醇法生产花生浓缩蛋白的工艺条件研究

以脱脂花生蛋白粉为原料,通过单因素实验和正交实验优化乙醇浸提法生产花生浓缩蛋白的工艺条件,并通过放大实验进行验证。结果表明,花生浓缩蛋白生产的最佳工艺条件为:乙醇体积分数65%,料液比1∶8,浸洗温度60℃,浸洗时间40 min,浸洗次数3次。在最佳工艺条件下所得花生浓缩蛋白产品的蛋白质含量为72%,氮溶解指数为42%。100 g放大实验所得花生浓缩蛋白产品的蛋白质含量为70%,氮溶解指数为40%。     

花生浓缩蛋白的制备工艺研究

以低温花生粕为原料,采用醇洗法制备花生浓缩蛋白,研究了乙醇溶液体积分数、浸洗温度、浸洗次数及固液比对产品中蛋白质含量的影响.通过正交实验确定的最佳工艺条件为:乙醇溶液体积分数60%,浸洗温度50℃,固液比1∶8,浸洗次数5次(每次30 min).在该条件下制备的产品中粗蛋白含量为68.15%(N×6.25,干基),氮溶解指数(NSI)为32.72%.     

冷榨花生饼混合溶剂萃取生产浓缩蛋白工艺条件的研究

本文以冷榨花生饼为原料,采用混合溶剂浸洗工艺制取花生浓缩蛋白。通过单因素试验和正交试验得到的最佳工艺条件为：溶剂比（正己烷：乙醇）3．5：6．5,乙醇浓度75％,时间60min,温度50℃,料液比1：11,萃取4次。在此条件下所得产品的粗蛋白含量70．50％,残油率（1．52％,NSI值20．78％。混合溶剂浸洗工艺较单纯的醇洗工艺所得产品的蛋白质含量、功能性及残油率显著改善,这对拓展花生浓缩蛋白在食品业的应用有重要意义。     

多级逆流醇浸法制取大豆浓缩蛋白工艺的研究

大豆浓缩蛋白具有比脱脂蛋白粉蛋白质含量高和比大豆分离蛋白价格低的优势,广泛应用于各种食品体系中.目前,世界上大豆浓缩蛋白的生产方法主要是乙醇浸洗法,本文采用多级逆流醇浸法,对影响大豆浓缩蛋白的工艺条件,如浸取温度、料溶比、乙醇溶液的浓度和浸取时间进行了较为详尽的研究.通过正交实验及结果分析,确定了其最佳的工艺条件为为:浸取温度60℃,料溶比1:5,乙醇溶液浓度60%,浸取时间30min.     

醇法大豆浓缩蛋白制取工艺的探讨

采用稀浓乙醇两次浸出 ,一次脱溶的工艺生产醇法大豆浓缩蛋白 ;实验研究考察了乙醇浓度、浸出温度、浸出时间、固液比对产品质量的影响。通过正交实验 ,选择的最佳生产工艺条件为 :乙醇浓度 75 %、浸出温度 30℃、浸出时间 6 0min、固液比 1∶5。二次浸出工艺条件为 :乙醇浓度90 %、浸出温度 5 0℃、工作时间 30min、固液比 1∶5。     

酶解醇洗法制备棉籽浓缩蛋白

以预榨浸提棉籽粕为原料,先选用戊聚糖复合酶、糖化酶等植物水解酶对棉籽粕进行作用,再进一步用乙醇溶液浸洗制备棉籽浓缩蛋白.结果表明:在添加糖化酶1600 U/g、戊聚糖复合酶0.60 fbg/g、料液比1 ∶ 10和温度50℃条件下酶作用140 min后,再以料液比1∶7、体积分数70％乙醇溶液和温度60℃条件下醇洗2次,每次45 min,得到产品浓缩蛋白的蛋白质质量分数为64％,蛋白质收率为78％.     

速溶苦丁茶的研制

研究了苦丁茶超细粉碎时间对粉碎效果的影响和有效成分在水中的溶出特性 ,萃取液添加适当配料后经浓缩、干燥制得速溶苦丁茶粉末。结果表明制备速溶苦丁茶的几个重要工艺参数是 :超细粉碎时间为 12～ 15分钟 ,萃取时间为4 5～ 5 0分钟 ,茶水比为 1∶ 4 0。     

大理百合多糖的醇沉分离工艺及脱蛋白方法

筛选出最佳的大理百合多糖的醇沉分离工艺及脱蛋白方法;比较在不同提取液浓缩比和乙醇加量条件下的多糖沉淀率,筛选出最佳的提取液浓缩比和乙醇加量。从蛋白质清除率和多糖损失率两方面对Sevag法和三氯醋酸法进行比较。对大理百合多糖,提取液∶浓缩液=3∶1(体积比),乙醇加量为4倍体积的浓缩液,Sevag法去除蛋白质为最佳醇沉工艺和脱蛋白方法。     

超声波对醇法大豆浓缩蛋白吸油性的影响

为改善醇法大豆浓缩蛋白的功能特性,采用超声波技术对醇法大豆浓缩蛋白进行物理改性。通过单因素实验,针对吸油性进行研究,得出最佳影响范围,然后进行正交实验并由STATISTIC软件分析,最终得出超声波技术提高醇法大豆浓缩蛋白吸油性最佳工艺条件:固液比为1∶7,功率密度为0.6w/cm2,时间为5min。在此条件下,醇提大豆浓缩蛋白的吸油率可提高97.8%。     

金针菇多糖提取新工艺的优化

对金针菇子实体多糖的提取新工艺进行了系统的优化研究。结果表明 ,子实体多糖提取的最优工艺为 :原料经预处理 ,经 0 .15 %的纤维素酶在 40℃、pH4 5条件下水解 3h ,再用 2 0倍样品重量的水在 10 0℃、pH6 5条件下浸提 1h ,过滤 ,滤液经MWCO5 0 0 0的超滤膜在40℃、0 .2MPa下浓缩至原体积的 1/3后用终体积分数为 70 %的乙醇沉淀 ,经脱蛋白干燥 ,得到纯品金针菇多糖 ,产率为 19 9g/g(干重 ) ,说明用超滤浓缩代替热浓缩是可行的。     

Reliable identification of grapevine rootstock varieties using RAPD PCR on woody samples

Since grapevine ( Vitis spp .) rootstock material is being traded increasingly as disbudded woody material a lack of distinctive morphological features on such material necessitates an alternative and reliable means of identification. Methods described here were developed for rapid and efficient extraction of DNA from woody samples rich in phenolic compounds and polysaccharides, and for subsequent identification of varieties by RAPD PCR. Using these methods, and with the application of only one selected RAPD primer, we were able to differentiate sixteen rootstock varieties, including the seven varieties most commonly used in Germany. Problems commonly encountered with reproducibility of RAPD patterns were avoided by choosing primers with a dinucleotide sequence and a high G/C content that allowed a rather high annealing temperature of 45°C. Methods described here should also be useful for other horticultural crops, especially those with woody tissues rich in phenolic compounds and polysaccharides.     

An internet compendium of analytical methods and spectroscopic information for monomers and additives used in food packaging plastics

An internet website (http://cpf.jrc.it/smt/) has been produced as a means of dissemination of methods of analysis and supporting spectroscopic information on monomers and additives used for food contact materials (principally packaging). The site which is aimed primarily at assisting food control laboratories in the European Union contains analytical information on monomers, starting substances and additives used in the manufacture of plastics materials. A searchable index is provided giving PM and CAS numbers for each of 255 substances. For each substance a data sheet gives regulatory information, chemical structures, physico-chemical information and background information on the use of the substance in particular plastics, and the food packaging applications. For monomers and starting substances (155 compounds) the infra-red and mass spectra are provided, and for additives (100 compounds); additionally proton NMR are available for about 50% of the entries. Where analytical methods have been developed for determining these substances as residual amounts in plastics or as trace amounts in food simulants these methods are also on the website. All information is provided in portable document file (PDF) format which means that high quality copies can be readily printed, using freely available Adobe Acrobat Reader software. The website will in future be maintained and up-dated by the European Commission's Joint Research Centre (JRC) as new substances are authorized for use by the European Commission (DG-ENTR formerly DGIII). Where analytical laboratories (food control or other) require reference substances these can be obtained free-ofcharge from a reference collection housed at the JRC and maintained in conjunction with this website compendium.     

Aroma characterization of various apricot varieties using headspace–solid phase microextraction combined with gas chromatography–mass spectrometry and gas chromatography–olfactometry

The characterization of the aromatic profile of several apricot cultivars with molecular tracers in order to obtain objective data concerning the aromatic quality of this fruit was undertaken using headspace–solid phase microextraction (HS–SPME). Six apricot cultivars were selected according to their organoleptic characteristics: Iranien, Orangered, Goldrich, Hargrand, Rouge du Roussillon and A4025. The aromatic intensity of these varieties measured by HS–SPME–Olfactometry were defined and classified according to the presence and the intensity of grassy, fruity and apricot like notes. In the six varieties, 23 common volatile compounds were identified by HS–SPME–GC–MS. Finally, 10 compounds, ethyl acetate, hexyl acetate, limonene, β-cyclocitral, γ-decalactone, 6-methyl-5-hepten-2-one, linalool, β-ionone, menthone and (E)-hexen-2-al were recognized by HS–SPME–GC–O as responsible of the aromatic notes involved in apricot aroma and considered as molecular tracers of apricot aromatic quality which could be utilized to discriminate apricot varieties.     

Tests of potential functional barriers for laminated multilayer food packages. Part I: Low molecular weight permeants

The advent of the functional barrier concept in food packaging has brought with it a requirement for fast tests of permeation through potential barrier materials. In such tests it would be convenient for both foodstuffs and materials below the functional barrier (sub-barrier materials) to be represented by standard simulants. By means of inverse gas chromatography, liquid paraffin spiked with appropriate permeants was considered as a potential simulant of sub-barrier materials based on polypropylene (PP) or similar polyolefins. Experiments were performed to characterize the kinetics of the permeation of low molecular weight model permeants (octene, toluene and isopropanol) from liquid paraffin, through a surrogate potential functional barrier (25 μm-thick oriented PP) into the food simulants olive oil and 3% (w/v) acetic acid. These permeation results were interpreted in terms of three permeation kinetic models regarding the solubility of a particular model permeant in the post-barrier medium (i.e. the food simulant). The results obtained justify the development and evaluation of liquid sub-barrier simulants that would allow flexible yet rigorous testing of new laminated multilayer packaging materials.     

Analysis of benzo[a]pyrene in spiked fatty foods by second derivative synchronous spectrofluorimetry after microwave-assisted treatment of samples

A simple, rapid and inexpensive method has been developed for the determination of benzo[a     

The development of reference materials for paralytic shellfish poisoning toxins in lyophilized mussel. II: Certification study

This paper describes the second part of a project undertaken to develop certified mussel reference materials for paralytic shellfish poisoning toxins. In the first part two interlaboratory studies were undertaken to investigate the performance of the analytical methodology for several PSP toxins, in particular saxitoxin and decarbamoyl-saxitoxin in lyophilized mussels, and to set criteria for the acceptance of results to be applied during the certification exercise. Fifteen laboratories participated in this certification study and were asked to measure saxitoxin and decarbamoyl-saxitoxin in rehydrated lyophilized mussel material and in a saxitoxin-enriched mussel material. The participants were allowed to use a method of their choice but with an extraction procedure to be strictly followed. The study included extra experiments to verify the detection limits for both saxitoxin and decarbamoyl-saxitoxin. Most participants (13 of 15) were able to meet all the criteria set for the certification study. Results for saxitoxin.2HCl yielded a certified mass fraction of <0.07 mg/kg in the rehydrated lyophilized mussels. Results obtained for decarbamoyl-saxitoxin.2HCl yielded a certified mass fraction of 1.59+/-0.20 mg/kg. The results for saxitoxin.2HCl in enriched blank mussel yielded a certified mass fraction of 0.48 +/- 0.06 mg/kg. These certified reference materials for paralytic shellfish poisoning toxins in lyophilized mussel material are the first available for laboratories to test their method for accuracy and performance.     

Announcing a new international peer-reviewed journal:Food Science and Human Wellness now accepting manuscript submission in English

<正>We are pleased to announce the launch of a new international peer-reviewed journal-Food Science and Human Wellness,ISSN 2213-4530,which is an open access journal,produced and hosted by Elsevier B.V.on behalf of Beijing Academy of Food Sciences.Food Science and Human Wellness is an international peer-reviewed English journal that provides a forum for the dissemination of the     

Chinese CTP Market

According to Printing and Printing Equipment Industries Association of China（PEIAC）＇s statistics to the plate manufucturer in China, in 2013, the actual offset plate production has reached 346 million square meters in China. Among them, the CTP production volume was 245 million square meters, up by 11% than that of last year; the total sales of the CTP plate was 239 million square meters, up by 13%.     

Preparatory Work of Print China 2015 is Going Smoothly

正Held at Guangdong Modern International Exhibition Center,Print China 2015 will cover 7exhibition halls,besides the original Hall No.3,4,5,6,7,the newly built F zone of Hall 3 will be used too.The total area will be140,000 square meters.Hall 3:Offset and large printing equipment,package printing equipment,post press