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对黑曲霉液态发酵产酸性植酸酶的发酵工艺进行了优化实验。通过正交试验进行培养基优化得到最佳配比为:5%可溶性淀粉,5%豆粕,0.9%KCl,0.1%FeSO4·7H2O,0.9%MgSO4·7H2O,0.5%MnSO4。根据极差可知,影响程度,MgSO4·7H2O可溶性淀粉豆粕MnSO4FeSO4·7H2OKCl。通过对发酵条件进行单因素优化得到黑曲霉发酵产植酸酶的最佳发酵条件是:发酵温度30℃,初始pH5.5,摇床转速220r/min,发酵时间60h。在优化条件下,酸性植酸酶酶活力达到了20U。 相似文献
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以啤酒糟为试验材料,研究固态发酵制备阿魏酰低聚糖和膳食纤维的最佳发酵工艺条件。以阿魏酰低聚糖和可溶性膳食纤 维含量为评价指标,选择木聚糖、尿素、磷酸二氢钾为影响因素,通过正交试验确定最佳固态培养基配方。在接种量、发酵时间和发酵 温度3个单因素试验的基础上,利用响应面法优化出芽短梗霉发酵啤酒糟的发酵工艺条件。结果表明,固态发酵的最佳培养基配方为 木聚糖6%、尿素4%和磷酸二氢钾1%;最佳固态发酵条件为接种量12%、发酵时间4 d和发酵温度29 ℃。在此优化条件下,阿魏酰低聚 糖含量和可溶性膳食纤维含量都达到最高,分别为37.67μmol/L和23.76%。 相似文献
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高产耐热型植酸酶菌株ZJ0702发酵条件优化 总被引:1,自引:0,他引:1
目的:对自行分离得到的耐热型植酸酶高产菌株芽孢杆菌ZJ0702发酵条件进行优化,以提高植酸酶的活力.方法:采用单因素试验,研究培养基组分和培养条件对该菌株产植酸酶活力的影响.结果:经优化得到的培养基组分为3.5%麸皮、2%蛋白胨、0.5%硝酸铵、0.01%无机磷、0.2%CaCl2、0.05%KCl、0.03%MgSO4、0.003%FeSO4、0.003%MnSO4、0.03%NaCl;最适培养条件为34℃,接种量7%,pH 7.0,装液量75mL/250mL.在上述培养条件下该菌株发酵72 h产植酸酶活力达到最高值,为11388.4U/mL.结论:与该菌株在原始条件下产酶活力8251 U/mL相比,酶活提高了38%. 相似文献
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固态发酵生产植酸酶的研究 总被引:1,自引:0,他引:1
阐述以AS3.4309为出发菌株,采用紫外线和EB的复合诱变获得植酸酶高产菌AS3.4309-12作为发酵菌株.确定液体种子最佳发酵条件为:发酵温度30℃,摇瓶速度为210 r/rain,装液量为50 mL/250 mL三角瓶,pH5.5,培养基为5%的玉米淀粉和0.5%(NH4)2SO4,孢子接种量2%.确定最佳固态发酵条件:发酵温度30℃,玉米面和麸皮比例4:6,最初pH5.培养基水分51.3%,液体种子接种量5%,发酵时间108 h. 相似文献
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传统固态发酵酿造是多菌系、多酶系、多物系、多变性的多维发酵系统。在整个发酵酿造的酸性环境中,酸性蛋白酶对蛋白水解、微生物生长和风味成分的形成与调控起着举足轻重的作用。以小麦为原料,考察Aspergillus hennebergii产胞外酸性蛋白酶对小麦固态发酵性能的影响。结果表明,与不添加酸性蛋白酶的对照样相比,添加酸性蛋白酶可使小麦固态发酵中蛋白水解度提高28.26%,氨基酸态氮含量提高34.21%,S.cerevisiae生物量提高37.09%,提高总酸36.17%,提高酒醅乙醇含量38.29%;300u以下小麦蛋白水解产物提高达14.1%,可促进芳香族氨基酸代谢,产生更多的风味成分。 相似文献
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以普通芝麻粕为原料,选用枯草芽孢杆菌、乳酸菌、酵母菌多个菌种,通过单因素、正交试验,优化微生物发酵条件,以降低芝麻粕中植酸含量,提高粗蛋白、酸溶蛋白等有益成分的含量。单因素试验的优化条件为:料水比1∶0.8(g∶mL)、R-02与KG-109混菌发酵;正交试验优化的发酵条件为:温度30℃、R-02与KG-109接种比例2∶1、接种量8%、时间10d。在此条件下发酵后植酸含量为0.08%,植酸降解率达到86.21%,粗蛋白含量为49.85%,酸溶蛋白为9.07%,挥发性盐基氮为2 075.5 mg/kg。 相似文献
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A yeast strain producing high levels of phytase was isolated from soil and identified as Candida krusei. The phytase was located on the yeast cell wall and was a glucanase-extractable protein. The phytase production was controlled by the phosphate concentration in the medium used. The maximum production of phytase occurred in a medium containing 0.5 mg of phosphorus per 100 ml, and most of the cells were ellipsoid-shaped and did not exhibit budding. Increasing the concentration of phosphorus in the medium to more than 5 mg of phosphorus per 100 ml caused inhibition of phytase production and 90% of the cells exhibited budding. On the other hand, transferring cells grown in the high-phosphate medium into a phosphate-free one derepressed the phytase production. For example, transferring cells grown in 2 mg of phosphorus per 100 ml into the phosphate-free medium, enhanced the total phytase activity up to 5.5-fold that in the medium containing 0.5 mg of phosphorus per 100 ml. The phytase showed two optimum pHs of 2.5 and 5.5, an optimum temperature of 40 degrees C and the K(m) value for Na-phytate was 0.03 mM. Using in vitro experiments that simulated the conditions of the digestive tract, 50-80% phosphorus was liberated from different plant samples (wheat bran, rice bran and feeds) by the strain. 相似文献
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黑曲霉(Aspergillus niger)产β-葡聚糖酶固态发酵优化的研究 总被引:8,自引:0,他引:8
研究在黑曲霉 (Asp niger) FSN6 5固态发酵中碳氮比、无机氮源、大麦粉添加、水分比例、初始 pH、接种量、培养温度及发酵时间对β 葡聚糖酶酶产量的影响。结果表明 ,培养基中C/N(以麸皮与豆饼粉比例计 )为 8∶1;最佳无机氮源为NH4 NO3;大麦添加对产酶没有明显的诱导作用 ;培养基中最适水分比例为 1∶1;最适发酵条件 :初始发酵pH为 6 0 ;最适接种量为每瓶 0 5mL孢子悬液 (孢子浓度为 4 5× 10 7/mL) ;最适的发酵温度为 33℃ ;在以上最适条件下固态培养 70h ,发酵产酶水平可达 14 16 49u/ g ,优化结果比初始设计提高了 2 6 %。对粗酶酶学特性研究表明 :该酶最适作用 pH为 5 0 ,最适作用温度为 75℃。 相似文献
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为了研究超声波联合酶技术提高大豆分离蛋白(Soybean Protein Isolated,SPI)在酸性条件下(pH 4)乳化性能的效果,本文以大豆分离蛋白为原料,以乳化性能和乳状液粒径为衡量指标,确定超声波联合植酸酶-酸性蛋白酶(Ultrasound combined with phytase-acidic protease,Uphy-aci)改性方法的最适宜条件。研究发现,当SPI浓度6%,植酸酶添加量4 U/g,酸性蛋白酶添加量1500U/g,植酸酶与酸性蛋白酶的酶解时间分别为50 min和30 min时,改性后的SPI(pH 4)乳化性能明显增加,乳状液粒度减小;通过表面疏水性(H0)和扫描电镜(SEM)分析了超声波-酶复合改性处理的SPI,发现在酸性条件下,SPI表面疏水性含量为487.78,比未改性提高了71.2%,并呈现破碎均一、多孔的微观结构。因此,超声波与植酸酶-酸性蛋白酶联合改性提高酸性条件下SPI的乳化特性等功能性质,并且拓宽了大豆分离蛋白的应用领域。 相似文献
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Abstract: In this study, alkaline phytase was added to whole‐wheat bread and the phytate content and mineral profiles were compared to commercially available acidic phytase. At neutral pH, some phytate (approximately 20%) was degraded by endogenous phytase in wheat flour, while 40% of phytate was hydrolyzed by alkaline phytase DS11 and a 35% reduction was observed with acidic phytase. Most of the enzymatic activity occurred during the proofing stage, and the rate of reaction depended on pH. DS11 phytase effectively degraded the phytate level within a 30 min treatment at pH 7; however, at least 60 min was needed with acidic phytase to achieve the same hydrolysis level. Mineral profiles were also dramatically affected by the phytate reduction. The biggest increase was observed in Fe2+ by the phytase treatment. The Fe2+ content increased 10‐fold at pH 7 and 8‐fold at pH 5 with alkaline phytase DS11. Alkaline phytase DS11 was shown to be effective at phytate reduction in whole‐wheat bread preparation. Additionally, phytate degradation enhanced the mineral availability of bread. 相似文献
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在荷叶离褶伞菌丝体摇瓶发酵条件研究结果的基础上,采用均匀设计,在200L发酵罐中,对荷叶离褶伞中试发酵条件与培养基配方进行了优化研究,结果表明荷叶离褶伞菌丝体中试发酵的最佳条件为搅拌转速160r/min、罐压0.2MPa、通气量80L/h、pH值为6.5、温度20℃、接种量10%;最佳培养基配方为玉米面5%、大豆0.5%、ZnSO4 0.025%、MgSO4 0.05%、KH2PO4 0.05%。发酵至第8d菌丝体生物量达到最大(10.578g/L),第9d胞外多糖(exopolysaccharides,EPS)产量最大(1.212g/L)。 相似文献