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食品中残留农药检测技术的新进展 总被引:23,自引:2,他引:21
对食品中残留农药检测技术其新进展进行了综述。样品前处理中,固相萃取、超临界流体萃取、基质固相分散萃取得到了迅速发展和广泛应用。毛细管气相色谱、超临界流体色谱、液相色谱---质谱联用技术、酶免疫分析等已开始应用于食品的残留家药检测中。直接光谱技术和生物传感器的应用潜力很大。对食品中残留农药检测技术的发展进行了展望。 相似文献
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有机磷农药残留是重要的食品安全问题之一,有必要对食品中的有机磷农药进行监测。但植物源性食品成分复杂,进行有机磷农药检测前必须通过前处理进行提取和纯化。因此选择高效的有机磷农药残留前处理技术是非常重要的环节,已经成为当前研究者关注的重点。本文总结了有机磷农药分析的前处理方法的原理以及应用,包括传统提取法、加速溶剂萃取法、固相萃取法、QuEChERS法,介绍了有机磷农药的快速检测方法,包括分子印迹技术、酶抑制法、纳米材料富集法,并展望了对植物源性有机磷前处理技术的发展趋势。 相似文献
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酶联免疫技术及其在食品检测中的应用 总被引:6,自引:0,他引:6
说明了酶联免疫检测技术(ELISA)的原理、方法,主要论述了其在食品检测领域的应用,进行毒素、农药残留、食品微生物及其它微量元素的检测。 相似文献
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食品中苏丹红检测方法的研究进展 总被引:2,自引:0,他引:2
对食品中苏丹红检测方法及其研究进展进行了综述。评述了前处理方法如超声波辅助萃取、微波辅助萃取、凝胶渗透色谱、吸附薄层色谱、固相萃取、基质固相分散等技术在分析中的应用。概述了色谱法、质谱法、色-质联用法、光谱分析法、电化学分析法、酶联免疫吸附法、分子印迹技术用于食品中苏丹红检测的优势和局限性,并展望了未来该领域研究的发展趋势。 相似文献
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酶联免疫吸附分析及其在食品安全检测中的应用 总被引:12,自引:0,他引:12
主要介绍了酶联免疫分析(ELISA)测定的基本原理和分类,讨论了它在食品安全检测中的应用,如检测食品中毒素、病原微生物、农药残留、兽药残留、转基因食品成分等。 相似文献
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动物组织中赛庚啶竞争酶联免疫法的建立 总被引:1,自引:0,他引:1
建立快速检测动物组织中赛庚啶的竞争酶联免疫法。制备赛庚啶人工抗原作为包被原,使用辣根过氧化物酶标记的单克隆抗体,构建快速检测动物组织中赛庚啶的竞争酶联免疫法。结果表明,Logit/Log拟合标准曲线方程为Y=-2.051 8X-1.353 4,相关系数R为0.995 7,半数抑制浓度(IC50)为0.23μg/L,动物组织样本的检测限为0.3μg/kg,赛庚啶阳性样本的加标回收率为85.1%-114.5%,样本重复检测结果的变异系数为5.2%-9.6%。建立的竞争酶联免疫吸附方法具有较好的特异性、回收率和重复稳定性,可用于动物组织中赛庚啶残留的检测。 相似文献
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应用纯化的Bt1杀虫晶体蛋白质作为标准蛋白和免疫抗原 ,通过抗体 抗原 酶标抗体反应 ,建立了间接酶联免疫吸附测定法 (ELISA) ,以快速检测转基因抗虫玉米中的Bt1表达蛋白 .用建立的ELISA法对 4种进口玉米实物样品进行了测定 ,实验结果得到了免疫印迹分析的验证 ,并与进口试剂盒方法的定量分析结果相一致 相似文献
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QUAN WANG YING‐CHUN LIU JIAN LI WEI JIANG YONG‐JUN CHEN NING‐NING SONG 《Journal of food quality》2012,35(1):76-82
ABSTRACT
An indirect competitive enzyme‐linked immunosorbent assay with chemiluminescent (CL‐ELISA) detection for okadaic acid (OA) in mussel muscle was developed. A hybridoma cell line secreting monoclonal antibody against OA was established after immunization of BALB/c mice with artificially synthesized OA‐bovine serum albumin as antigen. Luminol solution was used as the substrate for horseradish peroxidase. The detection limit was 0.175 ng/g. The range of average fortified recovery was 91.8–102.6% when OA was spiked in mussel muscle at levels of 50, 160 and 800 µg/kg. The standard curve for OA showed good linearity over the concentration range of 0.08125–20 ng/mL. The cross‐reactivity with dinophysistoxin1 and saxitoxin was 51.82 and 0%, respectively. In a residue study, the results obtained by CL‐ELISA correlated well within those obtained using the commercial ABRAXIS kit (ABRAXIS, Warminster, PA). The developed method is therefore suitable for detecting the residues of OA in shellfish.PRACTICAL APPLICATIONS
Diarrhetic shellfish poisoning is a gastrointestinal syndrome that occurs in humans following the consumption of bivalve mollusks contaminated with OA with the main symptoms of diarrhea, nausea, vomiting and abdominal pain. OA widely exists at marine products and it is very important to develop a technique to monitor this toxin. In this study, a sensitive competitive indirect enzyme‐linked immunosorbent assay with chemiluminescence for determination of OA in mussel soft tissues was investigated. Chemiluminescent ELISA (CL‐ELISA) is a good alternative method for screening samples. This technique has the potential to improve the sensitivity of the immunoassays by at least two to three orders of magnitude compared with conventional colorimetric detection. 相似文献15.
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Junwei Liu Guozhen Fang Yan Zhang Wenjie Zheng Shuo Wang 《Journal of the science of food and agriculture》2009,89(1):80-87
BACKGROUND: An enzyme‐linked immunosorbent assay (ELISA) based on polyclonal antibodies with enhanced chemiluminescent (ECL) detection of sulfonamides in food samples has been optimised and characterised. The specificity of the assay was assessed by determining cross‐reactivities with a set of 16 sulfonamides. The aim of this study was to develop a method for determining sulfonamides with high sensitivity. RESULTS: The sensitivity of the developed ECL‐ELISA was higher than that of colorimetric ELISA. The sensitivities of five of the sulfonamides (sulfisozole, sulfathiazole, sulfameter, sulfamethoxypyridazine and sulfapyridine) ranged from 0.73 to 2.92 µg L?1, with limits of detection of 0.10–0.43 µg L?1. The coefficients of variation of intra‐assay and inter‐assay studies carried out over 5 days were mostly less than 10%. Recovery studies of chicken muscle and pig muscle were performed with simple and rapid extraction. Good recoveries (62.1–110.3%) were achieved and the results correlated well with those obtained using high‐performance liquid chromatography analysis. CONCLUSION: This study has provided an effective analytical technique for the rapid and reliable determination of residues of sulfisozole, sulfathiazole, sulfameter, sulfamethoxypyridazine and sulfapyridine in food samples with high sensitivity. To the authors' knowledge, this is the first report on chemiluminescent ELISA for sulfonamide analysis. Copyright © 2008 Society of Chemical Industry 相似文献
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研制能用于96 孔酶标板快速检测的微型丝网印刷电极,并建立克仑特罗的电化学分析方法。通过间接竞 争免疫反应与计时电流法相结合实现克仑特罗的残留量的定量测定。实验中考察并优化免疫反应与酶催化反应的 条件。结果表明:在优化的条件下,克仑特罗标准溶液质量浓度在0.025~2.0 μg/L范围内呈现良好线性关系(r为 0.999 2)。采用本实验方法检测猪肉和猪尿中克仑特罗的结果与商品化ELISA试剂盒一致。本方法检测限分别为 0.18 μg/L和0.05 μg/L,比ELISA试剂盒检测限低一个数量级。 相似文献
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免疫学技术在食品安全快速检测中的应用研究进展 总被引:1,自引:0,他引:1
随着人们对于食品安全问题的关注程度不断增加,食品安全快速检测方法得到了广泛应用。目前常用的食品安全快速检测技术包括免疫学技术、酶抑制技术、传感器技术、生物芯片技术等。免疫学检测技术具有灵敏度高、特异性强、方便、快速和经济等优点,在食品安全快速检测中发挥了重要的作用。免疫学技术在食品安全领域广泛应用的主要有免疫吸附法和免疫层析法两大体系,其中免疫吸附法以酶联免疫吸附检测法(enzyme-linked immunosorbent assay,ELISA)最常用,而免疫层析法则以胶体金免疫层析技术(colloidal gold immunochromatographic assay,GICA)为代表。本文介绍了免疫学技术在食品安全快速检测中应用的原理及特点,并对酶联免疫吸附检测技术和胶体金免疫层析技术近几年来在食品安全快速检测中的应用进展进行了综述。 相似文献
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近年来人们对果蔬及其产品的需求逐渐增加,随着生产、加工、销售规模的不断扩大,其产生的食品安全问题成为了人们普遍关注的焦点之一。本文阐述了果蔬在生产、流通等各环节中主要安全风险的来源,包括有机磷和氨基甲酸酯类农药残留、硝酸盐及亚硝酸盐等无机化合物超标、果蔬中有害微生物污染、病原菌毒素积累等,并对果蔬安全检测中快速检测技术(快检技术)的研究进展进行了综述,包括酶抑制法、酶联免疫法、传感器法、拉曼光谱检测法、试纸法、聚合酶链式反应法等。快检技术推进了果蔬安全检测的规范化和标准化,成为执法监管的重要依托,促进了果蔬产业安全持续的发展。 相似文献