Physicochemical and sensory properties of restructured beef steaks containing beef heart surimi

Summary Restructured beef steaks containing beef heart surimi were prepared. Changes in flavour, texture, thiobarbituric acid-reactive substances (TBARS), and compression force of the restructured steaks were evaluated after frozen storage (−29 °C) for 1, 30, and 90 days. Incorporation of propyl gallate-washed beef heart surimi improved the steak flavour score by 7%, texture score by 30%, and cooking yield by 4%, compared to non-surimi control steaks. Addition of water-washed surimi (without propyl gallate) also improved physical properties of restructured steaks, but it induced off-flavour and rancidity development.     

Biogenic amines in restructured beef steaks as affected by added walnuts and cold storage

This article evaluates changes in biogenic amines and how these relate to microbiological growth in chilled, fresh restructured beef steaks containing transglutaminase as a cold binding agent and different amounts of walnut. Added walnut and chilling favored higher total and lactic acid bacteria counts during storage, whereas Enterobacteriaceae were not affected. The highest initial biogenic amine concentrations were identified as spermidine, spermine, and tyramine. Both added walnut and cold storage generally favored the formation of amines (tyramine, histamine, putrescine, and cadaverine), which was more obviously apparent by the end of the storage period. Agmatine, on the other hand, was not generally affected by the walnut.     

Physicochemical and sensory characteristics of restructured beef steak with added walnuts

The effects of different proportions (0, 5, 10, 15%) of added walnuts on the physicochemical and sensory characteristics of restructured beef steak were evaluated. The addition of 10 and 15% walnut reduced (P<0.05) cooking loss. Increasing proportions of walnut in the beef steak increased (P<0.05) water binding and reduced (P<0.05) fat binding properties. The addition of walnut did not affect (P>0.05) textural properties in uncooked restructured beef steak, but in cooked products Kramer shear force and bind strength were reduced (P<0.05) when 10% or more of walnut was added. Product morphology characteristics suggest that walnut interferes with the formation of protein network structures. Restructured steaks made with added walnut presented acceptable sensory properties. Incorporation of nuts in meat products can be use to confer potential heart-healthy benefits (Spanish Patent Application 200300367).     

Nutritional profile of restructured beef steak with added walnuts

Amino acid, fatty acid profile, cholesterol, vitamin E and mineral contents were assessed in restructured beef steak with 20% added walnut (20W). Compared with control restructured beef steak (0% added walnut), the product with added walnut presented a lower (P < 0.05) lysine/arginine ratio, larger (P < 0.05) quantities (mg/100 g product) of monounsaturated (MUFA) and n3 polyunsaturated (PUFA) fatty acids (mainly -linolenic acid), a lower (P < 0.05) n6/n3 PUFA ratio and a higher (P < 0.05) polyunsaturated/saturated fatty acid ratio. The replacement of raw meat material by walnut reduced (P < 0.05) the cholesterol content and increased (more than 400 times) the amount of γ-tocopherol. Iron, calcium, magnesium and manganese contents of 20W sample were greater (P < 0.05) than in the control. Some changes induced by added walnut in the nutritional quality of the restructured product may present health benefits.     

Frozen storage stability of antioxidant-treated raw restructured beef steaks made from mature cows

Previous research has shown that beef quality decreased with the age of cattle. In this study, beef trimmings from nine mature cows (n = 9), equally representing three animal age groups (2–4, 6–8, and 10–12 yr), were restructured into steaks formulated with propyl gallate, alone or in combination with a beefy flavoring agent, to enhance palatability and stability during 6 months of frozen storage at −29 °C. Lipid oxidation, rancidity, and loss of beefy flavor in restructured steaks during extended storage were reduced by propyl gallate. The beefy flavoring agent inclusion masked mature, forage-fed beef off-flavors, intensified beefy flavor, and improved steak tenderness, juiciness and cooking yield. Thus, the combination of propyl gallate and beefy flavoring offers an effective means to enhance the palatability and storage stability of restructured beef prepared from mature cows.     

Frozen storage stability of vacuum-packaged precooked restructured steaks manufactured from mature cow beef

Beef trimmings from nine cows, equally representing three animal age groups (2-4, 6-8, and 10-12 year), were restructured into steaks formulated with propyl gallate, alone or in combination with a beefy flavoring agent, to enhance palatability and storage stability. Steaks were fully cooked prior to being stored under vacuum for 6 months at −29 °C. Animal age did not affect steak quality and lipid stability, whereas sensory palatability scores and reheating yield decreased significantly beyond 3 months. Propyl gallate did not impart any additional protection against lipid oxidation beyond that provided by vacuum packaging. On the other hand, inclusion of beefy flavoring agent masked all mature, forage-fed beef off-flavors, intensified beefy flavor and improved steak tenderness, juiciness and reheating yield. The incorporation of a beefy flavoring agent was shown to be an effective means to enhance the palatability of vacuum packaged precooked steaks manufactured from mature cows.     

The effect of calcium carbonate and sodium alginate on the color and bind strength of restructured beef steaks

This study investigated the effect of calcium carbonate concentration (0·00-0·26%) and sodium alginate concentration (0·0-1·4%) on the amount of discoloration and the raw and cooked bind-strength of restructured beef steaks. Alginate slightly increased and calcium carbonate markedly decreased the amount of discoloration in the restructured steaks. The protective effect of the calcium carbonate did not increase with increasing concentration and did not appear to be due to increased pH. The amount of discoloration in restructured steaks prepared with alginate was similar to that in conventionally prepared restructured steaks. The optimum concentration of calcium carbonate and alginate required for minimum discoloration and maximum raw and cooked bind-strength was 0·13% and 0·7%, respectively.     

Composition and physicochemical characteristics of restructured beef steaks containing walnuts as affected by cooking method

The study was conducted to determine the influence of various cooking methods - conventional oven, microwave oven, electric grill and pan-frying on the composition and physicochemical characteristics of restructured beef steaks formulated as low fat (L, 2.0% fat), medium fat (M, 13.0% fat) and 20% added walnut (W, 12.6% fat). Composition, retention coefficients for different compounds, cooking loss (CL) and texture were affected by both formulation and cooking. There was considerable retention of moisture, fat and minerals (as ash) in W samples after cooking. CL was highest (P<0.05) in M sample and lowest (P<0.05) in W samples. Microwaved restructured steaks had higher (P<0.05) kramer shear force (KSF) and bind strength (BS), while pan-fried and conventionally oven-cooked steaks had lower (P<0.05) KSF. The thiobarbituric acid-reactive substances (TBARS) content was low in all restructured steaks but was affected by both cooking and formulation. Although cooking can change the proximal composition of steaks, lipid retention (>90%) was greatest in the case of the W sample, thus assuring that the ultimate objective of these restructured meats, namely to provide a source of walnut and walnut fat, would be successful.     

Effect of long frozen storage on the formation of triglyceride alteration compounds of pan-fried functional restructured beef steaks

The influences of pan-frying in extra virgin olive oil (EVO) and frozen storage of functional restructured beef steaks on the contents of polar material (PM) and thermal oxidation compound of the fat extracted from them were determined. Two types of functional restructured meats: 20% walnut-added (W, 13% fat) and low fat (L, 2% fat) were studied in comparison to conventional medium fat (M, 13% fat) restructured beef steaks. Differences in the PM or the thermal oxidation and hydrolytic compounds were mainly due to meat formulation with fried L samples showing the highest PM and polymer concentrations and fried W steaks the lowest. After one year of frozen storage the PM content increased in all samples but markedly in L and W steaks. Polymers decreased in L and increased in W and M steaks. Thermal oxidation compounds increased in W and M steaks while hydrolysis increased markedly in L samples. Long-term storage of all fried steaks is not recommended, whether functional of not since lipid degradation occurs. Although these alterations after one year-frozen storage are still far from their discarding limit, the consumption of just cooked functional steaks is highly recommended.     

Effects of lactate-enhancement on surface reflectance and absorbance properties of beef longissimus steaks

A completely randomized block design was used to assess the effects of lactate-enhancement on surface reflectance and absorbance properties of beef longissimus steaks. Loins were divided into sections, assigned to one of four treatments (non-enhanced day 0, non-enhanced day 5, water-enhanced, and 3% lactate), vacuum packaged, stored for 5 days at 4 °C, and then cut into steaks that were used to prepare 100% of deoxy-, oxy-, met-, and carboxymyoglobin according to AMSA (1991). Surface color was analyzed using a HunterLab Miniscan Plus Spectrocolorimeter. Lactate-enhanced steaks had the least overall surface reflectance and the darkest surface color (lower L*; P < 0.05). For 100% of each myoglobin form, K/S values and ratios (isobestic wavelengths ÷ 525 nm) at 474, 525, 572, and 610 also were influenced by lactate-enhancement. Hence, when estimating surface myoglobin forms using K/S ratios, separate 100% myoglobin reference standards should be prepared from both non-enhanced and enhanced steaks.     

Dietary vitamin E supplementation effects on the color and sensory characteristics of enhanced beef steaks

The objective of this study was to evaluate the effects of dietary vitamin E supplementation on characteristics of enhanced beef cuts during retail display. Twelve steers were fed either a control (E-) diet or a diet supplemented with dl-alpha tocopheryl acetate (E+) for 117 days prior to slaughter. Paired strip loins, clods, and inside rounds served as the control (C) or were pumped (P) to 110% of raw weight to contain 0.4% sodium chloride and 0.4% phosphate on a finished weight basis. Steaks were cut (2.5 cm) for sensory evaluation and retail display. No flavor or texture differences existed in strip steaks due to vitamin E. Enhanced steaks were more tender, juicy and salty than controls, however they discolored more rapidly than did controls. Steaks from supplemented cattle were slightly, but significantly, less discolored, indicating that vitamin E may provide some improvements in color stability of enhanced meat products. Vitamin E supplementation may improve short term color stability (up to 2 days in display) of retail beef enhanced by injection of a salt-phosphate solution.     

Properties of restructured beef steaks from forage- and grain-fed cattle as affected by antioxidant and flavoring agents

Beef trimmings from cattle finished on forage or grain were restructured into steaks to enhance palatability. Steaks were treated with propyl gallate with or without a beefy flavoring agent, stored at -29?°C, and analyzed after 0, 1, 3, and 6 months. The strong grassy flavor of forage-finished beef steaks, detected by a sensory panel, was masked by the beefy flavoring agent, rendering the beef more acceptable by consumers. Propyl gallate retarded lipid oxidation and rancidity development in steaks during extended frozen storage. Microbial populations decreased while color scores, cooking yield, and binding strength of steaks exhibited only minor changes during storage. Thus, the combination of antioxidant and flavoring agents with the muscle restructuring technology provides an effective means to enhance the palatability and storage stability of beef from forage-fed cattle.     

Effect of acid decontamination of beef subprimal cuts on the microbiological and sensory characteristics of steaks

Beef strip loins were decontaminated by spraying with solutions of various food grade acids (1.0% lactic acid, 1.0% acetic acid and an acid mixture containing 1.0% lactic acid, 2.0% acetic acid, 0.25% citric acid and 0.1% ascorbic acid) followed by vacuum packaging and storing at 4 ± 1°C. Initially and at days 3 and 6 of display in polyvinyl chloride (PVC) film, aerobic plate counts (APCs) of steaks fabricated from the acid treated loins that were stored for 0, 14, 28, 42, 56, 70 and 84 days were not significantly different (P > 0.05) from the APCs of steaks fabricated from control loins.     

Microbiological and sensory characteristics of beef loin steaks: Role of subcutaneous fat

Beef loin steaks with the subcutaneous fat attached, without subcutaneous fat and the subcutaneous fat that was removed from steaks were packaged and stored at 4°C ± 1°C in polyvinyl chloride (PVC) film for 0–6 days and in high-oxygen barrier (HOB) film for 0–28 days. Aerobic plate counts (APCs) of subcutaneous fat of intact steaks and of subcutaneous fat that was packaged and stored separately in PVC and HOB films were greater (P < 0·05) than those of comparable lean samples. The APCs of lean of steaks without subcutaneous fat that were packaged and stored in HOB film were lower (P < 0·05) than those of the lean of intact steaks. APCs of the lean of these two types of steaks packaged and stored in PVC film did not differ (P > 0·05). Mean surface discoloration and mean overall appearance scores of intact steaks packaged and stored in HOB film were greater than those of steaks packaged and stored without subcutaneous fat; differences were significant (P < 0·05) after 21 and 14 days, respectively. This difference in surface discoloration was attributed to metmyoglobin formation due to possibly higher levels of oxygen remaining in the packages of steaks without subcutaneous fat than in packages containing steaks with the fat attached.     

Microbiological and sensory characteristics of beef loin steaks: Role of intramuscular fat

Beef loin steaks of different grades (Prime, Choice and Good) were packaged and stored in polyvinyl chloride (PVC) film for 0-6 days and in high-oxygen barrier (HOB) film for 0-28 days. Grade had no significant effect (P > 0·05) on the aerobic plate count and did not result in major differences in the distribution of types in the microflora of steaks. Of the sensory characteristics examined, mean surface discoloration and mean overall appearance scores of Prime and Choice steaks packaged and stored in HOB film often were higher (P < 0·05) than those of Good steaks. Differences in metmyoglobin formation among steaks from the three grades were attributed to differences in the inherent characteristics of the muscles; muscle fibers from Prime and Choice samples were probably more red (as evidenced by greater marbling ability), while muscle fibers of Good samples were probably more white (as evidenced by lesser marbling ability). Red muscles have greater cytochrome activity, which will help reduce metmyoglobin to myoglobin in the absence of oxygen.     

Establishing tenderness thresholds of Venezuelan beef steaks using consumer and trained sensory panels

The tenderness thresholds for ribeye steaks at 2 d postmortem were found by using sensory trained (n = 734 steaks) and consumers panel data (55 ribeye steaks and 296 consumers). Also, consumers’ perceptions of sensory attributes of beef steaks were assessed to establish their relationships and to determine acceptability levels. The tenderness threshold obtained by regression analysis from trained panelists was 37.98 N (3.87 kg). Customer satisfaction of 81% was obtained when beef steaks had Warner-Bratzler shear force values (WBSF) less than 40.13 N (4.09 kg), suggesting that 40.13 N could be considered as a tenderness threshold to segregate tough from tender steaks, for Venezuelan beef consumers. According to correlation and regression analyses, consumers focused on flavor to assign their overall liking score. Results suggest that consumers are able to detect changes in tenderness of steaks with different WBSF and that flavor drives customer satisfaction when the beef tenderness is acceptable.     

Sodium hydroxide and sodium tripolyphosphate effects on bind strength and sensory characteristics of restructured beef rolls

Restructured beef rolls formulated with 1% NaCl (controls) or with 1% NaCl plus 0.07% NaOH or 0.375% sodium tripolyphosphate (STPP) had different (p < 0.05) relative bind strength and cooked yield, as follows: STPP > NaOH > controls. Percent cooked yield was inversely affected (p < 0.05) by added water level (5 > 10 > 20%). Bind values were lower (p < 0.05) in rolls with 20% added water. NaOH and STPP rolls had higher pH (p < 0.05) than controls (6.28, 6.22, and 6.07, respectively). Panel cohesiveness, juiciness, and acceptability scores were also generally higher (p < 0.05) for NaOH and STPP rolls, compared to controls. There was a high correlation (0.93) between panel cohesiveness scores and instrumental bind values. At 20% added water, STPP rolls were preferred, but at 10% added water, STPP and NaOH rolls were similar in overall acceptability. Thus, if added water level is not too high, NaOH alone or perhaps with other binding agents may be an alternative to phosphates in cooked beef rolls.