类似序列分析,其中非抗性和购买无抗性番茄品种未扩增出任何谱带,而抗性品种扩增出约300 bp的谱带,初步认为该谱带为抗ToMV类似序列基因.[结论]为培育抗花叶病毒病的番茄品种提供了理论依据.     

Laudatio: Prof. Dr. med. Dr. phil. Dres. h. c. Heinz H?fner

During the course of a year, there are many healthcare workers nationwide who sustain sharps injuries whilst carrying out their duties. Staff who work in the Accident and Emergency (A & E) units are no different from anyone else in this respect. The emotional cost of such an accident cannot be calculated, whereas the financial costs can. This article aims to show how the cost of an injury to a member of staff can be calculated, thus allowing a manager to assess the financial implications of sharps injuries to their department. In the absence of access to an Occupational Health (OH) department, most A & E units have to respond to sharps injuries to healthcare workers in the hospital. This task must be attended to by the medical staff as well as the nursing staff of the A & E unit. Within the following text the appropriate responses and methods to ensure uniformity of response are discussed. The tables and the calculation figures shown are taken directly from the information held in the St James's University Hospital Trust's OH department. All calculations are based on the middle band of the salary scale for each discipline. Accurate costings for each member of staff injured cannot be shown in this article, but the general principle is laid down and can be easily followed for any situation.     

1969 C.P.A. Institute.

Presents the program for the C.P.A. Institute entitled "The Role of Counseling Services on the Contemporary Campus." The Institute was held on June 2-3, 1969. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Rats, Man . . .

Comments on the article by Richter entitled "Rats, Man, and the Welfare State" (see record 1960-02810-001). The commenter notes that in his article Richter did not indicate how hereditary and environmental factors were controlled. In comparing the hereditary survival characteristics of wild versus domesticated rats, it would be essential that the environmental influences on the survival characteristics of the wild rats should be ruled out. Another factor to be considered is that the wild state may offer a diet and other environmental influences, which in the life of a single individual would produce organic changes, accounting for some of the differences between wild and domestic rats. It would seem that the most effective way to determine the hereditary differences between wild and domestic rats would be to create a wild group, composed solely of the offspring of wild rats. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

The blood-brain barrier. I. Histology. Physiology. Embryology. Transport

The concept of blood-brain barrier (bbb) arose in the beginning of the 20th century after report that the dyes injected intravenously did not appear in the brain. The idea and definition of bbb has been changed many times since then. We present current opinion concerning bbb, its histology, embryology, ultrastructure and function on the ground of a review of the recent literature.