Fabrication and testing of embedded microvalves within PMMA microfluidic devices

The integration of a PDMS membrane within orthogonally placed PMMA microfluidic channels enables the pneumatic actuation of valves within bonded PMMA–PDMS–PMMA multilayer devices. Here, surface functionalization of PMMA substrates via acid catalyzed hydrolysis and air plasma corona treatment were investigated as possible techniques to permanently bond PMMA microfluidic channels to PDMS surfaces. FTIR and water contact angle analysis of functionalized PMMA substrates showed that air plasma corona treatment was most effective in inducing PMMA hydrophilicity. Subsequent fluidic tests showed that air plasma modified and bonded PMMA multilayer devices could withstand fluid leakage at an operational flow rate of 9 μl/min. The pneumatic actuation of the embedded PDMS membrane was observed through optical microscopy and an electrical resistance based technique. PDMS membrane actuation occurred at pneumatic pressures of as low as 10 kPa and complete valving occurred at 14 kPa for ~100 μm by 100 μm channel cross-sections.     

Rapid prototyping of flexible multilayer microfluidic devices using polyester sealing film

Microsystem Technologies - In this study, we proposed a novel fabrication method for rapid prototyping of multilayer flexible microfluidic devices using laser ablated polyester sealing films. The...     

Low-cost,rapid-prototyping of digital microfluidics devices

An innovative and simple microfabrication method for digital microfluidics is presented. In this method, devices are formed from copper substrates or gold compact disks using rapid marker masking to replace photolithography. The new method is capable of forming devices with inter-electrode gaps as small as 50 μm. Saran™ wrap (polyethylene film) and commercial water repellants were used as dielectric and hydrophobic coatings, respectively, to replace commonly used and more expensive materials such as parylene-C and Teflon-AF. Devices formed by the new method enabled single- and two-plate actuation of droplets with volumes of 1–12 μL. Fabricated devices were successfully tested for droplet manipulation, merging and splitting. We anticipate that this fabrication method will bring digital microfluidics within the reach of any laboratory with minimal facilities. Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users.     

Roll manufacturing of flexible microfluidic devices in thin PMMA and COC foils by embossing and lamination

Microfluidics on foil is gaining momentum due to a number of advantages of employing thin films combined with the capability of cost-effective high-volume manufacturing of devices. In this work, ultra-thin, flexible Y-microreactors with microchannels of 100 μm width and 30 μm depth were fabricated in thermoplastic polymer foils. The fluidic pattern was hot roll embossed in 125 μm thick poly-methyl-methacrylate (PMMA) and 130 μm thick cyclic-olefin-copolymer (COC) films using a dry-etched microstructured silicon wafer as a flat embossing tool in a laminator. The sealing of the channels was performed with two different techniques, one based on lamination of SU8 dry film resist (DFR) and the other one based on spin-coated poly-dimethylsiloxane (PDMS). Testing of the interconnected microreactor was carried out using two dye colorant solutions to demonstrate mixing.     

Photolamination bonding for PMMA microfluidic chips

Finding out a rapid and reliable bonding method for plastic microfluidic chips with PCR functions is challenging for analytical chemistry and biochemistry. A rapid, reliable covalent bonding method is introduced for fabricating PMMA PCR biochips with long channels and thin walls. The bonding strength of ~5 MPa was obtained and bulk cohesive failure occurred during tensiling tests. Preliminary leaking tests indicate that photolamination bonding can be implemented readily in the fabrication of PMMA PCR biochips.     

Sample preconcentration in microfluidic devices

Microfluidic systems have attracted considerable attention and have experienced rapid growth in the past two decades due to advantages associated with miniaturization, integration, and automation. Poor detection sensitivities mainly attributed to the small dimensions of these lab-on-a-chip (LOC) devices; however, sometimes can greatly hinder their practical applications in detecting low-abundance analytes, particularly those in bio-samples. Although off-chip sample pretreatment strategies can be used to address this problem prior to analysis, they may introduce contaminants or lead to an undesirable loss of some original sample volume. Moreover, they are often time-consuming and labor-intensive. Toward the goals of automation, improvement in analytical efficiency, and reductions in sample loss and contamination, many on-chip sample preconcentration techniques based on different working principles for improving the detection sensitivity have been developed and implemented in microchips. The aim of this article is to review recent works in microchip-based sample preconcentration techniques and give detailed discussions about these techniques. We start with a brief introduction regarding the importance of preconcentration techniques in microfluidics and the classification of these techniques based on their concentration mechanisms, followed by in-depth discussions of about these techniques. Finally, personal perspectives on microfluidic-based sample preconcentration will be provided. These advancements in microfluidic sample preconcentration techniques may provide promising strategies for improving the detection sensitivities of LOC devices in many practical applications.     

Two-stage polymer embossing of co-planar microfluidic features for microfluidic devices

A two-stage embossing technique for fabricating microchannels for microfluidic devices is presented. A micromachined aluminum mold is used to emboss a polyetherimide (PEI) substrate with a relatively high glass transition temperature (T_g). The embossed PEI is then used as a mold for embossing an amorphous polyethylene terephthalate (APET) substrate with a lower T_g. The resulting APET substrate has the same features as those of the aluminum mold. Successful transfer of features from the aluminum mold to the APET substrate was verified by profilometry, and an application of this method in production of a microfluidic device is presented.     

Particle focusing in microfluidic devices

Focusing particles (both biological and synthetic) into a tight stream is usually a necessary step prior to counting, detecting, and sorting them. The various particle focusing approaches in microfluidic devices may be conveniently classified as sheath flow focusing and sheathless focusing. Sheath flow focusers use one or more sheath fluids to pinch the particle suspension and thus focus the suspended particles. Sheathless focusers typically rely on a force to manipulate particles laterally to their equilibrium positions. This force can be either externally applied or internally induced by channel topology. Therefore, the sheathless particle focusing methods may be further classified as active or passive by the nature of the forces involved. The aim of this article is to introduce and discuss the recent developments in both sheath flow and sheathless particle focusing approaches in microfluidic devices.     

Micro-injection moulding of polymer microfluidic devices

Microfluidic devices have several applications in different fields, such as chemistry, medicine and biotechnology. Many research activities are currently investigating the manufacturing of integrated microfluidic devices on a mass-production scale with relatively low costs. This is especially important for applications where disposable devices are used for medical analysis. Micromoulding of thermoplastic polymers is a developing process with great potential for producing low-cost microfluidic devices. Among different micromoulding techniques, micro-injection moulding is one of the most promising processes suitable for manufacturing polymeric disposable microfluidic devices. This review paper aims at presenting the main significant developments that have been achieved in different aspects of micro-injection moulding of microfluidic devices. Aspects covered include device design, machine capabilities, mould manufacturing, material selection and process parameters. Problems, challenges and potential areas for research are highlighted.     

A multilayer lateral-flow microfluidic device for particle separation

Particle separation technology plays an important role in a wide range of applications as a critical sample preprocessing step for analysis. In this work, we proposed and fabricated a multilayer lateral-flow particle filtration and separation device based on polydimethylsiloxane molding and transfer bonding techniques. Particle separation capability was demonstrated by 4.5-um polystyrene bead filtration and cancer cell (SK-BR-3) retrieving. This device exhibits higher throughput compared with most active particle separation methods and is less vulnerable to membrane clogging problem. This novel multilayer particle filtration and separation device is expected to find applications in biomedical, environmental and microanalysis fields.     

Integrated electrochemical velocimetry for microfluidic devices

We present a new electrochemical velocimetry approach with direct electrical output that is capable of complete device-level integration. The steady reduction rate of a reversible redox species at an embedded microband working electrode is monitored amperometrically. Only one working electrode of arbitrary width is required; all three electrodes, including counter and reference electrodes, are integrated on-chip for complete miniaturization of the sensor. Experimental results are complemented by a theoretical framework including a full 3D electrochemical model as well as empirical mass transfer correlations and scaling laws. When the sensor is operated in the convective/diffusive transport controlled mode, the output signal becomes a predictable function of velocity in two distinct regimes: (i) in the low velocity regime, the signal is directly proportional to flow rate, and (ii) in the high velocity regime, the signal scales as the cube root of the mean velocity. The proposed velocimetry technique is applicable to all practicable pressure-driven laminar flows in microchannels with known cross-sectional geometry.     

Novel no-moving-part valves for microfluidic devices

This study characterizes and analyzes the performances of micro diffusers/nozzles with five types of enhancement structures and one of conventional micro nozzle/diffuser valve. The pressure drops across the designed micro nozzles/diffusers are found to be increased considerably when the obstacle and fin structure are added. Further, the micro nozzle/diffuser having added circular area reveals the lowest pressure drop, owing to the hydraulic diameter is increased by circular area and lower interface friction. The maximum improvement of the loss coefficient ratio is about 16% for an added 3-fin structure operated at a Reynolds number around 70. Upon this situation, the static rectification efficiency improves 4.43 times than the conventional nozzle/diffuser. Experimental results indicate the performance peaks at a Reynolds number around 70, and an appreciable decline is encountered when the Reynolds number is reduced. It is due to the efficiency ratio of conventional micro nozzle/diffuser significant increases with the Reynolds number.     

Digitally tunable microfluidic optical fiber devices

This communication introduces a digital design for tunable microfluidic optical fiber devices. In these systems, multiple, independently controlled microfluidic plugs are pumped into or out of overlap with a fiber structure to modulate its transmission characteristics. The devices described here use eight plugs, eight electrowetting pumps and a corresponding set of molded planar recirculating microchannels to control the depth of the narrowband loss feature associated with a long period fiber grating. Optical measurements illustrate the digital and relatively fast operation of this type of microfluidic fiber device.     

PMMA to Polystyrene bonding for polymer based microfluidic systems

A thermal bonding technique for Poly (methylmethacrylate) (PMMA) to Polystyrene (PS) is presented in this paper. The PMMA to PS bonding was achieved using a thermocompression method, and the bonding strength was carefully characterized. The bonding temperature ranged from 110 to 125 °C with a varying compression force, from 700 to 1,000 N (0.36–0.51 MPa). After the bonding process, two kinds of adhesion quantification methods were used to measure the bonding strength: the double cantilever beam method and the tensile stress method. The results show that the bonding strength increases with a rising bonding temperature and bonding force. The results also indicate that the bonding strength is independent of bonding time. A deep-UV surface treatment method was also provided in this paper to lower the bonding temperature and compression force. Finally, a PMMA to PS bonded microfluidic device was fabricated successfully.     

Application of polydopamine in biomedical microfluidic devices

Polydopamine (PDA) is a bioinspired material with tremendous potential for applications involving surface modifications. By simply immersing the substrate in the dopamine monomer solution, we are able to apply a hydrophilic and biofunctional PDA coating that adheres strongly to any surface, including (super)hydrophobic surface, with unprecedented ease. Using PDA, almost any materials can be immobilized on the surface in a single step by mixing them with the dopamine monomer solution. This review provides a comprehensive coverage of the applications of PDA in the device fabrication, surface modification, and biofunctionalization of biomedical microfluidic devices. While discussing the advantages and limitations of PDA, we pay special attention to its unique properties that specifically benefit biomedical microfluidic devices. We also discuss other potential applications of PDA beyond the current development. Through this review, we hope to promote PDA and encourage a broader adoption of PDA by the microfluidic community.     

Rapid prototyping of shrinkable BOPS-based microfluidic devices

Biaxially oriented polystyrene (BOPS) is a commercialized packaging material, which has the advantages of biocompatibility, non-toxic, transparency, light-weight and cost-effective. Due to the stress accumulated from both directions in plane during the fabrication process, when BOPS was reheated above the glass transition temperature, an isotropic shrinkage will occur. This study proposed a low-cost and rapid prototyping method for the fabrication of BOPS-based microfluidics device. Both laser ablation and micro-milling were used for the fabrication of microchannels on the surface of the BOPS sheet, after thermal induced shrinkage, microchannels with finer microstructure could be achieved. For the sealing of fabricated microchannels on BOPS, two approaches were made using a layer of BOPS or a layer of polyester adhesive film. The thermal induced shrinkage and bonding strength were carefully studied in this study. Several microfluidic devices, including a droplet generator and a diffusion mixer were also fabricated for demonstration. The proposed fabrication method for BOPS-based microfluidics is simple, rapid, cost-effective and without the requirement of cleanroom facility, with help of thermal induced shrinkage, finer structure with high resolution could be achieved with conventional lab tools.     

A low temperature ultrasonic bonding method for PMMA microfluidic chips

Bonding is a bottleneck for mass-production of polymer microfluidic devices. A novel ultrasonic bonding method for rapid and deformation-free bonding of polymethyl methacrylate (PMMA) microfluidic chips is presented in this paper. Convex structures, usually named energy director in ultrasonic welding, were designed and fabricated around micro-channels and reservoirs on the substrates. Under low amplitude ultrasonic vibration, localized heating was generated only on the interface between energy director and cover plate, with peak temperature lower than T _g (glass transition temperature) of PMMA. With the increasing of temperature, solution of PMMA in isopropanol (IPA) increases and bonding was realized between the contacting surfaces of energy director and cover plate while no solution occurs on the surfaces of other part as their lower temperature. PMMA microfluidic chips with micro-channels of 80 μm × 80 μm were successfully bonded with high strength and low dimension loss using this method.     

Modular component design for portable microfluidic devices

A new modular design concept for microfluidic devices is proposed and demonstrated in this study. We designed three key modular microfluidic components: pumps, valves, and reservoirs, and demonstrated that a microfluidic device with specific functions can be easily assembled with those key modular components. Our pumps are man-powerable so that the assembled microfluidic devices require no any other power sources like expensive syringe pumps or air compressors. This feature makes the assembled microfluidic devices completely portable. We also combined our assembled device with other existing mixing microchannels to serve as the mixing and loading system in polymerase chain reaction experiment to amplify DNA successfully. This result shows that those modular components can be integrated into other microchannels, implying great potential applications of the modular design.     

A wise cost-effective supplying bandwidth policy for multilayer wireless cognitive networks

Inventory management is one of the most important research areas in Operations Research and Logistics. It mainly aims to efficiently manage inventories at different facilities (for example, warehouses and plants in Supply Chains (SCs)), minimizing the total cost and satisfying the service levels. Some exact inventories management approaches are successfully proposed and applied to different real scenarios, traditionally related to the SCs, even if the extreme versatility of these techniques could make them attractive to new challenging scenarios such as those related to telecommunications networks. Starting from this vision, the focus of this paper is to show the new benefits of applying an adaptive period inventory management policy to a wireless cognitive telecommunication scenario in which radio transmission resources are treated as short-term life time goods which supplies wisely in order to maximize both economic profit and quality of service offered to wireless users. The system behavior is tested using an agent-based simulator and computational results show that introducing this wise control on the bandwidth supplying mechanism guarantees a more reactive and effective telecommunication network, reaching a good compromise between the total profit and the service levels.     

Inertial particle focusing and spacing control in microfluidic devices

Focusing particles into a tight stream is usually a necessary step prior to counting, detecting and sorting them. Meanwhile, particle spacing control in microfluidic devices could also be applied in the field of accurate cell detection, material synthesis and chemical reaction. To achieve simultaneous particle focusing and spacing control, a novel microchannel composed by Dean and sheath flow section was proposed and fabricated according to the elaborated design principle with its manipulating performance in situ visualized. Using microspheres with a few microns as a template, the trajectory of the particles was discovered to follow lateral migration and reach certain equilibrium positions at the end of the designed Dean section. After being focused, the streamline was further concentrated and centralized with a controllable interparticle distance in sheath flow section. For sheath flow section, the angle between symmetrical tributaries and the mainstream channel and abrupt constriction/expansion structure of mainstream channel as important channel geometric features were investigated to minimize the focusing streamline width and optimize spacing control. An modified analytical model for sheath flow with different tributary angles was derived and proved to well describe the microsphere spacing control process.