Synthesis of biopolymeric particles loaded with phosphorus and potassium: characterisation and release tests

The authors synthesised nanoparticles (NPs) loaded with P and K from KH₂ PO₄ using gelatin type‐A and type‐B, and sodium alginate as carriers. Using type‐A and type‐B gelatin, quasi‐spherical particles were obtained, with average sizes of 682 and 856 nm, respectively; with sodium alginate, the resulting NPs exhibited spherical shapes and 600 nm particle average size. The authors found an interaction between KH₂ PO₄ and alginate via the hydrogen bonds existent among the carboxylic groups of the carbohydrate and the OH‐groups of the H₂ PO₄ ‐; interactions among gelatin types with the OH‐groups and the H₂ PO₄ ‐ion were also observed. Adding trypsin to the distilled water solutions of the NPs coated with type‐A gelatin increased the concentration of P in the solution by threefold, while increasing that of K increased by 2.6‐fold. Conversely, adding α ‐amylase to the water solutions with sodium alginate increased the P and K concentrations in the solution by nearly 1.3‐ and 1.1‐fold, respectively. Thus, sodium alginate resulted in NPs with smaller sizes and better spherical formations, though with a high polydispersity index and lower release rate of P and K. This low release rate represents an advantage since plants demand nutrients for long periods, and conventional fertilisers display low use efficiency.Inspec keywords: nanofabrication, nanoparticles, hydrogen bonds, gelatin, biomedical materials, particle size, enzymes, molecular biophysics, biochemistry, nanobiotechnology, polymer films, potassium compoundsOther keywords: sodium alginate, biopolymeric particles, release tests, type‐B gelatin, spherical shapes, carboxylic groups, OH‐groups, distilled water solutions, type‐A gelatin, quasi‐spherical particles, particle average size, hydrogen bonds, trypsin, spherical formations, high polydispersity index, plants, α‐amylase, size 682.0 nm, size 856.0 nm, size 600.0 nm, H₂ PO₄ , KH₂ PO₄      

Green synthesis of CuO nanoparticles loaded on the seashell surface using Rumex crispus seeds extract and its catalytic applications for reduction of dyes

In this study, CuO nanoparticles supported on the seashell (CuO NPs/seashell) was prepared using Rumex crispus seeds extract as a chelating and capping agent. The prepared nanocomposite was characterised by Fourier transform infrared spectroscopy, X‐ray diffraction, field emission scanning electron microscopy, energy‐dispersive X‐ray spectroscopy and transmission electron microscopy. The particle size of CuO NPs on the seashell sheets was in the range of 8–60 nm. Catalytic ability of CuO NPs/seashell was investigated for the reduction of 4‐nitrophenol (4‐NP) and Congo red (CR). It was observed that catalyst can be easily recovered and reused several times without any significant loss of catalytic efficiency.Inspec keywords: nanocomposites, nanoparticles, catalysis, dyes, Fourier transform infrared spectra, X‐ray diffraction, field emission electron microscopy, scanning electron microscopy, X‐ray chemical analysis, transmission electron microscopy, particle size, copper compoundsOther keywords: CuO, size 8 nm to 60 nm, Congo red, 4‐nitrophenol, particle size, transmission electron microscopy, energy dispersive X‐ray spectroscopy, field emission scanning electron microscopy, X‐ray diffraction, Fourier transform infrared spectroscopy, nanocomposite, capping agent, chelating agent, dye reduction, catalytic application, Rumex crispus seeds extract, seashell surface, nanoparticles, green synthesis     

Green synthesis of AgCl/Ag3 PO4 nanoparticle using cyanobacteria and assessment of its antibacterial,colorimetric detection of heavy metals and antioxidant properties

In this study, the extract of two strains of cyanobacteria was used for the synthesis of silver nanoparticles (NPs). UV–vis spectroscopy, X‐ray diffraction, dynamic light scattering and field emission scanning electron microscopy (FESEM) analyses were carried out to characterise the NPs. The antioxidant activity and heavy metal detection properties were investigated; moreover, their minimum inhibitory concentration and minimum bactericidal concentration against the multi‐drug resistant bacteria were determined. The most abundant materials in these extracts were carbohydrates, so the biosynthesis of NPs using exopolysaccharide (EPS) was also investigated. The surface plasmon resonance of NPs had a peak at 435 nm and EPS NPs at 350–450 nm. The NPs produced by Nostoc sp. IBRC‐M5064 extract revealed the face‐centred cubic (fcc) structure of AgCl, while NPs of N. pruniforme showed the fcc crystalline structure of Ag₃ PO₄ and AgCl. The FESEM showed the spherical shape of these NPs. The AgCl/Ag₃ PO₄ colloid, in comparison with AgCl, showed better antioxidant activity and antibacterial effect. The heavy metal detection analysis of NPs revealed that the NPs of both stains involved in Hg (NO₃)₂ detection.Inspec keywords: drugs, light scattering, silver, biochemistry, surface plasmon resonance, X‐ray diffraction, silver compounds, antibacterial activity, ultraviolet spectra, nanoparticles, visible spectra, colloids, microorganisms, nanofabrication, field emission scanning electron microscopy, chemical sensors, nanosensorsOther keywords: cyanobacteria, antibacterial detection, colorimetric detection, dynamic light scattering, antioxidant activity, heavy metal detection analysis, silver nanoparticle synthesis, field emission scanning electron microscopy analysis, UV‐visible spectroscopy analysis, X‐ray diffraction analysis, inhibitory concentration, exopolysaccharide, surface plasmon resonance, Nostoc sp. IBRC‐M5064 extract, face‐centred cubic crystalline structure, FESEM, spherical shape, antibacterial effect, multidrug resistant bacteria, wavelength 350.0 nm to 450.0 nm, AgCl‐Ag₃ PO₄ , Ag     

Green fabricated CuO nanobullets via Olea europaea leaf extract shows auspicious antimicrobial potential

In present investigation, copper oxide (CuO) nanostructures have been prepared via green chemistry. Olea europaea leaf extract act as strong chelating agent for tailoring physical as well as bio‐medical characteristics of CuO at the nano‐size. Physical characterisation such as scanning electron microscope analysis depicts the formation of homogenised spherical shape nanoparticles (NPs) with average size of 42 nm. X‐ray diffraction and Fourier transform infrared spectroscopy further confirmed the crystalline pure phase and monoclinic structure. High performance liquid chromatography (HPLC) testing is performed to evaluate the relative concentration of bioactive molecules in the O. europaea leaf extract. From HPLC results capping action of organic molecules around CuO‐NPs is hypothesised. The antimicrobial potency of biosynthesised CuO‐NPs have been evaluated using colony forming unit (CFU) counting assay and disc diffusion method which shows a significant zone of inhibition against bacterial and fungal strains may be highly potential for future antimicrobial pharmaceutics. Furthermore, reduction of various precursors by plant extract will reduce environmental impact over chemical synthesis.Inspec keywords: copper compounds, antibacterial activity, biochemistry, nanoparticles, nanomedicine, biomedical materials, chromatographyOther keywords: CuO, size 42 nm, chemical synthesis, antimicrobial pharmaceutics, bacterial strain, fungal strain, disc diffusion method, colony forming unit counting assay, biosynthesised CuO‐NP, bioactive molecules, high‐performance liquid chromatography testing, monoclinic structure, crystalline pure phase, Fourier transform infrared spectroscopy, X‐ray diffraction, homogenised spherical shape nanoparticles, scanning electron microscope analysis, CuO biomedical characteristics, chelating agent, green fabricated CuO nanobullets, green chemistry, copper oxide nanostructures, antimicrobial potential, Olea europaea leaf extract     

Biogenic green synthesis of monodispersed gum kondagogu (Cochlospermum gossypium) iron nanocomposite material and its application in germination and growth of mung bean (Vigna radiata) as a plant model

An eco‐friendly green and one‐pot synthesis of highly monodispersed iron (Fe) nanoparticles (NPs) by using a natural biopolymer, gum kondagogu (GK) as reducing and capping agent is proposed. The NPs synthesised were characterised by ultra‐violet–visible spectroscopy, transmission electron microscopy, scanning electron microscopy and X‐ray diffraction. As the concentration of gum and time increases, the intensity of NPs formation increased. The NPs were highly monodispersed with uniform circular shapes of 2–6 nm in size. The formed NPs were crystalline in nature which was confirmed by diffraction analysis. The conversion ratio of Fe ionic form to NPs was 21% which was quantified by inductively coupled plasma mass spectroscopy (ICP‐MS). Fe is essential for plant growth and development. A study was conducted to examine the effect of these NPs on the growth of mung bean (Vigna radiata). The radical length and biomass was increased in seeds exposed to Fe NPs than the ions. The uptake of Fe NPs by the sprouts was also quantified by ICP‐MS, in which Fe was more in mung bean seeds exposed to NPs. The α‐amylase activity was increased in the seeds exposed to NPs. The observed increase in the biomass by Fe NPs and seed germination may facilitate its application in the agriculture as an important cost‐effective method for plant growth.Inspec keywords: nanocomposites, nanoparticles, iron, nanobiotechnology, ultraviolet spectra, visible spectra, transmission electron microscopy, scanning electron microscopy, X‐ray diffraction, enzymes, biological techniquesOther keywords: Biogenic green synthesis, monodispersed gum kondagogu, Cochlospermum gossypium, monodispersed gum kondagogu iron nanocomposite material, Vigna radiata, plant model, mung bean growth, mung bean germination, high‐monodispersed iron nanoparticles, natural biopolymer, ultraviolet‐visible spectroscopy, scanning electron microscopy, transmission electron microscopy, X‐ray diffraction, gum concentration, diffraction analysis, ionic‐to‐nanoparticle iron conversion ratio, nanoparticle formation, inductively coupled plasma mass spectroscopy, plant growth, biomass, alpha‐amylase activity, size 2 nm to 6 nm, Fe     

Low‐temperature synthesis of hierarchical structures of copper oxide and their superior biological activity

In this work, the authors report a facile low‐temperature wet‐chemical route to prepare morphology‐tailored hierarchical structures (HS) of copper oxide. The preparation of copper oxide collides was carried out using varying concentrations of copper acetate and a reducing agent at a constant temperature of 50°C. The prepared HS of CuO were characterised by powdered X‐rays diffraction that indicates phase pure having monoclinic structures. The morphology was further confirmed by field‐emission scanning electron microscope. It reveals a difference in shape and size of copper oxide HS by changing the concentration of reactants. In order to evaluate the effect of H₂ O₂ on CuO NPs, the prepared CuO are modified by treatment with H₂ O₂. In general trend, CuOH₂ O₂ collide showed enhanced protein kinase inhibition, antibacterial (maximum zone 16.34 mm against Staphylococcus aureus) and antifungal activities in comparison to unmodified CuO collides. These results reveal that CuO HS exhibit antimicrobial properties and can be used as a potential candidate in pharmaceutical industries.Inspec keywords: molecular biophysics, antibacterial activity, X‐ray diffraction, microorganisms, copper compounds, nanofabrication, nanoparticles, narrow band gap semiconductors, field emission scanning electron microscopy, enzymes, nanomedicine, particle size, semiconductor growthOther keywords: unmodified CuO collides, low‐temperature synthesis, morphology‐tailored hierarchical structures, copper acetate, reducing agent, monoclinic structures, copper oxide HS, CuO NPs, Staphylococcus aureus, biological activity, copper oxide, powdered X‐ray diffraction, field‐emission scanning electron microscopy, facile low‐temperature wet‐chemical method, protein kinase inhibition, antibacterial activity, antifungal activity, antimicrobial properties, pharmaceutical industries, temperature 50.0 degC, CuO     

Postponement growth and antioxidative response of Brassica nigra on CuO and ZnO nanoparticles exposure under soil conditions

Due to unique physiochemical properties, nanoparticles (NPs) have acquired substantial attention in the field of research. However, threats of ecotoxicity and phytotoxicity have limited their biological applications. In this study in vivo experiments were performed to determine the effect of CuO (12.5, 25 and 50 mg/kg) and ZnO (200, 400 and 600 mg/kg) NPs on growth, and antioxidant activities of Brassica nigra. The results showed that CuO NPs did not affect the seed germination while presence of ZnO NPs in the soil generated an inhibitory effect. Both CuO and ZnO NPs positively influenced the growth of stem and other physiological parameters i.e. stem height increased (23%) at 50 mg/kg CuO while root length decreased (up to 44%) with an increase in the concentration of NPs. Phytochemical screening of apical, middle and basal leaves showed elevated phenolic and flavonoid contents in the range of 15.3–59 μg Gallic Acid Equivalent (GAE)/mg Dry Weight (DW) and 10–35 μg Querceitin Equivalent (QE)/mg DW, respectively, in NPs‐treated plants. Antioxidant activity was higher in CuO NPs‐treated plants as compared to ZnO and control plants. Results conclude that CuO and ZnO NPs at low concentrations can be exploited as nanofertilisers in agriculture fields.Inspec keywords: biochemistry, enzymes, renewable materials, crops, nanoparticles, soil, nanofabrication, zinc compounds, organic compounds, agricultural products, toxicology, nanobiotechnologyOther keywords: antioxidative response, ZnO nanoparticles exposure, soil conditions, unique physiochemical properties, germination, antioxidant activities, brassica nigra plant, antioxidant activity, CuO NP‐treated plants, control plants, ZnO NPs effect, mass 15.3 mug to 59.0 mug, mass 10.0 mug to 35.0 mug, CuO, ZnO     

Development and characterisation of novel Ce‐doped hydroxyapatite–Fe3 O4 nanocomposites and their in vitro biological evaluations for biomedical applications

Hydroxyapatite (HAP: Ca₁₀ (PO₄)₆ (OH)₂) is extensively used in biomedical field because of its biocompatibility, osteoconductivity and non‐toxicity properties. However, HAP exhibits poor mechanical strength and bacterial restriction behavior. To overcome these drawbacks, various metal ions such as Ag⁺, Zn²⁺, Cu²⁺, Ti⁴⁺ and Ce^4+/3+ are incorporated in HAP matrix to increase the mechanical and biological properties. Among these, Cerium (Ce) is selected as antibacterial agent due to its high thermal stability and its applications in dental fillings, bone healing and catheters. Fe₃ O₄ nanoparticles were used in hyperthermia treatment, magnetic fluid recordings and catalysis. In this present study, we have synthesized nanocomposites consisting of 1.25% Ce doped HAP with various concentrations of Fe₃ O₄ NPs as 90:10 (C‐1), 70:30 (C‐2) and 50:50 wt% (C‐3) using ball milling technique. The obtained Ce@HAP‐Fe₃ O₄ nanocomposites were characterized by ATR‐FTIR, XRD, VSM, SEM‐EDAX and TEM analysis. Further, the fabricated Ce@HAP‐Fe₃ O₄ nanocomposites were tested for its antibacterial activity towards Staphylococcus aureus (S. aureus) and Escherichia coli (E.coli), where C‐3 composites exhibit the excellent pathogen inhibition towards E.coli. In addition, the cytotoxicity evaluation on C‐3 nanocomposites by in vitro biocompatibility study using MG‐63 cells shows the prominent viable cell enhancement up to 400µg/mL concentrations.Inspec keywords: nanocomposites, iron compounds, calcium compounds, cerium, mechanical strength, antibacterial activity, biomedical materials, dentistry, bone, nanoparticles, nanofabrication, ball milling, Fourier transform infrared spectra, attenuated total reflection, X‐ray diffraction, magnetometry, scanning electron microscopy, transmission electron microscopy, microorganisms, cellular biophysics, nanomedicineOther keywords: Ce‐doped HAP–Fe3O4 nanocomposite, hydroxyapatite, in vitro biological evaluation, mechanical strength, bacterial restriction behaviour, metal ion, silver ion, zinc ion, copper ion, titanium ion, cerium ion, HAP matrix, antibacterial agent, thermal stability, dental filling, bone healing, catheter, Fe3O4 nanoparticle, hyperthermia treatment, magnetic fluid recording, catalysis, ball milling technique, Fourier transform infrared spectroscopy, attenuated total reflectance spectroscopy, X‐ray diffraction, vibrating sample magnetometry, scanning electron microscopy, SEM‐energy dispersive spectroscopy, transmission electron microscopy, TEM analysis, antibacterial activity, Staphylococcus aureus, Escherichia coli, pathogen inhibition, in vitro biocompatibility, MG‐63 osteoblast cell, cell enhancement, Ca₅ (PO₄)₃ (OH):Ce, Fe₃ O₄      

Green synthesis of Ce2 O3 NPs and determination of its antioxidant activity

In this study, the authors presented synthesis of ceria nanoparticles (NPs) by the bio‐reduction method and their antioxidative activity. Aqueous extract of Euphorbia (Euphorbia amygdaloides) was used as reducing and stabilising agents. They used aqueous extract of Euphorbia (E. amygdaloides) as reducing and stabilising agent. Ultraviolet–visible (UV–vis) absorption spectroscopy was used to monitor the quantitative formation of ceria NPs. They also addressed the characteristics of the obtained ceria NPs using scanning electron microscopy (SEM), X‐ray diffraction (XRD) and transmitting electron microscope (TEM). The synthesised cerium (III) oxide (Ce₂ O₃) NPs were initially noted through visual colour change from colourless pale yellow cerium (III) to light yellow cerium (IV) and further confirmed the band at 345 nm employing UV–vis spectroscopy. The average diameter of the prepared NPs was about 8.6–10.5 nm. In addition, the synthesised Ce₂ O₃ NPs were tested for antioxidant and anti‐bacterial activities using ferric reducing antioxidant power, cupric reducing antioxidant capacity, ferrous ions chelating activity, superoxide the anion radical scavenging and 2, 2′‐azinobis 3‐ethylbenzothiazol to‐6‐sulphonic acid scavenging activity. It could be concluded that Euphorbia (E. amygdaloides) extract can be used efficiently in the production of potential antioxidant and anti‐bacterial Ce₂ O₃ NPs for commercial applications.Inspec keywords: cerium compounds, nanoparticles, nanofabrication, X‐ray diffraction, scanning electron microscopy, transmission electron microscopy, ultraviolet spectra, visible spectraOther keywords: Ce₂ O₃ , α‐tocopherol, butylated hydroxytoluene, anion radical scavenging, ferrous ions chelating activity, 2, 2''‐azinobis 3‐ethylbenzothiazol to‐6‐sulphonic acid scavenging activity, UV‐vis spectroscopy, light yellow cerium, ultraviolet‐visible absorption spectroscopy, SEM, XRD, green synthesis, transmitting electron microscopy, X‐ray diffraction, scanning electron microscopy, aqueous extract Euphorbia amygdaloides, antioxidative activity, bio‐reduction method, ceria nanoparticles, antioxidant activity     

Green synthesis of titanium dioxide nanoparticles with volatile oil of Eugenia caryophyllata for enhanced antimicrobial activities

Different chemo‐physical methods are used to synthesise titanium oxide nanoparticles (TiO₂ NPs), which are often expensive, unfriendly to the environment, toxic, not biocompatible, with a small yield. To resolve these problems, the researchers use green procedures to synthesise TiO₂ ‐NPs by plant extracts of Capsicum annum L. and Allium cepa (onion) and characterise using atomic force microscopy, scanning electron microscopy, transmission electronic microscopy, X‐ray diffraction, ultraviolet (UV)–visible (Vis) spectra and Fourier transform infrared spectroscopy. The results indicate that most NPs synthesised by the first and second procedures of onion had an average diameter of 95.7 and 89.1 nm, while NPs synthesised by C. annum had an average diameter of 103.60 and 90.07 nm, respectively. In UV–Vis spectra, strong absorption was below 470 nm, and energy gap was 3.3 eV in each of the first procedure of A. cepa and the second procedure of C. annum compared with 270 nm, 6.3 eV for each of the second procedure of A. cepa and the first procedure of C. annum. The antimicrobial activities of NPs were evaluated and an attempt was made to enhance these activities by Eugenia caryophyllata plant''s oil in combination therapies. There were synergistic effects between NPs and plant''s oil.Inspec keywords: scanning electron microscopy, visible spectra, nanofabrication, titanium compounds, ultraviolet spectra, X‐ray diffraction, nanoparticles, atomic force microscopy, antibacterial activity, transmission electron microscopy, Fourier transform infrared spectra, nanomedicine, semiconductor materials, semiconductor growthOther keywords: cepa, green synthesis, titanium dioxide nanoparticles, volatile oil, eugenia caryophyllata, capsicum annum, atomic force microscopy, electron microscopy, transmission electronic microscopy, UV‐visible spectra, plant extracts, antimicrobial activities, chemophysical methods, Capsicum annum L., Allium cepa, scanning electron microscopy, X‐ray diffraction, Fourier transform infrared spectroscopy, onion, plant oil, TiO₂      

Cytotoxicity,leishmanicidal, and antioxidant activity of biosynthesised zinc sulphide nanoparticles using Phoenix dactylifera

The synthesis of zinc sulphide nanoparticles (ZnS NPs) using a green approach was explored. The resulting nanoparticles (NPs) were characterised by UV–vis spectroscopy, scanning and transmission electron microscopy, X‐ray diffraction and Fourier transform infrared spectroscopy. The leishmanicidal, cytotoxic and antioxidant activity of the resulting synthesised ZnS NPs (<70 nm) were evaluated against Leishmania major (L. major) promastigotes and amastigotes by MTT assay and using a macrophage model. The ZnS NPs were able to counteract the effects of oxidative metabolites as demonstrated by the oxidant activity. The IC₅₀ value of butylated hydroxyanisole was 26.04 µg/ml as compared with the IC₅₀ for ZnS NPs (90.95 µg/ml). The NPs displayed no cytotoxicity for the murine macrophaghes as the selectivity index (SI) fell into the safety range (SI ≥ 10). These nanomaterials exhibited good antileishmanial activity against the L. major stages that were comparable to that of Glucantime, the drug of choice. The IC₅₀ values of ZnS NPs and Glucantime against amastigotes were 11.59 ± 2.51 and 4.95 ± 2.51 μg/ml, respectively. The IC₅₀ values for ZnS NPs and Glucantime versus promastigote were 29.81 ± 3.15 and 14.75 ± 4.05 μg/ml, respectively. Further investigation is essential to explore the biological effects of ZnS NPs on animal and/or clinical models.Inspec keywords: nanoparticles, nanofabrication, microorganisms, antibacterial activity, ultraviolet spectra, visible spectra, nanobiotechnology, X‐ray diffraction, Fourier transform infrared spectra, zinc compoundsOther keywords: cytotoxicity, leishmanicidal activity, antioxidant activity, biosynthesised zinc sulphide nanoparticles, Phoenix dactylifera, green approach, UV–vis spectroscopy, scanning electron microscopy, transmission electron microscopy, X‐ray diffraction, Fourier transform infrared spectroscopy, Leishmania major promastigotes, Leishmania major amastigotes, MTT assay, macrophage model, oxidative metabolites, butylated hydroxyanisole, murine macrophaghes, selectivity index, glucantime, ZnS     

Green synthesis of copper oxide nanoparticles using aqueous extract of Convolvulus percicus L. as reusable catalysts in cross‐ coupling reactions and their antibacterial activity

CuO nanoparticles (NPs) were prepared by Convolvulus percicus leaves extract as a reducing and stabilising agent. The green synthesised copper oxide NPs was characterised by transmission electron microscope, energy dispersive X‐Ray spectroscopy, X‐ray diffraction, Fourier transform infrared and ultraviolet‐visible analysis. The activities of the CuO NPs as catalyst were tested in the formation of C‐N and C‐O bonds. The N ‐arylated and O ‐arylated products of amides, N‐H heterocycles and phenols were obtained in excellent yields. Furthermore, the separation and recovery of copper oxide NPs was very simple, effective and economical. The recovered catalyst can be reused several times without significant loss of its catalytic activity. Moreover, the antibacterial activity of these NPs was tested against two human pathogenic microbes and showed significant antimicrobial activity against these pathogenic bacteria.Inspec keywords: copper compounds, nanoparticles, nanomedicine, antibacterial activity, biomedical materials, nanofabrication, microorganisms, catalysts, transmission electron microscopy, X‐ray chemical analysis, X‐ray diffraction, Fourier transform spectra, infrared spectra, ultraviolet spectra, visible spectra, catalysisOther keywords: green synthesis, copper oxide nanoparticles, Convolvulus percicus L. aqueous extract, reusable catalysts, cross‐coupling reactions, antibacterial activity, reducing agent, stabilising agent, transmission electron microscope, energy dispersive X‐ray spectroscopy, X‐ray diffraction, Fourier transform infrared spectra, ultraviolet‐visible spectra, C‐N bonds, C‐O bonds, N‐arylated products, O‐arylated products, amides, N‐H heterocycles, phenols, catalytic activity, human pathogenic microbes, antimicrobial activity, CuO     

Bio‐green synthesis ZnO‐NPs in Brassica napus pollen extract: biosynthesis,antioxidant, cytotoxicity and pro‐apoptotic properties

The bio‐green methods of synthesis nanoparticles (NPs) have advantages over chemo‐physical procedures due to cost‐effective and ecofriendly products. The goal of current investigation is biosynthesis of zinc oxide NPs (ZnO‐NPs) and evaluation of their biological assessment. Water extract of Brassica napus pollen [rapeseed (RP)] prepared and used for the synthesis of ZnO‐NPs and synthesised ZnO‐NP characterised using ultraviolet–visible, X‐ray diffraction, Fourier‐transform infrared spectroscopy, field emission scanning electron microscope and transmission electron microscope. Antioxidant properties of ZnO‐NPs, cytotoxic and pro‐apoptotic potentials of NPs were also evaluated. The results showed that ZnO‐NPs have a hexagonal shape with 26 nm size. ZnO‐NPs synthesised in RP (RP/ZnO‐NPs) exhibited the good antioxidant potential compared with the butylated hydroxyanisole as a positive control. These NPs showed the cytotoxic effects against breast cancer cells (M.D. Anderson‐Metastasis Breast cancer (MDA‐MB)) with IC₅₀ about 1, 6 and 6 μg/ml after 24, 48 and 72 h of exposure, respectively. RP/ZnO‐NPs were found effective in increasing the expression of catalase enzyme, the enzyme involved in antioxidants properties of the cells. Bio‐green synthesised RP/ZnO‐NPs showed antioxidant and cytotoxic properties. The results of the present study support the advantages of using the bio‐green procedure for the synthesis of NPs as an antioxidant and as anti‐cancer agents.Inspec keywords: II‐VI semiconductors, wide band gap semiconductors, ultraviolet spectra, toxicology, X‐ray diffraction, biochemistry, zinc compounds, nanomedicine, enzymes, biomedical materials, particle size, antibacterial activity, transmission electron microscopy, molecular biophysics, visible spectra, nanofabrication, cellular biophysics, nanoparticles, cancer, field emission scanning electron microscopy, Fourier transform infrared spectra, semiconductor growthOther keywords: bio‐green synthesis ZnO‐NPs, zinc oxide NPs, synthesised ZnO‐NP, field emission scanning electron microscope, transmission electron microscope, antioxidant properties, bio‐green synthesised RP‐ZnO‐NPs, Fourier‐transform infrared spectroscopy, X‐ray diffraction, breast cancer cells MDA‐MB, pro‐apoptotic potentials, cytotoxic effects, catalase enzyme, bio‐green procedure, time 48.0 hour, time 72.0 hour, size 26.0 nm, time 24.0 hour, ZnO     

Green synthesis of copper oxide nanoparticles and mosquito larvicidal activity against dengue,zika and chikungunya causing vector Aedes aegypti

In the present study, high purity copper oxide nanoparticles (NPs) were synthesised using Tridax procumbens leaf extract. Green syntheses of nano‐mosquitocides rely on plant compounds as reducing and stabilising agents. Copper oxide NPs were characterised using X‐ray diffraction (XRD) analysis, Fourier transform infrared (FT‐IR), Field‐emission scanning electron microscopy with energy dispersive spectroscopy, Ultraviolet–visible spectrophotometry and fluorescence spectroscopy. XRD studies of the NPs indicate crystalline nature which was perfectly matching with a monoclinic structure of bulk CuO with an average crystallite size of 16 nm. Formation of copper oxide NPs was confirmed by FT‐IR studies and photoluminescence spectra with emission peaks at 331, 411 and 433 nm were assigned to a near‐band‐edge emission band of CuO in the UV, violet and blue region. Gas chromatography–mass spectrometry studies inferred the phytochemical constituents of the leaf extract. Larvicidal activity of synthesised NPs using T. procumbens leaf extract was tested against Aedes aegypti species (dengue, chikungunya, zika and yellow fever transmit vector).Inspec keywords: photoluminescence, spectrophotometry, thermal analysis, chromatography, nanoparticles, antibacterial activity, field emission electron microscopy, microorganisms, wide band gap semiconductors, scanning electron microscopy, X‐ray diffraction, copper compounds, ultraviolet spectra, nanofabrication, X‐ray chemical analysis, crystallites, visible spectra, field emission scanning electron microscopy, nanobiotechnology, semiconductor materials, semiconductor growth, fluorescence, mass spectraOther keywords: energy dispersive spectroscopy, ultraviolet–visual spectrophotometry, fluorescence spectroscopy, chikungunya, green synthesis, mosquito larvicidal activity, zika, X‐ray diffraction analysis, field‐emission scanning electron microscopy, XRD, gas chromatography–mass spectrometry, copper oxide nanoparticles, dengue, tridax procumben leaf extract, nanomosquitocides, FTIR, monoclinic structure, crystallite size, photoluminescence spectra, near‐band‐edge emission band, phytochemical constituents, Aedes aegypti species, yellow fever transmit vector, CuO     

Label‐free and dynamic monitoring of cytotoxicity to the blood–brain barrier cells treated with nanometre copper oxide

A cytotoxicity study was conducted with a primary culture of the nervous system cells, including brain microvascular endothelial cells (BMECs) and astrocytes, which are important components of the blood–brain barrier. The real‐time cell analysis (RTCA) was used to determine the cytotoxicity of copper‐oxide nanoparticles (CuO NPs). The IC₅₀ values of CuO NPs in astrocytes and BMECs were determined by the RTCA at different exposure times and were used as base values for further research. DNA damage after exposure to CuO NPs for 3 and 24 h was assessed using comet assay at the IC₅₀ obtained from RTCA. The onset time of cytotoxicity induced by CuO NPs was 2 and 2–4 h post‐exposure in BMECs and astrocytes, respectively. Furthermore, the degree of cytotoxicity induced by exposure to CuO NPs for 24–48 h in the BMECs and astrocytes was similar. Treatment with CuO NPs at 1/2*IC₅₀ and 1/5*IC₅₀ for 3 h induced genotoxicity in both cells as assessed by a measurement of DNA damage, although no cytotoxicity was observed. However, significant DNA damage was observed at all concentrations of CuO NPs used in this study, when the treatment time was 24 h.Inspec keywords: biochemistry, blood, brain, cellular biophysics, copper compounds, DNA, molecular biophysics, nanoparticles, toxicologyOther keywords: label‐free cytotoxicity monitoring, dynamic cytotoxicity monitoring, blood‐brain barrier cells, nervous system cells, brain microvascular endothelial cells, astrocytes, real‐time cell analysis, copper‐oxide nanoparticles, comet assay, genotoxicity, DNA damage measurement, time 24 h to 48 h, time 2 h to 4 h, CuO     

Targeting microbial biofilms: by Arctium lappa l. synthesised biocompatible CeO2 ‐NPs encapsulated in nano‐chitosan

This study is planned to synthesise new biocompatible, nano antimicrobial formulation against biofilm producing strains. Aqueous root extract of Arctium lappa l. was used to synthesise ceria nanoparticles (CeO₂ ‐NPs). The synthesised nanoparticles were encapsulated with nano‐chitosan by sol–gel method and characterised using standard techniques. Gas chromatography‐mass spectrometer of Arctium lappa l. revealed the presence of ethanol, acetone, 1‐ propanol, 2‐methylethane, 1,1‐di‐ethoxy, 1‐Butanol, and oleic acid acted as reducing and surface stabilising agents for tailoring morphology of CeO₂ ‐NPs. Erythrocyte integrity after treatment with synthesised nanomaterials was evaluated by spectrophotometer measurement of haemoglobin release having biocompatibility. Scanning electron microscopy revealed the formation of mono dispersed beads shaped particles with mean particle size of 26.2 nm. X‐ray diffractometry revealed cubic crystalline structure having size of 28.0 nm. After encapsulation by nano‐chitosan, the size of CeO₂ ‐NPs enhances to 48.8 nm making average coverage of about 22.6 nm. The synthesised nanomaterials were found effective to disrupt biofilm of S. aureus and P. aeruginosa. Interestingly, encapsulated CeO₂ ‐NPs revealed powerful antibacterial and biofilm disruption activity examined by fluorescent live/dead staining using confocal laser scanning microscopy. The superior antibacterial activities exposed by encapsulated CeO₂ ‐NPs lead to the conclusion that they could be useful for controlling biofilm producing multidrug resistance pathogens.Inspec keywords: particle size, microorganisms, organic compounds, nanomedicine, sol‐gel processing, cellular biophysics, scanning electron microscopy, optical microscopy, nanoparticles, antibacterial activity, fluorescence, biomedical materials, nanofabrication, X‐ray diffraction, chromatography, filled polymers, cerium compoundsOther keywords: microbial biofilms, aqueous root extract, sol–gel method, gas chromatography‐mass spectrometer, 1‐di‐ethoxy, 1‐Butanol, nanomaterial synthesis, mean particle size, antibacterial activities, ethanol, acetone, 1‐ propanol, biocompatible ceria‐nanoparticle encapsulation, nano‐chitosan, Arctium lappa l., oleic acid, erythrocyte integrity, spectrophotometer measurement, haemoglobin release, mono dispersed beads shaped particle formation, X‐ray diffractometry, cubic crystalline structure, fluorescent live/dead staining, confocal laser scanning microscopy, multidrug resistance pathogens, size 26.2 nm, size 28.0 nm, size 48.8 nm, size 22.6 nm, CeO₂      

Green preparation of seaweed‐based silver nano‐liquid for cotton pathogenic fungi management

Silver nanoparticles (Ag NPs) were synthesised using the crude ethyl acetate extracts of Ulva lactuca and evaluated their bioefficacy against two crop‐damaging pathogens. The sets of lattice planes in the XRD spectrum for the Ag NPs were indexed to the 111, 200, 220 and 311 orientations and support the crystalline nature of the Ag NPs. The 3414 and 2968 cm⁻¹ peaks were observed in crude algal thallus extract and they were characteristic of terpenoids. Further, a peak at 1389 cm⁻¹ was observed as fatty acids. The marine macroalgae terpenoids and palmitic acid acted as reducing agent and stabiliser, respectively. The size (3 and 50 nm) and shape (spherical) of Ag NPs were recorded. The energy‐dispersive X‐ray spectroscopy analysis exemplified the presence of silver in its elemental nature. Moreover, U. lactuca Ag NPs were effective against two cotton phytopathogens namely Fusarium oxysporum f.sp. vasinfectum (FOV) and Xanthomonas campestris pv. malvacearum (XAM). The minimum inhibitory concentration was found to be 80.0 and 43.33 μg ml⁻¹ against FOV and XAM, respectively. Results confirmed the anti‐microbial activity of green nanoparticles against select pathogens and suggest their possible usage in developing antifungal agents for controlling destructive pathogens in a cotton agroecosystem.Inspec keywords: nanoparticles, biotechnology, antibacterial activity, silver, microorganisms, X‐ray chemical analysis, crops, X‐ray diffraction, cottonOther keywords: crude ethyl acetate extracts, crop‐damaging pathogens, lattice planes, XRD spectrum, crystalline nature, crude algal thallus, fatty acids, marine macroalgae terpenoids, palmitic acid, energy‐dispersive X‐ray spectroscopy analysis, elemental nature, cotton phytopathogens, green nanoparticles, destructive pathogens, cotton agroecosystem, green preparation, seaweed‐based silver nanoliquid, cotton pathogenic fungi management, silver nanoparticles, Ag NP, Ag     

Green synthesis of colloidal gold nanoparticles using latex from Hevea brasiliensis and evaluation of their in vitro cytotoxicity and genotoxicity

Latex extracted from Hevea brasiliensis tree has been used as a green alternative for preparing gold nanoparticles (Au NPs); however, no study evaluating the cytotoxic and genotoxic potential of Au NPs synthesised using H. brasiliensis has been published. The present study aimed to synthesise and characterise colloidal Au NPs using latex from H. brasiliensis and to evaluate their in vitro cytotoxicity and genotoxicity. Ideal conditions for the green synthesis of Au NPs were studied. In vitro cytotoxicity and genotoxicity of Au NPs in CHO‐K1 cells was also evaluated. Our findings indicated that the ideal synthesis conditions of pH, temperature, reduction time, and concentrations of latex and HAuCl₄ were 7.0, 85°C, 120 min, 3.3 mg/mL, and 5.0 mmol/L, respectively. LC50_{24 h} of Au NPs was 119.164 ± 5.31 μg/mL. Lowest concentration of Au NPs tested presented minimal cytotoxicity and genotoxicity. However, high concentrations of Au NPs promoted DNA damage and cell death via apoptosis. On the basis of these findings, the authors optimised the use of an aqueous solution of H. brasiliensis latex as a reducing/stabilising agent for the green synthesis of Au NPs. Low concentrations of these NPs are biocompatible in normal cell types, suggesting that these NPs may be used in biological applications.Inspec keywords: nanofabrication, biomedical materials, nanomedicine, colloids, pH, cellular biophysics, nanoparticles, gold, DNA, reduction (chemical), molecular biophysics, materials preparation, geneticsOther keywords: colloidal Au NPs, genotoxicity, green synthesis, in vitro cytotoxicity, Hevea brasiliensis, colloidal gold nanoparticle, latex concentrations, DNA damage, cell death, H. brasiliensis latex, normal cell types, Au     

Green synthesis of magnetically recoverable Fe3 O4 /HZSM‐5 and its Ag nanocomposite using Juglans regia L. leaf extract and their evaluation as catalysts for reduction of organic pollutants

In this work, an Fe₃ O₄ /HZSM‐5 nanocomposite was synthesised in the presence of Juglans regia L. leaf extract. Then, silver nanoparticles (Ag NPs) were immobilised on the surface of prepared magnetically recoverable HZSM‐5 using selected extract for reduction of Ag⁺ ions to Ag NPs and their stabilisation on the surface of the nanocomposite. The reduction of Ag⁺ ions occurs at room temperature within a few minutes. Characterisation of the prepared catalysts has been carried out using fourier transform infrared (FT‐IR), X‐ray diffraction, field‐emission scanning electron microscopy (FESEM), energy‐dispersive spectroscopy, Brunauer–Emmett–Teller method, and a vibrating sample magnetometer. According to the FESEM images of the nanocomposites, the average size of the Ag NPs on the Fe₃ O₄ /HZSM‐5 surface was >70 nm. The Ag/Fe₃ O₄ /HZSM‐5 nanocomposite was a highly active catalyst for the reduction of methyl orange and 4‐nitrophenol in aqueous medium. The utilisation of recycled catalyst for three times in the reduction process does not decrease its activity.Inspec keywords: silver, X‐ray chemical analysis, X‐ray diffraction, nanocomposites, reduction (chemical), nanofabrication, nanoparticles, transmission electron microscopy, catalysts, Fourier transform infrared spectra, iron compounds, field emission scanning electron microscopy, zeolites, magnetometry, particle sizeOther keywords: Ag‐Fe₃ O₄ , temperature 293 K to 298 K, green synthesis, catalyst material, 4‐nitrophenol reduction, methyl orange reduction, particle size, vibrating sample magnetometry, Brunauer–Emmett–Teller method, field‐emission scanning electron microscopy, X‐ray diffraction, FT‐IR spectroscopy, silver nanoparticles, Juglans regia L. leaf extract, organic pollutant reduction, magnetically recoverable nanocomposites, energy‐dispersive spectroscopy     

Biosynthesis of the CuO nanoparticles using Euphorbia Chamaesyce leaf extract and investigation of their catalytic activity for the reduction of 4‐nitrophenol

Through this study an eco‐friendly, simple, efficient, cheap and biocompatible approach to the biosynthesis and stabilisation of CuO nanoparticles (NPs) using the Euphorbia Chamaesyce leaf extract is presented. The CuO NPs were monitored and characterised by field emission scanning electron microscopy, energy dispersive X‐ray spectroscopy, Fourier transformed infrared spectroscopy, transmission electron microscope and UV‐visible spectroscopy. The biosynthesised CuO NPs showed good catalytic activity for the reduction of 4‐nitrophenol (4‐NP) in water during 180 s and reused 4 times without considerable loss of activity.Inspec keywords: copper compounds, nanoparticles, nanofabrication, catalysis, reduction (chemical), field emission electron microscopy, X‐ray chemical analysis, Fourier transform infrared spectra, transmission electron microscopy, ultraviolet spectra, visible spectraOther keywords: biosynthesis, CuO nanoparticles, Euphorbia Chamaesyce leaf extract, catalytic activity, 4‐nitrophenol reduction, nanoparticle stabilisation, field emission scanning electron microscopy, energy dispersive X‐ray spectroscopy, Fourier transformed infrared spectroscopy, transmission electron microscope, UV‐visible spectroscopy, CuO