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1.
Regina Bilan Prof. Dr. Igor Nabiev Dr. Alyona Sukhanova 《Chembiochem : a European journal of chemical biology》2016,17(22):2103-2114
Quantum dots (QDs) are highly fluorescent nanocrystals with advanced photophysical and spectral properties: high brightness and stability against photobleaching accompanied by broad excitation and narrow emission spectra. Water‐soluble QDs functionalized with biomolecules, such as proteins, peptides, antibodies, and drugs, are used for biomedical applications. The advantages of QD‐based approaches to immuno‐histochemical analysis, single‐molecule tracking, and in vivo imaging (over traditional methods with organic dyes and fluorescent proteins) are explained. The unique spectral properties of QDs offer opportunities for designing systems for multiplexed analysis by multicolor imaging for the simultaneous detection of multiple targets. Conjugation of drug molecules with QDs or their incorporation into QD‐based drug‐delivery particles makes it possible to monitor real‐time drug tracking and carry out image‐guided therapy. Because of the tunability of their photophysical properties, QDs emitting in the near‐infrared have become an attractive tool for deep‐tissue mono‐ and multiphoton in vivo imaging. We review recent achievements in QD applications for bioimaging, targeting, and drug delivery, as well as challenges related to their toxicity and non‐biodegradability. Key and perspectives for further development of advanced QD‐based nanotools are addressed. 相似文献
2.
Changhyun Roh Ho‐Young Lee Sang‐Eun Kim Sung‐Kee Jo 《Journal of chemical technology and biotechnology (Oxford, Oxfordshire : 1986)》2010,85(8):1130-1134
BACKGROUND: Over 170 million people, more than 3% of the world's population, suffer from the hepatitis C virus (HCV) infection and the rate of death from liver‐related mortality to HCV has increased. In respect of this, the development of assays for biological imaging should be urgently considered as an essential factor in diagnosis. RESULTS: A novel HCV‐detecting technique using a nanoparticle‐supported aptamer probe was demonstrated. With the aid of nanoparticle quantum dots (QDs) with carboxyl group as an imaging probe, and 5′‐end‐amine‐modified RNA oligonucleotide as a capturing probe, target HCV NS3 was visually detected on chip. The QDs‐based RNA aptamer for HCV NS3 showed high selectivity and specificity against other protein such as BSA. The detection limit of HCV NS3 protein was 5 ng mL?1 level. CONCLUSION: With a novel strategy for protein–aptamer interaction, the feasibility of applying QDs‐based fluorescent detection technique to HCV viral protein assay for the development of a protein biochip was demonstrated. This scheme of QDs‐mediated imaging with a target‐oriented specific RNA aptamer for the detection of infectious HCV diseases provides an efficient strategy and a promising new platform for monitoring applications. Copyright © 2010 Society of Chemical Industry 相似文献
3.
Transparent poly(styrene‐butadiene‐styrene) (SBS)‐quantum dots (QDs) composites (SBS/CdTe QDs) that simultaneously possess strong photoluminescence (PL) and enhanced mechanical properties are presented for the first time based on the facile blending of SiO2‐hybridized CdTe QDs with SBS. UV–vis spectrum and fluorescence measurement show that SBS/CdTe QDs composites exhibit good optical properties. The results of transmission electron microscopy show good dispersion of CdTe QDs in the SBS matrix. The results of dynamic mechanical thermal analysis indicate that the micro‐phase separated structure of the SBS is exist in the composites, and the presence of CdTe QDs can lead to an decrease of glass transition temperatures of polybutadiene (PB) and polystyrene(PS) domains. In addition, mechanical tests reveal that the addition of CdTe QDs is a useful approach to improve the mechanical properties of SBS. Meanwhile, the fluorescent photographs taken under ultraviolet light prove that SBS/CdTe QDs composites possess strong PL. © 2011 Wiley Periodicals, Inc. J Appl Polym Sci, 2011. 相似文献
4.
Jie Chen Liangliang Zhu Heyi Xie Jing Zhang Yongsheng Mao Zhenhong Huang Bo Shi Su Chen 《Polymer International》2014,63(11):1953-1958
We report a facile strategy for fabricating fluorescent quantum dot (QD)‐loaded microbeads by means of microfluidic technology. First, a functional fluorine‐containing microemulsion was synthesized with poly[(2‐(N‐ethylperfluorobutanesulfonamido)ethyl acrylate)‐co‐(methyl methacrylate)‐co‐(butyl acrylate)] (poly(FBMA‐co‐MMA‐co‐BA)) as the core and glycidyl methacrylate (GMA) as the shell via differential microemulsion polymerization. Then, CdTe QDs capped with N‐acetyl‐l ‐cysteine (NAC) were assembled into the poly(FBMA‐co‐MMA‐co‐BA‐co‐GMA) microemulsion particles through the reaction of the epoxy group on the shell of the microemulsion and the carboxyl group of the NAC ligand capped on the QDs. Finally, fluorescent microbeads were fabricated using the CdTe QD‐loaded fluorine‐containing microemulsion as the discontinuous phase and methylsilicone oil as the continuous phase by means of a simple microfluidic device. By changing flow rate of methylsilicone oil and hybrid microemulsion system, fluorescent microbeads with adjustable sizes ranging from 290 to 420 µm were achieved. The morphology and fluorescent properties of the microbeads were thoroughly investigated using optical microscopy and fluorescence microscopy. Results showed that the fluorescent microbeads exhibited uniform size distribution and excellent fluorescence performance. © 2014 Society of Chemical Industry 相似文献
5.
Fabrication of fluorescent poly(l‐lactide‐co‐caprolactone) fibers with quantum‐dot incorporation from emulsion electrospinning for chloramphenicol detection
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The on‐site rapid detection of antibiotic residues deposited in food or beverage still remains a challenge in daily life. In this study, cadmium tellurium (CdTe) quantum dots (QDs) were incorporated into poly(l ‐lactide‐co‐caprolactone) (PLLACL) fibers with emulsion electrospinning. Water‐soluble CdTe QDs were used as fluorescence agents, and PLLACL was used as filament materials, respectively. A variety of experiments were performed to characterize the structure and properties of the fibrous composite. Ultraviolet–visible and photoluminescence spectra of the fibers showed similar characteristic absorption and emission properties to those of the CdTe QDs. The fibrous QD–PLLACL composite showed stable fluorescence over 30 days at room temperature and could be used to detect chloramphenicol through fluorescence quenching caused by resonance energy transfer. This approach provides a facile shortcut for fabricating fluorescent fibers that simultaneously inherit the mechanical behavior of PLLACL fibers and the fluorescence properties of CdTe QDs for the detection of antibiotics. © 2016 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2017 , 134, 44584. 相似文献
6.
Alternative procedure for the incorporation of quantum dots into poly(N‐isopropyl acrylamide‐co‐acrylic acid) microgels based on multiple interactions
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Monodisperse fluorescent poly(N‐isopropyl acrylamide‐co‐acrylic acid) microgels doped with quantum dots (QDs) were fabricated as follows. First, cysteamine‐capped cadmium telluride (CA–CdTe) QDs were introduced into the microgels at pH 7 by electrostatic interactions. Afterward, the CA–CdTe QDs were further immobilized in the microgels by the collapse of the polymer network when the pH of solution was adjusted to 4. In this system, there existed multiple interactions between the CA–CdTe QDs and the microgels, including hydrogen bonds, electrostatic interactions, and coordination bonds. The photoluminescence intensity and maximum emission wavelength of the resulting microgels could be easily adjusted by changes in the content of the CA–CdTe QDs in the hybrid microgels (HMs) and with differently sized QDs, respectively. We found that the lower the addition of CA–CdTe QDs was, the bigger the blueshift of the photoluminescence spectra of the HMs was and the weaker the photoluminescence intensity was. Finally, temperature‐responsive emission of the HMs was examined. © 2015 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2016 , 133, 43227. 相似文献
7.
A Coumarin‐Labeled Vinyl Sulfone as Tripeptidomimetic Activity‐Based Probe for Cysteine Cathepsins
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Matthias D. Mertens Janina Schmitz Dr. Martin Horn Norbert Furtmann Prof. Dr. Jürgen Bajorath Dr. Michael Mareš Prof. Dr. Michael Gütschow 《Chembiochem : a European journal of chemical biology》2014,15(7):955-959
A coumarin‐tetrahydroquinoline hydride 8 was synthesized as a chemical tool for fluorescent labeling. The rigidified tricyclic coumarin structure was chosen for its suitable fluorescence properties. The connection of 8 with a vinyl sulfone building block was accomplished by convergent synthesis thereby leading to the coumarin‐based, tripeptidomimetic activity‐based probe 10 , containing a Gly‐Phe‐Gly motif. Probe 10 was evaluated as inactivator of the therapeutically relevant human cysteine cathepsins S, L, K, and B: it showed particularly strong inactivation of cathepsin S. The detection of recombinant and native cathepsin S was demonstrated by applying 10 to in‐gel fluorescence imaging. 相似文献
8.
Dr. Chao Wang Dr. Xinbo Song Prof. Yi Xiao 《Chembiochem : a European journal of chemical biology》2017,18(17):1762-1769
Genetically encoded technologies provide methods for the specific labeling and imaging of proteins, which is essential to understand the subcellular localization of these proteins and their function. Herein, we employed naphthalimide, an efficient two‐photon fluorophore, to develop O6‐benzylguanine (BG) derivatives for specific labeling of subcellular proteins and fluorescent imaging through the SNAP‐tag. Three naphthalimide–BG derivatives, TNI‐BG, QNI‐BG, and ONI‐BG, were conveniently synthesized through modular “click chemistry” in high yields. All of them showed high labeling efficiency with SNAP‐tag in solution (≈1–2×103 s?1 m ?1) and in bacteria. Among them, ONI‐BG showed high specificity to diffused, histone H2B and mitochondria COX8A targeted SNAP‐tag in mammalian cells. The protein‐labeled naphthalimides exhibited high two‐photon absorption cross‐sections, which indicated their potential application in protein‐specific two‐photon fluorescent imaging, such as two‐photon fluorescent lifetime imaging and two‐photon multicolor imaging. Therefore, ONI‐BG is a versatile tool that can be used to track subcellular proteins through multiple fluorescent techniques. 相似文献
9.
Dr. Carolyn Vargas Prof. Dr. Gerald Radziwill Dr. Gerd Krause Dr. Anne Diehl Prof. Dr. Sandro Keller Dr. Nestor Kamdem Prof. Dr. Constantin Czekelius Annika Kreuchwig Dr. Peter Schmieder Dr. Declan Doyle Prof. Dr. Karin Moelling Dr. Volker Hagen Dr. Markus Schade Prof. Dr. Hartmut Oschkinat 《ChemMedChem》2014,9(7):1458-1462
PDZ (PSD‐95, Dlg, ZO‐1) domains are ubiquitous interaction modules that are involved in many cellular signal transduction pathways. Interference with PDZ‐mediated protein–protein interactions has important implications in disease‐related signaling processes. For this reason, PDZ domains have gained attention as potential targets for inhibitor design and, in the long run, drug development. Herein we report the development of small molecules to probe the function of the PDZ domain from human AF6 (ALL1‐fused gene from chromosome 6), which is an essential component of cell–cell junctions. These compounds bind to AF6 PDZ with substantially higher affinity than the peptide (Ile‐Gln‐Ser‐Val‐Glu‐Val) derived from its natural ligand, EphB2. In intact cells, the compounds inhibit the AF6–Bcr interaction and interfere with epidermal growth factor (EGF)‐dependent signaling. 相似文献
10.
Fluorophore‐Labeled Cyclooxygenase‐2 Inhibitors for the Imaging of Cyclooxygenase‐2 Overexpression in Cancer: Synthesis and Biological Studies
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Dr. Atul Bhardwaj Dr. Jatinder Kaur Dr. Frank Wuest Dr. Edward E. Knaus 《ChemMedChem》2014,9(1):109-116
A group of cyclooxygenase‐2 (COX‐2)‐specific fluorescent cancer biomarkers were synthesized by linking the anti‐inflammatory drugs ibuprofen, (S)‐naproxen, and celecoxib to the 7‐nitrobenzofurazan (NBD) fluorophore. In vitro COX‐1/COX‐2 inhibition studies indicated that all of these fluorescent conjugates are COX‐2 inhibitors (IC50 range: 0.19–23.0 μM ) with an appreciable COX‐2 selectivity index (SI≥4.3–444). In this study the celecoxib–NBD conjugate N‐(2‐((7‐nitrobenzo[c][1,2,5]oxadiazol‐4‐yl)amino)ethyl)‐4‐(5‐(p‐tolyl)‐3‐(trifluoromethyl)‐1H‐pyrazol‐1‐yl)benzenesulfonamide ( 14 ), which displayed the highest COX‐2 inhibitory potency and selectivity (COX‐2 IC50=0.19 μM ; SI=443.6), was identified as an impending COX‐2‐specific biomarker for the fluorescence imaging of cancer using a COX‐2‐expressing human colon cancer cell line (HCA‐7). 相似文献
11.
Zhenshun Li Shaolong Wan Wei Xu Yuntao Wang Bakht Ramin Shah Weiping Jin Yijie Chen Bin Li 《应用聚合物科学杂志》2015,132(17)
Robust and facile strategies are required to fabricate film with high luminescence for application in the fields of biomaterials. In this study, the luminescent electrospinning cellulose fibrous mats were decorated with CdTe quantum dots (QDs) and poly(diallyl dimethyl ammonium chloride) (PDDA) using layer by layer (LBL). The characterizations of the LBL films coated mats were executed by X‐ray photoelectron spectroscopy, Fourier transform infrared spectroscopy, scanning electron microscopy, fluorescent spectroscopy, X‐ray diffraction, thermal gravimetric analysis, and differential scanning calorimetry. The luminescent intensities were linearly increased with adding the amount of deposited bilayers. The green fabricated (QDs/PDDA)n coated mat through physical interactions is a promising luminescent material. © 2015 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2015 , 132, 41893. 相似文献
12.
13.
In this paper, we reported a new method to fabricate CdTe quantum dots (CdTe QDs), which were synthesized in aqueous solution using thioglycolic acid and L ‐phenylalanine (L ‐Phe) as costabilizing agent. Then, they were transferred into organic phase with the assistance of cetyltrimethylammonium bromide (CTAB) for further utilization. Finally, we use toluene diisocyanate as a bridge between CdTe QDs and polyacrylate (CPA), whose side chain has hydroxyl group, to synthesize a fluorescent composite polymer CdTe‐CPA. In addition, we synthesized an organic substance 4‐[2,4‐di(tert‐butyl)]phenoxy‐N‐(2‐hydroxyethyl)‐1,8‐naphthalimide (N), and obtained 1,8‐naphthahmide fluorescent polymer N‐CPA in the same way as it did on CdTe‐CPA. The resulting materials were characterized by Fourier transform infrared spectroscopy (FT‐IR), gel permeation chromatography (GPC), differential scanning calorimetry (DSC), transmission electron microscope (TEM), and photoluminescence (PL). Then, we studied the fluorescence resonance energy transfer (FRET) between CdTe‐CPA and N‐CPA. The data obtained from absorption and fluorescence emission spectral indicated that the FRET from N‐CPA to CdTe‐CPA could be efficiently triggered in the solution of chloroform. © 2012 Wiley Periodicals, Inc. J. Appl. Polym. Sci., 2013 相似文献
14.
CdS Quantum Dot‐Sensitized Vertical TiO2 Nanorod Arrays by a Simple Linker‐Assisted SILAR Method
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Lidong Wei Fen Li Sujuan Hu Haijin Li Bo Chi Jian Pu Li Jian 《Journal of the American Ceramic Society》2015,98(10):3173-3178
The homogeneously deposition of quantum dots (QDs) on the mesoporous or one‐dimensional TiO2 film with a well‐covered layer has become one major challenge in quantum dot‐sensitized solar cell (QDSSC). In this study, one‐dimensional TiO2 nanorod arrays based photoanode prepared by a simple linker‐assisted SILAR method is studied. The influence of the short organic ligand (TGA) on the homogeneity of QDs on TiO2 nanorod arrays is discussed and the photoelectrochemical performance of QDSSC is evaluated by UV–vis absorbance spectroscopy, current–voltage performance and electrochemical impedance spectroscopy. The results show that the TGA ligand can act as an efficient bridge between CdS QDs and TiO2, whereas the content and particle size of the QDs can be easily tuned by controlling the dipping time and SILAR cycle. Owing to the presence of TGA, the charge‐transfer rate at CdS QDs and TiO2 interfacial region is noticeable enhanced. 相似文献
15.
Are quantum dots ready for in vivo imaging in human subjects? 总被引:2,自引:0,他引:2
Nanotechnology has the potential to profoundly transform the nature of cancer diagnosis and cancer patient management in the
future. Over the past decade, quantum dots (QDs) have become one of the fastest growing areas of research in nanotechnology.
QDs are fluorescent semiconductor nanoparticles suitable for multiplexed in vitro and in vivo imaging. Numerous studies on
QDs have resulted in major advancements in QD surface modification, coating, biocompatibility, sensitivity, multiplexing,
targeting specificity, as well as important findings regarding toxicity and applicability. For in vitro applications, QDs
can be used in place of traditional organic fluorescent dyes in virtually any system, outperforming organic dyes in the majority
of cases. In vivo targeted tumor imaging with biocompatible QDs has recently become possible in mouse models. With new advances
in QD technology such as bioluminescence resonance energy transfer, synthesis of smaller size non-Cd based QDs, improved surface
coating and conjugation, and multifunctional probes for multimodality imaging, it is likely that human applications of QDs
will soon be possible in a clinical setting. 相似文献
16.
Anne B. Neef Prof. Dr. Nathan W. Luedtke 《Chembiochem : a European journal of chemical biology》2014,15(6):789-793
Metabolic incorporation of azido nucleoside analogues into living cells can enable sensitive detection of DNA replication through copper(I)‐catalyzed azide–alkyne cycloaddition (CuAAC) and strain‐promoted azide–alkyne cycloaddition (SPAAC) “click” reactions. One major limitation to this approach is the poor chemical stability of nucleoside derivatives containing an aryl azide group. For example, 5‐azido‐2′‐deoxyuridine (AdU) exhibits a 4 h half‐life in water, and it gives little or no detectable labeling of cellular DNA. In contrast, the benzylic azide 5‐(azidomethyl)‐2′‐deoxyuridine (AmdU) is stable in solution at 37 °C, and it gives robust labeling of cellular DNA upon addition of fluorescent alkyne derivatives. In addition to providing the first examples of metabolic incorporation into and imaging of azide groups in cellular DNA, these results highlight the general importance of assessing azide group stability in bioorthogonal chemical reporter strategies. 相似文献
17.
Effect of topological structure on photoluminescence of PbSe quantum dot‐doped borosilicate glasses
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Zhousu Xu Xiaofeng Liu Chun Jiang Dewei Ma Jinjun Ren Cheng Cheng Jianrong Qiu 《Journal of the American Ceramic Society》2018,101(4):1508-1515
Borosilicate glasses doped with PbSe quantum dots (QDs) were prepared by a conventional melt‐quenching process followed by heat treatment, which exhibit good thermal, chemical, and mechanical stabilities, and are amenable to fiber‐drawing. A broad near infrared (NIR) photoluminescence (PL) emission (1070‐1330 nm) band with large full‐width at half‐maximum (FWHM) values (189‐266 nm) and notable Stokes shift (100‐210 nm) was observed, which depended on the B2O3 concentration. The PL lifetime was about 1.42‐2.44 μs, and it showed a clear decrease with increasing the QDs size. The planar [BO3] triangle units forming the two‐dimensional (2D) glass network structure clearly increased with increasing B2O3 concentration, which could accelerate the movement of Pb2+ and Se2? ions and facilitate the growth of PbSe QDs. The tunable broadband NIR PL emission of the PbSe QD‐doped borosilicate glass may find potential application in ultra‐wideband fiber amplifiers. 相似文献
18.
Linear poly(hydroxyethers) (PHEs) were prepared by the base‐induced condensation of bisphenols with epichlorohydrin in a polar mixed solvent. The bisphenols used were 2,2‐bis(4‐hydroxyphenyl)propane (bisphenol A) and 2,2‐bis(4‐hydroxyphenyl)‐hexafluoropropane (bisphenol AF). Bisphenol A–based homo‐PHE (HPHE‐A), bisphenol AF–based homo‐PHE (HPHE‐AF), and copoly(hydroxyethers) (CPHEs) based on both the bisphenols with various compositions were characterized in terms of chemical structure, thermal property, solubility, and contact angle. The incorporation of bisphenol AF unit into HPHE‐A brought about the increases in the glass‐transition temperature, the solubility in organic solvents, and the hydrophobicity. The sequence of the repeating unit in the copolymer was analyzed by 1H–NMR and the result agreed well with the one calculated as a random copolymer. © 2001 John Wiley & Sons, Inc. J Appl Polym Sci 80: 1687–1696, 2001 相似文献
19.
Design,Synthesis, and Evaluation of an 18F‐Labeled Radiotracer Based on Celecoxib–NBD for Positron Emission Tomography (PET) Imaging of Cyclooxygenase‐2 (COX‐2)
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Dr. Jatinder Kaur Ole Tietz Dr. Atul Bhardwaj Alison Marshall Jenilee Way Dr. Melinda Wuest Prof. Frank Wuest 《ChemMedChem》2015,10(10):1635-1640
A series of novel fluorine‐containing cyclooxygenase‐2 (COX‐2) inhibitors was designed and synthesized based on the previously reported fluorescent COX‐2 imaging agent celecoxib–NBD ( 3 ; NBD=7‐nitrobenzofurazan). In vitro COX‐1/COX‐2 inhibitory data show that N‐(4‐fluorobenzyl)‐4‐(5‐p‐tolyl‐3‐trifluoromethylpyrazol‐1‐yl)benzenesulfonamide ( 5 ; IC50=0.36 μM , SI>277) and N‐fluoromethyl‐4‐(5‐p‐tolyl‐3‐trifluoromethylpyrazol‐1‐yl)benzenesulfonamide ( 6 ; IC50=0.24 μM , SI>416) are potent and selective COX‐2 inhibitors. Compound 5 was selected for radiolabeling with the short‐lived positron emitter fluorine‐18 (18F) and evaluated as a positron emission tomography (PET) imaging agent. Radiotracer [18F] 5 was analyzed in vitro and in vivo using human colorectal cancer model HCA‐7. Although radiotracer uptake into COX‐2‐expressing HCA‐7 cells was high, no evidence for COX‐2‐specific binding was found. Radiotracer uptake into HCA‐7 tumors in vivo was low and similar to that of muscle, used as reference tissue. 相似文献
20.
Martine Dhilly Javier Becerril‐Ortega Dr. Nathalie Colloc'h Dr. Eric T. MacKenzie Dr. Louisa Barré Prof. Alain Buisson Dr. Olivier Nicole Dr. Cécile Perrio 《Chembiochem : a European journal of chemical biology》2013,14(6):759-769
GluN2B‐containing NMDA receptors are involved in many important physiological functions and play a pivotal role in mediating pain as well as in several neurodegenerative disorders. We aimed to develop fluorescent probes to target the GluN2B subunit selectively in order to allow better understanding of the relationships between receptor localisation and physiological importance. Ifenprodil, known as the GluNR2B antagonist of reference, was chosen as the template for the elaboration of probes. We had previously reported a fluorescein conjugate that was shown (by confocal microscopy imaging of DS‐red‐labelled cortical neurons) to bind specifically to GluN2B. To elaborate this probe, we explored the influence of both the nature and the attachment point of the spacer between the fluorophore and the parent compound, ifenprodil. We performed chemical modifications of ifenprodil at the benzylic position and on the phenol ring by introducing secondary amine or amide functions and evaluated alkyl chains from two to 20 bonds either including or not including secondary amide functions as spacers. The previously developed probe was found to display the greatest activity in the inhibition of NMDA‐induced Ca2+ influx by calcium imaging experiments on HEK293 cells transfected with the cDNA encoding for GluN1‐1A and GluN2B. Further investigations revealed that this probe had a neuroprotective effect equivalent to that of ifenprodil in a standard test for neurotoxicity. Despite effects of lesser amplitude with these probes relative to ifenprodil, we demonstrated that they displaced [3H]ifenprodil in mouse brain slices in a similar manner. 相似文献