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Liz Whitley 《International Journal of Dairy Technology》2013,66(2):303-304
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Phenyl‐γ‐valerolactones,flavan‐3‐ol colonic metabolites,protect brown adipocytes from oxidative stress without affecting their differentiation or function
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Laura Mele Stefania Carobbio Nicoletta Brindani Claudio Curti Sergio Rodriguez‐Cuenca Guillaume Bidault Pedro Mena Ilaria Zanotti Michele Vacca Antonio Vidal‐Puig Daniele Del Rio 《Molecular nutrition & food research》2017,61(9)
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Fausto Bruno Dos Reis Almeida Laurine Lacerda Pigosso André Ricardo de Lima Damásio Valdirene Neves Monteiro Célia Maria de Almeida Soares Roberto Nascimento Silva Maria Cristina Roque‐Barreira 《Yeast (Chichester, England)》2014,31(1):1-11
The cell wall of Paracoccidioides brasiliensis, which consists of a network of polysaccharides and glycoproteins, is essential for fungal pathogenesis. We have previously reported that N‐glycosylation of proteins such as N‐acetyl‐β‐d ‐glucosaminidase is required for the growth and morphogenesis of P. brasiliensis. In the present study, we investigated the influence of tunycamicin (TM)‐mediated inhibition of N‐linked glycosylation on α‐ and β‐(1,3)‐glucanases and on α‐(1,4)‐amylase in P. brasiliensis yeast and mycelium cells. The addition of 15 µg/ml TM to the fungal cultures did not interfere with either α‐ or β‐(1,3)‐glucanase production and secretion. Moreover, incubation with TM did not alter α‐ and β‐(1,3)‐glucanase activity in yeast and mycelium cell extracts. In contrast, α‐(1,4)‐amylase activity was significantly reduced in underglycosylated yeast and mycelium extracts after exposure to TM. In spite of its importance for fungal growth and morphogenesis, N‐glycosylation was not required for glucanase activities. This is surprising because these activities are directed to wall components that are crucial for fungal morphogenesis. On the other hand, N‐glycans were essential for α‐(1,4)‐amylase activity involved in the production of malto‐oligosaccharides that act as primer molecules for the biosynthesis of α‐(1,3)‐glucan. Our results suggest that reduced fungal α‐(1,4)‐amylase activity affects cell wall composition and may account for the impaired growth of underglycosylated yeast and mycelium cells. © 2013 The Authors. Yeast published by John Wiley & Sons Ltd. 相似文献
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