Effect of marinating and vacuum storage at 6 °C on the fate of chemical, microbial and sensory quality indices of echinoid gonads Paracentrotus lividus Lamark, 1816

Chemical, microbial and sensory quality indices of raw and marinated sea urchin Paracentrotus lividus Lamark, 1816, were determined. Marinades were prepared using three different formulations and stored over a 75-day period at 6 °C, under vacuum and air (control) packaging conditions. Significant differences were found between chemical (pH, moisture, ash, NaCI, ammonia and lactic acid), microbial [pseudomonads (Ps), lactic acid bacteria (LAB) and yeast–moulds] and sensory analysis of three different marinated groups ( P  <   0.05) during the storage period. The marinating process did not lead to significant differences in fatty acid, protein, fat and carbohydrate contents relative to raw sample. A notable decrease of the ω-3 per ω-6 ratio after marinating and during storage in both packages was observed. Ammonia concentration changes were found to be useful as index of freshness and decomposition of roe in storage. Shelf life of roe marinades at 6 °C was found to be 75 and 60 days under vacuum and air storage conditions respectively.     

Effect of marinating process on the microbiological quality of Pacific saury (Cololabis saira) during vacuum-packaged storage at 4 °C

This study was carried out to evaluate the microbiological quality of Pacific saury ( Cololabis saira ) treated with marinating solutions containing either 12% NaCl + 2% acetic acid or 12% NaCl + 3% acetic acid, followed by vacuum packaging and storage at 4 °C for 90 days. The fish-to-marinating-solution ratio was 1:1.5. The growth rate of aerobic, halophilic, lactic acid bacteria and Pseudomonas spp. were significantly ( P  < 0.05) reduced in marinated vs. brined (12% NaCl + 0% acetic acid) fillets. Complete inhibition in the growth of both Enterobacteriaceae and Staphylococcus aureus was detected in fillets marinated in 3% and 2% acetic acid by the days 30 and 50 of storage, respectively. Both marinating conditions resulted in shelf life extension of the product to more than 90 vs. 60 days only for the brined fillets. Listeria monocytogenes , Vibrio parahaemolyticus and Salmonella spp. could not be detected in any of the analysed raw or treated samples.     

Effect of modified atmosphere and vacuum packaging on microbial,chemical and sensory quality indicators of fresh,filleted Sardina pilchardus at 3 °C

The self‐life, quality and safety of refrigerated sardine fillets (Sardina pilchardus) were determined at 3 °C in atmospheric air, vacuum and modified atmosphere (50% CO₂/50% N₂) packaging conditions. Microbiological, physico‐chemical and sensory parameters were utilised as quality indicators. The microbial flora of sardine comprised—according to order of occurrence—Shewanella putrefaciens, pseudomonads, Brochothrix thermosphacta, lactic acid bacteria and, finally, Enterobacteriaceae. Bacteria grew most quickly in sardines stored in air, followed by those in vacuum packaging, and the lowest counts were found in modified atmosphere packaging. The concentrations of moisture, ash, protein, fat and polyunsaturated fatty acids were not affected during the storage period compared to the pH values and the concentrations of lactate and ammonia that showed significant differences. Copyright © 2007 Society of Chemical Industry     

不同气调包装对醉虾贮藏品质的影响

研究了40%CO2+60%N2和100%CO2的气调包装以及真空和空气包装4种包装方式对贮藏醉虾菌落总数、理化指标和感官指标的影响。结果表明:相对于真空和空气包装组,100%CO2或40%CO2+60%N2的气体包装,对醉虾的保鲜效果较好,其样品的菌落总数和挥发性盐基氮含量较低,质地和色泽变化较小,感官评分较高。但由于100%CO2包装随着贮藏时间的延长,气体收缩较明显导致产品包装外观不理想,40%CO2+60%N2的混合气体包装更适合于醉虾的贮藏。     

Changes in amino acid and fatty acid composition during shelf‐life of marinated fish

Quality aspects of marinated fish were examined over a 120 day period. Nutritional quality parameters (proximate composition, fatty acid profile and amino acid profile) were determined. Changes in amino acids and fatty acids were examined in the muscle of marinated fish during storage. Aspartic acid, threonine, proline, glycine, tyrosine and lysine concentrations in marinated anchovy and rainbow trout greatly influenced their quality. Polyunsaturated fatty acid concentrations decreased significantly (p < 0.05) in marinated anchovy but not (p > 0.05) in marinated rainbow trout, while total saturated fatty acid concentrations increased significantly (p < 0.05) in both marinated fish during storage. These changes in fatty acid and amino acid concentrations were found to be useful as an index of freshness and decomposition of marinated fish in storage. Copyright © 2005 Society of Chemical Industry     

Effect of film and temperature on the sensory, microbiological and nutritional quality of minimally processed cauliflower

The effect of atmosphere modification, generated using three different packaging films, on the quality of cauliflower minimally processed when stored at 4 or 8 °C for up 20 days was evaluated. The colour, texture, weight loss, sensory attributes, as well as microbial counts and sugars and ascorbic acid content were determined. The atmosphere generated with the perforated polyvinylchloride (PVC) film was hardly modified, whereas the other two films (non-perforated PVC and polypropylene films) originated changes in CO₂ and O₂ levels during storage. The different packaging conditions and storage temperature influenced yellowing. An increase in shear force was observed. Weight losses were below 5%. Mesophiles and Pseudomonas counts were below 7 log CFU g⁻¹, the populations being lower with film B and film C than with film A. Cauliflower maintained an acceptable appearance in all the films studied. Total sugars decreased about 27% after 20 days of storage, whereas ascorbic acid did not change.     

Microbial flora of technological interest in raw ovine milk during 6°C storage

Changes in the microbial flora of ovine milk of medium hygienic quality (initial mean total plate count 3.3  ×  10⁵ cfu/mL) were studied throughout refrigerated storage at 6°C. Total plate counts after 48 h of refrigerated storage (mean count 4.6  ×  10⁵ cfu/mL) were under the current standards in the European Union (EU) for raw ovine milk to be used for cheesemaking after heat treatment. However, after 96 h, mean total plate counts (1.6  ×  10⁷ cfu/mL) were above the standards. Lactococci were found at levels higher than Pseudomonas spp. in milk freshly drawn (54.5% and 3.5% of total plate count, respectively) and after 96 h at 6°C (47.9% and 33.9% of total plate count, respectively). Lactobacilli, enterococci, coliforms and thermodurics were found at values lower than lactococci and Pseudomonas spp., before and after refrigerated storage ( ≤  0.08% of the total plate count). A significant growth ( P  < 0.001) was detected for mesophiles, Pseudomonas spp. and lactococci after 96 h of refrigeration at 6°C; however, thermodurics, coliforms, lactobacilli and enterococci, showed no significant increase ( P  > 0.05). Presumptive Escherichia coli (β-glucuronidase-positive) underwent a decrease throughout storage at 6°C.     

Effect of natural antioxidants and vacuum packaging on the quality of buffalo meat nuggets during refrigerated storage

Buffalo meat nuggets (BMN) were incorporated with 500 ppm sodium ascorbate, 10 ppm -tocopherol acetate and 0.5% sodium tripolyphosphate while processing. There were three experimental groups of BMN, namely control aerobic packaged (CAP), treated aerobic packaged (TAP) and treated vacuum packaged (TVP), refrigerated and stored at 4 °C. It was observed that the treated samples (TAP and TVP) had significantly (p < 0.05) higher pH, W-B shear force, moisture and protein contents, sensory attributes and lower free fatty acids content in comparison to CAP samples. Vacuum packed nuggets (TVP) had significantly (p < 0.05) lower free fatty acid content, higher lactobacilli and total anaerobe counts and markedly higher scores for flavour and overall palatability, even at the 25th day of storage. Product quality reduced as storage time increased. Use of natural antioxidants and vacuum packaging extended the shelf life of BMN from 10 to 30 days under refrigerated storage.     

INFLUENCE OF VACUUM PACKAGING AND ORGANIC ACID TREATMENT ON THE CHILLED SHELF LIFE OF PEARL SPOT (ETROPLUS SURATENSIS, BLOCH 1790)

The present work was carried out to study the effect of vacuum packaging and organic acids drip treatment, namely, sodium acetate and potassium sorbate (2%), on the quality of pearl spot during chilled storage (1–2C). Changes in pH, drip loss, water-holding capacity, total volatile base nitrogen (TVB-N), trimethylamine nitrogen (TMA-N), total plate counts, instrumental texture properties and sensory characteristics were investigated up to 18 days. Sensory evaluation revealed that the potassium sorbate- and sodium acetate-treated vacuum-packaged samples had good sensory characteristics up to 16 days compared with 7 days in control air packs (CAPs) and control vacuum packs (CVPs). The sensory shelf life followed the following order: CAP  <  CVP  <  sodium acetate-treated vacuum pack  <  potassium sorbate-treated vacuum pack. Chemical analysis demonstrated a significant ( P   <  0.05) reduction in TVB-N, TMA-N and total plate counts, and better textural properties in potassium sorbate- and sodium acetate-treated vacuum-packaged fishes than CAPs and CVPs.

PRACTICAL APPLICATIONS

Limited shelf life is one of the major problems the seafood industry faces today. The study compares the effect of vacuum packaging in combination with preservative chemical treatment on the shelf life of pearl spot. The study indicates that the use of chemical preservative treatments such as sodium acetate and potassium sorbate at 2% level, together with vacuum packaging, helps in extending the shelf life of pearl spot to 16 days compared with 7 days in control air packs and control vacuum packs. Hence, vacuum packaging, together with preservative treatment, is very effective in shelf-life extension compared with vacuum packaging alone.     

An investigation of rancidity inhibition during frozen storage of Wels catfish (Silurus glanis) fillets by previous ascorbic and citric acid treatment

The effect of preliminary ascorbic and citric acid (AA and CA, respectively) soaking treatments on the rancidity development in Wels catfish ( Silurus glanis ) fillets during further frozen storage (−18 °C; up to 6 months) was studied. Rancidity development was measured by biochemical quality indices (formation of free fatty acids, peroxides and secondary oxidation products) and compared with sensory analysis (appearance, rancid odour and consistency) and general chemical analyses (pH, expressible moisture and heme iron contents). When compared with control samples, AA- and CA-treated samples showed a lower ( P  < 0.05) formation of primary and secondary lipid oxidation compounds that was corroborated by a longer ( P  < 0.05) shelf-life time as a result of a lower rancid odour formation. According to these results, control samples showed a higher ( P  < 0.05) heme iron breakdown (months 3–6) and a lower ( P  < 0.05) water-holding capacity (higher expressible moisture value) (month 6).     

CHEMICAL CHANGES AND SENSORY EVALUATION OF CHANNEL CATFISH AS AFFECTED BY DIET, PACKAGING METHOD AND FROZEN STORAGE

Fillets from farm-raised channel catfish (Ictalurum punctatus) fed diets containing various amounts of protein were packaged by the following methods: polyvinylidene chloride (PVDC) film overwrapping, vacuum packaging with Eva bag and vacuum skin packaging. Packaged fillets were stored at —28C for 90 days. Chemical and sensory changes were determined. Neither the packaging method nor protein content of diet had a significant (p < 0.05) effect on TBA number of frozen stored catfish. No significant differences on free fatty acid content of catfish packaged in different methods were also found. However, sensory evaluation revealed that greasiness of cooked catfish decreased as toughness of fillet texture increased throughout entire frozen storage.     

包装方式对四角蛤蜊卤制风味产品贮藏品质的影响

目的：研究不同包装方式对其贮藏期品质的影响。方法 对四角蛤蜊卤制风味产品进行普通包装、真空包装、30% CO2+70% N2、50% CO2+50% N2、30% N2+70% CO2气调包装,并在4 ℃贮藏条件下,测定菌落总数、质构特性、色差、TVB-N值、风味特性等理化指标,选择最佳的包装方式。结果 普通包装样品在第3 d、真空包装组样品在第6 d时菌落总数超标,而气调包装组的货架期可达12 d左右。气调包装组的硬度、弹性、咀嚼性的下降程度都低于普通包装和真空包装组,气调包装的护色效果也优于其他两组。气调包装对TVB-N值、TBA值、pH值变化的延缓作用明显优于真空包装和普通包装。结论 不同包装方式对四角蛤蜊卤制风味产品贮藏品质的保持能力依次为气调包装、真空包装、普通包装,其中50% CO2+50% N2气调包装保鲜效果最佳,能显著延长产品货架期。     

The sensory quality of chicken and potato products prepared using cook–chill and sous vide methods

Summary This study examined the effects of sous vide (i.e. vacuum packaging) upon the sensory characteristics of chicken breast and of sliced potatoes in cream both immediately after cooking and following subsequent chilling, chilled storage and reheating. Cooking was to a range of end point temperatures (70 °C for 2 minutes, 80 °C for 10 minutes, 80 °C for 30 minutes) and chilled storage was for 2,5 or 7 days at 5 °C. The products were evaluated by separate sensory panels, each comprising either 25 or 10 trained assessors using attribute scaling methods. With both 80 °C heat treatments, sous vide significantly increased the flavour intensity of both products ( P < 0.05) and the juiciness and moistness of the chicken and potato respectively ( P < 0.05) compared to nonvacuum packed freshly cooked products. In general, higher hedonic scores were associated with higher flavour and juiciness scores for chicken and with higher flavour and moisture scores for potato. The hedonic appeal results indicate that, contrary to what has been widely assumed, these changes may not always be desirable in terms of product acceptability. The data also indicate that, in some products, the heat treatments necessary to ensure microbiological safety cause an appreciable loss of sensory quality.     

Effect of Brining, Modified Atmosphere Packaging, and Superchilling on the Shelf Life of Cod (Gadus morhua) Loins

ABSTRACT:  The aim of these experiments was to evaluate the effect of brining, modified atmosphere packaging (MAP), and superchilling on the quality changes of cod loins as measured by microbial, sensory, and chemical analysis. Unbrined and brined (2.5 ± 1.0% NaCl) cod loins were kept in styrofoam boxes (air) and under modified atmosphere (MA, CO₂/O₂/N₂: 50/5/45) at 0, −2, and −3.6 °C. Samples were examined over a 4-wk period. Total viable psychrotrophic counts and counts of H₂S-producing bacteria reached higher numbers in the air-packed brined fish at −2 and −3.6 °C than in comparable unbrined groups, being significantly different ( P  < 0.05) at the lower temperature. However, lower counts of these bacteria were obtained in the brined MAP fish than in comparable unbrined fish. Counts of  Photobacterium phosphoreum  increased most rapidly in air- and MA-packed loins kept at 0 °C. Lower counts were found at superchilled temperatures. According to sensory analysis the shelf life of unbrined air-packed loins was about 11 d at 0 °C and 14 to 15 d at −2 °C. The shelf life of MA-packed unbrined loins was about 14 to 15 d at 0 °C but 21 d at −2 °C. Thus, synergism of combined superchilling (−2 °C) and MA led to a considerable shelf life increase for unbrined loins despite the fact that processing and packaging took place 4 to 5 d post-catch. The shelf life of air-packed brined loins at −2 °C was 12 to 15 d but only 13 d under MA. The same synergistic effect did therefore not apply to brined loins as with unbrined ones.     

THE QUALITY CHARACTERISTICS OF M. LONGISSIMUS FROM HANWOO (KOREAN CATTLE) WITH DIFFERENT FLOOR SPACE

The quality characteristics of Musculus longissimus from Hanwoo bulls with different floor space were studied. Fifteen heads of bulls were randomly divided into three groups (9, 18 and 27 m²). At 48 h postslaughter, samples were collected and stored at 4  ±  0.5C for 9 days. The n6 / n3 polyunsaturated fatty acid of the meat from cattle in 27 m² was significantly lower ( P <  0.05) than that in other groups. The meat of cattle in 27 m² contained more C20:5 n-3 eicosapentaenoate (EPA) ( P <  0.05) than the meat from cattle in 9 m². The pH value and drip loss of the meat from cattle in 27 m² were significantly higher ( P <  0.05) than those in other groups. The 2-thiobarbituric acid reactive substances and peroxide values of the meat from cattle in 27 m² were significantly lower ( P <  0.05) than those in other groups at 6 and 9 days of storage. The increasing floor space from 9 to 27 m² improved the fatty acid composition, water-holding capacity and lipid stability of the meat.

PRACTICAL APPLICATIONS

Meat quality is one of the important values related to human well-being. Floor space is one of the important factors in animal welfare that affect beef quality. Increasing the floor space from 9 to 27 m² for bulls during the finishing period had improved the meat quality, especially in respect to fatty acid composition, water-holding capacity and lipid oxidation resistance.     

冷冻贮藏过程中包装处理对脆肉鲩鱼片品质的影响

对脆肉鲩鱼片在4种包装处理(镀冰衣+真空包装、不镀冰衣+真空包装、镀冰衣+非真空包装、不镀冰衣+非真空包装)下冻藏(-18℃)8 w过程中,理化、质构和感官品质的变化进行了研究。不同包装处理对干耗和TBA值的变化有显著影响。不同包装条件下粘着性无显著差异,而硬度、咀嚼性和回复性因受包装处理方式不同而存在显著差异。随着冻藏时间的延长。包装的条件对脆肉鲩鱼片各感官指标影响较大。与镀冰衣相比较,真空包装能更有效地防止脆肉鲩鱼片冻藏过程中理化、质构及感官品质的劣化,若在鱼片冻藏过程中采用镀冰衣与真空包装相结合的方法则能更有效地防止品质的劣化。     

EXTENDING THE CHILLED SHELF LIFE OF VACUUM-PACKAGED GROUND BEEF USING ASCORBIC ACID, NITRITE OR SALT

The objective of this study was to extend the shelf life of ground beef in chilled conditions. Samples were treated with 2% salt, 2% salt  +  500-ppm ascorbic acid, or 2% salt  +  100-ppm sodium nitrite. The control sample did not contain any additives. Protein, nitrite, moisture contents, pH, thiobarbituric acid (TBA) value, color attributes and microbial counts of samples were determined. Samples were vacuum packaged and stored in refrigeration, and chemical and microbial analyses were performed throughout storage. The pH of samples decreased during storage, and the sample having 2% salt and 500-ppm ascorbic acid had the greatest pH decline. TBA values generally increased ( P <  0.05) with storage time. Samples having 2% salt  +  500-ppm ascorbic acid had the lowest TBA value. On a given storage day, samples having 2% salt  +  100-ppm sodium nitrite had lower microbial microbial count than other samples, indicating the antibacterial effect of nitrite. A significant storage time  ×  treatment interaction ( P <  0.05) existed for almost all quality parameters studied.

PRACTICAL APPLICATIONS

Ground beef has commercial importance for the meat industry in Turkey. Meat processors have difficulties in marketing ground beef because of its relatively low shelf life. By adding ascorbic acid or salt, it is possible to extend shelf life and preserve its color, and to limit lipid oxidation, which will collectively help to increase market share of ground beef.     

Study of the shelf life of a dry fermented sausage "salchichon" made from raw material enriched in monounsaturated and polyunsaturated fatty acids and stored under modified atmospheres

The microbiological, physico-chemical and sensory properties of salchichon with high unsaturated fat content, packed under vacuum and 20/80% CO(2)/N(2) modified atmosphere, were evaluated to determine its quality changes during storage under refrigeration. These sausages were manufactured with pork meat and pork backfat obtained from pigs fed with three different diets (control diet-CO, high oleic diet-HO and high linoleic diet-HL). In general, few significant differences were found in counts of different groups of microorganism between the three types of sausages and no difference between the packaging methods. A reduction in pH values was observed during storage and no great differences were determined by storage period on water activity (a(w)). Both parameters (pH and a(w)) presented similar results to those found in different Spanish sausages and other European dry fermented products. The sensory results denoted that sensory quality gradually decreased during storage under both packaging conditions (vacuum and 20/80% CO(2)/N(2)), so it is not advisable to store longer than 150days. On the other hand, fermented sausages with high content of unsaturated fatty acids had similar sensory properties to those of conventional sausages, and even a comparable sensory stability. In conclusion, the results showed healthier salchichons (HO and HL) similar to the traditional (CO) one could be manufactured and stored under refrigeration after slicing for a reasonable period, but the advantage of the gas mixture packaging (20/80% CO(2)/N(2)) versus vacuum packaging was not clear.     

Shelf life of Turkish whey cheese (Lor) under modified atmosphere packaging

In this study, the shelf life of Lor cheese stored under different atmosphere compositions was assessed and compared. Lor cheeses were held in four different atmospheres containing: vacuum packaging (VP), 40% CO₂/60% N₂, 60% CO₂/40% N₂ and 70% CO₂/30% N₂ (modified atmosphere packaging). Control cheeses were stored in air. All cheese samples were kept in the refrigerator at 4°C for 45 days and investigated for physicochemical, microbiological and sensory properties. The acidity index value was significantly higher ( P  < 0.05) in the control and vacuum packaged samples than in those stored for the same period under CO₂. Microbiological results showed that modified atmosphere packaging delayed microbial growth compared with air and VP samples. Of the three modified atmospheres, gas mixtures 60% and 70% CO₂ were the most effective for inhibition of growth of micro-organisms. Sensory evaluation (odour and taste) results showed that Lor cheese packaged under modified atmosphere packaging (60% CO₂/40% N₂ and 70% CO₂/30% N₂ ) retained good characteristics for 45 days of storage, while vacuum and control samples were sensorily unacceptable after 10 days of storage.     

微冻及真空包装对原汁整壳贻贝贮存期品质的影响

为研究微冻及真空包装对原汁整壳贻贝贮存期品质的影响,将原汁整壳贻贝进行真空包装及普通包装,于4和-3℃下进行贮存,对样品的感官评分、汁液流失率、pH、电导率、挥发性盐基氮(total volatile basic nitrogen,TVB-N)、2-硫代巴比妥酸(2-thiobarbituric acid,TBA)、菌落总数等指标的变化规律进行分析。结果表明:原汁整壳贻贝在贮藏过程中,随着时间的延长,感官评分下降;pH先降低后升高;汁液流失率、TVB-N、TBA、电导率和菌落总数均随贮存时间的延长而增长。-3℃微冻的保鲜效果明显优于4℃冷藏(P<0.05),真空包装的保鲜效果明显优于普通包装(P<0.05),-3℃微冻结合真空包装保鲜效果最佳,能显著延缓原汁整壳贻贝感官性状的下降(P<0.05),抑制汁液流失率、电导率、TVB-N值、TBA值的上升(P<0.05),延缓pH最低点出现的时间(P<0.05),抑制菌落总数的增长(P<0.05)。综合比较分析,4℃普通包装、4℃真空包装、-3℃普通包装、-3℃真空包装的货架期分别为6、15、15和60 d。因此,微冻结合真空包装能较好维持原汁整壳贻贝的感官性状及品质稳定性,有效延长货架期。