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Ait-Ouazzou A Mañas P Condón S Pagán R García-Gonzalo D 《International journal of food microbiology》2012,153(3):358-364
This investigation aimed to determine the role of general stress-response alternative sigma factors σS (RpoS) and σB (SigB) in heat resistance and the occurrence of sublethal injuries in cell envelopes of stationary-phase Escherichia coli BJ4 and Listeria monocytogenes EGD-e cells, respectively, as a function of treatment medium pH. Given that microbial death followed first-order inactivation kinetics (R2 > 0.95) the traditional DT and z values were used to describe the heat inactivation kinetics.Influence of rpoS deletion was constant at every treatment temperature and pH, making a ΔrpoS deletion mutant strain approximately 5.5 times more heat sensitive than its parental strain for every studied condition. Furthermore, the influence of the pH of the treatment medium on the reduction of the heat resistance of E. coli was also constant and independent of the treatment temperature (average z value = 4.9 °C) in both parental and mutant strains.L. monocytogenes EGD-e z values obtained at pH 7.0 and 5.5 were not significantly different (p > 0.05) in either parental or the ?sigB deletion mutant strains (average z value = 4.8 °C). Nevertheless, at pH 4.0 the z value was higher (z = 8.4 °C), indicating that heat resistance of both L. monocytogenes strains was less dependent on temperature at pH 4.0. At both pH 5.5 and 7.0 the influence of sigB deletion was constant and independent of the treatment temperature, decreasing L. monocytogenes heat resistance approximately 2.5 times. In contrast, the absence of sigB did not decrease the heat resistance of L. monocytogenes at pH 4.0.The role of RpoS in protecting cell envelopes was more important in E. coli (4 times) than SigB in L. monocytogenes (1.5 times). Moreover, the role of σS in increasing heat resistance seems more relevant in enhancing the intrinsic resilience of the cytoplasmic membrane, and to a lesser extent, outer membrane resilience.Knowledge of environmental conditions related to the activation of alternative sigma factors σS and σB and their effects on heat resistance would help us to avoid and/or identify situations that increase bacterial stress resistance. Therefore, more efficient food preservation processes might be designed. 相似文献
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由瑞士Staubli Sargans公司所产LX3201新型高速提花机,能配置14336针。该机适合应用于平面织物,尤其是丝绸和室内装饰品的高速生产。 相似文献
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前言纺织纤维分为天然纤维和人造纤维。在这两大类纤维中,最重要的纤维是棉花、羊毛和亚麻。人造纤维又可进一步分为再生纤维、合成纤维和无机纤维。作为纺织原料的最重要的再生纤维是用粘胶法生产的粘胶和莫代尔再生纤维素纤维,而最重要的合成纤维是聚酯纤维、聚丙烯腈纤维和聚酰胺纤维。这一分类法示于表1。 相似文献
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在土耳其伊斯坦布尔举行的ITM 2006上,瑞士Stubli公司展示了两款新型电子提花机--LX 1602和LX 3202(图1).两种机型都是设计用于高速高产织造普通织物和毛圈织物,是在LX 1600/3201机型的基础上开发的.两款新机型保留了原有的概念并接纳了许多改进,具有更好的性价比. 相似文献
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给出了求函数方程f(α b)=f(α) f(b)的所有连续解的一种方法,并分析了这种方法形成的直观背景. 相似文献
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Giuseppe G. G. Manzardo Sandra Kürsteiner-Laube Daniel Perrin 《European Food Research and Technology》1996,203(6):501-506
In diethyl ether extracts from celeriac (Apium graveolens L. var.rapaceum) all four stereoisomers of (3a–7a)-cis-3-butylhexahydrophthalide were found to be present. The analyses were carried out by means of GC, enantioselective GC and GC-MS. The assignment of the relative configuration of the diastereomers3 and4 was accomplished by NOE difference spectroscopy. 相似文献
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设计说明:S12书桌使用美国白橡木制作,圆润别致的外形加上木材的天然纹理是它最主要的特点,此外,为了方便运输、实现平板包装、桌腿是可以拆卸的,在构造上结合了榫卯与五金各自的优点。产品尺寸为长120cm×宽60cm×高75cm.SPACE推荐:这款简洁优雅的书桌,以最简单的表现手法展现出术质自身最天然的魅力。可拆卸的设计便于运输、也体现了环保的概念。价格:1600元/件售卖地点:http://smartwood.taobao.com/ 相似文献
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稻谷中苯并(a)芘的测定 总被引:8,自引:0,他引:8
将粉碎后的稻谷样品置于脂肪提取器中,采用石油醚回流提取8h,提取液用中性氧化铝净化,KD浓缩器浓缩。采用液相色谱对稻谷中苯并芘的含量进行测定,分离柱ODCS-C18;流动相为85%,甲醇和15%水,流速为1ml/min;荧光检测器检测。 相似文献
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我厂新购进一台江苏如皋印刷机械厂生产的J2101S(2)型胶印机。在使用过程中,该机经常出现点动变连动;清洗胶辊时,不得不用手搬动飞轮进行擦洗,给操作人员带来许多麻烦。究其原因,是因电气线路存在一定的缺陷: 相似文献
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1982年4月下旬,日本栗田化学研究所在上海第一丝绸印染厂进行技术交流,交流的题目是KR—TEX(S)重氮感光胶。 日方首先介绍了栗田化学研究所的简况。该所是专门研究生产重氮感光胶的商社研究所,重氮感光胶KR-TEX是该所近两年中最新研究生产的商品,用它制作印花网版和印刷网版,具有无味、无毒、制版精细,使用方便的优点,尤其与重铬酸铵相比没有三废之患;但在显影时有显影困难之弊。 相似文献