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 共查询到10条相似文献,搜索用时 125 毫秒
1.
采用高效液相色谱-紫外检测器-傅里叶变换离子回旋共振质谱(HPLC-UV-FTICRMS/MSn)技术获得了葛根素及其有关物质的MSn和HRMS数据,分析其可能的裂解机制,推断有关物质的结构,并依据HRMS数据确证了有关物质及其部分碎片的分子组成,最终鉴定葛根素原料药中4种有关物质,其准分子离子峰[M+H]+分别为m/z433.112 95、433.112 92、417.118 01和417.118 01,通过质谱裂解机制研究以及与文献数据的对比,推测它们的结构分别为3’-羟基葛根素、染料木素-8-C-葡萄糖苷、新葛根素B和新葛根素A。  相似文献   

2.
宗晓菲  张慧荣  李博  李丽 《质谱学报》2012,33(6):357-362
利用电喷雾多级串联质谱技术研究了红花提取物中4种色素类成分,即红花红色素、羟基红花黄色素A、红花黄色素A和红花黄色素B。通过多级串联质谱技术,获得4种化合物的分子离子峰,并由此获得分子质量信息;根据一些特征中性碎片的丢失,提出了红花中4种色素类成分的质谱碎裂规律。根据得到的分子质量和碎片信息,并与文献中的已知化合物比较,建立了红花中色素类化合物的快速分析、质谱鉴定的方法。  相似文献   

3.
邓超  张伟  黄挺  张辉  何雅娟  陶红  全灿  李红梅 《质谱学报》2018,39(5):615-622
本实验对苏丹红Ⅱ标准物质候选物中的主要有机杂质进行定性、定量分析,采用高效液相色谱-离子阱-飞行时间串联质谱法(HPLC-IT-TOF MS)和液相色谱-三重四极杆串联质谱法(HPLC-MS/MS)对有机杂质进行定性分析,鉴别出杂质1和杂质2分别为苏丹红Ⅰ和溶剂橙2,推导出杂质3和4的可能分子结构为苏丹红Ⅱ的同分异构体。利用液相色谱外标法测定苏丹红Ⅰ和溶剂橙2的含量分别为0.017%、0.258%,相对标准偏差分别为1.6%、2.5%;采用与主成分苏丹红Ⅱ响应因子一致的方法定量分析杂质3和4,其含量分别为0.012%、0.028%,相对标准偏差分别为7.0%、4.9%。本研究可为苏丹红Ⅱ一级标准物质的研制奠定基础,对苏丹红Ⅱ的准确测定具有重要意义。  相似文献   

4.
The metabolites of perphenazine in human bile were rapid investigated employing UPLC/Q-TOF MSE (where E represents energy) technique. The bile samples were collected after dosing to human and purified through acetonitrile protein precipitation. Data collection was done by UPLC/Q-TOF MSE. According to the molecule formular educed from the measured accurate mass, mass defect filter(MDF) technique and generic dealkylation tool were used to screen the metabolites. Their structures were elicited by comparing the fragmentation patterns between parent drug and metabolites. All the metabolites has chlorine atom isotope cluster. After oral administration of perphenazine to the patient, 29 metabolites including 16 phase I and 13 phase II metabolites are detected in human bile. The major metabolic pathways of perphenazine in human are hydroxylation, N-dealkylation, desaturation, sulfation and glucuronate conjugation.  相似文献   

5.
Triterpene and saponin in Radix ilicis pubescentis were identificated by HPLC-MS. The samlpe was extracted with methanol, and analyzed by HPLC-IT-MS and HPLC-TOF-MS to obtain the positive-ion and negative-ion MS1 and MS2 spectra together with extract molecular weights. Night triterpenoid saponins were identified, and two of them were confirmed by reference substances. The ESI-MS characteristic of triterpene and saponin in Radix ilicis pubescentis was summarized, and the proposed fragmentation mechanism based on ilexgenin A was illustrated.  相似文献   

6.
Cu2+ and Zn2+ ions were reported to be able to induce Aβ aggregation at nearly physiological concentrations in vitro and related to the pathogenesis of Alzheimer’s disease. The smaller peptides including Aβ(1-28) was chosen as the target peptide, which helped define the nature of the interaction of the full length peptide. And we studied the influences of the different conditions including the pH, temperature, apparatus conditions, concentration on the impact of complex of Zn2+, Cu2+ and Aβ. Our findings by the studies of ESI-MS revealed the stoichiometry of 1:1 for the peptide-metal complexes, and to bind up to four cations upon increasing the metal concentration.  相似文献   

7.
MassWorksTM与气相色谱-质谱联用分析洋甘菊精油成分   总被引:1,自引:0,他引:1  
李斌  周围 《质谱学报》2011,32(4):241-245
采用气相色谱-质谱联用(GC/MS)技术,结合MassWorksTM质谱解析软件,对产自甘肃省永登县的洋甘菊(Chamomila recutita L.)精油成分进行分析。对两种不同极性的色谱柱进行分离比较实验,确定了适合于洋甘菊精油分离分析的色谱条件和质谱条件,解析并确定出精油中的35种化合物。MassWorks软件在低分辨率质谱上可对化合物分子质量实现精确测量,为低分辨四极杆质谱确定化合物的元素组成和解析化合物结构提供重要依据,同时也为植物精油的成分分析提供新的技术手段。  相似文献   

8.
王博雅  赵芊  江骥  胡蓓 《质谱学报》2009,30(Z1):107-108
A sensitive liquid chromatography-electrospray ionization-tandem mass spectrometric(LC-MS/MS) method for the determination of CPRC-127 in human plasma was developed and validated. The plasma was extracted using a liquid-liquid phase extraction(LLE), and the sample extract was injected onto the LC-MS/MS system. The limit of quantitation(LLOQ) for CPRC-127 is 0.5 μg•L-1. This method allows the reproducible and accurate quantification with the concentration range of 0.5-1 000 μg•L-1. This method can be applied to the quantitation of CPRC-127 in human plasma.  相似文献   

9.
合成了三类取代哌嗪修饰的新型联苯哌嗪化合物,并对这些化合物进行多级电喷雾 离子阱质谱裂解分析。在一级全扫描质谱中发现,三乙胺与4,4′-二甲氧基-5,6,5′,6′-二次甲二氧基联苯-2-甲酸酯-2′-甲酰基-4-取代哌嗪化合物形成[M+101+H]+。实验还发现,结构中有氟原子存在的联苯-2-甲酸酯-2′-甲酰基-4-取代哌嗪化合物更易与钠离子结合形成加钠的准分子离子[M+Na]+。二级质谱说明,4,4′-二甲氧基-5,6,5′,6′-二次甲二氧基联苯-2-甲酸酯-2′-甲酰基-4-取代哌嗪化合物的哌嗪上取代基的不同,对二级质谱的碎片离子形式有显著的影响。  相似文献   

10.
韩海 《质谱学报》2006,27(1):30-32
哌嗪乙氧基雌酮是一种可望用于防治骨质疏松症的全新合成的雌激素类化合物,本文采用EI-MS、FAB (-) -MS 、ESI-MS三种质谱技术分别对其结构和裂解途径进行了研究。采用FAB(-) -MS,获得m/z 404[M+Na-H]-、382[M]-、381[M-H]-质谱峰;采用EI-MS, 获得m/z 382 M+•;采用ESI-MS, 获得m/z 383[M+H]+。分子离子m/z 382 M+•和主要特征子离子与其结构特征相符,并解释了其中的主要特征子离子,对其结构和裂解途径进行了确证。  相似文献   

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