The recombinant human dentin matrix protein 1-coated titanium and its effect on the attachment, proliferation and ALP activity of MG63 cells

The aim of the present work was to design a bio-interactive implant surface by coating recombinant human dentin matrix protein 1 (hDMP1) onto titanium and to investigate the biological function of this material. Firstly, the plasmid containing the hDMP1 cDNA was constructed and hDMP1 was expressed, purified and characterized. Then, hDMP1 was coated onto the surface of Ti substrates via a biochemical technique and the procedure was divided into three steps: in the beginning, titanium was treated by regular polishing and denoted as Cp-Ti; then, Cp-Ti received alkaline and water treatment and was nominated as AW-Ti; finally, AW-Ti was coated with hDMP1 and referred to as hDMP1-Ti. The inserts of hDMP1 genes were detected by enzyme digestion as well as gel electrophoresis, and the complete nucleotide sequence of hDMP1 was tested. The purified recombinant hDMP1 was electrophoresed on a 10?% SDS-PAGE gel. Cp-Ti, AW-Ti and hDMP1-Ti were characterized by X-ray photoelectron spectroscope and water contact angles tests. The biological activity of MG63 cells cultured in the three groups was investigated by the cell attachment, proliferation and alkaline phosphatase activity assays. The results show that hDMP1 was successfully constructed and coated onto the titanium surface, and hDMP1-Ti had higher hydrophilicity than Cp-Ti. Compared with Cp-Ti and AW-Ti, hDMP1-Ti showed better in vitro bioactivity.     

Effect of anodization on corrosion behaviour and biocompatibility of Cp-titanium in simulated body fluid

The objective of this investigation is to study the effectiveness of anodized surface of commercial purity titanium (Cp-Ti) on its corrosion behaviour in simulated body fluid (SBF) and proliferation of osteoblast cells on it, to assess its potentiality as a process of surface modification in enhancing corrosion resistance and osseointegration of dental implants. Highly ordered nano-porous oxide layer, with nano-sized pores, is developed on the surface of Cp-Ti through electrochemical anodization in the electrolyte of aqueous solution of 0·5% HF at 15 V for 30 min at 24 °C. The nano-porous feature of the anodized surface is characterized by field-emission scanning electron microscope (FESEM). Pores of some anodized samples are sealed by exposing the anodized surface in boiling water. Corrosion behaviour of the anodized specimen is studied in Ringer’s solution at 30 ± 2 °C, using electrochemical impedance and cyclic polarization technique. Biocompatibility of the anodized surface is accessed using MG63 osteoblast cells. Both corrosion as well as pitting resistance of Cp-Ti in simulated body fluid are found to be highest in the anodized and sealed condition and followed in decreasing order by those of anodized and unanodized ones. Significantly higher MG63 osteoblast cell proliferations are found on the anodized surface than that on the unanodized one. Anodized Cp-Ti develops nano-size surface pores, like that of natural bone. It enhances corrosion and pitting resistance and also the process of osteoblast cell proliferation on Cp-Ti.     

Electrochemical behavior of nano and femtosecond laser textured titanium alloy for implant surface modification

In this study, the electrochemical behavior of nano and femtosecond laser textured titanium alloy for implant surface modification has been researched using the potentiostat equipment. Cp-Ti and Ti-6Al-4V alloy, located on X-Y motorized stage, were irradiated using femtosecond laser. The corrosion properties were examined by a potentiodynamic and AC impedance test.     

非连续增强金属基复合材料剧烈塑性变形行为研究进展

综述了非连续增强金属基复合材料剧烈塑性变形(SPD)行为的研究进展,系统阐述了等径弯曲通道变形(ECAP)、高压扭转(HPT)、多向锻造(MF)、累积叠轧(ARB)和循环挤压压缩(CEC)5种SPD的加工原理和方法。集中介绍了这些方法在铝基、镁基、铜基和钛基等金属基复合材料方面应用的研究进展。重点介绍了金属基复合材料SPD的微观组织演化和变形力学行为,详细阐明了金属基复合材料SPD机制以及超细晶形成机理,指出了金属基复合材料在SPD中存在的深层次问题及发展趋势,展望了利用SPD方法制备超细晶非连续增强金属基复合材料的应用前景。     

Strength and fatigue properties enhancement in ultrafine-grained Ti produced by severe plastic deformation

Severe plastic deformation (SPD) of titanium creates an ultrafine-grained (UFG) microstructure which results in significantly enhanced mechanical properties, including increasing the high cycle fatigue strength. This work addresses the challenge of maintaining the high level of properties as SPD processing techniques are evolved from methods suitable for producing laboratory scale samples to methods suitable for commercial scale production of titanium semi-products. Various ways to optimize the strength and fatigue endurance limit in long-length Grade 4 titanium rod processed by equal channel angular pressing (ECAP) with subsequent thermal mechanical treatments are considered in this paper. Low-temperature annealing of rods is found to increase the fatigue limit, simultaneously enhancing UFG titanium strength and ductility. The UFG structure in titanium provides an optimum combination of properties when its microstructure includes mostly equiaxed grains with high-angle boundaries, the volume fraction of which is no less than 50%.     

In vitro fibroblast response to ultra fine grained titanium produced by a severe plastic deformation process

The in vitro response of the mouse fibroblast cell line 3T3 on the surface of ultrafine grained titanium [produced by a severe plastic deformation (SPD) process] has been studied in this work. SPD Ti showed much higher strength than the coarse grained Ti and equivalent to that of Ti–6Al–4V alloy. Better cell proliferation was observed on SPD Ti compared to conventional Ti and Ti–6Al–4V alloy. This could be attributed to the increased surface free energy by reduction in the grain size and possibly the presence of a large number of nano size grooves at the triple point junctions in SPD Ti sample. There was no significant difference in the results of cytotoxicity tests of fine and coarse grained materials.     

应用等通道转角挤压(ECAP)技术制备超细晶钛合金

等通道转角挤压(equal channel angular pressing,ECAP)是一种强塑性变形技术,能有效细化材料的微观组织,提高材料性能,改善难变形材料的成形性.简述了ECAP技术制备超细晶钛合金的原理和技术现状,分析了不同工艺参数对钛合金ECAP变形过程和材料性能的影响以及晶粒细化的微观机制.     

塑性变形诱发表面自纳米化的研究及其应用

详细介绍了强烈塑性变形诱发表面自纳米化的研究进展,即形变诱发表面自纳米化的方法与设备、表面自纳米化层的结构及其表征、形变诱发表面自纳米化的机理及其相关应用,并展望了形变诱发表面自纳米化的研究与应用.     

Enhancement of mechanical properties of biocompatible Ti–45Nb alloy by hydrostatic extrusion

β-Type titanium alloys are promising materials for orthopaedic implants due to their relatively low Young’s modulus and excellent biocompatibility. However, their strength is lower than those of α- or α + β-type titanium alloys. Grain refinement by severe plastic deformation (SPD) techniques provides a unique opportunity to enhance mechanical properties to prolong the lifetime of orthopaedic implants without changing their chemical composition. In this study, β-type Ti–45Nb (wt%) biomedical alloy in the form of 30 mm rod was subjected to hydrostatic extrusion (HE) to refine the microstructure and improve its mechanical properties. HE processing was carried out at room temperature without intermediate annealing in a multi-step process, up to an accumulative true strain of 3.5. Significant microstructure refinement from a coarse-grained region to an ultrafine-grained one was observed by optical and transmission electron microscopy. Vickers hardness measurements (HV_0.2) demonstrated that the strength of the alloy increased from about 150 to 210 HV_0.2. Nevertheless, the measurements of Young’s modulus by nanoindentation showed no significant changes. This finding is substantiated by X-ray diffraction analyses which did not exhibit any phase transformation out of the bcc phase being present still before processing by HE. These results thus indicate that HE is a promising SPD method to obtain significant grain refinement and enhance strength of β-type Ti–45Nb alloy without changing its low Young’s modulus, being one prerequisite for biomedical application.     

Synergic effects of grain refinement and precipitation strengthening

The article describes the combined effects of grain size and second phase particles on mechanical properties of CuCrZr alloy subjected to SPD processing and ageing in two sequences: (i) SPD processing followed by ageing and (ii) SPD processing of samples aged prior to deformation. It was revealed that each of these strengthening mechanisms acting alone gives a significant increase in mechanical strength (5 and 10 times in the case of ageing and SPD processing, respectively). However, it has been found in the present study that the strength of samples subjected to grain refinement and precipitation hardening is not a direct sum of the strengthening brought about by these two strengthening mechanisms acting alone. This finding is discussed in terms of the inter-dependence of grain size–particle strengthening in SPD nano-metals.     

大塑性变形制备超细晶/纳米晶Al-Mg铝合金的研究进展

近年来,大塑性变形(SPD)制备具有先进结构和功能的超细晶和纳米晶Al-Mg铝合金的研究取得了很大进展。SPD后,合金的晶粒显著细化、位错密度提高及有非平衡晶界和晶界偏析形成,这些微观结构导致合金的强度、硬度大幅提高。然而,SPD合金的塑性普遍较低。综述了SPD制备的Al-Mg铝合金在结构和性能方面的一些最新研究成果。     

Evolution Behaviors of Oxides in Severely Plastic Deformed Region of AISI 52100 Steel during Dry Sliding Wear

Under dry sliding wear, the evolution of oxides in severely plastic deformed(SPD) regions of metals has a great impact on the wear behaviors. To study the evolution behaviors of oxides in the SPD region, an SPD region was prefabricated on the surface of AISI 52100 steel by supersonic ?ne particle bombarding(SFPB) treatment. Dry sliding wear tests were carried out on both of the SFPB-treated and original samples.Wear volume loss of the SPBF-treated samples were compared with those of the original samples at different loads. Microstructure, element composition and oxides distribution in the SPD region were characterized by scanning electron microscopy(SEM), transmission electron microscopy(TEM) and an electron probe microanalysis(EPMA). The results show that the evolution behaviors of the oxides in the SPD region change signi?cantly with the load. Under low loads, oxides are usually formed on the contact surface. It inhibits adhesive wear on the steel. However, under high loads, oxides are apt to distribute along the cracks in the subsurface layer. The internal oxidation along the cracks can accelerate the cracks propagation, resulting in severe delamination wear on the steel.     

Fast Near-duplicate Image Detection in Riemannian Space by A Novel Hashing Scheme

There is a steep increase in data encoded as symmetric positive definite (SPD) matrix in the past decade. The set of SPD matrices forms a Riemannian manifold that constitutes a half convex cone in the vector space of matrices, which we sometimes call SPD manifold. One of the fundamental problems in the application of SPD manifold is to find the nearest neighbor of a queried SPD matrix. Hashing is a popular method that can be used for the nearest neighbor search. However, hashing cannot be directly applied to SPD manifold due to its non-Euclidean intrinsic geometry. Inspired by the idea of kernel trick, a new hashing scheme for SPD manifold by random projection and quantization in expanded data space is proposed in this paper. Experimental results in large scale near-duplicate image detection show the effectiveness and efficiency of the proposed method     

Opto-electronic properties of SnO2 layers obtained by SPD and ECD techniques

The paper presents a comparative approach concerning the properties of SnO₂ thin layers obtained via spray pyrolysis deposition (SPD) and electro-chemical deposition (ECD). The influences of crystalline structure (X-ray diffraction), morphology (atomic force microscopy, contact angle) on the electric (electrical conductivity) properties of the layers were studied. The SPD samples present a porous morphology with high surface energy compared with ECD samples characterized by a dense morphology. The photocatalytic efficiency of the samples was tested in the photodegradation of methylene blue and the higher values (57%) correspond to SPD samples.     

Recent development in grain refinement by hydrostatic extrusion

Hydrostatic extrusion is an efficient method of grain refinement to the nanometer scale in metallic materials. The paper shows that it can be used directly to obtain a mean grain size smaller than 100 nm with a significant fraction of high angle grain boundaries in aluminum alloys, titanium, and iron. It is also demonstrated that grain size reduction to this level in some other materials, e.g., nickel, requires a combination of hydrostatic extrusion (HE), as the final operation, after some other methods of severe plastic deformation (SPD). Grain refinement in metallic materials by HE has a significant effect on their properties with a significant increase in mechanical strength and improvement of wear and corrosion resistance while maintaining an acceptable level of plasticity.     

Review of principles and methods of severe plastic deformation for producing ultrafine-grained tubes

Severe plastic deformation (SPD) is known to be the best method for producing bulk ultrafine-grained and nanostructured materials with excellent properties. Different SPD methods were developed that are suitable for sheet and bulk solid materials. During the past decade, efforts have been made to create effective SPD processes suitable for producing cylindrical tubes. In this paper, we review SPD processes intended to produce ultrafine-grained and nanostructured tubes, and their effects on material properties. The paper will focus on introduction of the tube SPD processes, and then comparison of them based on their advantages and disadvantages from the viewpoints of processing and properties.     

Hydrogen evolution from a-C:H films

Diamond-like amorphous carbon films with high hardness, high electrical resistivity, good optical transparency into the infra-red, and good adherence to their substrates may be produced by many methods, most of which result in the incorporation of 20–40 at% hydrogen. These a-C:H films are similar to a-Si:H films and the present work contributes additional evidence for this similarity. a-C:H films were deposited on glass or NaCl-coated glass in a capacitively coupled rf discharge in CH₄. The nature of the films depends upon the specific power density (SPD): polymeric for SPD < 10W cm^?2 torr^?1, diamond-like for SPD between 10 and 20, and graphitic for SPD>25. The amount of chemisorbed hydrogen in the films, as determined by differential scanned calorimetry (DSC), also depends on the SPD (and concomitant negative self-bias of the substrate), decreasing by a factor of six as the SPD increases from 10 to 20 and the negative self-bias increases form 400–650V. Additionally, the exothermic DSC peak sometimes consists of two or more partially resolved overlapping peaks.     

状态价格密度的局部多项式估计及其在VaR中的应用(英文)

本文应用局部多项式拟合方法来估计金融资产价格中隐含的状态价格密度(SPD),在较弱的条件下证明了SPD的估计是相合的。然后基于SPD我们提出了包含经济价值的VaR。从这个意义上讲它比传统的VaR更合理。最后针对Black-Scholes模型进行了数值模拟和失败率检验以评价本文方法的好坏。     

Effect of strain on “hardening by annealing and softening by deformation” phenomena in ultra-fine grained aluminum

Ultra-fine grained (UFG) metals fabricated by severe plastic deformation (SPD) sometimes exhibit peculiar mechanical properties. For example, the “hardening by annealing and softening by deformation” was reported in UFG aluminum, which was totally opposite to the behaviors of conventionally coarse-grained materials. In this study, the effect of SPD strain on the peculiar phenomena was investigated. The UFG aluminum was fabricated by various cycles of the accumulative roll-bonding (ARB) process with lubrication at ambient temperature. The specimen ARB-processed by ten cycles certainly showed the peculiar phenomena. On the other hand, the 6-cycle specimen did not show the phenomena but was softened by annealing and hardened by deformation normally. From the results of microstructural characterization, it was suggested that the difference in the change of the mechanical property during annealing and deformation between 6-cycle and 10-cycle specimens was caused by the difference in the grain size and/or the texture components, which depended on the SPD strain.     

Severe plastic deformation processes for thin samples

Among the known severe plastic deformation (SPD) techniques, one particular group can be defined as SPD processing of thin samples. Their distinctive feature is that one of the sample dimensions, namely the thickness, is much smaller than the other two dimensions. Examples include High Pressure Torsion and two recently developed techniques: the Cone–Cone Method and the High Pressure Tube Twisting. The mentioned group of SPD processes involve frictional forces acting on the large surfaces and a high hydrostatic pressure within the deformation zone. These techniques are particularly suited for microforming of metals. In this article, we outline the commonalities between these three techniques. The microstructure of copper samples deformed by all the three processes is presented and compared with those obtained by equal-channel angular pressing as a reference bulk forming SPD technique.