灵芝液态发酵有效产物体外抗氧化活性的研究

在灵芝发酵基质中添加银杏叶、薏苡仁后,测定其发酵产物对Fenton反应生成的羟自由基和邻苯三酚自氧化产生的超氧阴离子的清除作用以及还原力。结果表明,清除羟自由基的半抑制率分别为灵芝90μL,添加银杏叶后为86μL,添加薏苡仁后为77μL。清除超氧阴离子的半抑制率分别为灵芝1.7 mL、添加银杏叶后为1.62 mL,添加薏苡仁后为1.5 mL,在100μL时,三者的还原力分别是:灵芝为0.076,添加薏苡仁后为0.084,添加银杏叶后为0.102,表明在清除自由基和还原力方面,灵芝对薏苡仁和银杏叶发酵后比灵芝单独发酵的效果要好,薏苡仁和银杏叶对灵芝体外清除自由基和还原力方面的效果具有强化作用。     

酶法浸提枸杞的研究

通过对外源酶酶解枸杞效果的研究，结果表明：外源酶能显著提高枸杞浸提液中可溶性固形物和营养成分的含量，其单一纤维素酶和木瓜蛋白酶浸提枸杞的适宜pH值分别是5．0～5．5和pH值6．0，适宜的酶质量浓度分别为0．20g／dL和0．40～0．60g／dL，温度分别为55．C和65℃，适宜时间都为50-60min；两种酶组合浸提枸杞的较适pH值、温度、时间和酶组合分别为pH值5．5～6．0，55-60℃．50min和0．20g／dL纤维素酶＋0．40g／dL木瓜蛋白酶。     

罗非鱼鱼皮多肽(TSP-I)抗氧化活性的研究

利用化学发光法检测了罗非鱼鱼皮多肽（TSP—I）对超氧阴离子（O2·^-）自由基的清除能力，利用分光光度法检测其清除二苯代苦味酰自由基（DPPH·）的能力，采用Na2S2O3-I2滴定法测定了其抗油脂氧化的能力。实验发现：罗非鱼鱼皮多肽对二苯代苦味酰自由基的抑制作用强于对超氧阴离子自由基的抑制作用，其清除两种自由基50％所对应的质量浓度（IC50）分别为：13，96mg／mL和16，79mg／mL。添加罗非鱼鱼皮多肽对油脂具有良好的抗氧化作用，其中以添加1．00％的鱼皮多肽抗氧化效果最佳，另外Vc对鱼皮多肽具有协同抗氧化作用，效果优于单独添加0．05％和0．20％鱼皮多肽。     

野生薯蓣膳食纤维的提取及对自由基的清除作用

以薯蓣粉为材料,设计4因素3水平的正交试验,酶法提取膳食纤维。结果表明：0．7％混合酶（晓一淀粉酶与糖化酶质量比为6：1）,处理温度60℃,处理时间80rain,0．5％蛋白酶在60℃处理60min,对淀粉和蛋白质的脱除较彻底。以体外试验研究不同质量浓度（20-140mg／mL）的膳食纤维鲜样和可溶性淀粉对自由基的清除效果,设计3种不同的反应体系：1,1-二苯基-2-苦味肼基自由基（DPPH）、超氧阴离子自由基（O2＋）、羟自由基（·OH）。结果表明：薯蓣膳食纤维对3种自由基都有一定的清除能力,平均消除率分别为53．23％、31．67％、30．38％。对3种自由基清除能力大小顺序为：DPPH自由基〉超氧阴离子自由基〉羟自由基。另外,可溶性淀粉对3种自由基也有轻微的清除能力,其平均清除率分别为2．81％、6．98％、6．48％。     

灵芝发酵枸杞实现多糖复合初步研究

灵芝多糖和枸杞多糖均具有良好的生理活性功能。提取灵芝多糖和枸杞多糖的复合物清除羟自由基活性可以达到53.11%,优于两种多糖单独作用效果。利用灵芝菌株发酵枸杞汁,体系中总糖含量可以达到20g/L以上,发酵液稀释10倍清除羟自由基活性可以达到56.65%,高于灵芝发酵液和枸杞法清除率。初步说明可以通过灵芝发酵实现灵芝多糖和枸杞多糖的复合,为发挥多糖功效提供新的途径。     

油菜蜂花粉酶解物抗氧化性能的研究

对油菜蜂花粉酶解物和水提物进行了体外抗氧化实验。结果表明,花粉酶解物具有良好的抗氧化功能,在50mg/ml浓度下对超氧阴离子自由基、羟自由基和脂质过氧化的抑制率分别达到87．4%、61．2%和95．9%。比较酶解物和水提物抗氧化性能可知,酶解物清除羟自由基和超氧阴离子自由基的能力明显高于水提物,在50mg／ml时,对羟自由基的抑制率比水提物高约30%,清除超氧阴离子自由基的能力高约20%,而抑制脂质过氧化的能力与水提物之间无明显差异。     

蒲公英花中总酚酸和总黄酮含量测定及其抗氧化性能研究

测定蒲公英花75%乙醇提取液中总酚酸和总黄酮含量,并测定该提取液对超氧阴离子自由基、羟自由基和油脂自氧化的清除作用。结果表明：蒲公英花中总酚酸平均含量为4.09%,总黄酮平均含量为3.65%。提取液对超氧阴离子自由基、羟自由基和油脂自氧化均有较好的清除效果,并在供试质量浓度范围内呈现量效关系。对超氧阴离子自由基的半数抑制率IC50为96.1μg/mL,对羟自由基的IC50为7.7μg/mL。蒲公英花提取液可使花生油的过氧化值(POV)显著降低,其效果与在花生油中添加0.10%的VC相当。     

莲子心乙醇提取液体外抗氧化活性的研究

研究了莲子心乙醇提取液对氧自由基的清除作用及其对脂质过氧化反应（LPO）的抑制作用。结果表明：莲子心中总黄酮含量为20．021mg／g。莲子心乙醇提取液对Fenton体系产生的羟自由基和邻苯三酚自氧化产生的超氧阴离子均有清除作用,半数清除量（IC50）分别为0．195g／ml和0．461g／ml;对小鼠肝、肾匀浆自发性过氧化反应有较好的抑制作用,IC50分别为3．996mg／ml和4．527mg／ml。     

枸杞对红酸汤品质及风味的影响

为探究枸杞对红酸汤品质及风味的影响,于红酸汤中添加0％、1％、2％、3％、4％、5％、6％的枸杞浆,28℃恒温发酵5 d,测定枸杞红酸汤中亚硝酸盐、有机酸含量及羟自由基(·OH-)、超氧阴离子自由基(O2-·)清除能力的变化,并利用模糊数学法进行感官评价.结果表明:随着枸杞添加量的增加,红酸汤中亚硝酸盐含量呈现降低趋势...     

莼菜嫩叶胞内多糖体外抗氧化作用研究

采用水和碱两种溶剂提取脱胶莼菜嫩叶胞内多糖,并研究莼菜胞内多糖的体外抗氧化活性。结果表明：两种多糖具有较强的体外还原能力和清除羟自由基能力,与多糖浓度呈正相关,但水溶性多糖还原能力和清除羟自由基能力均强于碱溶性多糖;两种多糖清除体外超氧阴离子自由基的最适浓度分别为2．0mg／ml和5．0mg／ml。     

Reliable identification of grapevine rootstock varieties using RAPD PCR on woody samples

Since grapevine ( Vitis spp .) rootstock material is being traded increasingly as disbudded woody material a lack of distinctive morphological features on such material necessitates an alternative and reliable means of identification. Methods described here were developed for rapid and efficient extraction of DNA from woody samples rich in phenolic compounds and polysaccharides, and for subsequent identification of varieties by RAPD PCR. Using these methods, and with the application of only one selected RAPD primer, we were able to differentiate sixteen rootstock varieties, including the seven varieties most commonly used in Germany. Problems commonly encountered with reproducibility of RAPD patterns were avoided by choosing primers with a dinucleotide sequence and a high G/C content that allowed a rather high annealing temperature of 45°C. Methods described here should also be useful for other horticultural crops, especially those with woody tissues rich in phenolic compounds and polysaccharides.     

An internet compendium of analytical methods and spectroscopic information for monomers and additives used in food packaging plastics

An internet website (http://cpf.jrc.it/smt/) has been produced as a means of dissemination of methods of analysis and supporting spectroscopic information on monomers and additives used for food contact materials (principally packaging). The site which is aimed primarily at assisting food control laboratories in the European Union contains analytical information on monomers, starting substances and additives used in the manufacture of plastics materials. A searchable index is provided giving PM and CAS numbers for each of 255 substances. For each substance a data sheet gives regulatory information, chemical structures, physico-chemical information and background information on the use of the substance in particular plastics, and the food packaging applications. For monomers and starting substances (155 compounds) the infra-red and mass spectra are provided, and for additives (100 compounds); additionally proton NMR are available for about 50% of the entries. Where analytical methods have been developed for determining these substances as residual amounts in plastics or as trace amounts in food simulants these methods are also on the website. All information is provided in portable document file (PDF) format which means that high quality copies can be readily printed, using freely available Adobe Acrobat Reader software. The website will in future be maintained and up-dated by the European Commission's Joint Research Centre (JRC) as new substances are authorized for use by the European Commission (DG-ENTR formerly DGIII). Where analytical laboratories (food control or other) require reference substances these can be obtained free-ofcharge from a reference collection housed at the JRC and maintained in conjunction with this website compendium.     

Aroma characterization of various apricot varieties using headspace–solid phase microextraction combined with gas chromatography–mass spectrometry and gas chromatography–olfactometry

The characterization of the aromatic profile of several apricot cultivars with molecular tracers in order to obtain objective data concerning the aromatic quality of this fruit was undertaken using headspace–solid phase microextraction (HS–SPME). Six apricot cultivars were selected according to their organoleptic characteristics: Iranien, Orangered, Goldrich, Hargrand, Rouge du Roussillon and A4025. The aromatic intensity of these varieties measured by HS–SPME–Olfactometry were defined and classified according to the presence and the intensity of grassy, fruity and apricot like notes. In the six varieties, 23 common volatile compounds were identified by HS–SPME–GC–MS. Finally, 10 compounds, ethyl acetate, hexyl acetate, limonene, β-cyclocitral, γ-decalactone, 6-methyl-5-hepten-2-one, linalool, β-ionone, menthone and (E)-hexen-2-al were recognized by HS–SPME–GC–O as responsible of the aromatic notes involved in apricot aroma and considered as molecular tracers of apricot aromatic quality which could be utilized to discriminate apricot varieties.     

Tests of potential functional barriers for laminated multilayer food packages. Part I: Low molecular weight permeants

The advent of the functional barrier concept in food packaging has brought with it a requirement for fast tests of permeation through potential barrier materials. In such tests it would be convenient for both foodstuffs and materials below the functional barrier (sub-barrier materials) to be represented by standard simulants. By means of inverse gas chromatography, liquid paraffin spiked with appropriate permeants was considered as a potential simulant of sub-barrier materials based on polypropylene (PP) or similar polyolefins. Experiments were performed to characterize the kinetics of the permeation of low molecular weight model permeants (octene, toluene and isopropanol) from liquid paraffin, through a surrogate potential functional barrier (25 μm-thick oriented PP) into the food simulants olive oil and 3% (w/v) acetic acid. These permeation results were interpreted in terms of three permeation kinetic models regarding the solubility of a particular model permeant in the post-barrier medium (i.e. the food simulant). The results obtained justify the development and evaluation of liquid sub-barrier simulants that would allow flexible yet rigorous testing of new laminated multilayer packaging materials.     

Analysis of benzo[a]pyrene in spiked fatty foods by second derivative synchronous spectrofluorimetry after microwave-assisted treatment of samples

A simple, rapid and inexpensive method has been developed for the determination of benzo[a     

The development of reference materials for paralytic shellfish poisoning toxins in lyophilized mussel. II: Certification study

This paper describes the second part of a project undertaken to develop certified mussel reference materials for paralytic shellfish poisoning toxins. In the first part two interlaboratory studies were undertaken to investigate the performance of the analytical methodology for several PSP toxins, in particular saxitoxin and decarbamoyl-saxitoxin in lyophilized mussels, and to set criteria for the acceptance of results to be applied during the certification exercise. Fifteen laboratories participated in this certification study and were asked to measure saxitoxin and decarbamoyl-saxitoxin in rehydrated lyophilized mussel material and in a saxitoxin-enriched mussel material. The participants were allowed to use a method of their choice but with an extraction procedure to be strictly followed. The study included extra experiments to verify the detection limits for both saxitoxin and decarbamoyl-saxitoxin. Most participants (13 of 15) were able to meet all the criteria set for the certification study. Results for saxitoxin.2HCl yielded a certified mass fraction of <0.07 mg/kg in the rehydrated lyophilized mussels. Results obtained for decarbamoyl-saxitoxin.2HCl yielded a certified mass fraction of 1.59+/-0.20 mg/kg. The results for saxitoxin.2HCl in enriched blank mussel yielded a certified mass fraction of 0.48 +/- 0.06 mg/kg. These certified reference materials for paralytic shellfish poisoning toxins in lyophilized mussel material are the first available for laboratories to test their method for accuracy and performance.     

Announcing a new international peer-reviewed journal:Food Science and Human Wellness now accepting manuscript submission in English

<正>We are pleased to announce the launch of a new international peer-reviewed journal-Food Science and Human Wellness,ISSN 2213-4530,which is an open access journal,produced and hosted by Elsevier B.V.on behalf of Beijing Academy of Food Sciences.Food Science and Human Wellness is an international peer-reviewed English journal that provides a forum for the dissemination of the     

Chinese CTP Market

According to Printing and Printing Equipment Industries Association of China（PEIAC）＇s statistics to the plate manufucturer in China, in 2013, the actual offset plate production has reached 346 million square meters in China. Among them, the CTP production volume was 245 million square meters, up by 11% than that of last year; the total sales of the CTP plate was 239 million square meters, up by 13%.     

Preparatory Work of Print China 2015 is Going Smoothly

正Held at Guangdong Modern International Exhibition Center,Print China 2015 will cover 7exhibition halls,besides the original Hall No.3,4,5,6,7,the newly built F zone of Hall 3 will be used too.The total area will be140,000 square meters.Hall 3:Offset and large printing equipment,package printing equipment,post press