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1.
Neil J. Mann Stella L. O’Connell Kylie M. Baldwin Indu Singh Barbara J. Meyer 《Lipids》2010,45(8):669-681
Fish are a rich source of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), two long-chain polyunsaturated n-3 fatty
acids (LC n-3 PUFA) with cardiovascular benefits. A related but less-investigated LC n-3 PUFA, docosapentaenoic acid (DPA),
is more common in seal oil and pasture-fed red meats. This study compared indicators of platelet function and plasma lipids
in healthy volunteers given supplements containing these different fatty acids (FA) for 14 days. Subjects, randomised into
three groups of ten, consumed capsules of tuna oil (210 mg EPA, 30 mg DPA, 810 mg DHA), seal oil (340 mg EPA, 230 mg DPA,
450 mg DHA) or placebo (sunola) oil. Supplementary LC n-3 PUFA levels were approximately 1 g/day in both fish and seal oil
groups. Baseline dietary FA and other nutrient intakes were similar in all groups. Both fish and seal oil elevated platelet
DHA levels (P < 0.01). Seal oil also raised platelet DPA and EPA levels (P < 0.01), and decreased p-selectin (P = 0.01), a platelet activation marker negatively associated with DPA (P = 0.03) and EPA (P < 0.01) but not DHA. Plasma triacylglycerol decreased (P = 0.03) and HDL-cholesterol levels increased (P = 0.01) with seal oil only. Hence, seal oil may be more efficient than fish oil at promoting healthy plasma lipid profiles
and lowering thrombotic risk, possibly due to its high DPA as well as EPA content. 相似文献
2.
Valéria T. Crexi Micheli L. Monte Maurício L. Monte Luiz A. A. Pinto 《Journal of the American Oil Chemists' Society》2012,89(2):329-334
The aims of this study were to compare three treatments in the chemical hydrolysis reaction of bleached oil from carp (Cyprinus carpio) heads and to obtain polyunsaturated fatty acid concentrates by urea complexation. The three treatments were carried out
with different oil:ethanol molar ratios. In the treatment with a 1:39 molar ratio, a higher yield of free fatty acids was
found. These fatty acids were submitted to urea complexation (−10 °C for 20 h, and urea–fatty acid ratio of 4.5–1). There
was a 31.4% increase in monounsaturated and polyunsaturated fatty acids (MUFA and PUFA) content and a 75% decrease in saturated
fatty acids (SAF) content. An increase of 85.4% in the EPA + DHA content was found. The non-urea complexing fraction can be
considered a rich source of MUFA and PUFA with a total amount of 88.9%. 相似文献
3.
Arnar Halldorsson Bjorn Kristinsson Gudmundur G. Haraldsson 《European Journal of Lipid Science and Technology》2004,106(2):79-87
The main objective of this study was to compare the fatty acid selectivity of numerous commercially available lipases toward the most ubiquitous fatty acids present in fish oils in form of their corresponding ethyl esters. Special interest was taken in their ability to separate the n‐3 long‐chain polyunsaturated fatty acids (PUFA), mainly eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), from the more saturated fatty acids as well as exploiting the putative discrimination between these highly valuable n‐3 PUFA. Hydrolysis of sardine oil ethyl esters in a Tris buffer solution by 12 microbial lipases is described. The results reveal that all of the lipases strongly discriminate against the n‐3 PUFA and prefer the more saturated fatty acids as substrates. Most of the lipases discriminate between EPA and DHA in favor of EPA, however, 2 bacterial lipases from Pseudomonas were observed to prefer DHA to EPA. Digestive lipolytic enzymes isolated from salmon and rainbow trout intestines displayed reversed fatty acid selectivity when their fish oil triacylglycerol hydrolysis was studied. Thus, the n‐3 PUFA including EPA and DHA were observed to be hydrolyzed at a considerably higher rate than the more saturated fatty acids. 相似文献
4.
Lipase-catalyzed interesterification was used to prepare different structured lipids (SL) from rice bran oil (RBO) by replacing
some of the fatty acids with α-linolenic acid (ALA) from linseed oil (LSO) and n-3 long chain polyunsaturated fatty acids
(PUFA) from cod liver oil (CLO). In one SL, the ALA content was 20% whereas in another the long chain n-3 PUFA content was
10%. Most of the n-3 PUFA were incorporated into the sn-1 and sn-3 positions of triacylglycerol. The influence of SL with RBO rich in ALA and EPA + DHA was studied on various lipid parameters
in experimental animals. Rats fed RBO showed a decrease in total serum cholesterol by 10% when compared to groundnut oil (GNO).
Similarly structured lipids with CLO and LSO significantly decreased total serum cholesterol by 19 and 22% respectively compared
to rice bran oil. The serum TAGs level of rats fed SLs and blended oils were also significantly decreased by 14 and 17% respectively
compared to RBO. Feeding of an n-3 PUFA rich diet resulted in the accumulation of long chain n-3 PUFA in various tissues and
a reduction in the long chain n-6 PUFA. These studies indicate that the incorporation of ALA and EPA + DHA into RBO can offer
health benefits. 相似文献
5.
Leon C. Boyd Mei Fong King Brian Sheldon 《Journal of the American Oil Chemists' Society》1992,69(4):325-330
The stability of unsaturated fatty acids to oxidation was monitored by following gas chromatographic (GC) analyses of headspace
volatiles in comparison to changes in polyunsaturated fatty acids (PUFA) and increases in malonaldehydevia the 2-thiobarbituric (TBA) assay. Pure standards of linoleic acid (Lo) and n-3 fatty acids [eicosapentaenoic (EPA) and docosahexaenoic
acid (DHA)] were added to headspace vials, equilibrated in air for 10 min, followed by heating at 80°C in teflon-capped vials
for different time intervals. Headspace analysis showed increases in acetaldehyde, propenal, and propanal, corresponding to
the oxidation of n-3 fatty acids, whereas hexanal production corresponded to losses of linoleic acid. The analysis of propanal
by GC-headspace after only five minutes of heating appeared to be the most effective method of monitoring the oxidation of
n-3 fatty acids, as indicated by correlations between TBA values and loss of PUFA. The oxidation of Lo, EPA and DHA appeared
to be a function of the number of double bonds. Correlations between PUFA depletion, TBA values and volatile formation indicate
that under the prescribed conditions of this experiment, GC-headspace analysis of propanal and pentane/hexanal is an excellent
method for following the oxidation of selected n-3 fatty acids and linoleic acid. 相似文献
6.
Yuji Shimada Kazuaki Maruyama Masaki Nakamura Suguru Nakayama Akio Sughihara Yoshio Tominaga 《Journal of the American Oil Chemists' Society》1995,72(12):1577-1581
Polyunsaturated fatty acids (PUFA), especially docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA), can be concentrated
in glycerides by hydrolyzing tuna oil withGeotrichum candidum lipase, the main components in the resulting oil being triglycerides. The reaction mechanism of this selective hydrolysis
was investigated. Although the lipase acted well on the esters of oleic, linoleic, and α-linolenic acids, it did not affect
the esters of γ-linolenic acid, arachidonic acid, EPA, and DHA as much. The action of PUFA-glycerides was mono-> di- > triglycerides.
Furthermore, the condensation of PUFA-partial glycerides and PUFA occurred even in the presence of a large amount of water,
and the partial glycerides converted to the triglycerides by transacylation. These results suggested that the PUFA-rich triglycerides
were accumulated in the glyceride fraction by the following mechanism: The PUFA-partial glycerides generated by the hydrolysis
were converted to PUFA-triglycerides by condensation and transacylation reactions. As the PUFA-triglycerides formed were the
poor substrates of lipase, they were accumulated in the reaction mixture. 相似文献
7.
Arun K. Verma V. Rajkumar Ravindra Kumar K. Gururaj Tarun Pal Singh Manoj K. Singh 《European Journal of Lipid Science and Technology》2023,125(10):2300143
This study investigated the effect of supplementing omega fatty acids-rich oil blend, composed of sunflower oil (1.5% and 3.0%), linseed oil (1.5% and 3.0%), and FineXNV1810 (20 g) on the carcass, meat quality, fatty acid profile, and genes (peroxisome proliferator-activated receptor-α, stearoyl-CoA desaturase, acetyl-CoA carboxylase, hydroxy-3-methylglutaryl coenzyme A, and leptin) of Barbari goats. The goat kids (n = 18) were divided into three groups, namely, group A: basal diet; group B: basal diet + oil blend level 1; and group C: basal diet + oil blend level 2, and subjected to the feeding trial for 120 days followed by slaughter and meat quality studies. No treatment effect was recorded in carcass characteristics, pH, water holding capacity, and proximate composition of meat. However, a significant (p < 0.05) treatment effect was observed in cooking loss, lightness, yellowness, and shear force values of meat. There were significant differences (p < 0.05) in linoleic acid, α-linolenic acids, conjugated linoleic acid (CLA), polyunsaturated fatty acids (PUFA), n − 3 and n − 6 PUFA, PUFA/saturated fatty acids and n − 6/n − 3 ratios, and thrombogenic index among groups. An upregulation of the studied genes in the supplemented groups was observed. There were upregulations in the studied genes in the supplemented groups. Practical applications: Goat meat is in great demand the world over, especially in tropical countries, including India, and does not carry any social or religious prohibition. Although goat meat has relatively less fat, consumers express their concern over the presence of undesirable fatty acids. The present study shows that the fatty acid configuration of goat meat can be improved by a dietary supplementation of an oil blend rich in omega fatty acids. The amount of n − 3 PUFA, n − 6 PUFA, and CLA in goat meat was significantly increased due to the dietary oil blend making it healthy for the consumers. Moreover, the dietary oil blend at the studied levels did not significantly affect the growth and meat quality parameters of the goats. Thus, the studied approach can be successfully followed to produce healthier goat meat. 相似文献
8.
Purification of docosahexaenoic acid from tuna oil by a two-step enzymatic method: Hydrolysis and selective esterification 总被引:3,自引:0,他引:3
Yuji Shimada Kazuaki Maruyama Akio Sugihara Shigeru Moriyama Yoshio Tominaga 《Journal of the American Oil Chemists' Society》1997,74(11):1441-1446
Purification of docosahexaenoic acid (DHA) was attempted by a two-step enzymatic method that consisted of hydrolysis of tuna
oil and selective esterification of the resulting free fatty acids (FFA). When more than 60% of tuna oil was hydrolyzed with
Pseudomonas sp. lipase (Lipase-AK), the DHA content in the FFA fraction coincided with its content in the original tuna oil. This lipase
showed stronger activity on the DHA ester than on the eicosapentaenoic acid ester and was suitable for preparation of FFA
rich in DHA. When a mixture of 2.5 g tuna oil, 2.5 g water, and 500 units (U) of Lipase-AK per 1 g of the reaction mixture
was stirred at 40°C for 48 h, 83% of DHA in tuna oil was recovered in the FFA fraction at 79% hydrolysis. These fatty acids
were named tuna-FFA-Ps. Selective esterification was then conducted at 30°C for 20 h by stirring a mixture of 4.0 g of tuna-FFA-Ps/lauryl alcohol (1:2, mol/mol), 1.0 g water, and 1,000 U of Rhizopus delemar lipase. As a result, the DHA content in the unesterified FFA fraction could be raised from 24 to 72 wt% in an 83% yield.
To elevate the DHA content further, the FFA were extracted from the reaction mixture with n-hexane and esterified again under the same conditions. The DHA content was raised to 91 wt% in 88% yield by the repeated
esterification. Because selective esterification of fatty acids with lauryl alcohol proceeded most efficiently in a mixture
that contained 20% water, simultaneous reactions during the esterification were analyzed qualitatively. The fatty acid lauryl
esters (L-FA) generated by the esterification were not hydrolyzed. In addition, L-FA were acidolyzed with linoleic acid, but
not with DHA. These results suggest that lauryl DHA was generated only by esterification. 相似文献
9.
The quality of commercial fish oil products can be difficult to maintain because of the rapid lipid oxidation attributable
to the high number of polyunsaturated fatty acids (PUFA), specifically eicosapentaenoic acid (EPA) and docosahexaenoic acid
(DHA). While it is known that oxidation in fish oil is generally the result of a direct interaction with oxygen and fatty
acid radicals, there are very few studies that investigate the oxidation kinetics of fish oil supplements. This study uses
hydroperoxides, a primary oxidation product, to model the oxidation kinetics of two commercially available fish oil supplements
with different EPA and DHA contents. Pseudo first order kinetics were assumed, and rate constants were determined for temperatures
between 4 and 60 °C. This data was fit to the Arrhenius model, and activation energies (E
a) were determined for each sample. Both E
a agreed with values found in the literature, with the lower PUFA sample having a lower E
a. The oil with a lower PUFA content fit the first-order kinetics model at temperatures ≥20 °C and ≤40 °C, while the higher
PUFA oil demonstrated first-order kinetics at temperatures ≥4 °C and ≤40 °C. When the temperature was raised to 60 °C, the
model no longer applied. This indicates that accelerated testing of fish oil should be conducted at temperatures ≤40 °C. 相似文献
10.
Yukihisa Tanaka Jiro Hirano Tadashi Funada 《Journal of the American Oil Chemists' Society》1992,69(12):1210-1214
In an attempt to concentrate the content of DHA (docosahexaenoic acid) in a glyceride mixture containing triglyceride, diglyceride
and monoglyceride, fish oil was hydrolyzed with six kinds of microbial lipase. After the hydrolysis, free fatty acid was removed
and fatty acid components of the glyceride mixtures were analyzed. When the hydrolysis withCandida cylindracea lipase was 70% complete, the DHA content in the glyceride mixture was three times more than that in the original fish oil.
The EPA (eicosapentaenoic acid) content became almost 70% of the original fish oil. Hydrolysis with other lipases did not
result in an increase in the DHA content in the glyceride mixtures. Hydrolysis of DHA-rich tuna oil (DHA content is about
25%) withCandida cylindracea lipase resulted in 53% DHA in the glyceride mixture. The EPA content, however, remained close to that of the original tuna
oil. In this report, the acyl chain specificity of lipases is evaluated in terms of hydrolysis resistant value (HRV). HRV
is the ratio between the DHA contents in the glyceride mixture of hydrolyzed oil and original oil. HRV clearly indicates differences
in hydrolysis between DHA and other fatty acids (e.g., saturated and monoenoic acids). 相似文献
11.
Huaixia Yin Kevin Mis Solval Jiaqi Huang Peter J. Bechtel Subramaniam Sathivel 《Journal of the American Oil Chemists' Society》2011,88(10):1641-1648
The following four methods were used to extract salmon oil from red salmon heads: RS1 involved a mixture of ground red salmon
heads and water, no heat treatment, and centrifugation; RS2 involved ground red salmon heads (no water added), heat treatment,
and centrifugation; RS3 involved a mixture of ground red salmon heads and water, heat treatment, and centrifugation; and RS4
involved ground red salmon heads, enzymatic hydrolysis, enzyme inactivation by heat and centrifugation. The four extracted
oil samples were evaluated for chemical, thermal, and rheological physical properties. The RS4 process recovered significantly
higher amounts of crude oil from red salmon heads than the other three extraction methods, while containing a higher % of
free fatty acids and higher peroxide values than RS1, RS2, and RS3 oils. Oleic acid, eicosenoic acid, EPA, and DHA were the
predominant fatty acids accounting for about 60% of all unsaturated fatty acids. The RS1, RS2, RS3, and RS4 extractions contained
9.3, 9.05, 9.35, and 9.45% of EPA and 8.8, 8.55, 9.0, and 9.1% of DHA in the oil, respectively. Weight losses of the oils
increased with increasing temperatures between 200 and 500 °C. The % weight losses at 500 °C were 94.50, 94.58, 94.94, and
95.47% for RS2, RS1, RS3, and RS4, respectively. The apparent viscosities of all the oil samples decreased with the increases
in the temperature. The RS1 extract was more viscous (P < 0.05) than those of RS2, RS3, and RS4 between 0 and 25 °C. 相似文献
12.
Lower Efficacy in the Utilization of Dietary ALA as Compared to Preformed EPA + DHA on Long Chain n-3 PUFA Levels in Rats 总被引:1,自引:1,他引:0
Ramaprasad R. Talahalli Baskaran Vallikannan Kari Sambaiah Belur R. Lokesh 《Lipids》2010,45(9):799-808
We made a comparative analysis of the uptake, tissue deposition and conversion of dietary α-linolenic acid (ALA) to its long
chain metabolites eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) with preformed EPA + DHA. Diets containing linseed
oil [with ALA at ~2.5 (4 g/kg diet), 5 (8 g/kg diet), 10 (16 g/kg diet), 25% (40 g/kg diet)] or fish oil [with EPA + DHA at
~1 (1.65 g/kg diet), 2.5 (4.12 g/kg diet), 5% (8.25 g/kg diet)] or groundnut oil without n-3 polyunsaturated fatty acids (n-3
PUFA) were fed to rats for 60 days. ALA and EPA + DHA in serum, liver, heart and brain increased with increments in the dietary
ALA level. When preformed EPA + DHA were fed, the tissue EPA + DHA increased significantly compared to those given ALA. Normalized
values from dietary n-3 PUFA to tissue EPA + DHA indicated that 100 mg of dietary ALA lead to accumulation of EPA + DHA at
2.04, 0.70, 1.91 and 1.64% of total fatty acids respectively in liver, heart, brain and serum. Similarly 100 mg of preformed
dietary EPA + DHA resulted in 25.4, 23.8, 15.9 and 14.9% of total fatty acids in liver, heart, brain and serum respectively.
To maintain a given level of EPA + DHA, the dietary ALA required is 12.5, 33.5, 8.3 and 9.1 times higher than the dietary
EPA + DHA for liver, heart, brain and serum respectively. Hence the efficacy of precursor ALA is lower compared to preformed
EPA + DHA in elevating serum and tissue long chain n-3 PUFA levels. 相似文献
13.
Deirdre Ní Eidhin David O'Beirne 《European Journal of Lipid Science and Technology》2010,112(8):878-886
The effects of blending camelina oil with a number of fish oils on oxidative stability and fishy odour were evaluated. Camelina oil was found to be more stable than tuna oil, ‘omega‐3’ fish oil and salmon oil as indicated by predominantly lower ρ‐anisidine (AV), thiobarbituric acid reactive substances (TBARS) and conjugated triene levels (CT) during storage at 60 °C for 20 days (p < 0.05). Peroxide values (PV) were similar for all oils until Day 13 when values for camelina oil were higher. Values for blends of the fish oils (50, 25, 15, 5%) with camelina oil were generally between those of their respective bulk oils indicating a dilution effect. Camelina oil had a similar odour score (p < 0.05) to sunflower oil (9.2 and 9.6, respectively) indicating, as expected, an absence of fishy odours. In comparison, the fish oils had lower scores of 6.1 to 6.6 (p < 0.05) indicating mild to moderate fishy odours. Odour scores were improved at the 25% fish oil levels (p < 0.05) and were not different to camelina oil at the 15 or 5% levels (p < 0.05). Practical applications: Camelina oil is a potentially important functional food ingredient providing beneficial n‐3 PUFA. Oil extracted from Camelina sativa seeds contains greater than 50% polyunsaturated fatty acids of which 35‐40% is α‐linolenic acid (C18:3ω3, ALA), an essential omega‐3 fatty acid 1 . While EPA and DHA from fish oils are more potent nutritionally, they are less stable than ALA. This work evaluated innovative blends of fish oil with camelina oil for stability and acceptability. The results demonstrate that there is potential for use of blends of camelina oil with fish oils in food products, as the results show some benefits in terms of reduction of fishy odours. Such information could be valuable in relation to formulation of food products containing high levels of n‐3 PUFA from both plant and fish sources. 相似文献
14.
Barbara J. Meyer Brin F. S. Grenyer Trevor Crowe Alice J. Owen Elizabeth M. Grigonis-Deane Peter R. C. Howe 《Lipids》2013,48(9):863-868
The aim of this study was to determine if changes in omega‐3 polyunsaturated fatty acid status following tuna oil supplementation correlated with changes in scores of depression. A total of 95 volunteers receiving treatment for major depression were randomised to consume 8 × 1 g capsules per day of HiDHA (2 g DHA, 0.6 g EPA and 10 mg Vitamin E) or olive oil (placebo) for 16 weeks, whilst undergoing weekly counseling sessions by trained clinical psychologists using a standard empirically validated psychotherapy. Depression status was assessed using the 17 item Hamilton rating scale for depression and the Beck Depression Inventory by a psychodiagnostician who was blind to the treatment. Blood was taken at baseline and 16 weeks (n = 48) for measurement of erythrocyte fatty acids. With HiDHA supplementation, erythrocyte DHA content rose from 4.1 ± 0.2 to 7.9 ± 0.4 % (mean ± SEM, p < 0.001) of total fatty acids but did not change (4.0 ± 0.2 to 4.1 ± 0.2 %) in the olive oil group. The mean changes in scores of depression did not differ significantly between the two groups (?12.2 ± 2.1 for tuna oil and ?14.4 ± 2.3 for olive oil). However, analysis of covariance showed that in the fish oil group there was a significant correlation (r = ?0.51) between the change in erythrocyte DHA and the change in scores of depression (p < 0.05). Further study of the relationship between DHA and depression is warranted. 相似文献
15.
J. L. Guil-Guerrero J. C. López-Martínez M. A. Rincón-Cervera P. Campra-Madrid 《Journal of the American Oil Chemists' Society》2007,84(4):357-361
The fatty acids (FA) eicosapentaenoic acid (20:5ω-3; EPA) and docosahexaenoic acid (22:6ω-3; DHA), which have several health
benefits, have been concentrated from mako shark liver (Isurus oxyrinchus). The process was carried out in one single step, in which fish liver oil was simultaneously extracted, saponified and concentrated.
Additionally, the polyunsaturated fatty acids (PUFA) concentrate was winterized to crystallize the remaining saturated FA,
resulting in a further increase in the concentration of DHA and EPA. Two variables, temperature and water concentration in
the saponification mixture, were optimized to increase the concentration of ω-3 PUFA. Best results were obtained at 12 °C
and 0% water content in the mixture, obtaining 17.8% purity and 77.6% yield of EPA; DHA purity and yield were 33.3 and 82.2%,
respectively. 相似文献
16.
Kriti Bhandari S. P. Chaurasia A. K. Dalai Alok Gupta 《Journal of the American Oil Chemists' Society》2013,90(11):1637-1644
Tuna fish oil contains 25–30 % docosahexaenoic acid (DHA) and is one of the richest sources of DHA. The present paper investigates the enrichment of DHA by selective esterification of fatty acids obtained from hydrolysis of tuna fish oil catalyzed by Rhizopus oryzae lipase (ROL). The fatty acid mixture obtained after hydrolysis of tuna fish oil, referred to as tuna-FFA contained 26 % DHA. For purification/concentration of DHA in free fatty acids, selective esterification of the fatty acid mixtures with butanol was carried out using ROL in a water-organic solvent system. The best reaction parameters found in this study were pH 7, temperature 35 °C, agitation speed 800 rpm and a fatty acid to solvent (iso-octane) ratio of 1:1.32 (w/v). Also, the effects of other parameters such as type of alcohol, type of enzyme, alcohol to fatty acid ratio, enzyme to fatty acid ratio were studied to determine the most suitable reaction conditions. Exactly 76.2 % of tuna-FFA was esterified in 24 h, under the most suitable reaction conditions and the DHA content in the fatty acid fraction rose from 26 to 86.9 % with 80 % recovery of DHA, after selective esterification. The DHA content of fatty acids in butyl esters was found to be 13.6 %. 相似文献
17.
Gloria Fernández-Lorente Carolina Pizarro Dolores López-Vela Lorena Betancor Alfonso V. Carrascosa Benevides Pessela Jose M. Guisan 《Journal of the American Oil Chemists' Society》2011,88(6):819-826
A new assay was designed to measure the release of omega-3 acids [eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA)]
from the hydrolysis of sardine oil by lipases immobilized inside porous supports. A biphasic system was used containing the
fish oil dissolved in the organic phase and the immobilized lipase suspended in the aqueous phase. The assay was optimized
by using a very active derivative of Rhizomucor miehei lipase (RML) adsorbed onto octyl-Sepharose. Standard reaction conditions were: (a) an organic phase composed by 30/70 (v:v)
of oil in cyclohexane, (b) an aqueous phase containing 50 mM methyl-cyclodextrin in 10 mM Tris buffer at pH 7.0. The whole
reaction system was incubated at 25 °C. Under these conditions, up to 2% of the oil is partitioned into the aqueous phase
and most of the 95% of released acids were partitioned into the organic phase. The organic phase was analyzed by RP-HPLC (UV
detection at 215 nm) and even very low concentrations (e.g., 0.05 mM) of released omega-3 fatty acid could be detected with
a precision higher than 99%. Three different lipases adsorbed on octyl-Sepharose were compared: Candida antarctica lipase-fraction B (CALB), Thermomyces lanuginosa lipase (TLL) and RML. The three enzyme derivatives were very active. However, most active and selective towards polyunsaturated
fatty acids (PUFA) versus oleic plus palmitic acids (a fourfold factor) was CALB. On the other hand, the most selective derivatives
towards EPA versus DHA (a 4.5-fold factor) were TLL and RML derivatives. 相似文献
18.
Recent studies suggest that dietary krill oil leads to higher omega-3 polyunsaturated fatty acids (n-3 PUFA) tissue accretion compared to fish oil because the former is rich in n-3 PUFA esterified as phospholipids (PL), while n-3 PUFA in fish oil are primarily esterified as triacylglycerols (TAG). Tissue accretion of the same dietary concentrations of PL- and TAG-docosahexaenoic acid (22:6n-3) (DHA) has not been compared and was the focus of this study. Mice (n = 12/group) were fed either a control diet or one of six DHA (1%, 2%, or 4%) as PL-DHA or TAG-DHA diets for 4 weeks. Compared with the control, DHA concentration in liver, adipose tissue (AT), heart, and eye, but not brain, were significantly higher in mice consuming either PL- or TAG-DHA, but there was no difference in DHA concentration in all tissues between the PL- or TAG-DHA forms. Consumption of PL- and TAG-DHA at all concentrations significantly elevated eicosapentaenoic acid (20:5n-3) (EPA) in all tissues when compared with the control group, while docoshexapentaenoic acid (22:5n-6) (DPA) was significantly higher in all tissues except for the eye and heart. Both DHA forms lowered total omega-6 polyunsaturated fatty acids (n-6 PUFA) in all tissues and total monounsaturated fatty acids (MUFA) in the liver and AT; total saturated fatty acid (SFA) were lowered in the liver but elevated in the AT. An increase in the DHA dose, independent of DHA forms, significantly lowered n-6 PUFA and significantly elevated n-3 PUFA concentration in all tissues. Our results do not support the claim that the PL form of n-3 PUFA leads to higher n-3 PUFA tissue accretion than their TAG form. 相似文献
19.
Betty Mbatia Bo Mattiasson Francis Mulaa Patrick Adlercreutz 《European Journal of Lipid Science and Technology》2011,113(6):717-723
PUFA from oil extracted from Nile perch viscera were enriched by selective enzymatic esterification of the free fatty acids (FFA) or by hydrolysis of ethyl esters of the fatty acids from the oil (FA‐EE). Quantitative analysis was performed using RP‐HPLC coupled to an evaporative light scattering detector (RP‐HPLC‐ELSD). The lipase from Thermomyces lanuginosus discriminated against docosahexaenoic acid (DHA) most, resulting in the highest DHA/DHA‐EE enrichment while lipase from Pseudomonas cepacia discriminated against eicosapentaenoic acid (EPA) most, resulting in the highest EPA/EPA‐EE enrichment. The lipases discriminated between DHA and EPA with a higher selectivity when present as ethyl esters (EE) than when in FFA form. Thus when DHA/EPA were enriched to the same level during esterification and hydrolysis reactions, the DHA‐EE/EPA‐EE recoveries were higher than those of DHA/EPA‐FFA. In reactions catalysed by lipase from T. lanuginosus, at 26 mol% DHA/DHA‐EE, DHA recovery was 76% while that of DHA‐EE was 84%. In reactions catalysed by lipase from P. cepacia, at 11 mol% EPA/EPA‐EE, EPA recovery was 79% while that of EPA‐EE was 92%. Both esterification of FFA and hydrolysis of FA‐EE were more effective for enriching PUFA compared to hydrolysis of the natural oil and are thus attractive process alternatives for the production of products highly enriched in DHA and/or EPA. When there is only one fatty acid residue in each substrate molecule, the full fatty acid selectivity of the lipase can be expressed, which is not the case with triglycerides as substrates. 相似文献
20.
Separation of eicosapentaenoic acid and docosahexaenoic acid in fish oil by kinetic resolution using lipase 总被引:5,自引:0,他引:5
Gudmundur G. Haraldsson Björn Kristinsson 《Journal of the American Oil Chemists' Society》1998,75(11):1551-1556
The objective of this study was to investigate the use of lipases as catalysts for separating eicosapentaenoic acid (EPA)
and docosahexaenoic acid (DHA) in fish oil by kinetic resolution. Transesterification of various fish oil triglycerides with
a stoichiometric amount of ethanol by immobilized Rhizomucor miehei lipase under anhydrous solvent-free conditions resulted in a good separation. When free fatty acids from the various fish
oils were directly esterified with ethanol under similar conditions, greatly improved results were obtained. By this modification,
complications related to regioselectivity of the lipase and nonhomogeneous distribution of EPA and DHA into the various positions
of the triglycerides were avoided. As an example, when tuna oil comprising 6% EPA and 23% DHA was transesterified with ethanol,
65% conversion into ethyl esters was obtained after 24 h. The residual glyceride mixture contained 49% DHA and 6% EPA (8:1),
with 90% DHA recovery into the glyceride mixture and 60% EPA recovery into the ethyl ester product. When the corresponding
tuna oil free fatty acids were directly esterified with ethanol, 68% conversion was obtained after only 8h. The residual free
fatty acids comprised 74% DHA and only 3% EPA (25:1). The recovery of both DHA into the residual free fatty acid fraction
and EPA into the ethyl ester product remained very high, 83 and 87%, respectively. 相似文献