稻米中普鲁兰酶的纯化与性质的研究

稻米的普鲁兰酶（ＰｕｌｌｕｌａｎａｓｅＥ．Ｃ．３．２．１．４１）用ＤＥＡＥ－纤维素吸附，ＳｅｐｈａｄｅｘＧ－１００凝胶过滤，聚丙烯酰胺盘状凝胶电泳纯化，得到了聚丙烯酰胺凝胶电泳均一的纯酶。纯酶作用的最适温度为５０℃，最适ｐＨ为５．５。以普鲁兰（Ｐｕｌｌｕｌａｎ）为底物酶的米氏常数Ｋｍ为０．０１２５％，以支链淀粉为底物，酶的米氏常数Ｋｍ为２．５％，用ＳｅｐｈａｄｅｘＧ－１００凝胶层析法测得酶的分子量为７００００。     

UASB反应器处理烧碱-蒽醌草浆黑液的污泥颗粒化过程

用两套处理Ｓｏｄａ－ＡＱ化学草浆黑液上流式厌氧污泥床（ＵＡＳＢ）反应器作对比实验，研究处理这种废水的ＵＡＳＢ反应器的污泥颗粒化过程，并达到了１０ｋｇＣＯＤ／ｍ３·ｄ的工作负荷及在此负荷下ＣＯＤ（ｃｒ）４０％～５０％、ＢＯＤ５５６．５％～６３．５％的去除率，去除１ｇＣＯＤ产气量达到５１１ｍｌ，其中ＣＨ４含量为６６．８％。     

张家港市港达染料化工厂供货信息

阳离子染料：红 Ｘ－ＧＲＬ,蓝Ｘ－ＧＲＲＬ,黄 Ｘ－ＳＧＬ,金黄Ｘ－ＧＬ,艳红ＳＧＮ,红Ｘ－ＧＴＬ,翠蓝ＧＢ,桃红ＦＧ,蓝Ｘ－ＢＬ,蓝Ｘ－ＧＲＬ。 进口活性染料：黄 ３ＲＳ,红 ３ＢＳ,红 ＢＳ,红 Ｂ,蓝Ｇ,艳蓝 ＢＢ,艳黄 ＧＬ,．黑Ｂ,．黑 Ｎ。 可拔性染料。阳离子拔色黄３ＲＬ,可拔分散黄ＰＤ－Ｇ,橙 ＰＤ－Ｒ,大红 ＰＤ－ＧＧＳ,蓝 ＰＤ－ＧＤ。藏青 ＦＤ－２ＲＤ,深棕 ＰＤ－ＢＲＳ,黑 ＰＤ－ＲＳ,ＩＰＤ－ＧＳ。 荧光增白剂;腈纶荧光增白剂ＣＨ,腈纶荧光增白剂 ＢＡＣ（瑞士）,棉用荧光增白剂 ＢＴＷ（瑞士…     

亚硫酸氢钠、没食子酸丙酯阻断大鼠体内N－亚硝基脯氨酸的合成

以２４小时尿中Ｎ－亚硝基脯氨酸（ＮＰＲＯ）排泄量为指标，研究亚硫酸氢钠（ＮａＨＳＯ３）和没食于酸丙酯（ＰＧ）阻断大鼠体内Ｎ－亚硝基脯氨酸合成的作用。结果证明１７．５ｍｇＮａＨＳＯ３、５ｍｇＰＧ能有效地阻断４０μｍｏｌ脯氨酸和４０μｍｏｌ亚硝酸钠在大鼠体内合成ＮＰＲＯ，阻断率分别为９９．７８％和９２．９８％。     

L－缬氨酸菌种选育

以硫酸二乙酯（ＤＥＳ）诱变处理黄色短杆菌（Ｂｒｅｖｉｂａｃｔｅｒｉｕｍｆｌａｖｕｍ）ＸＱ５１２２，得到突变株Ｖ３－３６＜Ｌｅｕ￣１、α－ＡＢ￣ｒ、ＡＨＶ￣ｒ），在１０％葡萄糖培养基中可积累２．３％Ｌ－缬氨酸。以亚硝基胍（ＮＴＧ）诱变Ｖ４－１５３，得到一株突变株（Ｌｅｕ￣１、α－ＡＢ￣ｒ、ＡＨＶ￣ｒ、２－ＴＡ￣ｒ），再进行单菌落分离，得到突变株ＺＱ－２，能在培养基中积累Ｌ－缬氨酸４．２％～４．５％，最高达５．５７％．     

内硝基冠醚中间体的合成

研究了作为制备新型内硝基冠醚原料的中间体２－硝基－１，３－苯二酚（ＮＢ）及多甘醇双对甲苯磺酸酯（ＯＥＤＴ）的合成．通过改进ＮＢ的合成条件，使磺化、硝化、水解三步总收率由１２．９％提高到３７．５％；以吡啶为溶剂和ＨＣｌ的束缚剂，控制反应物摩尔比为ＴｓＣｌ∶ＯＥＧ＝２．０６∶１．００，在０℃～５℃反应２ｈ后，加入适量水静置过夜，可明显提高ＯＥＤＴ的收率和纯度．     

气相色谱—质谱法测定蜂王浆及其制品中10—羟基—α—癸烯酸

试样（鲜浆、冻干粉、口服液）经稀盐酸溶液调节ｐＨ为３．０以下,以乙醚萃取,气相色谱法（ＧＣ－ＭＳＤ）测定,外标法定量。方法简便、快速、准确,回收率９７．６％￣９８．９％,相对标准偏差２．０８％￣４．３９％。     

顶青霉木聚糖酶的分离纯化与鉴定

使用硫酸铵分级沉淀、ＳｅｐｈａｄｅｘＧ－２５凝胶色谱脱盐、ＤＥＡＥ－ＳｅｐｈａｄｅｘＡ－２５和ＣＭ－ＳｅｐｈａｄｅｘＣ－５０离子交换色谱等分离纯化技术,从顶青霉（Ｐｅｎｃｉｌｉｕｍｃｏｒｙｌｏｐｈｉｌｕｍ）培养液中分离到３种木聚糖酶组分,分别称为ＰａｒｔＡ、ＰａｒｔＢ和ＰａｒｔＣ．ＰａｒｔＢ和ＰａｒｔＣ经ＳｅｐｈａｃｒｙｌＳ－２００凝胶过滤色谱和ＣｏｎＡ－Ｓｅｐｈａｒｏｓｅ４Ｂ亲和色谱进一步分离纯化,分别得到２个纯木聚糖酶组分．ＰａｒｔＢ经ＳＤＳ－ＰＡＧＥ鉴定为单带,相对分子质量为２４２００;ＰａｒｔＣ经ＳＤＳ－ＰＡＧＥ鉴定基本为单带,相对分子质量为４８３００     

冷库温湿度的微机控制

介绍了冷库温湿度电脑控制仪，该控制仪适用于各种类型冷库的温湿度控制。主要由ＭＣＳ－５１系列单片机、Ａ／Ｄ转换板、输出控制电路、显示电路、报警电路、测温和测湿器件等组成。温湿度控制范围为：温度（－４０±０．５）～（１１±０．５）℃；相对湿度（０％～１００％）±（２％～３％）．几年来的试运行表明，利用该电脑控制仪控制冷库的温湿度，精度高，库内温湿度波动小，提高了冷冻产品的质量，而且能耗明显降低，平均节电８％～１０％．     

高效液相色谱快速测定食品及饲料中抗氧化剂BHT

用高效液相色谱法紫外检测食品和宠物饲料中抗氧化剂ＢＨＴ含量，采用Ｎｏｖａ－ｐａｋＯＤＳ３．９×１５０ｍｍｉ．ｄ．（４μｍ）色谱柱，甲醇：水：３６％乙酸（８５：１０：５）为流动相，紫外检测波长为２８０ｎｍ。宠物饲料的回收率为９３．０－１００．５％，食用油的回收率为９３．５－１１７．０％，变异系数均小于８．３％。本方法快速、简便、灵敏，可用于食品的日常测定     

Reliable identification of grapevine rootstock varieties using RAPD PCR on woody samples

Since grapevine ( Vitis spp .) rootstock material is being traded increasingly as disbudded woody material a lack of distinctive morphological features on such material necessitates an alternative and reliable means of identification. Methods described here were developed for rapid and efficient extraction of DNA from woody samples rich in phenolic compounds and polysaccharides, and for subsequent identification of varieties by RAPD PCR. Using these methods, and with the application of only one selected RAPD primer, we were able to differentiate sixteen rootstock varieties, including the seven varieties most commonly used in Germany. Problems commonly encountered with reproducibility of RAPD patterns were avoided by choosing primers with a dinucleotide sequence and a high G/C content that allowed a rather high annealing temperature of 45°C. Methods described here should also be useful for other horticultural crops, especially those with woody tissues rich in phenolic compounds and polysaccharides.     

An internet compendium of analytical methods and spectroscopic information for monomers and additives used in food packaging plastics

An internet website (http://cpf.jrc.it/smt/) has been produced as a means of dissemination of methods of analysis and supporting spectroscopic information on monomers and additives used for food contact materials (principally packaging). The site which is aimed primarily at assisting food control laboratories in the European Union contains analytical information on monomers, starting substances and additives used in the manufacture of plastics materials. A searchable index is provided giving PM and CAS numbers for each of 255 substances. For each substance a data sheet gives regulatory information, chemical structures, physico-chemical information and background information on the use of the substance in particular plastics, and the food packaging applications. For monomers and starting substances (155 compounds) the infra-red and mass spectra are provided, and for additives (100 compounds); additionally proton NMR are available for about 50% of the entries. Where analytical methods have been developed for determining these substances as residual amounts in plastics or as trace amounts in food simulants these methods are also on the website. All information is provided in portable document file (PDF) format which means that high quality copies can be readily printed, using freely available Adobe Acrobat Reader software. The website will in future be maintained and up-dated by the European Commission's Joint Research Centre (JRC) as new substances are authorized for use by the European Commission (DG-ENTR formerly DGIII). Where analytical laboratories (food control or other) require reference substances these can be obtained free-ofcharge from a reference collection housed at the JRC and maintained in conjunction with this website compendium.     

Aroma characterization of various apricot varieties using headspace–solid phase microextraction combined with gas chromatography–mass spectrometry and gas chromatography–olfactometry

The characterization of the aromatic profile of several apricot cultivars with molecular tracers in order to obtain objective data concerning the aromatic quality of this fruit was undertaken using headspace–solid phase microextraction (HS–SPME). Six apricot cultivars were selected according to their organoleptic characteristics: Iranien, Orangered, Goldrich, Hargrand, Rouge du Roussillon and A4025. The aromatic intensity of these varieties measured by HS–SPME–Olfactometry were defined and classified according to the presence and the intensity of grassy, fruity and apricot like notes. In the six varieties, 23 common volatile compounds were identified by HS–SPME–GC–MS. Finally, 10 compounds, ethyl acetate, hexyl acetate, limonene, β-cyclocitral, γ-decalactone, 6-methyl-5-hepten-2-one, linalool, β-ionone, menthone and (E)-hexen-2-al were recognized by HS–SPME–GC–O as responsible of the aromatic notes involved in apricot aroma and considered as molecular tracers of apricot aromatic quality which could be utilized to discriminate apricot varieties.     

Tests of potential functional barriers for laminated multilayer food packages. Part I: Low molecular weight permeants

The advent of the functional barrier concept in food packaging has brought with it a requirement for fast tests of permeation through potential barrier materials. In such tests it would be convenient for both foodstuffs and materials below the functional barrier (sub-barrier materials) to be represented by standard simulants. By means of inverse gas chromatography, liquid paraffin spiked with appropriate permeants was considered as a potential simulant of sub-barrier materials based on polypropylene (PP) or similar polyolefins. Experiments were performed to characterize the kinetics of the permeation of low molecular weight model permeants (octene, toluene and isopropanol) from liquid paraffin, through a surrogate potential functional barrier (25 μm-thick oriented PP) into the food simulants olive oil and 3% (w/v) acetic acid. These permeation results were interpreted in terms of three permeation kinetic models regarding the solubility of a particular model permeant in the post-barrier medium (i.e. the food simulant). The results obtained justify the development and evaluation of liquid sub-barrier simulants that would allow flexible yet rigorous testing of new laminated multilayer packaging materials.     

Analysis of benzo[a]pyrene in spiked fatty foods by second derivative synchronous spectrofluorimetry after microwave-assisted treatment of samples

A simple, rapid and inexpensive method has been developed for the determination of benzo[a     

The development of reference materials for paralytic shellfish poisoning toxins in lyophilized mussel. II: Certification study

This paper describes the second part of a project undertaken to develop certified mussel reference materials for paralytic shellfish poisoning toxins. In the first part two interlaboratory studies were undertaken to investigate the performance of the analytical methodology for several PSP toxins, in particular saxitoxin and decarbamoyl-saxitoxin in lyophilized mussels, and to set criteria for the acceptance of results to be applied during the certification exercise. Fifteen laboratories participated in this certification study and were asked to measure saxitoxin and decarbamoyl-saxitoxin in rehydrated lyophilized mussel material and in a saxitoxin-enriched mussel material. The participants were allowed to use a method of their choice but with an extraction procedure to be strictly followed. The study included extra experiments to verify the detection limits for both saxitoxin and decarbamoyl-saxitoxin. Most participants (13 of 15) were able to meet all the criteria set for the certification study. Results for saxitoxin.2HCl yielded a certified mass fraction of <0.07 mg/kg in the rehydrated lyophilized mussels. Results obtained for decarbamoyl-saxitoxin.2HCl yielded a certified mass fraction of 1.59+/-0.20 mg/kg. The results for saxitoxin.2HCl in enriched blank mussel yielded a certified mass fraction of 0.48 +/- 0.06 mg/kg. These certified reference materials for paralytic shellfish poisoning toxins in lyophilized mussel material are the first available for laboratories to test their method for accuracy and performance.     

Announcing a new international peer-reviewed journal:Food Science and Human Wellness now accepting manuscript submission in English

<正>We are pleased to announce the launch of a new international peer-reviewed journal-Food Science and Human Wellness,ISSN 2213-4530,which is an open access journal,produced and hosted by Elsevier B.V.on behalf of Beijing Academy of Food Sciences.Food Science and Human Wellness is an international peer-reviewed English journal that provides a forum for the dissemination of the     

Chinese CTP Market

According to Printing and Printing Equipment Industries Association of China（PEIAC）＇s statistics to the plate manufucturer in China, in 2013, the actual offset plate production has reached 346 million square meters in China. Among them, the CTP production volume was 245 million square meters, up by 11% than that of last year; the total sales of the CTP plate was 239 million square meters, up by 13%.     

Preparatory Work of Print China 2015 is Going Smoothly

正Held at Guangdong Modern International Exhibition Center,Print China 2015 will cover 7exhibition halls,besides the original Hall No.3,4,5,6,7,the newly built F zone of Hall 3 will be used too.The total area will be140,000 square meters.Hall 3:Offset and large printing equipment,package printing equipment,post press