应用液相色谱-质谱联用技术同时测定花生中的黄曲霉毒素B1、B2、G1、G2

The aflatoxin B1, B2, G1, G2 in peanut were develop by LC-MS/MS. The mass spectrometer was operated in the positive ion mode using multiple reaction monitoring. The sample was extracted with methanol, and chromatographic separation was carried out on a MGIII-C₁₈ column(2.1 mm×150 mm×1.8 μm) with eluthic sion of V(acetonitrile):V(water)＝35:65. The limit of detection is 0.5 μg•L^－1, the limit of quantitation is 0.5 μg•kg^－1. The linear plots are between 0.5 μg•L^－1 and 25.0 μg•L^－1. Overall recoveries are between 88.3% and 99.2%, and RSD values are between 1.73％ and 3.75％.     

高效液相色谱-串联质谱法测定人血浆中8601、8602、8603的含量

A sensitive liquid chromatography-electrospray ionization-tandem mass spectrometric(LC-MS/MS) method for determination of compound 8601, 8602, 8603 in plasma was developed. The plasma compound 8601, 8602, 8603 was extracted using a solid phase extraction(SPE), and the sample extract was injected onto the LC-MS/MS system. The limit of quantitation(LLOQ) for compound 8601, 8602, 8603 is 0.500 μg•L^－1. This method can be applied to the quantitation compound 8601, 8602, 8603 in plasma.     

LC-MS/MS法定量测定人血浆中TF及其代谢产物M1，M2

A sensitive liquid chromatography-electrospray ionization-tandem mass spectrometric(LC-MS/MS) method for the simultaneous determination of TF and its metabolites M1 and M2 in human plasma were developed and validated. The plasma TF, M1, M2 were extracted using a solid phase extraction(SPE), and the sample extract was injected onto the LC-MS/MS system. The limits of quantitation(LLOQ) for TF, M1, M2 are 1 ng•L^－1. This method allowes the reproducible and accurate quantification with the concentration range of 1-500 ng•L^－1. This method can be applied to the quantitation of TF, M1, M2 in human plasma.     

液相色谱-串联质谱法测定牛奶中舒巴坦和他唑巴坦

A triple quadrupole liquid chromatography tandem mass spectrometry method is presented for the quantitative determination and confirmation of salbactam and tazobactam, the inhibitors of β-lactamase in milk. Milk was extracted with methanol, and the extracts were analyzed by LC-MS/MS in negative mode. The LOQs of salbactam and tazobactam in milk are 10 μg•L^－1 and 100 μg•L^－1, respectively.     

血液中曲马多的HPLC-MS/MS检验方法研究

The method for extraction and determination of tramadol was established by HPLC-MS/MS. Calibration curves are linear on injection of amounts range of 50-1 000 μg•L^－1. The limit of detection is 10 μg•L^－1, and the recovery is above 80% for Tramadol.     

HPLC-ICPMS测定面粉样品中溴酸盐与溴化物

A simple quantitative method of BrO₃^－ and Br^－ in flour using high performance liquid chromatography -inductively coupled plasma mass spectrometry was established. The detection limits for BrO₃^－ and Br^－(counted as Br) were 0.052 µg•L^－1 and 0.048 µg•L^－1, respectively. Three sample preparation methods were compared including ultrasonic method, bathing method and oscillation method. Oscillation method was selected finally. The recoveries of BrO₃^－ and Br^－ are between 93% and 112%.     

简易氢化物发生-ICP-MS法测定高纯钴中痕量的砷

A method for the determination of trace arsenic in high purity cobalt with hydride generation-ICP-MS was reported. Due to the interference of the oxide complex ions ⁵⁹Co¹⁶O^＋, the background concentration of ⁷⁵As is about 100 µg•L^－1 in 1 g•L^－1 cobalt solution, which lead to the impossibility of the direct analysis for the trace As in high purity cobalt. However, the As background concentration can be decreased to several µg•L^－1 if the simple HG system was applied and the 0.05 µg•g^－1 of determination limits can obtained with this method.     

HPLC-MS/MS法测花旗松素在Beagle犬血浆中的浓度及其药代动力学研究

A rapid, sensitive and selective method for the determination of taxifolin in Beagle dog plasma was described by high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). Resveratrol was used as internal standard (I.S.). MS detection and quantitation were done by mass spectrometer using selected reaction monitoring at m/z 303 and 285 [M－H]^－ for taxifolin, and m/z 227 and 185 [M－H]^－ for resveratrol. The calibration curve is linear (r＞0.998) from 2 to 1 000 μg L^－1, and the lower limit of quantification(LLOQ) is 2 μg L^－1.     

液相色谱-串联质谱同时测定牛奶中10种蛋白同化激素的残留

A liquid chromatographic/tandem mass spectrometric (LC/MS/MS) multiresidue method for the simultaneous determination of 10 anabolic steroids (ASs) in whole milk were developed. Milk samples were extracted with methanol and the samples were then subjected to a clean-up procedure using liquid-liquid extraction (LLE) methods. The samples were analysed by LC/MS/MS. The limits of detection (LOD) of LC/MS/MS method used for testing the 10 ASs in whole milk ranged from 0.06 to 0.22 µg•L^－1, and the limits of quantification (LOQ) were from 0.12 to 0.54 µg•L^－1. Experiments on spiked samples of whole milk showed that at addition levels of 1.0, 2.0, 10 µg•L^－1 the average recoveries of the ASs were 24% to 86%, 25% to 91%, 31% to 71%, with relative standard deviations (RSDs) ranged from 12% to 37%, 5% to 20%, 10% to 16%, respectively.     

气相色谱-质谱法测定奶粉中的三聚氰胺

Melamine in milk power was determined by gas chromatography-mass spectrometer(GC/MS) made in china. The results show that the method has a good liner range between 0.005 mg•L^－1 and 2 mg•L^－1, R2 is 0.999 7. The limit of detection is 0.001 mg•L^－1, the quantitative detection limit is 0.02 mg•kg^－1. The recovery for melamine is in the range of 94％-102％. The method has good sensitivity, selectivity and repeatability, which has been applied to the determination of melamine in milk powder.