2012年广西市售婴幼儿食品食源性致病菌监测与分析

目的了解2012年广西市售婴幼儿食品食源性致病菌污染状况,为食品安全预警和食源性疾病预防提供科学依据。方法从广西14个地区的百货商场、超级市场、批发市场和便利店采集婴幼儿食品,按照国标方法进行金黄色葡萄菌、阪崎肠杆菌、蜡样芽胞杆菌检验。结果 1共监测1 939份样品,总检出率为13.56%(263/1 939),其中,阪崎肠杆菌检出率为3.15%,谷类辅助食品检出率为4.19%明显高于配方食品的检出率0.83%(χ2=15.361,P0.05),蜡样芽胞杆菌检出率为10.37%,配方食品检出率为14.12%明显高于谷类辅助食品的检出率8.68%(χ2=13.238,P0.05),金黄色葡萄球菌检出率0.05%;2定量结果:61份阪崎肠杆菌阳性样品中,43份5 MPN/100 g,3份110 MPN/100 g,最大值为240 MPN/100 g;201份蜡样芽胞杆菌阳性样品中,MPN法测得159份100 MPN/g,10份100 MPN/g,最大值为1 100 MPN/g,平板法测得19份100 cfu/g,13份100 cfu/g,最大值为4×107cfu/g;3不同年龄段谷类辅助制品蜡样芽胞杆菌和阪崎肠杆菌检出率范围分别为5.69%~21.05%和3.45%~5.26%,配方食品蜡样芽胞杆菌和阪崎肠杆菌检出率范围分别为11.11%~16.25%和0%~1.37%;4不同产地婴幼儿食品致病菌检出率范围为8.84%~23.47%。结论广西市售婴幼儿食品存在蜡样芽胞杆菌和阪崎肠杆菌污染,应加强企业生产监管,保障婴幼儿食品的安全。     

一起副溶血性弧菌致食源性疾病暴发疫情的流行病学调查

目的查明S饭店群体性食源性疾病暴发的原因,找出可疑食物,防止类似事件再次发生。方法制定病例定义,开展病例搜索,对病例信息进行描述性分析和病例对照研究,并采集病例粪便标本、剩余食物样品等进行实验室检测。结果暴发病例162例,罹患率22.2%(162/730),临床表现主要为腹泻(100%,162/162)、腹痛(96.3%,156/162)、乏力(77.2%,125/162)、恶心(68.5%,111/162)、呕吐(53.1%,86/162),平均潜伏期为12 h,病例对照结果显示扇贝(OR=1.74,95%CI=1.00~3.02)和牛仔骨(OR=2.87,95%CI=1.38~5.99)为可疑危险因素,34份粪便标本、1份甲鱼切块和1份砧板上检出副溶血性弧菌。结论本次事件为一起副溶血性弧菌污染宴席食物导致群体性食源性疾病暴发事件,交叉污染和食品未加工熟透很可能是发病的主要原因,建议餐饮机构要规范操作流程,加强自我监管,防止此类事件再次发生。     

食源性致病微生物阪崎肠杆菌核酸检测试剂盒的研究

<正>食品安全问题一直是社会各界关注的重点。世界卫生组织曾报道称,食源性疾病是受全球关注的卫生问题,更是影响人类健康的重要因素,而病原微生物感染则是导致食源性疾病发生的主要因素。阪崎肠杆菌在2002年被国际食品微生物标准化委员会(ICMSF)确定为食源性致病菌,如被阪崎肠杆菌感染可能会引起婴幼儿尤其是新生儿患病,如脑膜炎、菌血症及坏死性小肠结肠炎等,更有     

利用环介导等温扩增技术对奶粉中阪崎肠杆菌进行检测

利用阪崎肠杆菌16S-23S rDNA间区(ITS)序列,在比较阪崎肠杆菌与其近源株ITS序列的基础上,设计了4条阪崎肠杆菌LAMP检测特异性引物,建立了奶粉中阪崎肠杆菌LAMP检测方法.用15株阪崎肠杆菌,61株近源菌验证试验表明,所建立的LAMP方法准确且灵敏度高.加菌试验表明,奶粉样品中阪崎肠杆菌检测低限为1.2 CFU/100 g.新建的LAMP方法与FDA BAM方法比较试验表明,两种方法的检测结果完全符合.     

IgG-Biotin-Avidin-HRP信号转导探针结合免疫比色法快速检测克罗诺阪崎肠杆菌

本文建立了一种快速检测克罗诺阪崎肠杆菌的新型免疫比色法。用抗体修饰的磁球(MNPs-IgG)作为捕获探针分离、富集克罗诺阪崎肠杆菌,再通过免疫夹心法分别结合生物素标记的克罗诺阪崎肠杆菌抗体(Biotin-IgG)和亲和素标记的辣根过氧化物酶(Avidin-HRP),形成的复合物与底物四甲基联苯胺(3,3′,5,5′-Tetramethylbenzidine,TMB)发生显色反应,经硫酸终止后,有色产物的吸光度值与克罗诺阪崎肠杆菌浓度的对数有良好的线性关系,在103～107CFU/mL范围内,线性方程为y=0.2353x-0.2307。本研究成功设计优化了基于比色法的克罗诺阪崎肠杆菌定量检测新方法,检测限为8.68×10~2CFU/mL(S/N=3),加标样品检出率96%,检测时间不超过2.5 h,为实际应用与商品化提供了理论支撑。     

使用微孔板光谱分析仪和平板计数测定阪崎肠杆菌的生长

本实验采用96孔板培养阪崎肠杆菌,然后由微孔板实时监测阪崎肠杆菌的生长.采用微孔板光谱分析仪和传统的平板方法测定阪崎肠杆菌数量之间具有非常好的相关性(R2=0.92),且可缩短测定时问(由至少24h,缩短为小于10h).阪崎肠杆菌菌株36℃培养末期数量相近,但生长曲线不同.进一步研究17株阪崎肠杆菌菌株42℃生长情况,发现微孔板光谱分析仪方法不仅能快速简便测定生长,且可以一种实时的方式知道阪崎肠杆菌在整个培养过程中的生长情况.研究结果表明,采用该方法可将阪崎肠杆菌菌株根据生长曲线的差异进行表型分类,该表型与阪崎肠杆菌菌株生化反应表型结果一致.     

阪崎肠杆菌的生物学性状与健康危害

阪崎肠杆菌是肠杆菌科的一种,1980年由黄色阴沟肠杆菌更名为阪崎肠杆菌。阪崎肠杆菌能引起严重的新生儿脑膜炎、小肠结肠炎和菌血症,死亡率高达50%以上。目前,微生物学家尚不清楚阪崎肠杆菌的污染来源,但许多病例报告表明婴儿配方粉是目前发现的主要感染渠道。阪崎肠杆菌的生物学性状及其对人群的健康危害受到人们的关注并被报告。     

柳江县市售国产婴幼儿配方粉中阪崎肠杆菌污染监测分析

目的 了解市售国产婴幼儿配方粉中阪崎肠杆菌污染状况,为消费预警提供科学依据。方法 分别按2011、2012年版国家食源性致病菌监测工作手册对市场上11家国内生产的32份婴幼儿配方粉进行检测。结果 32份样品检出2株阪崎肠杆菌,检出率为6.25%。其中婴幼儿配方奶粉中阪崎肠杆菌检出率4.35%(1/23);婴幼儿谷物食品中阪崎肠杆菌检出率11.11%(1/9)。结论 柳江县市售部分婴幼儿配方粉中存在阪崎肠杆菌污染,食用安全隐患不容忽视,应加强监测力度,预防和控制阪崎肠杆菌引起的食物中毒事件发生。     

中国安徽阜阳劣质婴儿配方粉中阪崎肠杆菌的污染

2004年中国安徽阜阳劣质婴儿配方粉事件引起了我国政府的高度重视.为了调查婴儿配方粉中阪崎肠杆菌的污染状况,根据美国FDA和加拿大实验室的方法,建立了婴儿配方粉中阪崎肠杆菌的分离鉴定技术.从87份阜阳劣质奶粉样品中检测到11份阪崎肠杆菌阳性样品,污染阳性率为12.6%.用API 20E和Qualicon BAX(R)系统鉴定了11株阪崎肠杆菌.这是国内首次从婴儿配方粉中分离到阪崎肠杆菌菌株.     

基于PAMAM树枝状大分子纳米免疫传感器快速检验婴幼儿奶粉中的阪崎肠杆菌

以聚酰胺胺树状大分子材料结合还原性石墨烯纳米复合物为电活性修饰膜层,固定于玻碳电极表面,采用物理吸附法固定化辣根过氧化物酶标记阪崎肠杆菌抗体制备成纳米免疫传感器,建立一种快速检测婴幼儿配方奶粉中阪崎肠杆菌的方法。优化的试验条件:以K3[Fe(CN)_6]为探针,过氧化氢浓度为0.5 mmol/L,pH 7.0的PBS溶液为检测底液,孵育温度37℃、孵育时间20 min。采用微分脉冲伏安法研究免疫传感器响应电流与阪崎肠杆菌浓度之间的关系。结果表明,免疫响应电流与阪崎肠杆菌浓度之间呈线性关系(ΔIpc(μA)=-1.1817 lg C+1.723,R~2=0.9989),其检出限为5.8×10~1 CFU/mL(S/N=3)。该法用于检测婴幼儿配方奶粉中的阪崎肠杆菌,其结果与国标方法(实时荧光PCR法)的数据一致,而且具有样品预处理简单、灵敏度高、快速、费用低等优点,可用于婴幼儿食品中阪崎肠杆菌的快速检测。     

Reliable identification of grapevine rootstock varieties using RAPD PCR on woody samples

Since grapevine ( Vitis spp .) rootstock material is being traded increasingly as disbudded woody material a lack of distinctive morphological features on such material necessitates an alternative and reliable means of identification. Methods described here were developed for rapid and efficient extraction of DNA from woody samples rich in phenolic compounds and polysaccharides, and for subsequent identification of varieties by RAPD PCR. Using these methods, and with the application of only one selected RAPD primer, we were able to differentiate sixteen rootstock varieties, including the seven varieties most commonly used in Germany. Problems commonly encountered with reproducibility of RAPD patterns were avoided by choosing primers with a dinucleotide sequence and a high G/C content that allowed a rather high annealing temperature of 45°C. Methods described here should also be useful for other horticultural crops, especially those with woody tissues rich in phenolic compounds and polysaccharides.     

An internet compendium of analytical methods and spectroscopic information for monomers and additives used in food packaging plastics

An internet website (http://cpf.jrc.it/smt/) has been produced as a means of dissemination of methods of analysis and supporting spectroscopic information on monomers and additives used for food contact materials (principally packaging). The site which is aimed primarily at assisting food control laboratories in the European Union contains analytical information on monomers, starting substances and additives used in the manufacture of plastics materials. A searchable index is provided giving PM and CAS numbers for each of 255 substances. For each substance a data sheet gives regulatory information, chemical structures, physico-chemical information and background information on the use of the substance in particular plastics, and the food packaging applications. For monomers and starting substances (155 compounds) the infra-red and mass spectra are provided, and for additives (100 compounds); additionally proton NMR are available for about 50% of the entries. Where analytical methods have been developed for determining these substances as residual amounts in plastics or as trace amounts in food simulants these methods are also on the website. All information is provided in portable document file (PDF) format which means that high quality copies can be readily printed, using freely available Adobe Acrobat Reader software. The website will in future be maintained and up-dated by the European Commission's Joint Research Centre (JRC) as new substances are authorized for use by the European Commission (DG-ENTR formerly DGIII). Where analytical laboratories (food control or other) require reference substances these can be obtained free-ofcharge from a reference collection housed at the JRC and maintained in conjunction with this website compendium.     

Aroma characterization of various apricot varieties using headspace–solid phase microextraction combined with gas chromatography–mass spectrometry and gas chromatography–olfactometry

The characterization of the aromatic profile of several apricot cultivars with molecular tracers in order to obtain objective data concerning the aromatic quality of this fruit was undertaken using headspace–solid phase microextraction (HS–SPME). Six apricot cultivars were selected according to their organoleptic characteristics: Iranien, Orangered, Goldrich, Hargrand, Rouge du Roussillon and A4025. The aromatic intensity of these varieties measured by HS–SPME–Olfactometry were defined and classified according to the presence and the intensity of grassy, fruity and apricot like notes. In the six varieties, 23 common volatile compounds were identified by HS–SPME–GC–MS. Finally, 10 compounds, ethyl acetate, hexyl acetate, limonene, β-cyclocitral, γ-decalactone, 6-methyl-5-hepten-2-one, linalool, β-ionone, menthone and (E)-hexen-2-al were recognized by HS–SPME–GC–O as responsible of the aromatic notes involved in apricot aroma and considered as molecular tracers of apricot aromatic quality which could be utilized to discriminate apricot varieties.     

Tests of potential functional barriers for laminated multilayer food packages. Part I: Low molecular weight permeants

The advent of the functional barrier concept in food packaging has brought with it a requirement for fast tests of permeation through potential barrier materials. In such tests it would be convenient for both foodstuffs and materials below the functional barrier (sub-barrier materials) to be represented by standard simulants. By means of inverse gas chromatography, liquid paraffin spiked with appropriate permeants was considered as a potential simulant of sub-barrier materials based on polypropylene (PP) or similar polyolefins. Experiments were performed to characterize the kinetics of the permeation of low molecular weight model permeants (octene, toluene and isopropanol) from liquid paraffin, through a surrogate potential functional barrier (25 μm-thick oriented PP) into the food simulants olive oil and 3% (w/v) acetic acid. These permeation results were interpreted in terms of three permeation kinetic models regarding the solubility of a particular model permeant in the post-barrier medium (i.e. the food simulant). The results obtained justify the development and evaluation of liquid sub-barrier simulants that would allow flexible yet rigorous testing of new laminated multilayer packaging materials.     

Analysis of benzo[a]pyrene in spiked fatty foods by second derivative synchronous spectrofluorimetry after microwave-assisted treatment of samples

A simple, rapid and inexpensive method has been developed for the determination of benzo[a     

The development of reference materials for paralytic shellfish poisoning toxins in lyophilized mussel. II: Certification study

This paper describes the second part of a project undertaken to develop certified mussel reference materials for paralytic shellfish poisoning toxins. In the first part two interlaboratory studies were undertaken to investigate the performance of the analytical methodology for several PSP toxins, in particular saxitoxin and decarbamoyl-saxitoxin in lyophilized mussels, and to set criteria for the acceptance of results to be applied during the certification exercise. Fifteen laboratories participated in this certification study and were asked to measure saxitoxin and decarbamoyl-saxitoxin in rehydrated lyophilized mussel material and in a saxitoxin-enriched mussel material. The participants were allowed to use a method of their choice but with an extraction procedure to be strictly followed. The study included extra experiments to verify the detection limits for both saxitoxin and decarbamoyl-saxitoxin. Most participants (13 of 15) were able to meet all the criteria set for the certification study. Results for saxitoxin.2HCl yielded a certified mass fraction of <0.07 mg/kg in the rehydrated lyophilized mussels. Results obtained for decarbamoyl-saxitoxin.2HCl yielded a certified mass fraction of 1.59+/-0.20 mg/kg. The results for saxitoxin.2HCl in enriched blank mussel yielded a certified mass fraction of 0.48 +/- 0.06 mg/kg. These certified reference materials for paralytic shellfish poisoning toxins in lyophilized mussel material are the first available for laboratories to test their method for accuracy and performance.     

Announcing a new international peer-reviewed journal:Food Science and Human Wellness now accepting manuscript submission in English

<正>We are pleased to announce the launch of a new international peer-reviewed journal-Food Science and Human Wellness,ISSN 2213-4530,which is an open access journal,produced and hosted by Elsevier B.V.on behalf of Beijing Academy of Food Sciences.Food Science and Human Wellness is an international peer-reviewed English journal that provides a forum for the dissemination of the     

Chinese CTP Market

According to Printing and Printing Equipment Industries Association of China（PEIAC）＇s statistics to the plate manufucturer in China, in 2013, the actual offset plate production has reached 346 million square meters in China. Among them, the CTP production volume was 245 million square meters, up by 11% than that of last year; the total sales of the CTP plate was 239 million square meters, up by 13%.     

Preparatory Work of Print China 2015 is Going Smoothly

正Held at Guangdong Modern International Exhibition Center,Print China 2015 will cover 7exhibition halls,besides the original Hall No.3,4,5,6,7,the newly built F zone of Hall 3 will be used too.The total area will be140,000 square meters.Hall 3:Offset and large printing equipment,package printing equipment,post press